IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Binding stoichiometry and kinetics of the interaction of a human anthrax toxin receptor, CMG2, with protective antigen. Binding stoichiometry and kinetics of the interaction of a human anthrax toxin receptor, CMG2, with protective antigen. J. Biol. Chem. (0021-9258) 2004 Wigelsworth DJ., Krantz BA., Christensen KA., Lacy DB., Juris SJ., Collier RJ. fig 2 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Wigelsworth DJ., Krantz BA., Christensen KA., Lacy DB., Juris SJ., Collier RJ. fig 2 Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Biol. Chem. (0021-9258) 2004 Binding stoichiometry and kinetics of the interaction of a human anthrax toxin receptor, CMG2, with protective antigen. Binding stoichiometry and kinetics of the interaction of a human anthrax toxin receptor, CMG2, with protective antigen. J. Biol. Chem. (0021-9258) 2004 Wigelsworth DJ., Krantz BA., Christensen KA., Lacy DB., Juris SJ., Collier RJ. fig 2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Wigelsworth DJ., Krantz BA., Christensen KA., Lacy DB., Juris SJ., Collier RJ. fig 2 Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Biol. Chem. (0021-9258) 2004 Binding stoichiometry and kinetics of the interaction of a human anthrax toxin receptor, CMG2, with protective antigen. Binding stoichiometry and kinetics of the interaction of a human anthrax toxin receptor, CMG2, with protective antigen. J. Biol. Chem. (0021-9258) 2004 Wigelsworth DJ., Krantz BA., Christensen KA., Lacy DB., Juris SJ., Collier RJ. fig 2 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Wigelsworth DJ., Krantz BA., Christensen KA., Lacy DB., Juris SJ., Collier RJ. fig 2 Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Biol. Chem. (0021-9258) 2004 antr2_human Anthrax toxin receptor 2 Capillary morphogenesis gene 2 protein ANTXR2 CMG2 protein protein human Homo sapiens Human MVAERSPARSPGSWLFPGLWLLVLSGPGGLLRAQEQPSCRRAFDLYFVLDKSGSVANNWIEIYNFVQQLAERFVSPEMRLSFIVFSSQATIILPLTGDRGKISKGLEDLKRVSPVGETYIHEGLKLANEQIQKAGGLKTSSIIIALTDGKLDGLVPSYAEKEAKISRSLGASVYCVGVLDFEQAQLERIADSKEQVFPVKGGFQALKGIINSILAQSCTEILELQPSSVCVGEEFQIVLSGRGFMLGSRNGSVLCTYTVNETYTTSVKPVSVQLNSMLCPAPILNKAGETLDVSVSFNGGKSVISGSLIVTATECSNGIAAIIVILVLLLLLGIGLMWWFWPLCCKVVIKDPPPPPAPAPKEEEEEPLPTKKWPTVDASYYGGRGVGGIKRMEVRWGDKGSTEEGARLEKAKNAVVKIPEETEEPIRPRPPRPKPTHQPPQTKWYTPIKGRLDALWALLRRQYDRVSLMRPQEGDEVCIWECIEKELTA B9F66CCE7A13F807 pag_bacan Protective antigen PA-83 Anthrax toxins translocating protein pagA pag pXO1-110 BXA0164 GBAA_pXO1_0164 protein protein bacan Bacillus anthracis MKKRKVLIPLMALSTILVSSTGNLEVIQAEVKQENRLLNESESSSQGLLGYYFSDLNFQAPMVVTSSTTGDLSIPSSELENIPSENQYFQSAIWSGFIKVKKSDEYTFATSADNHVTMWVDDQEVINKASNSNKIRLEKGRLYQIKIQYQRENPTEKGLDFKLYWTDSQNKKEVISSDNLQLPELKQKSSNSRKKRSTSAGPTVPDRDNDGIPDSLEVEGYTVDVKNKRTFLSPWISNIHEKKGLTKYKSSPEKWSTASDPYSDFEKVTGRIDKNVSPEARHPLVAAYPIVHVDMENIILSKNEDQSTQNTDSQTRTISKNTSTSRTHTSEVHGNAEVHASFFDIGGSVSAGFSNSNSSTVAIDHSLSLAGERTWAETMGLNTADTARLNANIRYVNTGTAPIYNVLPTTSLVLGKNQTLATIKAKENQLSQILAPNNYYPSKNLAPIALNAQDDFSSTPITMNYNQFLELEKTKQLRLDTDQVYGNIATYNFENGRVRVDTGSNWSEVLPQIQETTARIIFNGKDLNLVERRIAAVNPSDPLETTKPDMTLKEALKIAFGFNEPNGNLQYQGKDITEFDFNFDQQTSQNIKNQLAELNATNIYTVLDKIKLNAKMNILIRDKRFHYDRNNIAVGADESVVKEAHREVINSSTEGLLLNIDKDIRKILSGYIVEIEDTEGLKEVINDRYDMLNISSLRQDGKTFIDFKKYNDKLPLYISNPNYKVNVYAVTKENTIINPSENGDTSTNGIKKILIFSKKGYEIG DIP protein P13423 original sequence version: 106 DIP protein P13423 original sequence version: 91 DIP protein P13423 original sequence version: 93 DIP protein P13423 original sequence version: 110 DIP protein P13423 original sequence version: 124 DIP protein P13423 original sequence version: 150 3AE1EFBF48FAA03F paga-antxr2-2 2 5 unspecified role unspecified role neutral component neutral component pa-83 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain bacan Bacillus anthracis 4 unspecified role unspecified role neutral component neutral component vwfa binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) physical association physical association stoichiometry 1:1 paga-antxr2-3 1 4 unspecified role unspecified role neutral component neutral component vwfa binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P58335:p.Arg40Cys mutation mutation certain certain sequence position certain certain certain sequence position certain R C Wed May 02 14:59:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg40cys' Tue Jun 30 16:45:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg40Cys' fluorophore CMG2 is modified for AF546 fluorophore attachment : R40C alexa 546 label alexa 546 label certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role neutral component neutral component pa-83 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-490554]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 767-767, sequence length 764 [EBI-490554]30-767 P13423:p.Glu762Cys mutation mutation certain certain sequence position certain certain certain sequence position certain E C Wed May 02 18:16:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu762cys' Tue Jun 30 19:58:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu762Cys' fluorophore PA-83 is modified for AF488 fluorophore attachment : E762C (on PAGA sequence) alexa 488 label alexa 488 label certain certain sequence position certain certain certain sequence position certain bacan Bacillus anthracis direct interaction direct interaction stoechiometry 1:1 or 7:7 At 2mM Mg2+, ka= 5.3E4 M-1 s-1 and kd = 9.2E-6 s-1 paga-antxr2-4 1 4 unspecified role unspecified role neutral