IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells. Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells. Proceedings of the National Academy of Sciences of the United States of America 2021 imex curation Chen J., Fan J., Chen Z., Zhang M., Peng H., Liu J., Ding L., Liu M., Zhao C., Zhao P., Zhang S., Zhang X., Xu J. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus email sent 22/12 requesting more info about "S606-APEX2-FLAG" + expression system/purity. The authors have stated that:- 1]"S606" is a truncated Spike protein (position 1-606 amino acid) of SARS-CoV-2 (UniProtKB - P0DTC2)，but not a mutation at residue 606. 2] Proximity labelling: Cells were treated with S606-APEX2-FLAG, as in the methods "APEX2" section; A fusion expression system was constructed: pSV1.0-S606-APEX-FLAG, was transfected into HEK293T cells. This fusion protein was purified from the cell pellet using FLAG agarose beads. pnzhao@163.com, zhangshuye@ shphc.org.cn, zhangxiaoyan@shphc.org.cn, xujianqing@shphc.org.cn. the email address xujianqing@shphc.org.cn is n.b.g. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chen J., Fan J., Chen Z., Zhang M., Peng H., Liu J., Ding L., Liu M., Zhao C., Zhao P., Zhang S., Zhang X., Xu J. Virus - Publications including interactions involving viral proteins email sent 22/12 requesting more info about "S606-APEX2-FLAG" + expression system/purity. The authors have stated that:- 1]"S606" is a truncated Spike protein (position 1-606 amino acid) of SARS-CoV-2 (UniProtKB - P0DTC2)，but not a mutation at residue 606. 2] Proximity labelling: Cells were treated with S606-APEX2-FLAG, as in the methods "APEX2" section; A fusion expression system was constructed: pSV1.0-S606-APEX-FLAG, was transfected into HEK293T cells. This fusion protein was purified from the cell pellet using FLAG agarose beads. pnzhao@163.com, zhangshuye@ shphc.org.cn, zhangxiaoyan@shphc.org.cn, xujianqing@shphc.org.cn. the email address xujianqing@shphc.org.cn is n.b.g. Accepted 2022-JAN-10 AT 07:50 GMT AT 07:50 GMT by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation imex curation Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells. Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells. Proceedings of the National Academy of Sciences of the United States of America 2021 imex curation Chen J., Fan J., Chen Z., Zhang M., Peng H., Liu J., Ding L., Liu M., Zhao C., Zhao P., Zhang S., Zhang X., Xu J. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus email sent 22/12 requesting more info about "S606-APEX2-FLAG" + expression system/purity. The authors have stated that:- 1]"S606" is a truncated Spike protein (position 1-606 amino acid) of SARS-CoV-2 (UniProtKB - P0DTC2)，but not a mutation at residue 606. 2] Proximity labelling: Cells were treated with S606-APEX2-FLAG, as in the methods "APEX2" section; A fusion expression system was constructed: pSV1.0-S606-APEX-FLAG, was transfected into HEK293T cells. This fusion protein was purified from the cell pellet using FLAG agarose beads. pnzhao@163.com, zhangshuye@ shphc.org.cn, zhangxiaoyan@shphc.org.cn, xujianqing@shphc.org.cn. the email address xujianqing@shphc.org.cn is n.b.g. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Chen J., Fan J., Chen Z., Zhang M., Peng H., Liu J., Ding L., Liu M., Zhao C., Zhao P., Zhang S., Zhang X., Xu J. Virus - Publications including interactions involving viral proteins email sent 22/12 requesting more info about "S606-APEX2-FLAG" + expression system/purity. The authors have stated that:- 1]"S606" is a truncated Spike protein (position 1-606 amino acid) of SARS-CoV-2 (UniProtKB - P0DTC2)，but not a mutation at residue 606. 2] Proximity labelling: Cells were treated with S606-APEX2-FLAG, as in the methods "APEX2" section; A fusion expression system was constructed: pSV1.0-S606-APEX-FLAG, was transfected into HEK293T cells. This fusion protein was purified from the cell pellet using FLAG agarose beads. pnzhao@163.com, zhangshuye@ shphc.org.cn, zhangxiaoyan@shphc.org.cn, xujianqing@shphc.org.cn. the email address xujianqing@shphc.org.cn is n.b.g. Accepted 2022-JAN-10 AT 07:50 GMT AT 07:50 GMT by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation imex curation Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells. Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells. Proceedings of the National Academy of Sciences of the United States of America 2021 imex curation Chen J., Fan J., Chen Z., Zhang M., Peng H., Liu J., Ding L., Liu M., Zhao C., Zhao P., Zhang S., Zhang X., Xu J. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus email sent 22/12 requesting more info about "S606-APEX2-FLAG" + expression system/purity. The authors have stated that:- 1]"S606" is a truncated Spike protein (position 1-606 amino acid) of SARS-CoV-2 (UniProtKB - P0DTC2)，but not a mutation at residue 606. 2] Proximity labelling: Cells were treated with S606-APEX2-FLAG, as in the methods "APEX2" section; A fusion expression system was constructed: pSV1.0-S606-APEX-FLAG, was transfected into HEK293T cells. This fusion protein was purified from the cell pellet using FLAG agarose beads. pnzhao@163.com, zhangshuye@ shphc.org.cn, zhangxiaoyan@shphc.org.cn, xujianqing@shphc.org.cn. the email address xujianqing@shphc.org.cn is n.b.g. Only protein-protein interactions imex curation in vitro In vitro proximity labelling technology proximity labelling technology western blot western blot Immuno blot Immuno blot Chen J., Fan J., Chen Z., Zhang M., Peng H., Liu J., Ding L., Liu M., Zhao C., Zhao P., Zhang S., Zhang X., Xu J. Virus - Publications including interactions involving viral proteins email sent 22/12 requesting more info about "S606-APEX2-FLAG" + expression system/purity. A target protein is tagged with "APEX2" -a mutated soybean ascorbate peroxidase (APX). APEX catalyzes the oxidation of added biotin-phenol (plus hydrogen peroxide) to generate a very short-lived biotin-phenoxyl radical which biotinylates proteins within a range of ∼20 nm of the APEX2-tagged protein. The authors have stated that:- 1]"S606" is a truncated Spike protein (position 1-606 amino acid) of SARS-CoV-2 (UniProtKB - P0DTC2)，but not a mutation at residue 606. 2] Proximity labelling: Cells were treated with S606-APEX2-FLAG, as in the methods "APEX2" section; A fusion expression system was constructed: pSV1.0-S606-APEX-FLAG, was transfected into HEK293T cells. This fusion protein was purified from the cell pellet using FLAG agarose beads. pnzhao@163.com, zhangshuye@ shphc.org.cn, zhangxiaoyan@shphc.org.cn, xujianqing@shphc.org.cn. the email address xujianqing@shphc.org.cn is n.b.g. Accepted 2022-JAN-10 AT 07:50 GMT AT 07:50 GMT by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation imex curation Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells. Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells. Proceedings of the National Academy of Sciences of the United States of America 2021 imex curation Chen J., Fan J., Chen Z., Zhang M., Peng H., Liu J., Ding L., Liu M., Zhao C., Zhao P., Zhang S., Zhang X., Xu J. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus email sent 22/12 requesting more info about "S606-APEX2-FLAG" + expression system/purity. The authors have stated that:- 1]"S606" is a truncated Spike protein (position 1-606 amino acid) of SARS-CoV-2 (UniProtKB - P0DTC2)，but not a mutation at residue 606. 2] Proximity labelling: Cells were treated with S606-APEX2-FLAG, as in the methods "APEX2" section; A fusion expression system was constructed: pSV1.0-S606-APEX-FLAG, was transfected into HEK293T cells. This fusion protein was purified from the cell pellet using FLAG agarose beads. pnzhao@163.com, zhangshuye@ shphc.org.cn, zhangxiaoyan@shphc.org.cn, xujianqing@shphc.org.cn. the email address xujianqing@shphc.org.cn is n.b.g. Only protein-protein interactions imex curation human-calu_3 Homo sapiens lung adenocarcinoma calu_3 male caucasian lung adenocarcinoma fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Chen J., Fan J., Chen Z., Zhang M., Peng H., Liu J., Ding L., Liu M., Zhao C., Zhao P., Zhang S., Zhang X., Xu J. Virus - Publications including interactions involving viral proteins email sent 22/12 requesting more info about "S606-APEX2-FLAG" + expression system/purity. The authors have stated that:- 1]"S606" is a truncated Spike protein (position 1-606 amino acid) of SARS-CoV-2 (UniProtKB - P0DTC2)，but not a mutation at residue 606. 