IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ S19W, T27W, and N330Y mutations in ACE2 enhance SARS-CoV-2 S-RBD binding toward both wild-type and antibody-resistant viruses and its molecular basis. S19W, T27W, and N330Y mutations in ACE2 enhance SARS-CoV-2 S-RBD binding toward both wild-type and antibody-resistant viruses and its molecular basis. Signal transduction and targeted therapy 2021 imex curation Ye F., Lin X., Chen Z., Yang F., Lin S., Yang J., Chen H., Sun H., Wang L., Wen A., Zhang X., Dai Y., Cao Y., Yang J., Shen G., Yang L., Li J., Wang Z., Wang W., Wei X., Lu G. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus asked for pdbe entries to be made visible 29/9/21;Asked again 9/10/21 (and probably another time as well??) XRay data was curated from the publication; normally i wait until PDBe entries are visible - but this could be a very long time. 12/10/21; had email from David Armstrong (of Pdbe presumably) saying that:- "These entries were processed by our colleagues at PDBj so I have created a ticket for them to add this information and release the entries." lugw@scu.edu.cn Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Ye F., Lin X., Chen Z., Yang F., Lin S., Yang J., Chen H., Sun H., Wang L., Wen A., Zhang X., Dai Y., Cao Y., Yang J., Shen G., Yang L., Li J., Wang Z., Wang W., Wei X., Lu G. Virus - Publications including interactions involving viral proteins asked for pdbe entries to be made visible 29/9/21;Asked again 9/10/21 (and probably another time as well??) XRay data was curated from the publication; normally i wait until PDBe entries are visible - but this could be a very long time. 12/10/21; had email from David Armstrong (of Pdbe presumably) saying that:- "These entries were processed by our colleagues at PDBj so I have created a ticket for them to add this information and release the entries." lugw@scu.edu.cn Accepted 2021-NOV-24 AT 17:06 GMT AT 17:06 GMT by ORCHARD Signal transduction and targeted therapy 2021 Only protein-protein interactions imex curation imex curation S19W, T27W, and N330Y mutations in ACE2 enhance SARS-CoV-2 S-RBD binding toward both wild-type and antibody-resistant viruses and its molecular basis. S19W, T27W, and N330Y mutations in ACE2 enhance SARS-CoV-2 S-RBD binding toward both wild-type and antibody-resistant viruses and its molecular basis. Signal transduction and targeted therapy 2021 imex curation Ye F., Lin X., Chen Z., Yang F., Lin S., Yang J., Chen H., Sun H., Wang L., Wen A., Zhang X., Dai Y., Cao Y., Yang J., Shen G., Yang L., Li J., Wang Z., Wang W., Wei X., Lu G. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus asked for pdbe entries to be made visible 29/9/21;Asked again 9/10/21 (and probably another time as well??) XRay data was curated from the publication; normally i wait until PDBe entries are visible - but this could be a very long time. 12/10/21; had email from David Armstrong (of Pdbe presumably) saying that:- "These entries were processed by our colleagues at PDBj so I have created a ticket for them to add this information and release the entries." lugw@scu.edu.cn Only protein-protein interactions imex curation in vitro In vitro thermal shift thermal shift binding thermal shift predetermined predetermined participant predetermined Ye F., Lin X., Chen Z., Yang F., Lin S., Yang J., Chen H., Sun H., Wang L., Wen A., Zhang X., Dai Y., Cao Y., Yang J., Shen G., Yang L., Li J., Wang Z., Wang W., Wei X., Lu G. Virus - Publications including interactions involving viral proteins asked for pdbe entries to be made visible 29/9/21;Asked again 9/10/21 (and probably another time as well??) XRay data was curated from the publication; normally i wait until PDBe entries are visible - but this could be a very long time. 12/10/21; had email from David Armstrong (of Pdbe presumably) saying that:- "These entries were processed by our colleagues at PDBj so I have created a ticket for them to add this information and release the entries." DSF-Differential scanning fluorimetry:SYPRO Orange dye (Sigma) was used to probe protein thermal denaturation and the fluorescence signal as a function of temperature was monitored. lugw@scu.edu.cn Accepted 2021-NOV-24 AT 17:06 GMT AT 17:06 GMT by ORCHARD Signal transduction and targeted therapy 2021 Only protein-protein interactions imex curation imex curation S19W, T27W, and N330Y mutations in ACE2 enhance SARS-CoV-2 S-RBD binding toward both wild-type and antibody-resistant viruses and its molecular basis. S19W, T27W, and N330Y mutations in ACE2 enhance SARS-CoV-2 S-RBD binding toward both wild-type and antibody-resistant viruses and its molecular basis. Signal transduction and targeted therapy 2021 imex curation Ye F., Lin X., Chen Z., Yang F., Lin S., Yang J., Chen H., Sun H., Wang L., Wen A., Zhang X., Dai Y., Cao Y., Yang J., Shen G., Yang L., Li J., Wang Z., Wang W., Wei X., Lu G. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus asked for pdbe entries to be made visible 29/9/21;Asked again 9/10/21 (and probably another time as well??) XRay data was curated from the publication; normally i wait until PDBe entries are visible - but this could be a very long time. 12/10/21; had email from David Armstrong (of Pdbe presumably) saying that:- "These entries were processed by our colleagues at PDBj so I have created a ticket for them to add this information and release the entries." lugw@scu.edu.cn Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ye F., Lin X., Chen Z., Yang F., Lin S., Yang J., Chen H., Sun H., Wang L., Wen A., Zhang X., Dai Y., Cao Y., Yang J., Shen G., Yang L., Li J., Wang Z., Wang W., Wei X., Lu G. Virus - Publications including interactions involving viral proteins asked for pdbe entries to be made visible 29/9/21;Asked again 9/10/21 (and probably another time as well??) XRay data was curated from the publication; normally i wait until PDBe entries are visible - but this could be a very long time. 12/10/21; had email from David Armstrong (of Pdbe presumably) saying that:- "These entries were processed by our colleagues at PDBj so I have created a ticket for them to add this information and release the entries." lugw@scu.edu.cn Accepted 2021-NOV-24 AT 17:06 GMT AT 17:06 GMT by ORCHARD Signal transduction and targeted therapy 2021 Only protein-protein interactions imex curation imex curation ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT spike_sars Spike glycoprotein S E2 Peplomer protein 2 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT DIP protein P59594 original sequence version: 48 mln-4760 MLN-4760 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) s-ace2-98 1 4 unspecified role unspecified role prey prey purified purified peptidase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9BYF1:p.