IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ UbcD4, a ubiquitin-conjugating enzyme in Drosophila melanogaster expressed in pole cells. UbcD4, a ubiquitin-conjugating enzyme in Drosophila melanogaster expressed in pole cells. Mol. Genet. Genomics (1617-4615) 2002 Canning M., Kirby R., Finnegan D. Yeast strain used is EGY48 (not in CABRI). Embryonic cDNA library is used as prey. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid full dna sequence full identification by DNA sequencing full dna sequence Canning M., Kirby R., Finnegan D. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Yeast strain used is EGY48 (not in CABRI). Embryonic cDNA library is used as prey. Mol. Genet. Genomics (1617-4615) 2002 UbcD4, a ubiquitin-conjugating enzyme in Drosophila melanogaster expressed in pole cells. UbcD4, a ubiquitin-conjugating enzyme in Drosophila melanogaster expressed in pole cells. Mol. Genet. Genomics (1617-4615) 2002 Canning M., Kirby R., Finnegan D. Yeast strain used is EGY48 (not in CABRI). Embryonic cDNA library is used as prey. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip predetermined predetermined participant predetermined Canning M., Kirby R., Finnegan D. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Yeast strain used is EGY48 (not in CABRI). Embryonic cDNA library is used as prey. Mol. Genet. Genomics (1617-4615) 2002 q9w196_drome HECT-type E3 ubiquitin transferase Dmel\CG3356 CG3356 Dmel_CG3356 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MFGFDGEYRRRPVQSLGGASHTCDRDTVIRKAALERQKRNELRQKENGAVVLQSYARSFIHRQRRKRAEREVFDIYLMGHKDGIVEDESLTFLLRRLNFFYSIREAKDSERLIEVCQQILRQPARLLQHSSPDSLWLLRLCKLLDTCLLQLSLSHTAQAIPLRMLETFTTVSSVQQYMKDEAVLFQYMERVFGFLIARNYFVRLRRLLDDKCPPLDGETLHAPSPLAEALLQLLLRPLEVAKRASAGGQMSSMSMAVCRNFTRDILATPHTDPLRYFVLPCFALNVDFPFDLLMRSLYDALESAGPAESDSTSSRRSFLFYGVETGHKTTRMDSIFSSFLLNSLMVLDRRQLATLHQSPLLVIYVRLIAEMMPNILQLPKSTLRGHANAPHRHRDGDDDSEESEDEDEELPAARTLDYDMEQTCRTSGELSVKERECLLESIAILNETERVDFIVQQLDPHIENTHLIYALCEICHNLMIYNKHAVFEYKLLYTLAFTPKFIRAVWFKLAAESTQLGFSAPLTLISKGVVPKHQGVDRTIPLLATFCMLFGRLLPTLHDVEFVENKLLLQVHSTINHVRLMPFSIAEIIQMSKTLKDISMGLVELAFPETRSNLANYRKVLGHTEADDKKLRHQKQIWANLLNVVVFVLNQIHTRDLRLGFCPEDHWTVTRLDLPLDRPTDLPLTHSSRLRGIRPFQPIRDFTREDFENGPPMSTKQIRSITILREIPFVVPFNKRVSILQSLVAASKMRVQGNMQAFLQGPSVLITVRRSHLYEDAYDKLRPDNEPDLRFKFRIQFVSSLGLDEAGIDGGGVFREFLSELIKTAFDPNRGFFMVTTDNKLYPNPNVADLFEDYEKHYYFIGRILGKSIYENLLVELPLAEFFLTKLAGKYSDVDIHQLASLDPELYRNLLYLKDYSGDVSELNLDFTVASSSLGQTQIVELKPQGQSIPVTNSNRIEYLQLIADYKLNVQIRRHCNAFRKGLSNVLPIEWLYMFSNKELQILISGAEIPIDLEDLKKHCEYGGEFSPEHPSIVTFWEVLEGFDDMQRRQLLKFVTSCSRPPLLGFKDLDPPFFIQNTGDMERLPTASTCTNLLKLPPFKTVEQMREKLLYAIQSGAGFELS 4320D750A67D81F5 ubcd4_drome Ubiquitin-conjugating enzyme E2-22 kDa Ubiquitin-protein ligase CG8284 Ubc4 E2 ubiquitin-conjugating enzyme 4 UbcD4 Ubiquitin carrier protein protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MANMAVSRIKREFKEVMRSEEIVQCSIKIELVNDSWTELRGEIAGPPDTPYEGGKFVLEIKVPETYPFNPPKVRFITRIWHPNISSVTGAICLDILKDNWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVAYQFKDKYDLFLLTAKHWTNAYAGGPHTFPDCDSKIQRLRDMGIDEHEARAVLSKENWNLEKATEGLFS 2875F9478632C630 psmd4_drome 26S proteasome non-ATPase regulatory subunit 4 Rpn10 26S proteasome regulatory subunit RPN10 26S proteasome regulatory subunit S5A 54 kDa subunit of mu particle Multiubiquitin chain-binding protein PROS-54 Pros54 p54 CG7619 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MVLESTMICFDNSDFQRNGDYFPTRLIVQRDGINLVCLTKLRSNPENNVGLMTLSNTVEVLATLTSDAGRIFSKMHLVQPKGEINLLTGIRIAHLVLKHRQGKNHKMRIVVFVGSPINHEEGDLVKQAKRLKKEKVNVDIVSFGDHGNNNEILTAFINALNGKDGTGSHLVSVPRGSVLSDALLSSPIIQGEDGMGGAGLGGNVFEFGVDPNEDPELALALRVSMEEQRQRQESEQRRANPDGAPPTGGDAGGGGGVSGSGPGNEESAGAENEANTEEAMLQRALALSTETPEDNLPDFANMTEEEQIAFAMQMSMQDAPDDSVTQQAKRPKTDEANAPMDVDEDYSEVIGDPAFLQSVLENLPGVDPQSEAVRDAVGSLNKDKDKKSDGKDSQKK 061418F6F7FE819F ubcd4-cg3356-1 1 4 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 3 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association Authors have been unable to demonstrate co-immunoprecipitation of UbcD4 and CG3356 proteins in S2 cells so the significance of this Y2H interaction is not clear. ubcd4-pros54-1 1 5 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 4 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association ubcd4-pros54-2 2 4 unspecified role unspecified role prey prey act5c promoter uncategorized protein modification uncategorized protein modification n-term range n-terminal range n-term range n-term range n-terminal range n-term range region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 5 unspecified role unspecified role bait bait c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range act5c promoter uncategorized protein modification uncategorized protein modification n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. physical association physical association