IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The conserved glutamine-rich region of chick csal1 and csal3 mediates protein interactions with other spalt family members. Implications for Townes-Brocks syndrome. The conserved glutamine-rich region of chick csal1 and csal3 mediates protein interactions with other spalt family members. Implications for Townes-Brocks syndrome. J. Biol. Chem. (0021-9258) 2003 Sweetman D., Smith T., Farrell ER., Chantry A., Munsterberg A. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sweetman D., Smith T., Farrell ER., Chantry A., Munsterberg A. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Biol. Chem. (0021-9258) 2003 q9df77_chick SAL1 protein protein chick Gallus gallus (Chicken) Chicken MSRRKQAKPQHFQSDPDLASLSQRNGDTEKGQGNRTTKNKDAHVCGRCCAEFFELSDLLQHKKNCTKNQLVLIVNENPASPPETFPPRSPSDNPDEQMNDTVNNTDQVDCSDLSERNKLDKEESMDVEASSINNSSSSSKSVNNSITSSNSSTMGTSAVTTSLPHIGDLTTLGNFSVINSNVIIENLQSTKVAVAQFSQEARCNGASNNKLAVPALMEQLLALQQQQIHQLQLIEQIRHQILLLASQNTDMPTSSSPSQGTLRASANPLSTLSSHLSQQLAAAAGLAQSLASQSASISGVKQLPPIQLPQSNPGSTLIPSSSGSSPNINILAAAVTTPSSEKVASSIGGSQLSNPPVSASSSPAFAISSLLSPASNPLLPQPTPSNSIFSSPLSNIGTPAEDLNSLTALAQQRKSKPPNVTAFEAKSNSDEAFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEHLDNIPTSTGIPYGMSIPPEKPVTSWLDSKPVLSTLTTSVGLPLPPTIPSLTPFIKTEEPQPIPISHPSASPPCSVKSDSGTADPTSKISNGLSDEVEAGALPTSNGKMEENPQNSNSVANMSSSVSSPAADSGSGSIATFTNPLMPLMSEQFKAKFPFGGLLDSTPASETSKLQQLVENIDKKATDPNECIICHRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHYSVHRAMPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTPVTENYPESMESDTGSFDDKNFDDLDNFSDENMEDCPDSSVPDTPKSADASQDSLSSSPLPLEMSSIAALENQMKMINAGLAEQLQASLKSVENGSVEGDVLTNDSSSVGGDMESQSAGSPAVSESTSSMQALSPSNSTNDYHKSPSIEEKPVRALASEFANGLSPTPANSGALDLTSSNTDKMIKEESLSMLFPFRDRGKFKNTACDICGKTFACQSALDIHYRSHTKERPFICTVCNRGFSTKGNLKQHMLTHQMRDLPSQLFEPNSSIGPNQNSSVMPANSLSSLIKTEVNGFVHSSSQDGKETPSALAASGPLSSATSPVLLPALPRRTPKQHYCNTCGKTFSSSSALQIHERTHTGEKPFACTICGRAFTTKGNLKVHMGTHMWNSTPARRGRRLSVDGPMTFLGGNPVKFPEMFQKDLAARSGNGDPSSFWNQYAAALSNGLAMKTNEISVIQNGGIPPPPGGLGNGGSSPISGLTGSLEKLPSSEPNAPLAGLEKMASNENGTNFRFTRFVEDNKEIVTN 81810CC16336298E q90zw5_chick SAL2 protein protein chick Gallus gallus (Chicken) Chicken MSRRKQAKPQHLKSDEELQAEVVSEHAVPGEGADDGDSGNESRSGSEETNVCEKCCAEFFKWTDFLEHKKNCTKNPLVLIVNEDEAAPPPAEEFPEPSPASSPSDQAESEAAEEGVQPENSESSEVKSTEKEEEPMEVETAEKSFQNQGTSNTATPLPQLPEPSPMTSYTMPNTNVTLETLLSTKVAVAQFSQSARSAASASISSGVTAVAIPMILEQLMALQQQQIHQLQLIEQIRSQVAMMNRQPLRPALNPVVAAPGVTGQASNQLQGFATSAAIQLTAVLPPAIMGQAAGAQPPAFDGSQHISRPTSGASTPNISSSGSSAPPESSAPCSSNAITSVTPVSVSNTTISASQPQNASTPPSIGHGSLTSISNLPNPLLPQTSSNSVIFPNPLVSIAATANALDPLSALMKHRKGKPPNVSVFEPKSSSEDPFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEYLDNVPTCSGIPYGMSLPPEKPVTTWLDSKPVLPTIPTSIGLQLPPTIPGVNSYGDSPSITPMSRSPQRPSPASSECTSLSPSLNTSESGVPVSAESPQPVQSSSSVTKAEPISLPPASTRLGDHSLSGQVSTASTSSIPTIVTDSSVSTSLPNPVLPAVSDQFKAKFPFGGLLDSMQTSETSKLQQLVENIDKKMTDPNQCVICHRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHFGVHRAKPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTPLPEGFQDAMDSELSYDEKNIDTLSNFDDDIDENSMEEDPELKDMASDSAKPLISYSGSCPSSPPSVISSIAALENQMKMIDSVMNCQQLTSLKSIENGSGESDHLSNDSSSAVGDLESQSAGSPAMSESSSSMQALSPVNSNSESFRSKSPGLSNQEEPQEIQLKTEKPDSPPPATENGGALDLTSTNPGRPVIKEEAPFSLLFLNRERGPSQSTPSLVTSTAPTMIKMEVNGHSKPISLGEVPSLPAGIQVPAAPQTVMSPGITPMLAPPPRRTPKQHNCQSCGKTFSSASALQIHERTHTGEKPFGCTICGRAFTTKGNLKVHMGTHMWNNAPARRGRRLSVENPMALLGGDALKFSEMFQKDLAARAMNVDPSFWNQYAAAITNGLAMKNNEISVIQNGGIPQLPVSLGGGAIPPLSNLTSGMDKARTGSSPPIVGLDKTSSETGASRPFTRFIEDNKEIGIN BB22E257BCAE8565 sal1-sal1-1 1 2 unspecified role unspecified role bait bait 2 unspecified role unspecified role prey prey physical association physical association Sal1 was HA tagged and FLAG tagged. FLAG taged Sal1 used as bait. Revisit when protien editor in place. sal2-sal2-1 1 3 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association Revisit when protien editor in place. Sal2 was HA tagged and FLAG tagged. FLAG taged Sal1 used as bait. sal1-sal2-1 1 2 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association Revisit when protien editor in place. Sal1 was HA tagged and Sal 2 was FLAG tagged. FLAG taged Sal1 used as bait. sal1-sal1-2 1 2 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait physical association physical association Revisit when protien editor in place. Sal1 was HA tagged and FLAG tagged. HA tagged Sal1 used as bait. Both Sal1 AA 1-290 and 1-806 could precipitated full length Sal1 however 1-218 failed to do so. sal2-sal2-2 1 3 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association Revisit when protien editor in place. Sal2 was HA tagged and FLAG tagged. HA tagged Sal2 used as bait. AA1-282 of Sal2 was precipitated by full length Sal2 however 1-230 could not do so. sal1-sal2-2 1 3 unspecified role unspecified role bait bait 2 unspecified role unspecified role prey prey physical association physical association Revisit when protien editor in place. HA taged Sal1 AA 1-209 used as bait and it preciitated AA 1-282 of FLAG tagged Sal2. Deletion analysis indicated Sal1 AA 1-240 and Sal2 AA 1-252 proteins were able to interact.