MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. J. Biol. Chem. (0021-9258) 2002 Lim R., Winteringham LN., Williams JH., McCulloch RK., Ingley E., Tiao JY., Lalonde JP., Tsai S., Tilbrook PA., Sun Y., Wu X., Morris SW., Klinken SP. Table 1 pklinken@cyllene.uwa.edu.au A pVP16 cDNA library made from mRNA prepared from the lymphohemopoietic progenitor cell line EML C.1 was screened. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Lim R., Winteringham LN., Williams JH., McCulloch RK., Ingley E., Tiao JY., Lalonde JP., Tsai S., Tilbrook PA., Sun Y., Wu X., Morris SW., Klinken SP. Peptide conjugated to Sepharose beads and exposed to Cos-1 lysate expressing recombinant protein Table 1 pklinken@cyllene.uwa.edu.au Annotation to indicate that this entry has been checked and accepted by a senior curator. A pVP16 cDNA library made from mRNA prepared from the lymphohemopoietic progenitor cell line EML C.1 was screened. J. Biol. Chem. (0021-9258) 2002 MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. J. Biol. Chem. (0021-9258) 2002 Lim R., Winteringham LN., Williams JH., McCulloch RK., Ingley E., Tiao JY., Lalonde JP., Tsai S., Tilbrook PA., Sun Y., Wu X., Morris SW., Klinken SP. Table 1 pklinken@cyllene.uwa.edu.au A pVP16 cDNA library made from mRNA prepared from the lymphohemopoietic progenitor cell line EML C.1 was screened. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lim R., Winteringham LN., Williams JH., McCulloch RK., Ingley E., Tiao JY., Lalonde JP., Tsai S., Tilbrook PA., Sun Y., Wu X., Morris SW., Klinken SP. Table 1 pklinken@cyllene.uwa.edu.au Annotation to indicate that this entry has been checked and accepted by a senior curator. A pVP16 cDNA library made from mRNA prepared from the lymphohemopoietic progenitor cell line EML C.1 was screened. J. Biol. Chem. (0021-9258) 2002 MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. J. Biol. Chem. (0021-9258) 2002 Lim R., Winteringham LN., Williams JH., McCulloch RK., Ingley E., Tiao JY., Lalonde JP., Tsai S., Tilbrook PA., Sun Y., Wu X., Morris SW., Klinken SP. Table 1 pklinken@cyllene.uwa.edu.au A pVP16 cDNA library made from mRNA prepared from the lymphohemopoietic progenitor cell line EML C.1 was screened. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lim R., Winteringham LN., Williams JH., McCulloch RK., Ingley E., Tiao JY., Lalonde JP., Tsai S., Tilbrook PA., Sun Y., Wu X., Morris SW., Klinken SP. Table 1 pklinken@cyllene.uwa.edu.au Annotation to indicate that this entry has been checked and accepted by a senior curator. A pVP16 cDNA library made from mRNA prepared from the lymphohemopoietic progenitor cell line EML C.1 was screened. J. Biol. Chem. (0021-9258) 2002 MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. J. Biol. Chem. (0021-9258) 2002 Lim R., Winteringham LN., Williams JH., McCulloch RK., Ingley E., Tiao JY., Lalonde JP., Tsai S., Tilbrook PA., Sun Y., Wu X., Morris SW., Klinken SP. Table 1 pklinken@cyllene.uwa.edu.au A pVP16 cDNA library made from mRNA prepared from the lymphohemopoietic progenitor cell line EML C.1 was screened. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lim R., Winteringham LN., Williams JH., McCulloch RK., Ingley E., Tiao JY., Lalonde JP., Tsai S., Tilbrook PA., Sun Y., Wu X., Morris SW., Klinken SP. Cos-7 cells transfected with Mlf1-expressing construct. Table 1 pklinken@cyllene.uwa.edu.au Annotation to indicate that this entry has been checked and accepted by a senior curator. A pVP16 cDNA library made from mRNA prepared from the lymphohemopoietic progenitor cell line EML C.1 was screened. J. Biol. Chem. (0021-9258) 2002 MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. J. Biol. Chem. (0021-9258) 2002 Lim R., Winteringham LN., Williams JH., McCulloch RK., Ingley E., Tiao JY., Lalonde JP., Tsai S., Tilbrook PA., Sun Y., Wu X., Morris SW., Klinken SP. Table 1 pklinken@cyllene.uwa.edu.au A pVP16 cDNA library made from mRNA prepared from the lymphohemopoietic progenitor cell line EML C.1 was screened. