IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Histone methyltransferase activity of a Drosophila Polycomb group repressor complex. Histone methyltransferase activity of a Drosophila Polycomb group repressor complex. Cell (0092-8674) 2002 Muller J., Hart CM., Francis NJ., Vargas ML., Sengupta A., Wild B., Miller EL., O'Connor MB., Kingston RE., Simon JA. Chromatin - Epigenetic interactions resulting in chromatin modulation mueller@embl-heidelberg.de in vitro In vitro coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip predetermined predetermined participant predetermined Muller J., Hart CM., Francis NJ., Vargas ML., Sengupta A., Wild B., Miller EL., O'Connor MB., Kingston RE., Simon JA. Chromatin - Epigenetic interactions resulting in chromatin modulation mueller@embl-heidelberg.de Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Cell (0092-8674) 2002 Histone methyltransferase activity of a Drosophila Polycomb group repressor complex. Histone methyltransferase activity of a Drosophila Polycomb group repressor complex. Cell (0092-8674) 2002 Muller J., Hart CM., Francis NJ., Vargas ML., Sengupta A., Wild B., Miller EL., O'Connor MB., Kingston RE., Simon JA. Chromatin - Epigenetic interactions resulting in chromatin modulation mueller@embl-heidelberg.de drome Drosophila melanogaster (Fruit fly) Fruit fly coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Muller J., Hart CM., Francis NJ., Vargas ML., Sengupta A., Wild B., Miller EL., O'Connor MB., Kingston RE., Simon JA. Chromatin - Epigenetic interactions resulting in chromatin modulation mueller@embl-heidelberg.de Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Cell (0092-8674) 2002 Histone methyltransferase activity of a Drosophila Polycomb group repressor complex. Histone methyltransferase activity of a Drosophila Polycomb group repressor complex. Cell (0092-8674) 2002 Muller J., Hart CM., Francis NJ., Vargas ML., Sengupta A., Wild B., Miller EL., O'Connor MB., Kingston RE., Simon JA. Chromatin - Epigenetic interactions resulting in chromatin modulation mueller@embl-heidelberg.de drome Drosophila melanogaster (Fruit fly) Fruit fly affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom fingerprinting peptide massfingerprinting fingerprinting Muller J., Hart CM., Francis NJ., Vargas ML., Sengupta A., Wild B., Miller EL., O'Connor MB., Kingston RE., Simon JA. Chromatin - Epigenetic interactions resulting in chromatin modulation The detailed methods of mass spectrometry was not defined. mueller@embl-heidelberg.de Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Cell (0092-8674) 2002 ez_drome Histone-lysine N-methyltransferase E(z) KMT6 E(z) CG6502 Protein enhancer of zeste Lysine N-methyltransferase 6 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNSTKVPPEWKRRVKSEYIKIRQQKRYKRADEIKEAWIRNWDEHNHNVQDLYCESKVWQAKPYDPPHVDCVKRAEVTSYNGIPSGPQKVPICVINAVTPIPTMYTWAPTQQNFMVEDETVLHNIPYMGDEVLDKDGKFIEELIKNYDGKVHGDKDPSFMDDAIFVELVHALMRSYSKELEEAAPGTATAIKTETLAKSKQGEDDGVVDVDADGESPMKLEKTDSKGDLTEVEKKETEEPLETEDADVKPDVEEVKDKLPFPAPIIFQAISANFPDKGTAQELKEKYIELTEHQDPERPQECTPNIDGIKAESVSRERTMHSFHTLFCRRCFKYDCFLHRLQGHAGPNLQKRRYPELKPFAEPCSNSCYMLIDGMKEKLAADSKTPPIDSCNEASSEDSNDSNSQFSNKDFNHENSKDNGLTVNSAAVAEINSIMAGMMNITSTQCVWTGADQALYRVLHKVYLKNYCAIAHNMLTKTCRQVYEFAQKEDAEFSFEDLRQDFTPPRKKKKKQRLWSLHCRKIQLKKDSSSNHVYNYTPCDHPGHPCDMNCSCIQTQNFCEKFCNCSSDCQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCQACGADQFKLTKITCKNVCVQRGLHKHLLMAPSDIAGWGIFLKEGAQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSINPNCYAKVMMVTGDHRIGIFAKRAIQPGEELFFDYRYGPTEQLKFVGIEREMEIV 