[Ser19Trp;Asn330Tyr;His374Ala;His378Ala;Glu402Ala] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N Y certain certain sequence position certain certain certain sequence position certain S W certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain E A Tue Oct 05 18:41:52 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser19Trp;Asn330Tyr;His374Ala; His378Ala;Glu402Ala]' Q9BYF1:p.[Thr27Trp;Asn330Tyr;His374Ala;His378Ala;Glu402Ala] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain T W certain certain sequence position certain certain certain sequence position certain N Y certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain E A Tue Oct 05 18:55:29 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr27Trp;Asn330Tyr;His374Ala; His378Ala;Glu402Ala]' Q9BYF1:p.[Ser19Trp;Thr27Trp;Asn330Tyr;His374Ala;His378Ala;Glu402Ala] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S W certain certain sequence position certain certain certain sequence position certain T W certain certain sequence position certain certain certain sequence position certain N Y certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain E A Tue Oct 05 18:50:58 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser19Trp;Thr27Trp;Asn330Tyr;His374Ala; His378Ala;Glu402Ala]' Q9BYF1:p.[Ser19Trp;Thr27Trp;His374Ala;His378Ala;Glu402Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S W certain certain sequence position certain certain certain sequence position certain T W certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain E A Tue Oct 05 18:59:14 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser19Trp;Thr27Trp;His374Ala; His378Ala;Glu402Ala]' spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 5 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction 1 s-ace2-99 1 4 unspecified role unspecified role prey prey purified purified peptidase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9BYF1:p.[Ser19Trp;Asn330Tyr;His374Ala;His378Ala;Glu402Ala] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N Y certain certain sequence position certain certain certain sequence position certain S W certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain E A Tue Oct 05 19:32:14 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser19Trp;Asn330Tyr;His374Ala; His378Ala;Glu402Ala]' Q9BYF1:p.[Thr27Trp;Asn330Tyr;His374Ala;His378Ala;Glu402Ala] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain T W certain certain sequence position certain certain certain sequence position certain N Y certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain E A Tue Oct 05 18:55:27 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr27Trp;Asn330Tyr;His374Ala; His378Ala;Glu402Ala]' Q9BYF1:p.[Ser19Trp;Thr27Trp;Asn330Tyr;His374Ala;His378Ala;Glu402Ala] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S W certain certain sequence position certain certain certain sequence position certain T W certain certain sequence position certain certain certain sequence position certain N Y certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain E A Tue Oct 05 19:22:41 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser19Trp;Thr27Trp;Asn330Tyr;His374Ala; His378Ala;Glu402Ala]' spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 6 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction 6 s-ace2-100 1 4 unspecified role unspecified role prey prey purified purified peptidase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9BYF1:p.Asn330Tyr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N Y Tue Oct 05 18:27:00 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn330Tyr' Q9BYF1:p.[His374Ala;His378Ala;Glu402Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain E A Tue Oct 05 19:13:15 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[His374Ala; His378Ala;Glu402Ala]' Q9BYF1:p.Ser19Trp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S W Tue Oct 05 19:14:29 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser19Trp' Q9BYF1:p.Thr27Trp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain T W Tue Oct 05 18:56:29 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr27Trp' Q9BYF1:p.His34Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain H E Tue Oct 05 18:54:43 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His34Glu' Q9BYF1:p.Gly326Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G K Tue Oct 05 18:50:11 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly326Lys' Q9BYF1:p.Lys353Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K R Tue Oct 05 19:13:09 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys353Arg' Q9BYF1:p.Gly326Trp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G W Tue Oct 05 19:34:54 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly326Trp' spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 5 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Supp. figure 3 entered as a separate interaction for clarity and hopefully to avoid errors. s-ace2-101 1 4 unspecified role unspecified role prey prey purified purified peptidase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 5 unspecified role unspecified role bait bait purified purified P0DTC2:p.