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lim R., Winteringham LN., Williams JH., McCulloch RK., Ingley E., Tiao JY., Lalonde JP., Tsai S., Tilbrook PA., Sun Y., Wu X., Morris SW., Klinken SP. Cos-7 cells transfected with Mlf1 and/ or NRBP-expressing constructs. Table 1 pklinken@cyllene.uwa.edu.au Annotation to indicate that this entry has been checked and accepted by a senior curator. A pVP16 cDNA library made from mRNA prepared from the lymphohemopoietic progenitor cell line EML C.1 was screened. J. Biol. Chem. (0021-9258) 2002 MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. J. Biol. Chem. (0021-9258) 2002 Lim R., Winteringham LN., Williams JH., McCulloch RK., Ingley E., Tiao JY., Lalonde JP., Tsai S., Tilbrook PA., Sun Y., Wu X., Morris SW., Klinken SP. Table 1 pklinken@cyllene.uwa.edu.au A pVP16 cDNA library made from mRNA prepared from the lymphohemopoietic progenitor cell line EML C.1 was screened. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Lim R., Winteringham LN., Williams JH., McCulloch RK., Ingley E., Tiao JY., Lalonde JP., Tsai S., Tilbrook PA., Sun Y., Wu X., Morris SW., Klinken SP. Cos-7 cells cotransfected with Mlf1- and NRBP-expressing constructs. Table 1 pklinken@cyllene.uwa.edu.au Annotation to indicate that this entry has been checked and accepted by a senior curator. A pVP16 cDNA library made from mRNA prepared from the lymphohemopoietic progenitor cell line EML C.1 was screened. J. Biol. Chem. (0021-9258) 2002 1433z_cerae_protein 14-3-3 protein zeta/delta protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt mlf1_human Myeloid leukemia factor 1 Myelodysplasia-myeloid leukemia factor 1 MLF1 protein protein human Homo sapiens Human MFRMLNSSFEDDPFFSESILAHRENMRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK 08774217329F737A nrbp_mouse Nuclear receptor-binding protein MLF1 adapter molecule HLS7-interacting protein kinase Nrbp1 Madm Nrbp protein protein mouse Mus musculus Mouse MSEGESQTVVSSGSDPKVESSSLAPGLTSVSPPVTSTTSAASPEEEEESEDESEILEESPCGRWQKRREEVNQRNVPGIDSAYLAMDTEEGVEVVWNEVQFSERKNYKLQEEKVRAVFDNLIQLEHLNIVKFHKYWADVKENKARVIFITEYMSSGSLKQFLKKTKKNHKTMNEKAWKRWCTQILSALSYLHSCDPPIIHGNLTCDTIFIQHNGLIKIGSVAPDTINNHVKTCREEQKNLHFFAPEYGEVTNVTTAVDIYSFGMCALEMAVLEIQGNGESSYVPQEAISSAIQLLEDSLQREFIQKCLQSEPARRPTARELLFHPALFEVPSLKLLAAHCIVGHQHMIPENALEEITKNMDTSAVLAEIPAGPGREPVQTLYSQSPALELDKFLEDVRNGIYPLTAFGLPRPQQPQQEEVTSPVVPPSVKTPTPEPAEVETRKVVLMQCNIESVEEGVKHHLTLLLKLEDKLNRHLSCDLMPNESIPDLAAELVQLGFISEADQSRLTSLLEETLNKFNFTRNSTLNTATVTVSS 85DD5F6FC25C5AB7 nucl_mouse Nucleolin Protein C23 Ncl Nuc protein protein mouse Mus musculus Mouse MVKLAKAGKTHGEAKKMAPPPKEVEEDSEDEEMSEDEDDSSGEEEVVIPQKKGKKATTTPAKKVVVSQTKKAAVPTPAKKAAVTPGKKAVATPAKKNITPAKVIPTPGKKGAAQAKALVPTPGKKGAATPAKGAKNGKNAKKEDSDEDEDEEDEDDSDEDEDDEEEDEFEPPIVKGVKPAKAAPAAPASEDEEDDEDEDDEEDDDEEEEDDSEEEVMEITTAKGKKTPAKVVPMKAKSVAEEEDDEEEDEDDEDEDDEEEDDEDDDEEEEEEEPVKAAPGKRKKEMTKQKEAPEAKKQKVEGSEPTTPFNLFIGNLNPNKSVNELKFAISELFAKNDLAVVDVRTGTNRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGRDSKKVRAARTLLAKNLSFNITEDELKEVFEDAMEIRLVSQDGKSKGIAYIEFKSEADAEKNLEEKQGAEIDGRSVSLYYTGEKGQRQERTGKTSTWSGESKTLVLSNLSYSATKETLEEVFEKATFIKVPQNPHGKPKGYAFIEFASFEDAKEALNSCNKMEIEGRTIRLELQGSNSRSQPSKTLFVKGLSEDTTEETLKESFEGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDFKPQGKKTKFE EE2CE2ACDBF54CD4 cybp_mouse Calcyclin-binding protein Siah-interacting protein Cacybp Sip protein protein mouse Mus musculus Mouse MASVLEELQKDLEEVKVLLEKSTRKRLRDTLTSEKSKIETELKNKMQQKSQKKPELDNEKPAAVVAPLTTGYTVKISNYGWDQSDKFVKIYITLTGVHQVPTENVQVHFTERSFDLLVKNLNGKNYSMIVNNLLKPISVESSSKKVKTDTVIILCRKKAENTRWDYLTQVEKECKEKEKPSYDTEADPSEGLMNVLKKIYEDGDDDMKRTINKAWVESREKQAREDTEF 