022360ED7772EB60 suz12_drome Polycomb protein Su(z)12 Su(z)12 CG8013 Suppressor 12 of zeste protein protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAPAKKREKDSNPDGSAANGIIGLTHGAPDASNAGSTVPPTAEGQVKLNGHQQEQELFLQAFEKPTQIYRYLRNRHETNPIFLNRTLSYMKERMSRNNKKRISFQVNSMLESITQKSEAVSQNYLHVIYDSLHEKLPARLDNESGEDLLQEQLLCEAGESVSVETTLYKITRSKRKDSTLDFQELLSKCSQIVYNPKDRVGEHATISIPLQTMRPMGEQHTLYKLLFRIKVLSPSTCNDENAETPPNKRSRPNEKMFGSELILYEKSSGFITEGEYEAMLQPLNSTSIKSFSPKKCTWETMPDSYIPLSLTYDVYQQSPMLKFHLTLSNEQLPEMISAPELQRYVQHLDAVAEMNYNNNNYNNNNNCSGLKNGSGGGNSTVCKTTPEHIQIVYNFMYSNNTRQQTEYTQELNCPWCGLDCLRLYALLKHLKLCHARFNFTYQPAGSGARIDVTINDAYDGSYAGSPYDLAGPSGSSFARTCGPVRRTSVTSLMVCRPRRQKTCLDEFLELDEDEISNQRSYITGHNRLYHHTETCLPVHPKELDIDSEGESDPLWLRQKTIQMIDEFSDVNEGEKELMKLWNLHVMRHGFVGDCQLPIACEMFLDAKGTEIVRKNLYRNFILHMCSLFDYGLIAAETVYKTVQKLQGLLSKYAAGQELMQRQREEQLKYWLDVGMHKKQEDPKTLKSPQKPAPPADQASTSSASTSGSGSGSSSMQPPKRMPAHLKRGSAASSPGVQSKGTENGTNGSNSSSSNSKNVAKKSADQPLSTLANTRERRSEYGQKRNVSGSRLAATPASKRKLSSKDNTVLNKRQRYSDGSPGTGIGNGHGGGSGSGANRNKSNNHSLPATSNNASSSSSNSKRAIARRRSTSERTKASGSTGGGAGGVRTRLSVPAKYERR 53BA0D83C49EC92F esc_drome Polycomb protein esc esc CG14941 Protein extra sex combs protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSSDKVKNGNEPEESEESCGDESASYTTNSTTSRSKSPSSSTRSKRRGRRSTKSKPKSRAAYKYDTHVKENHGANIFGVAFNTLLGKDEPQVFATAGSNRVTVYECPRQGGMQLLHCYADPDPDEVFYTCAWSYDLKTSSPLLAAAGYRGVIRVIDVEQNEAVGNYIGHGQAINELKFHPHKLQLLLSGSKDHAIRLWNIQSHVCIAILGGVEGHRDEVLSIDFNMRGDRIVSSGMDHSLKLWCLNTPEFHHKIELSNTFSQEKSTLPFPTVTKHFPDFSTRDIHRNYVDCVQWFGNFVLSKSCENAIVCWKPGQLHQSFEQVKPSDSSCTIIAEFEYDECEIWFVRFGFNPWQKVIALGNQQGKVYVWELDPSDPEGAHMTTLHNSRSVATVRQIAFSRDASVLVYVCDDATVWRWNRRQTTSI 511C305E5DE86727 caf1_drome Chromatin assembly factor 1 p55 subunit Caf1-55 CAF-1 Caf1 Nucleosome-remodeling factor 55 kDa subunit CG4236 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MVDRSDNAAESFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTKQDGKDYSVHRLILGTHTSDEQNHLLIASVQLPSEDAQFDGSHYDNEKGEFGGFGSVCGKIEIEIKINHEGEVNRARYMPQNACVIATKTPSSDVLVFDYTKHPSKPEPSGECQPDLRLRGHQKEGYGLSWNPNLNGYLLSASDDHTICLWDINATPKEHRVIDAKNIFTGHTAVVEDVAWHLLHESLFGSVADDQKLMIWDTRNNNTSKPSHTVDAHTAEVNCLSFNPYSEFILATGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLHVWDLSKIGEEQSTEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWIICSVSEDNIMQVWQMAENVYNDEEPEIPASELETNTA DIP protein Q24572 original sequence version: 142 DIP protein Q24572 original sequence version: 124 90C5FB959F1660D5 su_z_12-esc-1 2 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey association association ESC is FLAG tagged esc-caf1-1 1 6 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey association association The proteins were expressed in Sf9 cells from baculoviruses. The reconstituted complex had methyltransferase activity. esc-caf1-2 3 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey association association ESC is FLAG tagged