IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Toc64, a new component of the protein translocon of chloroplasts. Toc64, a new component of the protein translocon of chloroplasts. J. Cell Biol. (0021-9525) 2000 Sohrt K., Soll J. Intact pea chloroplasts were incubated with 35S-labeled preSSU at 48C and 100 mM ATP for 5 min. Under these conditions the precursor engages both the Toc and the Tic complex but it cannot be fully translocated due to limitations in the ATP level. Pea chloroplast outer envelope membrane vesicles were isolated and cross-linked for 15 min on ice with CuCl2 or dithiobis[succinimidyl propionate] prior to coimmunoprecipitation. Figure 6. jsoll@bot.uni-kiel.de in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sohrt K., Soll J. Intact pea chloroplasts were incubated with 35S-labeled preSSU at 48C and 100 mM ATP for 5 min. Under these conditions the precursor engages both the Toc and the Tic complex but it cannot be fully translocated due to limitations in the ATP level. Pea chloroplast outer envelope membrane vesicles were isolated and cross-linked for 15 min on ice with CuCl2 or dithiobis[succinimidyl propionate] prior to coimmunoprecipitation. Figure 6. jsoll@bot.uni-kiel.de Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Cell Biol. (0021-9525) 2000 Toc64, a new component of the protein translocon of chloroplasts. Toc64, a new component of the protein translocon of chloroplasts. J. Cell Biol. (0021-9525) 2000 Sohrt K., Soll J. Intact pea chloroplasts were incubated with 35S-labeled preSSU at 48C and 100 mM ATP for 5 min. Under these conditions the precursor engages both the Toc and the Tic complex but it cannot be fully translocated due to limitations in the ATP level. Pea chloroplast outer envelope membrane vesicles were isolated and cross-linked for 15 min on ice with CuCl2 or dithiobis[succinimidyl propionate] prior to coimmunoprecipitation. Figure 6. jsoll@bot.uni-kiel.de in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Sohrt K., Soll J. Intact pea chloroplasts were incubated with 35S-labeled preSSU at 48C and 100 mM ATP for 5 min. Under these conditions the precursor engages both the Toc and the Tic complex but it cannot be fully translocated due to limitations in the ATP level. Pea chloroplast outer envelope membrane vesicles were isolated and cross-linked for 15 min on ice with CuCl2 or dithiobis[succinimidyl propionate] prior to coimmunoprecipitation. Figure 