<?xml version='1.0' encoding='UTF-8'?>
<entrySet xmlns="http://psi.hupo.org/mi/mif300" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://psi.hupo.org/mi/mif300 https://raw.githubusercontent.com/HUPO-PSI/miXML/master/3.0/src/MIF300.xsd" level="3" version="0" minorVersion="0">
  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>UniProt</shortLabel>
        <fullName>UniProt</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0486" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-3989247" refType="identity" refTypeAc="MI:0356"/>
      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://www.uniprot.org</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Aip1p interacts with cofilin to disassemble actin filaments.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="10366597" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1993003" refType="identity" refTypeAc="MI:0356"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Aip1p interacts with cofilin to disassemble actin filaments.</attribute>
            <attribute name="journal" nameAc="MI:0885">J. Cell Biol. (0021-9525)</attribute>
            <attribute name="publication year" nameAc="MI:0886">1999</attribute>
            <attribute name="author-list" nameAc="MI:0636">Rodal AA., Tetreault JW., Lappalainen P., Drubin DG., Amberg DC.</attribute>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="10366597" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="4932">
            <names>
              <shortLabel>yeasx</shortLabel>
              <fullName>Saccharomyces cerevisiae (Baker's yeast)</fullName>
              <alias type="synonym" typeAc="MI:1041">Baker's yeast</alias>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>2 hybrid</shortLabel>
            <fullName>two hybrid</fullName>
            <alias type="go synonym" typeAc="MI:0303">2H</alias>
            <alias type="go synonym" typeAc="MI:0303">2h</alias>
            <alias type="go synonym" typeAc="MI:0303">Gal4 transcription regeneration</alias>
            <alias type="go synonym" typeAc="MI:0303">two-hybrid</alias>
            <alias type="go synonym" typeAc="MI:0303">yeast two hybrid</alias>
            <alias type="go synonym" typeAc="MI:0303">2-hybrid</alias>
            <alias type="go synonym" typeAc="MI:0303">classical two hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">classical two hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">2H</alias>
            <alias type="synonym" typeAc="MI:1041">two-hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">2-hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">Gal4 transcription regeneration</alias>
            <alias type="synonym" typeAc="MI:1041">2h</alias>
            <alias type="synonym" typeAc="MI:1041">yeast two hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">Y2H</alias>
            <alias type="synonym" typeAc="MI:1041">Y-2H</alias>
            <alias type="synonym" typeAc="MI:1041">2 hybrid</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0018" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-94" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="10967325" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="12634794" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="1946372" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Rodal AA., Tetreault JW., Lappalainen P., Drubin DG., Amberg DC.</attribute>
          <attribute name="accepted">Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01).</attribute>
          <attribute name="journal" nameAc="MI:0885">J. Cell Biol. (0021-9525)</attribute>
          <attribute name="publication year" nameAc="MI:0886">1999</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>aip1_yeast</shortLabel>
          <fullName>Actin-interacting protein 1</fullName>
          <alias type="gene name" typeAc="MI:0301">AIP1</alias>
          <alias type="orf name" typeAc="MI:0306">YM9582.17C</alias>
          <alias type="locus name" typeAc="MI:0305">YMR092C</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P46680" version="SP_68" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="D6VZR5" version="SP_100" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="ensemblfungi" id="YMR092C" version="SP_182" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-2406" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_013810.1"/>
          <secondaryRef db="sgd" dbAc="MI:0484" id="S000004698"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-SCE-114608"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-890N"/>
          <secondaryRef db="ensemblfungi" id="YMR092C" version="SP_182" refType="gene"/>
          <secondaryRef db="ensemblfungi" id="YMR092C_mRNA" version="SP_182" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0003779" version="SP_182"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005634" version="SP_182"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005737" version="SP_182"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005884" version="SP_182"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0030042" version="SP_182"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0030479" version="SP_182"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0030836" version="SP_182"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0030864" version="SP_182"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0032466" version="SP_182"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0043332" version="SP_182"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0051015" version="SP_182"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0051016" version="SP_182"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR001680" version="SP_182"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR015943" version="SP_182"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036322" version="SP_182"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1PGU" version="SP_182"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1PI6" version="SP_182"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="559292">
