MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Identification of an organelle-specific myosin V receptor. Identification of an organelle-specific myosin V receptor. J. Cell Biol. (0021-9525) 2003 imex curation Ishikawa K., Catlett NL., Novak JL., Tang F., Nau JJ., Weisman LS. lois-weisman@uiowa.edu Only protein-protein interactions imex curation in vivo in vivo 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Ishikawa K., Catlett NL., Novak JL., Tang F., Nau JJ., Weisman LS. lois-weisman@uiowa.edu J. Cell Biol. (0021-9525) 2003 IMEx myo2_yeast Myosin-2 MYO2 CDC66 YOR326W O6167 Class V unconventional myosin MYO2 Type V myosin heavy chain MYO2 Cell division control protein 66 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFEVGTRCWYPHKELGWIGAEVIKNEFNDGKYHLELQLEDDEIVSVDTKDLNNDKDQSLPLLRNPPILEATEDLTSLSYLNEPAVLHAIKQRYSQLNIYTYSGIVLIATNPFDRVDQLYTQDMIQAYAGKRRGELEPHLFAIAEEAYRLMKNDKQNQTIVVSGESGAGKTVSAKYIMRYFASVEEENSATVQHQVEMSETEQKILATNPIMEAFGNAKTTRNDNSSRFGKYLEILFDKDTSIIGARIRTYLLERSRLVYQPPIERNYHIFYQLMAGLPAQTKEELHLTDASDYFYMNQGGDTKINGIDDAKEYKITVDALTLVGITKETQHQIFKILAALLHIGNIEIKKTRNDASLSADEPNLKLACELLGIDAYNFAKWVTKKQIITRSEKIVSNLNYSQALVAKDSVAKFIYSALFDWLVENINTVLCNPAVNDQISSFIGVLDIYGFEHFEKNSFEQFCINYANEKLQQEFNQHVFKLEQEEYVKEEIEWSFIEFNDNQPCIDLIENKLGILSLLDEESRLPAGSDESWTQKLYQTLDKSPTNKVFSKPRFGQTKFIVSHYALDVAYDVEGFIEKNRDTVSDGHLEVLKASTNETLINILEGLEKAAKKLEEAKKLELEQAGSKKPGPIRTVNRKPTLGSMFKQSLIELMNTINSTNVHYIRCIKPNADKEAWQFDNLMVLSQLRACGVLETIRISCAGFPSRWTFEEFVLRYYILIPHEQWDLIFKKKETTEEDIISVVKMILDATVKDKSKYQIGNTKIFFKAGMLAYLEKLRSNKMHNSIVMIQKKIRAKYYRKQYLQISQAIKYLQNNIKGFIIRQRVNDEMKVNCATLLQAAYRGHSIRANVFSVLRTITNLQKKIRKELKQRQLKQEHEYNAAVTIQSKVRTFEPRSRFLRTKKDTVVVQSLIRRRAAQRKLKQLKADAKSVNHLKEVSYKLENKVIELTQNLASKVKENKEMTERIKELQVQVEESAKLQETLENMKKEHLIDIDNQKSKDMELQKTIENNLQSTEQTLKDAQLELEDMVKQHDELKEESKKQLEELEQTKKTLVEYQTLNGDLQNEVKSLKEEIARLQTAMSLGTVTTSVLPQTPLKDVMGGGASNFNNMMLENSDLSPNDLNLKSRSTPSSGNNHIDSLSVDRENGVNATQINEELYRLLEDTEILNQEITEGLLKGFEVPDAGVAIQLSKRDVVYPARILIIVLSEMWRFGLTKQSESFLAQVLTTIQKVVTQLKGNDLIPSGVFWLANVRELYSFVVFALNSILTEETFKNGMTDEEYKEYVSLVTELKDDFEALSYNIYNIWLKKLQKQLQKKAINAVVISESLPGFSAGETSGFLNKIFANTEEYTMDDILTFFNSIYWCMKSFHIENEVFHAVVTTLLNYVDAICFNELIMKRNFLSWKRGLQLNYNVTRLEEWCKTHGLTDGTECLQHLIQTAKLLQVRKYTIEDIDILRGICYSLTPAQLQKLISQYQVADYESPIPQEILRYVADIVKKEAALSSSGNDSKGHEHSSSIFITPETGPFTDPFSLIKTRKFDQVEAYIPAWLSLPSTKRIVDLVAQQVVQDGH 1F7E2887C1E59D54 1F7E2887C1E59D54 vac17_yeast vacuole-related protein 17 YCL063W YCL63W/YCL62W VAC17 Vacuole-specific MYO2 receptor VAC17 protein protein yeast Saccharomyces cerevisiae Baker's yeast MATQALEDITERLLIRSQEAILQLDLWIQRQQRSSICQTTDQESLDKLSQQYNQYMSQLNSLYVRSESVRDKLSKEQQRRLITEDNEHQRIEDLVREFQDITLRLNELATVPNEAPNDSPQSQSTRSSLGSFQPRPLKIIERQRLCMVTPSKPPKKSVGFNPINEVDCPSKTNSLPCSPKKQPARNRTLRAAKSHDTGLNKSKKPSSSDTYESFFKNRQRLSLTFFDEMDDEDFDSDQDTIILPNISTPPHVGVTAKGAEFEPLRRYNSHESILSNKPAPSKSLNLGSFSASFFRPSNPTFGTSISNVQVNCHPTVAATMAPSRNGPRISSSKALLSSFIARSDTHTVKENNTNLKHASFMDKFNSSLSTISESFQSKRGRKNKGMNEERISNHNVAQEQKNNMDISVSIEELQDALNTELLF 7F14A3A0BFE9571B 7F14A3A0BFE9571B myo2-vac17-1 1 3 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Stoichiometry: 1.0 2 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Stoichiometry: 1.0 physical association physical association Fig. 3A mint myo2-vac17-2 1 3 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Stoichiometry: 