UniProt UniProt http://www.uniprot.org Interactions between HIV Rev and nuclear import and export factors: the Rev nuclear localisation signal mediates specific binding to human importin-beta. Interactions between HIV Rev and nuclear import and export factors: the Rev nuclear localisation signal mediates specific binding to human importin-beta. J. Mol. Biol. (0022-2836) 1997 imex curation Henderson B.R., Percipalle P. Virus - Publications including interactions involving viral proteins Origin of HIB-1 Rev according Zemmel et al, 1996, PubMed 8637008. Purification of importin alpha and beta according Percipalle et al, 1997, PubMed 9102465. n/a 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight silver stain molecular weight estimation by silver staining weight silver stain Henderson B.R., Percipalle P. Virus - Publications including interactions involving viral proteins Origin of HIB-1 Rev according Zemmel et al, 1996, PubMed 8637008. Purification of importin alpha and beta according Percipalle et al, 1997, PubMed 9102465. n/a Accepted 2015-OCT-13 AT 09:54 BST AT 09:54 BST by ORCHARD J. Mol. Biol. (0022-2836) 1997 IMEx imex curation EBI-1129657 correct like this? imp-a as another prey acting as inhibitor? bit strange the exp/biol role relationship; do not agree to add imp-a in a separate interaction as it is not shown on the gel (fig 7a what is different to fig 6). only added imp-a as a antagonist Interactions between HIV Rev and nuclear import and export factors: the Rev nuclear localisation signal mediates specific binding to human importin-beta. Interactions between HIV Rev and nuclear import and export factors: the Rev nuclear localisation signal mediates specific binding to human importin-beta. J. Mol. Biol. (0022-2836) 1997 imex curation Henderson B.R., Percipalle P. Virus - Publications including interactions involving viral proteins Origin of HIB-1 Rev according Zemmel et al, 1996, PubMed 8637008. Purification of importin alpha and beta according Percipalle et al, 1997, PubMed 9102465. n/a 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom weight silver stain molecular weight estimation by silver staining weight silver stain Henderson B.R., Percipalle P. Virus - Publications including interactions involving viral proteins Origin of HIB-1 Rev according Zemmel et al, 1996, PubMed 8637008. Purification of importin alpha and beta according Percipalle et al, 1997, PubMed 9102465. n/a Accepted 2015-OCT-13 AT 09:54 BST AT 09:54 BST by ORCHARD J. Mol. Biol. (0022-2836) 1997 imex curation EBI-1129657 correct like this? imp-a as another prey acting as inhibitor? bit strange the exp/biol role relationship; do not agree to add imp-a in a separate interaction as it is not shown on the gel (fig 7a what is different to fig 6). only added imp-a as a antagonist Interactions between HIV Rev and nuclear import and export factors: the Rev nuclear localisation signal mediates specific binding to human importin-beta. Interactions between HIV Rev and nuclear import and export factors: the Rev nuclear localisation signal mediates specific binding to human importin-beta. J. Mol. Biol. (0022-2836) 1997 imex curation Henderson B.R., Percipalle P. Virus - Publications including interactions involving viral proteins Origin of HIB-1 Rev according Zemmel et al, 1996, PubMed 8637008. Purification of importin alpha and beta according Percipalle et al, 1997, PubMed 9102465. n/a 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Henderson B.R., Percipalle P. Virus - Publications including interactions involving viral proteins Mixed 35S-labelled Rev and unlabelled Rev (to provide excess of Rev over importin) have been used in a buffer containing 35S-labelled importin-alpha Origin of HIB-1 Rev according Zemmel et al, 1996, PubMed 8637008. Purification of importin alpha and beta according Percipalle et al, 1997, PubMed 9102465. n/a Accepted 2015-OCT-13 AT 09:54 BST AT 09:54 BST by ORCHARD