          <names>
            <shortLabel>yeast</shortLabel>
            <fullName>Saccharomyces cerevisiae</fullName>
            <alias type="synonym" typeAc="MI:1041">Baker's yeast</alias>
          </names>
        </organism>
        <sequence>MSSISLKEIIPPQPSTQRNFTTHLSYDPTTNAIAYPCGKSAFVRCLDDGDSKVPPVVQFTGHGSSVVTTVKFSPIKGSQYLCSGDESGKVIVWGWTFDKESNSVEVNVKSEFQVLAGPISDISWDFEGRRLCVVGEGRDNFGVFISWDSGNSLGEVSGHSQRINACHLKQSRPMRSMTVGDDGSVVFYQGPPFKFSASDRTHHKQGSFVRDVEFSPDSGEFVITVGSDRKISCFDGKSGEFLKYIEDDQEPVQGGIFALSWLDSQKFATVGADATIRVWDVTTSKCVQKWTLDKQQLGNQQVGVVATGNGRIISLSLDGTLNFYELGHDEVLKTISGHNKGITALTVNPLISGSYDGRIMEWSSSSMHQDHSNLIVSLDNSKAQEYSSISWDDTLKVNGITKHEFGSQPKVASANNDGFTAVLTNDDDLLILQSFTGDIIKSVRLNSPGSAVSLSQNYVAVGLEEGNTIQVFKLSDLEVSFDLKTPLRAKPSYISISPSETYIAAGDVMGKILLYDLQSREVKTSRWAFHTSKINAISWKPAEKGANEEEIEEDLVATGSLDTNIFIYSVKRPMKIIKALNAHKDGVNNLLWETPSTLVSSGADACIKRWNVVLE</sequence>
        <attributeList>
          <attribute name="crc64">B8340BF68DD08257</attribute>
          <attribute name="crc64">B8340BF68DD08257</attribute>
        </attributeList>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>cofi_yeast</shortLabel>
          <fullName>Cofilin</fullName>
          <alias type="gene name" typeAc="MI:0301">COF1</alias>
          <alias type="locus name" typeAc="MI:0305">YLL050C</alias>
          <alias type="orf name" typeAc="MI:0306">L0596</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Actin-depolymerizing factor 1</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q03048" version="SP_72" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q05307" version="SP_72" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q2XN65" version="SP_72" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="D6VXV8" version="SP_103" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="ensemblfungi" id="YLL050C" version="SP_189" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-4853" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_013050.1"/>
          <secondaryRef db="sgd" dbAc="MI:0484" id="S000003973"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-197N"/>
          <secondaryRef db="mint" dbAc="MI:0471" id="Q03048"/>
          <secondaryRef db="ensemblfungi" id="YLL050C" version="SP_189" refType="gene"/>
          <secondaryRef db="ensemblfungi" id="YLL050C_mRNA" version="SP_189" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005737" version="SP_189"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005886" version="SP_189"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006897" version="SP_189"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0007015" version="SP_189"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0015629" version="SP_189"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016363" version="SP_189"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0030042" version="SP_189"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0030479" version="SP_189"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0043001" version="SP_189"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0051014" version="SP_189"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0051015" version="SP_189"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR002108" version="SP_189"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR017904" version="SP_189"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR029006" version="SP_189"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1CFY" version="SP_189"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1COF" version="SP_189"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1QPV" version="SP_189"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="4KED" version="SP_189"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="4KEE" version="SP_189"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="4KEF" version="SP_189"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="559292">
          <names>
            <shortLabel>yeast</shortLabel>
            <fullName>Saccharomyces cerevisiae</fullName>
            <alias type="synonym" typeAc="MI:1041">Baker's yeast</alias>
          </names>
        </organism>
        <sequence>MSRSGVAVADESLTAFNDLKLGKKYKFILFGLNDAKTEIVVKETSTDPSYDAFLEKLPENDCLYAIYDFEYEINGNEGKRSKIVFFTWSPDTAPVRSKMVYASSKDALRRALNGVSTDVQGTDFSEVSYDSVLERVSRGAGSH</sequence>
        <attributeList>
          <attribute name="crc64">7A03747B0F21F22D</attribute>
          <attribute name="crc64">7A03747B0F21F22D</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4">
        <names>
          <shortLabel>cof1-aip1-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-79959" refType="identity" refTypeAc="MI:0356"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
          <participant id="6">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
      </interaction>
    </interactionList>
  </entry>
</entrySet>