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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>MINT</shortLabel>
        <fullName>MINT, Dpt of Biology, University of Rome Tor Vergata</fullName>
        <alias type="synonym" typeAc="MI:1041">MINT</alias>
      </names>
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      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it/mint</attribute>
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        <attribute name="definition">The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules.</attribute>
        <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>The interaction between rac1 and its guanine nucleotide dissociation inhibitor (GDI), monitored by a single fluorescent coumarin attached to GDI.</fullName>
        </names>
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          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">The interaction between rac1 and its guanine nucleotide dissociation inhibitor (GDI), monitored by a single fluorescent coumarin attached to GDI.</attribute>
            <attribute name="journal" nameAc="MI:0885">Biochemistry (0006-2960)</attribute>
            <attribute name="publication year" nameAc="MI:0886">1999</attribute>
            <attribute name="author-list" nameAc="MI:0636">Newcombe AR., Stockley RW., Hunter JL., Webb MR.</attribute>
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        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>x-ray diffraction</shortLabel>
            <fullName>x-ray crystallography</fullName>
            <alias type="go synonym" typeAc="MI:0303">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">x-ray diffraction</alias>
          </names>
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        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
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          <attribute name="author-list" nameAc="MI:0636">Newcombe AR., Stockley RW., Hunter JL., Webb MR.</attribute>
          <attribute name="uniprot-dr-export">no</attribute>
          <attribute name="journal" nameAc="MI:0885">Biochemistry (0006-2960)</attribute>
          <attribute name="publication year" nameAc="MI:0886">1999</attribute>
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    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>rac1_canlf</shortLabel>
          <fullName>Ras-related C3 botulinum toxin substrate 1</fullName>
          <alias type="gene name" typeAc="MI:0301">RAC1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">p21-Rac1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Rac2</alias>
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          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001003274.1"/>
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        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
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        </interactorType>
        <organism ncbiTaxId="9615">
          <names>
            <shortLabel>canlf</shortLabel>
            <fullName>Canis familiaris (dog)</fullName>
            <alias type="synonym" typeAc="MI:1041">Canis lupus familiaris</alias>
            <alias type="synonym" typeAc="MI:1041">Dog</alias>
            <alias type="synonym" typeAc="MI:1041">Canis familiaris</alias>
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        </organism>
        <sequence>MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL</sequence>
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          <attribute name="remark-internal">DIP protein P62999 original sequence version: 66</attribute>
          <attribute name="comment" nameAc="MI:0612">mint</attribute>
          <attribute name="function" nameAc="MI:0613">Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles</attribute>
          <attribute name="crc64">ACEDF83A45E5EA67</attribute>
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      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>gdir1_human</shortLabel>
          <fullName>Rho GDP-dissociation inhibitor 1</fullName>
          <alias type="gene name synonym" typeAc="MI:0302">Rho-GDI alpha</alias>
          <alias type="gene name" typeAc="MI:0301">ARHGDIA</alias>
          <alias type="gene name synonym" typeAc="MI:0302">GDIA1</alias>
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          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2JHW" version="SP_202"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2JHX" version="SP_202"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2JHY" version="SP_202"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2JHZ" version="SP_202"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2JI0" version="SP_202"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2N80" version="SP_202"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-193634" version="SP_202"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-209563" version="SP_202"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-8980692" version="SP_202"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-9013106" version="SP_202"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-9013148" version="SP_202"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-9013149" version="SP_202"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-9013404" version="SP_202"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-9013407" version="SP_202"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-9013408" version="SP_202"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005634" version="SP_202"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001172006.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001172007.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_004300.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001288169.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001288170.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001288171.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001288172.1"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
          </names>
        </organism>
        <sequence>MAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVVVTGLTLVCSSAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGPRAEEYEFLTPVEEAPKGMLARGSYSIKSRFTDDDKTDHLSWEWNLTIKKDWKD</sequence>
        <attributeList>
          <attribute name="crc64">59CB6F42E3B3BCCA</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4">
        <names>
          <shortLabel>arhgdia-rac1-2</shortLabel>
        </names>
        <xref>
          <primaryRef db="mint" dbAc="MI:0471" id="MINT-15393" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-7002683" refType="identity" refTypeAc="MI:0356"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <xref>
              <primaryRef db="mint" dbAc="MI:0471" id="MINT-368289" refType="identity" refTypeAc="MI:0356"/>
            </xref>
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <stoichiometry value="1"/>
            <attributeList>
              <attribute name="comment" nameAc="MI:0612">Stoichiometry: 1.0</attribute>
            </attributeList>
          </participant>
          <participant id="6">
            <xref>
              <primaryRef db="mint" dbAc="MI:0471" id="MINT-368293" refType="identity" refTypeAc="MI:0356"/>
            </xref>
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <stoichiometry value="1"/>
            <attributeList>
              <attribute name="comment" nameAc="MI:0612">Stoichiometry: 1.0</attribute>
            </attributeList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="comment" nameAc="MI:0612">homomint</attribute>
          <attribute name="comment" nameAc="MI:0612">mint</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>