1.0 2 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P19524:p.Gly1248Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G D Wed May 02 16:04:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly1248asp' Tue Jun 30 18:28:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly1248Asp' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P19524:p.Asp1297Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D N Wed May 02 15:37:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1297asn' Tue Jun 30 15:39:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1297Asn' P19524:p.Leu1301Pro mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L P Wed May 02 16:34:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu1301pro' Tue Jun 30 19:19:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1301Pro' P19524:p.Asn1304Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N D Wed May 02 18:15:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn1304asp' Tue Jun 30 17:16:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn1304Asp' P19524:p.Asn1304Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N S Wed May 02 19:31:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn1304ser' Tue Jun 30 19:24:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn1304Ser' Stoichiometry: 1.0 physical association physical association mint Fig. 3D myo2-vac17-3 1 3 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Stoichiometry: 1.0 2 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Stoichiometry: 1.0 physical association physical association Fig. 3C mint myo2-vac17-4 1 3 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Stoichiometry: 1.0 2 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P19524:p.Gly1248Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G D Wed May 02 19:22:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly1248asp' Tue Jun 30 18:24:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly1248Asp' P19524:p.Gly1248Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G N Wed May 02 20:18:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly1248asn' Tue Jun 30 16:10:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly1248Asn' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Stoichiometry: 1.0 physical association physical association Fig. 4B mint myo2-vac17-5 1 3 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P25591:p.Ile140Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I V Wed May 02 15:56:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile140val' Tue Jun 30 16:14:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile140Val' Stoichiometry: 1.0 2 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P19524:p.Asn1304Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N S Wed May 02 14:51:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn1304ser' Tue Jun 30 19:43:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn1304Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P19524:p.Gly1248Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G D Wed May 02 20:02:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly1248asp' Tue Jun 30 19:55:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly1248Asp' Stoichiometry: 1.0 physical association physical association Fig. 3D mint