IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Assembly of plant Shaker-like K(out) channels requires two distinct sites of the channel alpha-subunit. Assembly of plant Shaker-like K(out) channels requires two distinct sites of the channel alpha-subunit. Biophys. J. (0006-3495) 2004 Dreyer I., Poree F., Schneider A., Mittelstadt J., Bertl A., Sentenac H., Thibaud JB., Mueller-Roeber B. The authors do not provide the coordinates of the construct SKOR ABCDEF- used in Figure 4B , therefore interactions involving this construct culd not be curated. dreyer@rz.uni-potsdam.de yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Dreyer I., Poree F., Schneider A., Mittelstadt J., Bertl A., Sentenac H., Thibaud JB., Mueller-Roeber B. The authors do not provide the coordinates of the construct SKOR ABCDEF- used in Figure 4B , therefore interactions involving this construct culd not be curated. dreyer@rz.uni-potsdam.de Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Biophys. J. (0006-3495) 2004 gork_arath Potassium channel GORK GORK At5g37500 MPA22.4 Guard cell outward rectifying K(+) channel protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGRLRRRQEIIDHEEEESNDDVSSRRGKLSLAETFRWLDSSEHRRIETDGHNDYKYIIHPKNRWYKAWEMFILVWAIYSSLFTPMEFGFFRGLPERLFVLDIVGQIAFLVDIVLQFFVAYRDTQTYRTVYKPTRIAFRYLKSHFLMDFIGCFPWDLIYKASGKHELVRYLLWIRLFRVRKVVEFFQRLEKDTRINYLFTRILKLLFVEVYCTHTAACIFYYLATTLPPENEGYTWIGSLKLGDYSYENFREIDLWKRYTTALYFAIVTMATVGYGDIHAVNLREMIFVMIYVSFDMVLGAYLIGNITALIVKGSNTERFRDKMNDLISFMNRKKLGRDLRSQITGHVRLQYDSHYTDTVMLQDIPASIRAKIAQLLYLPYIKKVPLFKGCSTEFINQIVIRLHEEYFLPGEVITEQGNVVDHLYFVCEGLLEALVTKTDGSEESVTLLGPHTSFGDISIICNISQPFTVRVCELCHLLRLDKQSFSNILEIYFHDGRTILNNIMEEKESNDRIKKLESDIVIHIGKQEAELALKVNSAAFQGDFYQLKSLIRSGADPNKTDYDGRSPLHLAACRGYEDITLFLIQEGVDVNLKDKFGHTPLFEAVKAGQEGVIGLLVKEGASFNLEDSGNFLCTTVAKGDSDFLKRLLSSGMNPNSEDYDHRTPLHVAASEGLFLMAKMLVEAGASVISKDRWGNSPLDEARLCGNKKLIKLLEDVKNAQSSIYPSSLRELQEERIERRKCTVFPFHPQEAKEERSRKHGVVVWIPSNLEKLIVTAAKELGLSDGASFVLLSEDQGRITDIDMISDGHKLYMISDTTDQT 65F57190056A9AE4 skor_arath Potassium channel SKOR SKOR At3g02850 F13E7.21 Stelar K(+) outward rectifying channel protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGGSSGGGVSYRSGGESDVELEDYEVDDFRDGIVESRGNRFNPLTNFLGLDFAGGSGGKFTVINGIRDISRGSIVHPDNRWYKAWTMFILIWALYSSFFTPLEFGFFRGLPENLFILDIAGQIAFLVDIVLTFFVAYRDSRTYRMIYKRSSIALRYLKSTFIIDLLACMPWDIIYKAAGEKEEVRYLLLIRLYRVHRVILFFHKMEKDIRINYLFTRIVKLIFVELYCTHTAACIFYYLATTLPASQEGYTWIGSLKLGDYSYSKFREIDLWTRYTTSMYFAVVTMATVGYGDIHAVNMREMIFAMVYISFDMILGAYLIGNMTALIVKGSKTERFRDKMADIMRYMNRNKLGRNIRGQITGHLRLQYESSYTEAAVLQDIPVSIRAKIAQTLYLPYIEKVPLFRGCSSEFINQIVIRLHEEFFLPGEVIMEQGSVVDQLYFVCHGVLEEIGITKDGSEEIVAVLQPDHSFGEISILCNIPQPYTVRVAELCRILRLDKQSFMNILEIFFHDGRRILNNLLEGKESNVRIKQLESDITFHISKQEAELALKLNSAAFYGDLYQLKSLIRAGGDPNKTDYDGRSPLHLAASRGYEDITLYLIQESVDVNIKDKLGSTPLLEAIKNGNDRVAALLVKEGATLNIENAGTFLCTVVAKGDSDFLKRLLSNGIDPNSKDYDHRTPLHVAASEGFYVLAIQLVEASANVLAKDRWGNTPLDEALGCGNKMLIKLLEDAKNSQISSFPSGSKEPKDKVYKKKCTVYFSHPGDSKEKRRRGIVLWVPRSIEELIRTAKEQLNVPEASCVLSEDEAKIIDVDLISDGQKLYLAVET C6C7B161C8904D03 skor-skor-1 1 3 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 3 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association The authors concluded that the cNBD region and " region B (representing a 33 amino acid stretch just upstream of the cNBD)" plays an important role in triggering the physical interactions of the Kout channel C-termini". Figure 4B gork-skor-1 1 3 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association Figure 4A gork-gork-1 1 2 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association Figure 4A skor-skor-2 1 3 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 3 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association Intermolecular interaction of SKOR and GORK, and different SKOR C-terminal fragments tested in the yeast two-hybrid system. Figure 4A