2] Proximity labelling: Cells were treated with S606-APEX2-FLAG, as in the methods "APEX2" section; A fusion expression system was constructed: pSV1.0-S606-APEX-FLAG, was transfected into HEK293T cells. This fusion protein was purified from the cell pellet using FLAG agarose beads. pnzhao@163.com, zhangshuye@ shphc.org.cn, zhangxiaoyan@shphc.org.cn, xujianqing@shphc.org.cn. the email address xujianqing@shphc.org.cn is n.b.g. Accepted 2022-JAN-10 AT 07:50 GMT AT 07:50 GMT by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation imex curation Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells. Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells. Proceedings of the National Academy of Sciences of the United States of America 2021 imex curation Chen J., Fan J., Chen Z., Zhang M., Peng H., Liu J., Ding L., Liu M., Zhao C., Zhao P., Zhang S., Zhang X., Xu J. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus email sent 22/12 requesting more info about "S606-APEX2-FLAG" + expression system/purity. The authors have stated that:- 1]"S606" is a truncated Spike protein (position 1-606 amino acid) of SARS-CoV-2 (UniProtKB - P0DTC2)，but not a mutation at residue 606. 2] Proximity labelling: Cells were treated with S606-APEX2-FLAG, as in the methods "APEX2" section; A fusion expression system was constructed: pSV1.0-S606-APEX-FLAG, was transfected into HEK293T cells. This fusion protein was purified from the cell pellet using FLAG agarose beads. pnzhao@163.com, zhangshuye@ shphc.org.cn, zhangxiaoyan@shphc.org.cn, xujianqing@shphc.org.cn. the email address xujianqing@shphc.org.cn is n.b.g. Only protein-protein interactions imex curation in vitro In vitro proximity labelling technology proximity labelling technology sequence tag sequence tag identification sequence tag Chen J., Fan J., Chen Z., Zhang M., Peng H., Liu J., Ding L., Liu M., Zhao C., Zhao P., Zhang S., Zhang X., Xu J. Virus - Publications including interactions involving viral proteins email sent 22/12 requesting more info about "S606-APEX2-FLAG" + expression system/purity. A target protein is tagged with "APEX2" -a mutated soybean ascorbate peroxidase (APX). APEX catalyzes the oxidation of added biotin-phenol (plus hydrogen peroxide) to generate a very short-lived biotin-phenoxyl radical which biotinylates proteins within a range of ∼20 nm of the APEX2-tagged protein. The authors have stated that:- 1]"S606" is a truncated Spike protein (position 1-606 amino acid) of SARS-CoV-2 (UniProtKB - P0DTC2)，but not a mutation at residue 606. 2] Proximity labelling: Cells were treated with S606-APEX2-FLAG, as in the methods "APEX2" section; A fusion expression system was constructed: pSV1.0-S606-APEX-FLAG, was transfected into HEK293T cells. This fusion protein was purified from the cell pellet using FLAG agarose beads. pnzhao@163.com, zhangshuye@ shphc.org.cn, zhangxiaoyan@shphc.org.cn, xujianqing@shphc.org.cn. the email address xujianqing@shphc.org.cn is n.b.g. Accepted 2022-JAN-10 AT 07:50 GMT AT 07:50 GMT by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation imex curation Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells. Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells. Proceedings of the National Academy of Sciences of the United States of America 2021 imex curation Chen J., Fan J., Chen Z., Zhang M., Peng H., Liu J., Ding L., Liu M., Zhao C., Zhao P., Zhang S., Zhang X., Xu J. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus email sent 22/12 requesting more info about "S606-APEX2-FLAG" + expression system/purity. The authors have stated that:- 1]"S606" is a truncated Spike protein (position 1-606 amino acid) of SARS-CoV-2 (UniProtKB - P0DTC2)，but not a mutation at residue 606. 