6. jsoll@bot.uni-kiel.de Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Cell Biol. (0021-9525) 2000 toc34_pea Translocase of chloroplast 34 34 kDa chloroplast outer envelope protein GTP-binding protein OEP34 GTP-binding protein IAP34 TOC34 IAP34 OEP34 protein protein pea Pisum sativum (Garden pea) Garden pea MASQQQTVREWSGINTFAPATQTKLLELLGNLKQEDVNSLTILVMGKGGVGKSSTVNSIIGERVVSISPFQSEGPRPVMVSRSRAGFTLNIIDTPGLIEGGYINDMALNIIKSFLLDKTIDVLLYVDRLDAYRVDNLDKLVAKAITDSFGKGIWNKAIVALTHAQFSPPDGLPYDEFFSKRSEALLQVVRSGASLKKDAQASDIPVVLIENSGRCNKNDSDEKVLPNGIAWIPHLVQTITEVALNKSESIFVDKNLIDGPNPNQRGKLWIPLIFALQYLFLAKPIEALIRRDIATETKPAWETRDVGDRK DIP protein Q41009 original sequence version: 60 DIP protein Q41009 original sequence version: 90 00D5E79735BF041F tic40_pea Protein TIC 40, chloroplastic TIC40 Translocon at the inner envelope membrane of chloroplasts 40 protein protein pea Pisum sativum (Garden pea) Garden pea MENLNLALVSSPKPLLLGHSSSKNVFSGRKSFTFGTFRVSANSSSSHVTRAASKSHQNLKSVQGKVNAHDFASISSSNGQETTSVGVSPQLSPPPPSTVGSPLFWIGIGVGFSALFSVVASRVKKYAMQQAFKSMMGQMNTQNNPFDSGAFSSGPPFPFPMPSASGPATPAGFAGNQSQATSTRSASQSTVTVDIPATKVEAAAPAPDINVKEEVEVKNEPKKSAFVDVSPEETVQKNAFERFKDVDESSSFKEARAPAEASQNGTPFKQGFGDSPSSPSERKSALSVDALEKMMEDPTVQQMVYPYLPEEMRNPSTFKWMMQNPEYRQQLEAMLNNMGGGTEWDSRMMDTLKNFDLNSPDVKQQFDQIGLSPQEVISKIMANPDVAMAFQNPRVQAAIMDCSQNPMSIVKYQNDKEVMDVFNKISELFPGVSGPP B9C18DE99BC93EC7 toc64_pea Translocon at the outer membrane of chloroplasts 64 TOC64 protein protein pea Pisum sativum (Garden pea) Garden pea MKSMASPSSQIWVILGLGLAGIYVLTRKLTQAVKEDFGAFLLKLKLLPPPPPAPPKAPHPLSSLNFAISDIFDIEGHVSTFGHPEWARTHEPASSTASAVSALVESGATCIGTTVVDELAYGISGENKHFGTPTNPAVPNRVPGGSSSGAAVAVAANFVDFSLGVDTSGGVRVPAGFCGILGFRPSHGAVSHVGIIPVSTSLDTVGWFAKDPDVLRRVGHILLQAPFVMQRNPRQIIIADDCFQHLNVPLDRTSQVVIKATEKLFGKQVLKHINFEDYISSKVSSLKACSIQKSNGVLKSSSLKLLANVMQSLQRHEFEHTHSEWMSIVKPDLHPAVSAQLHEKFEVSELEIENSKSVRSELRVAVNSLLKDEGVLVIPTVADPPPKLGGKEFLSHDYQSRALSLLSIASISGCCQVTVPLGFFDKNPVSVSLIARHGGDRFLLDTLKTMYTVLQEQADIAAPSKSSKSVVSKEQSAEISKEKGNQAYKDKQWQKAIGFYTEAIKLCGNNATYYSNRAQAYLELGSYLQAEEDCTTAISFDKKNVKAYFRRGTAREMLGYYKEAIDDFKYALVLEPTNKRAASSAERLRKLFQ 084E16C7E54E663B q9lkr1_pea protein protein pea Pisum sativum (Garden pea) Garden pea