MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI. Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI. Cell (0092-8674) 2000 Hoffman GR., Nassar N., Cerione RA. no in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hoffman GR., Nassar N., Cerione RA. no Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Cell (0092-8674) 2000 gdir1_bovin Rho GDP-dissociation inhibitor 1 Rho-GDI alpha ARHGDIA protein protein bovin Bos taurus (Bovine) Bovine MAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVVVTRLTLVCSTAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGPRAEEYEFLTPMEEAPKGMLARGSYNIKSRFTDDDRTDHLSWEWNLTIKKEWKD 49CE7DEB05D271CA p60953-2 Cell division control protein 42 homolog CDC42 G25K GTP-binding protein Placental protein protein human Homo sapiens Human MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL DIP protein P60953-2 original sequence version: 152 DIP protein P60953 original sequence version: 98 DIP protein P60953 original sequence version: 148 DIP protein P60953 original sequence version: 142 DIP protein P60953-2 original sequence version: 135 DIP protein P60953 original sequence version: 99 DIP protein P60953-2 original sequence version: 139 DIP protein P60953 original sequence version: 144 DIP protein P60953 original sequence version: 163 DIP protein P60953 original sequence version: 158 DIP protein P60953-2 original sequence version: 147 DIP protein P60953 original sequence version: 123 51A437E22A4D8FFF cdc42-gdir-1 1 2 unspecified role unspecified role neutral component neutral component gspgisggggilgl GSPGISGGGGGILGL tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range asp45 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ser47 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain tyr51 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain asp42 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lys52 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain pro30 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ala31 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain asp185 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain asp184 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ser148 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain asp143 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain thr142 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain tyr144 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gln130 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role neutral component neutral component gly ser his tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range geranylgeranyl cys sgergercys S-geranylgeranyl-L-cysteine 2-amino-3-(3,7,11,15-tetramethyl-2,6,10,14-hexadecatetraenylthio)propanoic acid LIPID S-geranylgeranyl cysteine S-geranylgeranyl-L-cysteine (2R)-2-amino-3-([(2E,6E,10Z)-12-hydroxy-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]sulfanyl)propanoic acid Geranyl-geranyl GeranylGeranyl S-geranylgeranyl SGergerCys geranylgeranylc certain certain sequence position certain certain certain sequence position certain region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain thr35 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain thr35 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ala59 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain tyr64 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain tyr64 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain arg66 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain arg66 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain arg66 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his103 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his104 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lys184 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain arg186 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain arg186 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain arg186 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain arg186 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain arg186 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 7 25 26 8 27 9 10 28 11 29 12 30 13 31 32 14 15 33 16 34 35 17 18 36 39 19 20 40 direct interaction direct interaction working 25.7%, free 32.0% 2.60A The geranylgeranyl linked to cysteine 188 of Cdc42 inserts into a hydrophobic pocket within the immunoglobulin-like domain of GDI. N-terminal GST-tag of GDI is cleaved but some amino acids remains Arg186, Tyr175, Pro166, Thr132, Tyr110, Gln130, Trp194, Leu75, Phe102, Leu88, Ile112, Leu77, Leu196, Ile177, Ile198, Leu104, Ile169, Ile14, Leu11 of GDI form the hydrophobic pocket.