2] Proximity labelling: Cells were treated with S606-APEX2-FLAG, as in the methods "APEX2" section; A fusion expression system was constructed: pSV1.0-S606-APEX-FLAG, was transfected into HEK293T cells. This fusion protein was purified from the cell pellet using FLAG agarose beads. pnzhao@163.com, zhangshuye@ shphc.org.cn, zhangxiaoyan@shphc.org.cn, xujianqing@shphc.org.cn. the email address xujianqing@shphc.org.cn is n.b.g. Only protein-protein interactions imex curation human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Chen J., Fan J., Chen Z., Zhang M., Peng H., Liu J., Ding L., Liu M., Zhao C., Zhao P., Zhang S., Zhang X., Xu J. Virus - Publications including interactions involving viral proteins email sent 22/12 requesting more info about "S606-APEX2-FLAG" + expression system/purity. The authors have stated that:- 1]"S606" is a truncated Spike protein (position 1-606 amino acid) of SARS-CoV-2 (UniProtKB - P0DTC2)，but not a mutation at residue 606. 2] Proximity labelling: Cells were treated with S606-APEX2-FLAG, as in the methods "APEX2" section; A fusion expression system was constructed: pSV1.0-S606-APEX-FLAG, was transfected into HEK293T cells. This fusion protein was purified from the cell pellet using FLAG agarose beads. pnzhao@163.com, zhangshuye@ shphc.org.cn, zhangxiaoyan@shphc.org.cn, xujianqing@shphc.org.cn. the email address xujianqing@shphc.org.cn is n.b.g. Accepted 2022-JAN-10 AT 07:50 GMT AT 07:50 GMT by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation imex curation Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells. Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells. Proceedings of the National Academy of Sciences of the United States of America 2021 imex curation Chen J., Fan J., Chen Z., Zhang M., Peng H., Liu J., Ding L., Liu M., Zhao C., Zhao P., Zhang S., Zhang X., Xu J. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus email sent 22/12 requesting more info about "S606-APEX2-FLAG" + expression system/purity. The authors have stated that:- 1]"S606" is a truncated Spike protein (position 1-606 amino acid) of SARS-CoV-2 (UniProtKB - P0DTC2)，but not a mutation at residue 606. 2] Proximity labelling: Cells were treated with S606-APEX2-FLAG, as in the methods "APEX2" section; A fusion expression system was constructed: pSV1.0-S606-APEX-FLAG, was transfected into HEK293T cells. This fusion protein was purified from the cell pellet using FLAG agarose beads. pnzhao@163.com, zhangshuye@ shphc.org.cn, zhangxiaoyan@shphc.org.cn, xujianqing@shphc.org.cn. the email address xujianqing@shphc.org.cn is n.b.g. Only protein-protein interactions imex curation human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chen J., Fan J., Chen Z., Zhang M., Peng H., Liu J., Ding L., Liu M., Zhao C., Zhao P., Zhang S., Zhang X., Xu J. Virus - Publications including interactions involving viral proteins email sent 22/12 requesting more info about "S606-APEX2-FLAG" + expression system/purity. The authors have stated that:- 1]"S606" is a truncated Spike protein (position 1-606 amino acid) of SARS-CoV-2 (UniProtKB - P0DTC2)，but not a mutation at residue 606. 2] Proximity labelling: Cells were treated with S606-APEX2-FLAG, as in the methods "APEX2" section; A fusion expression system was constructed: pSV1.0-S606-APEX-FLAG, was transfected into HEK293T cells. This fusion protein was purified from the cell pellet using FLAG agarose beads. pnzhao@163.com, zhangshuye@ shphc.org.cn, zhangxiaoyan@shphc.org.cn, xujianqing@shphc.org.cn. the email address xujianqing@shphc.org.cn is n.b.g. Accepted 2022-JAN-10 AT 07:50 GMT AT 07:50 GMT by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation imex curation myh9_human Myosin-9 MYH9 Myosin heavy chain 9 Myosin heavy chain, non-muscle IIa Non-muscle myosin heavy chain IIa Cellular myosin heavy chain, type A Non-muscle myosin heavy chain A protein protein human Homo sapiens Human MAQQAADKYLYVDKNFINNPLAQADWAAKKLVWVPSDKSGFEPASLKEEVGEEAIVELVENGKKVKVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKERYYSGLIYTYSGLFCVVINPYKNLPIYSEEIVEMYKGKKRHEMPPHIYAITDTAYRSMMQDREDQSILCTGESGAGKTENTKKVIQYLAYVASSHKSKKDQGELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVNGYIVGANIETYLLEKSRAIRQAKEERTFHIFYYLLSGAGEHLKTDLLLEPYNKYRFLSNGHVTIPGQQDKDMFQETMEAMRIMGIPEEEQMGLLRVISGVLQLGNIVFKKERNTDQASMPDNTAAQKVSHLLGINVTDFTRGILTPRIKVGRDYVQKAQTKEQADFAIEALAKATYERMFRWLVLRINKALDKTKRQGASFIGILDIAGFEIFDLNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIEKPAGPPGILALLDEECWFPKATDKSFVEKVMQEQGTHPKFQKPKQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNIATLLHQSSDKFVSELWKDVDRIIGLDQVAGMSETALPGAFKTRKGMFRTVGQLYKEQLAKLMATLRNTNPNFVRCIIPNHEKKAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRVVFQEFRQRYEILTPNSIPKGFMDGKQACVLMIKALELDSNLYRIGQSKVFFRAGVLAHLEEERDLKITDVIIGFQACCRGYLARKAFAKRQQQLTAMKVLQRNCAAYLKLRNWQWWRLFTKVKPLLQVSRQEEEMMAKEEELVKVREKQLAAENRLTEMETLQSQLMAEKLQLQEQLQAETELCAEAEELRARLTAKKQELEEICHDLEARVEEEEERCQHLQAEKKKMQQNIQELEEQLEEEESARQKLQLEKVTTEAKLKKLEEEQIILEDQNCKLAKEKKLLEDRIAEFTTNLTEEEEKSKSLAKLKNKHEAMITDLEERLRREEKQRQELEKTRRKLEGDSTDLSDQIAELQAQIAELKMQLAKKEEELQAALARVEEEAAQKNMALKKIRELESQISELQEDLESERASRNKAEKQKRDLGEELEALKTELEDTLDSTAAQQELRSKREQEVNILKKTLEEEAKTHEAQIQEMRQKHSQAVEELAEQLEQTKRVKANLEKAKQTLENERGELANEVKVLLQGKGDSEHKRKKVEAQLQELQVKFNEGERVRTELADKVTKLQVELDNVTGLLSQSDSKSSKLTKDFSALESQLQDTQELLQEENRQKLSLSTKLKQVEDEKNSFREQLEEEEEAKHNLEKQIATLHAQVADMKKKMEDSVGCLETAEEVKRKLQKDLEGLSQRHEEKVAAYDKLEKTKTRLQQELDDLLVDLDHQRQSACNLEKKQKKFDQLLAEEKTISAKYAEERDRAEAEAREKETKALSLARALEEAMEQKAELERLNKQFRTEMEDLMSSKDDVGKSVHELEKSKRALEQQVEEMKTQLEELEDELQATEDAKLRLEVNLQAMKAQFERDLQGRDEQSEEKKKQLVRQVREMEAELEDERKQRSMAVAARKKLEMDLKDLEAHIDSANKNRDEAIKQLRKLQAQMKDCMRELDDTRASREEILAQAKENEKKLKSMEAEMIQLQEELAAAERAKRQAQQERDELADEIANSSGKGALALEEKRRLEARIAQLEEELEEEQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKVKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRTEKKLKDVLLQVDDERRNAEQYKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE DIP protein P35579 original sequence version: 126 DIP protein P35579-1 original sequence version: 142 DIP protein P35579-1 original sequence version: 133 DIP protein P35579 original sequence version: 186 DIP protein P35579-1 original sequence version: 186 DIP protein P35579-1 original sequence version: 146 DIP protein P35579 original sequence version: 146 588F84BB8C106E6F spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 spike_sars Spike glycoprotein S E2 Peplomer protein 2 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT DIP protein P59594 original sequence version: 48 s-myh9-1 5 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 9 unspecified role unspecified role bait bait partially purified partially purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined apex2 tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. proximity proximity close-contact colocalization neighbourhood interaction Figure 1, Supp. figure 1A, Supp table 1. In table S1 several proteins are detected, but as many of them are found in the CRAPOME database they have not been included as interactors here. s-myh9-2 3 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 9 unspecified role unspecified role bait bait partially purified partially purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined apex2 tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. proximity proximity close-contact colocalization neighbourhood interaction Figure 1A s-myh9-3 7 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range S1 region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association 1C S1 region; this appears to vary - different in Uniprot to other sources. I have taken the region from the S1 region given by Sinobiological in VG40591-CH from methods. myh9-s-1 1 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed S1-NTD sufficient to bind sufficient binding region sufficient to bind range range range range S2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range PRA sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2B, Supp figure 2B. i am assuming the positive band with Spike "NTD" in Figure S3A is "non-specific" as text describes this figure as a control. myh9-s-2 1 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range PRA sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2B myh9-s-3 6 8 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role neutral component neutral component infection infection colocalization colocalization 2C "ACE2-A549" cells were used. myh9-s-5 2 8 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role neutral component neutral component infection infection colocalization colocalization 2C myh9-s-6 4 8 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role neutral component neutral component infection infection colocalization colocalization 2C