MDSQTLSSSSQFHEPNNLLNGVNGHGSDSDSDDGFVSGEDEAEPSTPILVYDAKSTVQVKEKEEESFDQESPRPIAKVTADDEDEAEEEEDDSQGVGLEKEGGGGGGKDVEEVKEDEVFVEANDKGFESVDSEGGDVVGEEINNGLREEGDGGTEIETVRSDSVVVEPVSVDNSGVGVVENGDGVVDNEKLTSGGDFVVDSLRVNPLVDGGVAVVGDEVKDEVSEIDGAVAPAPVASLDNSFEAIEKVGSRSVVDEVGSSFETIEKGDEVVVDDEVVGGDVEPSKVVDSGVEIEVDDNVAHEQLSDVVLTEKAGDVVVDENVGVGAKPDEVVDIGVDEGVAQRQVSDIAPAEKGEEISEVVSQSLEAAEDEINIENRVVEGGIESRVVEGGIESRVDDAVEGEVGSNVVEVEDGSNVDNVAEKDAVSNVDDAAEKDAVSNVDRVVEVEDESHVGNTVEGEARSNADHVLQVEDETHLDNAAVGEAKSNADRVVEVEDETPLDNAAVGEAESNVDPAVKVEDDTRFDNGAEGEAESNVDRVGEVEDDTHFDNAVEEEAESNVDRVVEVEDDTHFDNAVEEEADSNVDRVIEMDDGSHVEAAVDHHIDREIDDLLSDSKDESMIFGGSDSANKYLEELEKQIRDSESSQGDRIDGQIVTDSDEEDVSDEEGGSKELFDTATLAALLKAASGAGGEDGGGITLTAQDGSRLFSVERPAGLGPSLQTGKPAVRSIRPNLFAPSMSRAGTVVSDTDLSEEDKKKLEKLQEIRIKYLRVIQRLGFTTEESIAAQVLYRLTLVAGRQIGEMFSLDAAKESASRLEAEGRDDFAFSLNILVLGKTGVGKSATINSIFGETKTSFSAYGPATTSVTEIVGMVDGVEIRVFDTPGLKSSAFEQSYNRKVLSTVKKLTKKSPPDIVLYVDRLDLQTRDMNDLPMLRSVTSALGPTIWRNVIVTLTHAASAPPDGPSGSPLSYDVFVAQRSHIVQQAIGQAVGDLRLMNPNLMNPVSLVENHPSCRKNRDGQKVLPNGQSWKPLLLLLCYSMKILSEATNISKTQEAADNRRLFGFRSRAPPLPYLLSWLLQSRAHPKLPDQAGIDNGDSDIEMADLSDSDGEEGEDEYDQLPPFKPLKKSQIAKLNGEQRKAYLEEYDYRVKLLQKKQWREELKRMRDMKKRGKNGENDYMEEDEENGSPAAVPVPLPDMVLPQSFDSDNPAYRYRFLEPNSQLLTRPVLDTHSWDHDCGYDGVNIENSMAIINKFPAAVTVQVTKDKQDFSIHLDSSVAAKHGENGSTMAGFDIQNIGKQLAYIVRGETKFKNFKRNKTAAGVSVTFLGENVSTGVKLEDQIALGKRLVLVGSTGTVRSQNDSAYGANVEVRLREADFPVGQDQSSLSLSLVQWRGDLALGANFQSQISLGRSYKMAVRAGLNNKLSGQINVRTSSSDQLQIALIAILPVAKAIYKNFWPGVTENYSIY AA18A7C3DB5746C4 toc75_pea Protein TOC75, chloroplastic 75 kDa translocon at the outer-envelope membrane of chloroplasts Import intermediate-associated protein of 75 kDa 75 kDa chloroplast outer envelope protein TOC75 IAP75 OEP75 protein protein pea Pisum sativum (Garden pea) Garden pea MRTSVIPNRLTPTLTTHPSRRRNDHITTRTSSLKCHLSPSSGDNNDSFNSSLLKTISTTVAVSSAAASAFFLTGSLHSPFPNFSGLNAAAGGGAGGGGGGSSSSGGGGGGWFNGDEGSFWSRILSPARAIADEPKSEDWDSHELPADITVLLGRLSGFKKYKISDILFFDRNKKSKVETQDSFLDMVSLKPGGVYTKAQLQKELESLATCGMFEKVDMEGKTNADGSLGLTISFAESMWERADRFRCINVGLMGQSKPVEMDPDMSEKEKIEFFRRQEREYKRRISSARPCLLPTSVHEEIKDMLAEQGRVSARLLQKIRDRVQSWYHEEGYACAQVVNFGNLNTREVVCEVVEGDITKLSIQYLDKLGNVVEGNTEGPVVQRELPKQLLPGHTFNIEAGKQALRNINSLALFSNIEVNPRPDEMNEGSIIVEIKLKELEQKSAEVSTEWSIVPGRGGRPTLASLQPGGTITFEHRNLQGLNRSLTGSVTTSNFLNPQDDLAFKMEYAHPYLDGVDNPRNRTLRVSCFNSRKLSPVFTGGPGVDEVPSIWVDRAGVKANITENFSRQSKFTYGLVMEEIITRDESNHICSNGQRVLPNGAISADGPPTTLSGTGIDRMAFLQANITRDNTRFVNGTIVGSRNMFQVDQGLGVGSNFPFFNRHQLTVTKFLQLMSVEEGAGKSPPPVLVLHGHYGGCVGDLPSYDAFTLGGPYSVRGYNMGEIGAARNILELAAEIRIPIKGTHVYAFAEHGTDLGSSKDVKGNPTVVYRRMGQGSSYGAGMKLGLVRAEYAVDHNSGTGAVFFRFGERF AFE51AE75F0617C5 ti110_pea Protein TIC110, chloroplastic TIC110 IEP110 Chloroplast inner envelope protein, 110 kDa IAP100 Translocon at the inner envelope membrane of chloroplasts 110 protein protein pea Pisum