component neutral component vwfa binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P58335:p.Arg40Cys mutation mutation certain certain sequence position certain certain certain sequence position certain R C Wed May 02 20:06:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg40cys' Tue Jun 30 17:55:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg40Cys' fluorophore CMG2 (antrx2) is modified for AF546 fluorophore attachment : R40C alexa 546 label alexa 546 label certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role neutral component neutral component P13423:p.Glu762Cys mutation mutation certain certain sequence position certain certain certain sequence position certain E C Wed May 02 16:26:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu762cys' Tue Jun 30 16:12:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu762Cys' pa-63 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain fluorophore PA-63 is modified for AF488 fluorophore attachment : E762C (on PAGA sequence) alexa 488 label alexa 488 label certain certain sequence position certain certain certain sequence position certain bacan Bacillus anthracis direct interaction direct interaction paga-antxr2-5 3 4 unspecified role unspecified role prey prey vwfa binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role bait bait pa-83 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain bacan Bacillus anthracis direct interaction direct interaction At 1 mM Ca2+, ka= 1.7.10E5 M-1 s-1 and kd= 7.10E-5 s-1 paga-paga-2 1 5 unspecified role unspecified role neutral component neutral component P13423:p.Glu762Cys mutation mutation certain certain sequence position certain certain certain sequence position certain E C Wed May 02 16:08:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu762cys' Tue Jun 30 16:40:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu762Cys' pa-63 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain fluorophore alexa 546 label alexa 546 label certain certain sequence position certain certain certain sequence position certain bacan Bacillus anthracis 5 unspecified role unspecified role neutral component neutral component pa-63 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P13423:p.Glu762Cys mutation mutation certain certain sequence position certain certain certain sequence position certain E C Wed May 02 17:14:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu762cys' Tue Jun 30 20:21:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu762Cys' fluorophore alexa 488 label alexa 488 label certain certain sequence position certain certain certain sequence position certain bacan Bacillus anthracis direct interaction direct interaction A mixture of fluorescently labeled PA-63 on E762C is used (donor AF488 and acceptor) to measure heptamerization Addition of anthrax lethal factor (LF) VWFA protein fragment (34-296) is required for PA-63 heptamerization heptamerization reaction profiles fit to a second order rate with rate constants of 8.8 x 10-4 and 7.8 x 10-4 s-1 in the presence and absence of CMG2, respectively. paga-antxr2-7 1 5 unspecified role unspecified role neutral component neutral component undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:06:50 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'glu762cys-x' pa-83-x binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1179681]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 767-767, sequence length 764 [EBI-1179681]30-767 fluorophore-x PA-83 is modified for AF488 fluorophore attachment : E762C (on PAGA sequence) alexa 488 label alexa 488 label certain certain sequence position certain certain certain sequence position certain bacan Bacillus anthracis 5 unspecified role unspecified role neutral component neutral component pa-83 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1179705]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 767-767, sequence length 764 [EBI-1179705]30-767 P13423:p.Glu762Cys mutation mutation certain certain sequence position certain certain certain sequence position certain E C Wed May 02 19:43:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu762cys' Tue Jun 30 17:20:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu762Cys' fluorophore PA-83 is modified for AF488 fluorophore attachment : E762C (on PAGA sequence) alexa 488 label alexa 488 label certain certain sequence position certain certain certain sequence position certain bacan Bacillus anthracis 4 unspecified role unspecified role neutral component neutral component vwfa binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P58335:p.Arg40Cys mutation mutation certain certain sequence position certain certain certain sequence position certain R C Wed May 02 15:58:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg40cys' Tue Jun 30 18:22:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg40Cys' fluorophore CMG2 is modified for AF546 fluorophore attachment : R40C alexa 546 label alexa 546 label certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 4 unspecified role unspecified role neutral component neutral component vwfa-x binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:04:11 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'arg40cys-x' fluorophore-x CMG2 is modified for AF546 fluorophore attachment : R40C alexa 546 label alexa 546 label certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction stoechiometry 1:1 or 7:7 At 2mM Mg2+, ka= 5.3E4 M-1 s-1 and kd = 9.2E-6 s-1 stoechiometry 1:1 or 7:7