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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>MINT</shortLabel>
        <fullName>MINT, Dpt of Biology, University of Rome Tor Vergata</fullName>
        <alias type="synonym" typeAc="MI:1041">MINT</alias>
      </names>
      <bibref>
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      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it/mint</attribute>
        <attribute name="url" nameAc="MI:0614">url&amp;gt;http://mint.bio.uniroma2.it/mint/</attribute>
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        <attribute name="definition">The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules.</attribute>
        <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Recruitment, activation and retention of caspases-9 and -3 by Apaf-1 apoptosome and associated XIAP complexes.</fullName>
        </names>
        <bibref>
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          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Recruitment, activation and retention of caspases-9 and -3 by Apaf-1 apoptosome and associated XIAP complexes.</attribute>
            <attribute name="journal" nameAc="MI:0885">EMBO J. (0261-4189)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2001</attribute>
            <attribute name="author-list" nameAc="MI:0636">Bratton SB., Walker G., Srinivasula SM., Sun XM., Butterworth M., Alnemri ES., Cohen GM.</attribute>
            <attribute name="uniprot-dr-export">no</attribute>
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        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>experimental interac</shortLabel>
            <fullName>experimental interaction detection</fullName>
            <alias type="synonym" typeAc="MI:1041">experimental interac</alias>
          </names>
          <xref>
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        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
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            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
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        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Bratton SB., Walker G., Srinivasula SM., Sun XM., Butterworth M., Alnemri ES., Cohen GM.</attribute>
          <attribute name="uniprot-dr-export">no</attribute>
          <attribute name="journal" nameAc="MI:0885">EMBO J. (0261-4189)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2001</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>casp9_human</shortLabel>
          <fullName>Caspase-9</fullName>
          <alias type="gene name synonym" typeAc="MI:0302">ICE-like apoptotic protease 6</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Apoptotic protease Mch-6</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Apoptotic protease-activating factor 3</alias>
          <alias type="gene name" typeAc="MI:0301">CASP9</alias>
          <alias type="gene name synonym" typeAc="MI:0302">MCH6</alias>
        </names>
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        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
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        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
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        </organism>
        <sequence>MDEADRRLLRRCRLRLVEELQVDQLWDALLSRELFRPHMIEDIQRAGSGSRRDQARQLIIDLETRGSQALPLFISCLEDTGQDMLASFLRTNRQAAKLSKPTLENLTPVVLRPEIRKPEVLRPETPRPVDIGSGGFGDVGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKTS</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein P55211 original sequence version: 174</attribute>
          <attribute name="remark-internal">DIP protein P55211 original sequence version: 167</attribute>
          <attribute name="remark-internal">DIP protein P55211 original sequence version: 124</attribute>
          <attribute name="remark-internal">DIP protein P55211 original sequence version: 125</attribute>
          <attribute name="remark-internal">DIP protein P55211 original sequence version: 123</attribute>
          <attribute name="remark-internal">DIP protein P55211 original sequence version: 153</attribute>
          <attribute name="crc64">78E0180DF2A3BDD2</attribute>
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      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>casp3_human</shortLabel>
          <fullName>Caspase-3</fullName>
          <alias type="gene name synonym" typeAc="MI:0302">Apopain</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Cysteine protease CPP32</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Protein Yama</alias>
          <alias type="gene name synonym" typeAc="MI:0302">SREBP cleavage activity 1</alias>
          <alias type="gene name" typeAc="MI:0301">CASP3</alias>
          <alias type="gene name synonym" typeAc="MI:0302">CPP32</alias>
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          <secondaryRef db="refseq" dbAc="MI:0481" id="XP_011530603.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_004337.2"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_116786.1"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-268N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
          </names>
        </organism>
        <sequence>MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESFSFDATFHAKKQIPCIVSMLTKELYFYH</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein P42574 original sequence version: 108</attribute>
          <attribute name="remark-internal">DIP protein P42574 original sequence version: 171</attribute>
          <attribute name="remark-internal">DIP protein P42574 original sequence version: 111</attribute>
          <attribute name="remark-internal">DIP protein P42574 original sequence version: 105</attribute>
          <attribute name="remark-internal">DIP protein P42574 original sequence version: 131</attribute>
          <attribute name="remark-internal">DIP protein P42574 original sequence version: 132</attribute>
          <attribute name="remark-internal">DIP protein P42574 original sequence version: 134</attribute>
          <attribute name="remark-internal">DIP protein P42574 original sequence version: 162</attribute>
          <attribute name="remark-internal">DIP protein P42574 original sequence version: 189</attribute>
          <attribute name="remark-internal">DIP protein P42574 original sequence version: 167</attribute>
          <attribute name="crc64">2F35CD3BCF7FF64A</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4">
        <names>
          <shortLabel>casp3-casp9-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="mint" dbAc="MI:0471" id="MINT-18815" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-7781714" refType="identity" refTypeAc="MI:0356"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <xref>
              <primaryRef db="mint" dbAc="MI:0471" id="MINT-468832" refType="identity" refTypeAc="MI:0356"/>
            </xref>
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <attributeList>
              <attribute name="comment" nameAc="MI:0612">Stoichiometry: 1.0</attribute>
            </attributeList>
          </participant>
          <participant id="6">
            <xref>
              <primaryRef db="mint" dbAc="MI:0471" id="MINT-468836" refType="identity" refTypeAc="MI:0356"/>
            </xref>
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <attributeList>
              <attribute name="comment" nameAc="MI:0612">Stoichiometry: 1.0</attribute>
            </attributeList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>association</shortLabel>
            <fullName>association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0914" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813191" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="comment" nameAc="MI:0612">Method: Gel filtration of lysates depleted of the endogenous level protein and then replaced with different forms of the recombinant protein.Western blot analysis.</attribute>
          <attribute name="comment" nameAc="MI:0612">homomint</attribute>
          <attribute name="comment" nameAc="MI:0612">mint</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>