sativum (Garden pea) Garden pea MNPSTLKPSHTHPSLLLPAPSPLRTQRRRFRVSLPRCSSDTNNPASSSSPPQRPPKELNGIEILVDKLSSPARLATSAVIVAGAVAAGYGLGSRFGGSRNAALGGAVALGAAGGAAAYALNAAAPQVAAVNLHNYVAGFDDPSILTREDIEVIANKYGVSKQDEAFKAEICDIYSEFVSSVIPPGGEELKGDEVDKIVNFKSSLGLDDPDAAAVHMEIGRKLFRQRLEVGDREGGVEQRRAFQKLIYVSNIVFGDASSFLLPWKRVFKVTESQVEVAIRDNAQRLYASKLKSVGRDFDLGKLVTLKETQSLCCLSDELAENLFREHARKLVEENISVALGILKSRTRAVPGVSQVVEEIEKVLAFNDLLISFKNHSDIDRLARGVGPVSLVGGEYDADRKIEDLKLLYRAYVSDALSSGRMEDNKFAALNQLKNIFGLGKREAEAILLDITRKVYRKRLGQTVSSGELEMADSKAAFLQNLCDELHFDPQKASELHEEIYRQKLQQCVADGELTDENVAALLKLRVMLCVPQQTVEAAHAEICGNLFEKIVKDAIASGVDGYDDETKKSVRKAAHGLRLTKETALSIASKAVRKMFITYVKRSRSAKGNGESAKELKKLIAFNTLVVTKLVEDIKGESPDVKIEEPKIEEPEEIRESEEYEMRITSDTQENKTGQRACRKDGKAWSDRITLKDDLPEKDRADLYKTFLTYCLTGDVVRIPFGVEIKKKKDDTEYIYLNQLGGILGLTGKVIMDVHRGLAEQAFRKQAEVLLADGQLTKARVEQLGKMQKEIGLSQEYAQKIIKNITTTKMAAAIETAVTQGKLNMKQIRELKESNVDLDSMVSVSLRETIFKKTVGDIFSSGTGEFDEEEVYEKIPLDLNINKEKARGVVCELAQNRLSNSLIQAVALLRQRNHKGVVFSLNNLLACDKAVPSQTLSWEVSEELSDLYTIYLKSDPSPEKLSRLQYLLGINDSTAAALRDSEDSLLETAEEEKFVF This sequence has been withdrawn from Uniprot. E36649FF7A0C2680 E36649FF7A0C2680 toc complex 2 7 unspecified role unspecified role neutral component neutral component pea Pisum sativum (Garden pea) Garden pea 6 unspecified role unspecified role neutral component neutral component pea Pisum sativum (Garden pea) Garden pea 5 unspecified role unspecified role neutral component neutral component pea Pisum sativum (Garden pea) Garden pea 3 unspecified role unspecified role neutral component neutral component pea Pisum sativum (Garden pea) Garden pea physical association physical association toc complex-1 1 7 unspecified role unspecified role unspecified role unspecified role pea Pisum sativum (Garden pea) Garden pea 8 unspecified role unspecified role unspecified role unspecified role pea Pisum sativum (Garden pea) Garden pea 4 unspecified role unspecified role unspecified role unspecified role pea Pisum sativum (Garden pea) Garden pea 6 unspecified role unspecified role unspecified role unspecified role pea Pisum sativum (Garden pea) Garden pea 5 unspecified role unspecified role unspecified role unspecified role pea Pisum sativum (Garden pea) Garden pea 3 unspecified role unspecified role unspecified role unspecified role pea Pisum sativum (Garden pea) Garden pea association association The identity of the pre SSU protein used in this assay could not be verified. The authors provide no information concerning its origin.