J. Mol. Biol. (0022-2836) 1997 IMEx imex curation EBI-1129657 correct like this? imp-a as another prey acting as inhibitor? bit strange the exp/biol role relationship; do not agree to add imp-a in a separate interaction as it is not shown on the gel (fig 7a what is different to fig 6). only added imp-a as a antagonist rev_hv1br Protein Rev rev ART/TRS Anti-repression transactivator Regulator of expression of viral proteins protein protein hv1br Human immunodeficiency virus type 1 group M subtype B (isolate BRU/LAI) HIV-1 MAGRSGDSDEDLLKAVRLIKFLYQSNPPPNPEGTRQARRNRRRRWRERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILVESPTVLESGTKE 30DA320AF302FBCD ima1_human Importin subunit alpha-1 KPNA2 RCH1 SRP1 Karyopherin subunit alpha-2 SRP1-alpha RAG cohort protein 1 protein protein human Homo sapiens Human MSTNENANTPAARLHRFKNKGKDSTEMRRRRIEVNVELRKAKKDDQMLKRRNVSSFPDDATSPLQENRNNQGTVNWSVDDIVKGINSSNVENQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGRTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSVFRDLVIKYGAVDPLLALLAVPDMSSLACGYLRNLTWTLSNLCRNKNPAPPIDAVEQILPTLVRLLHHDDPEVLADTCWAISYLTDGPNERIGMVVKTGVVPQLVKLLGASELPIVTPALRAIGNIVTGTDEQTQVVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVSVLSKADFKTQKEAVWAVTNYTSGGTVEQIVYLVHCGIIEPLMNLLTAKDTKIILVILDAISNIFQAAEKLGETEKLSIMIEECGGLDKIEALQNHENESVYKASLSLIEKYFSVEEEEDQNVVPETTSEGYTFQVQDGAPGTFNF DIP protein P52292 original sequence version: 180 DIP protein P52292 original sequence version: 128 B0F94A0475B80EED imb1_human Importin subunit beta-1 Karyopherin subunit beta-1 Nuclear factor p97 Importin-90 KPNB1 NTF97 Pore targeting complex 97 kDa subunit protein protein human Homo sapiens Human MELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAAGLQIKNSLTSKDPDIKAQYQQRWLAIDANARREVKNYVLQTLGTETYRPSSASQCVAGIACAEIPVNQWPELIPQLVANVTNPNSTEHMKESTLEAIGYICQDIDPEQLQDKSNEILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKESERHFIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPEPSQLKPLVIQAMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQSNIIPFCDEVMQLLLENLGNENVHRSVKPQILSVFGDIALAIGGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDYLNELRESCLEAYTGIVQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDGVVACAAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKAKTLATWATKELRKLKNQA F3BB8B73E7E51639 rev-kpna2-2 3 5 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 4a rev-kpna2-1 1 5 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) a Rev arg38asp_arg39leu mutant also interacted physical association physical association 4c Not changed by HIV-1 Rev mutant arg_arg38-39asp_leu rev-kpnb1-1 1 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined true P04620:p.Arg38_Arg39delinsAspLeu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain RR DL Wed May 02 17:37:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg38-39asp_leu' Tue Jun 30 20:00:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg38_Arg39delinsAspLeu' ecoli Escherichia coli (strain K12) a Rev arg38asp_arg39leu mutant also interacted 6 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) physical association physical association 5 and 7a and b tRNA (in a twofold excess) KPNA2/importin-alpha IBB domain (InterPro; IPR00265) RAN (GTP-bound) kpnb1-rev-1 2 5 competitor competitor competitor prey prey ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined true ecoli Escherichia coli (strain K12) a Rev arg38asp_arg39leu mutant also interacted 6 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) association association 6 tRNA (in a twofold excess) KPNA2/importin-alpha IBB domain (InterPro; IPR00265) RAN (GTP-bound) kpnb1-rev-2 2 4 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined true ecoli Escherichia coli (strain K12) a Rev arg38asp_arg39leu mutant also interacted 6 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) physical association physical association 6 tRNA (in a twofold excess) KPNA2/importin-alpha IBB domain (InterPro; IPR00265) RAN (GTP-bound)