2B04B54D0054ACE0 mlf1_mouse Myeloid leukemia factor 1 Mlf1 Hls7 Myelodysplasia-myeloid leukemia factor 1 Hematopoietic lineage switch 7 protein protein mouse Mus musculus Mouse MFRMLSSSFEDDPFFADSFLAHRESMRNMMRSFSEPLGRDLLSISDGRGRTHNRRERDDGEDSLTHADVNPFQTMDRMMANMRSGIQELQRNFGQLSMDPNGHSFCSSSVMTYSKVGDEPPKVFQASTQTRRAPGGVKETRKAMRDSDSGLERMAVGHHIHDRGHVIRKSKNNKTGDEEVNQEFINMNESDAHAFDDEWQNEVLKYKSIGRSGNTGMRSVGHEHPGSRELKRREKIHRNSAIESGRRSNVFVDKLNVKGSPVKITKK 0DEF99C708CBE6B8 dyl2_mouse Dynein light chain 2, cytoplasmic Dynein light chain LC8-type 2 8 kDa dynein light chain b Dynll2 Dlc2 protein protein mouse Mus musculus Mouse MSDRKAVIKNADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 45364D32C0077F8E eif3g_mouse Eukaryotic translation initiation factor 3 subunit G Eif3s4 Eukaryotic translation initiation factor 3 subunit 4 eIF-3-delta Eukaryotic translation initiation factor 3 RNA-binding subunit eIF3 p44 eIF3 p42 Eif3g Eif3p42 protein protein mouse Mus musculus Mouse MPTGDFDSKPSWADQVEEEGEDDKCVTSELLKGIPLPTGDTSPEPELLPGDPLPPPKEVINGNIKTVTEYKIEEDGKKFKIVRTFRIETRKASKAVARRKNWKKFGNSEFDPPGPNVATTTVSDDVSMTFITSKEDLNCQEEEDPMNKLKGQKIVSCRICKGDHWTTRCPYKDTLGPMQKELAEQLGLSTGEKEKLPGELEPVQAAQSKTGKYVPPSLRDGASRRGESMQPNRRADDNATIRVTNLSEDTRETDLQELFRPFGSISRIYLAKDKTTGQSKGFAFISFHRREDAARAIAGVSGFGYDHLILNVEWAKPSTN 1B46670351E489AC 1433z_mouse 14-3-3 protein zeta/delta Ywhaz Protein kinase C inhibitor protein 1 SEZ-2 protein protein mouse Mus musculus Mouse MDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQPESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGEGGEN DIP protein P63101 original sequence version: 72 2164DF3793B45B7A mlf1-ywhaz-1 2 15 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 12 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association mlf1-dlc2-1 2 12 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 13 unspecified role unspecified role prey prey physical association physical association mlf1-eif3s4-1 2 14 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association mlf1-cacybp-1 2 12 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 11 unspecified role unspecified role prey prey physical association physical association mlf1-ncl-1 2 10 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association mlf1-ywhaz-2 4 7 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association Interaction inhibited by phosphorylated MLF-1 peptide MRQMIRSFSEPFGRDL mlf1-ywhaz-3 1 15 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ser34 (required) opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association mlf1-ywhaz-4 1 12 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ser34 (required) opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role prey prey physical association physical association mlf1-nrbp-1 2 12 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role prey prey physical association physical association NRBP is the protein identified as M44 in Table 1 mlf1-nrbp-2 5 9 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey physical association physical association mlf1-nrbp-3 6 9 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component physical association physical association mlf1-nrbp-4 3 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association mlf1-nrbp-5 3 12 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait physical association physical association mlf1-nrbp-6 3 8 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-767592]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 281-281, sequence length 268 [EBI-767592]175-281 9 unspecified role unspecified role bait bait physical association physical association nrbp-nrbp-1 5 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association nrbp-nrbp-2 3 9 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association