[Lys417Asn;Glu484Lys;Asn501Tyr] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain K N certain certain sequence position certain certain certain sequence position certain E K certain certain sequence position certain certain certain sequence position certain N Y Tue Oct 05 18:25:44 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys417Asn;Glu484Lys;Asn501Tyr]' P0DTC2:p.Glu484Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E K Tue Oct 05 19:05:54 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu484Lys' P0DTC2:p.Phe490Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F L Tue Oct 05 19:19:03 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe490Leu' P0DTC2:p.Tyr508His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Y H Tue Oct 05 19:03:17 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr508His' P0DTC2:p.Asn439Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N K Tue Oct 05 19:03:05 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn439Lys' P0DTC2:p.Val483Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain V A Tue Oct 05 19:10:34 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val483Ala' P0DTC2:p.Gly446Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G V Tue Oct 05 19:03:38 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly446Val' P0DTC2:p.Asn450Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N K Tue Oct 05 18:56:13 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn450Lys' P0DTC2:p.Leu452Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain L R Tue Oct 05 19:36:38 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu452Arg' P0DTC2:p.Ala475Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A V Tue Oct 05 18:41:59 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala475Val' P0DTC2:p.Ser477Asn mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S N Tue Oct 05 19:29:23 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser477Asn' P0DTC2:p.[Leu452Arg;Glu484Lys] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E K certain certain sequence position certain certain certain sequence position certain L R Tue Oct 05 18:54:06 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu484Lys;Leu452Asn]' rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Supp. figure 6 s-ace2-102 1 4 unspecified role unspecified role prey prey purified purified Q9BYF1:p.[Ser19Trp;Asn330Tyr;His374Ala;His378Ala;Glu402Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain S W certain certain sequence position certain certain certain sequence position certain N Y certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain E A Tue Oct 05 18:57:34 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser19Trp;Asn330Tyr;His374Ala; His378Ala;Glu402Ala]' peptidase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 5 unspecified role unspecified role bait bait purified purified P0DTC2:p.Asn439Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N K Tue Oct 05 18:49:52 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn439Lys' P0DTC2:p.[Lys417Asn;Glu484Lys;Asn501Tyr] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain K N certain certain sequence position certain certain certain sequence position certain E K certain certain sequence position certain certain certain sequence position certain N Y Tue Oct 05 19:15:59 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys417Asn;Glu484Lys;Asn501Tyr]' P0DTC2:p.Glu484Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E K Tue Oct 05 19:15:18 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu484Lys' P0DTC2:p.Phe490Leu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain F L Tue Oct 05 18:45:21 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe490Leu' P0DTC2:p.Tyr508His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Y H Tue Oct 05 18:47:32 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr508His' P0DTC2:p.Val483Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain V A Tue Oct 05 19:37:26 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val483Ala' P0DTC2:p.Gly446Val mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain G V Tue Oct 05 18:45:33 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly446Val' P0DTC2:p.Asn450Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N K Tue Oct 05 18:35:38 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn450Lys' P0DTC2:p.Leu452Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain L R Tue Oct 05 18:55:26 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu452Arg' P0DTC2:p.Ala475Val mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain A V Tue Oct 05 19:01:00 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala475Val' P0DTC2:p.Ser477Asn mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S N Tue Oct 05 19:36:28 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser477Asn' P0DTC2:p.[Leu452Arg;Glu484Lys] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E K certain certain sequence position certain certain certain sequence position certain L R Tue Oct 05 18:51:27 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu484Lys;Leu452Asn]' rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Supp. figure 6-W19/Y330 mutant The descriptors "mutation increasing or decreasing" are in relation to the Kd value for WT Spike and WT ACE2 proteins (8.18 X E-8). s-ace2-103 1 4 unspecified role unspecified role prey prey purified purified Q9BYF1:p.[Thr27Trp;Asn330Tyr;His374Ala;His378Ala;Glu402Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain N Y certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain T W Tue Oct 05 19:25:26 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr27Trp;Asn330Tyr;His374Ala; His378Ala;Glu402Ala]' peptidase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 5 unspecified role unspecified role bait bait purified purified P0DTC2:p.Asn439Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N K Tue Oct 05 18:58:20 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn439Lys' P0DTC2:p.[Lys417Asn;Glu484Lys;Asn501Tyr] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain K N certain certain sequence position certain certain certain sequence position certain E K certain certain sequence position certain certain certain sequence position certain N Y Tue Oct 05 18:58:50 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys417Asn;Glu484Lys;Asn501Tyr]' P0DTC2:p.Glu484Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E K Tue Oct 05 19:07:09 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu484Lys' P0DTC2:p.Phe490Leu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain F L Tue Oct 05 19:14:30 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe490Leu' P0DTC2:p.Tyr508His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Y H Tue Oct 05 19:23:46 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr508His' P0DTC2:p.Val483Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain V A Tue Oct 05 18:53:25 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val483Ala' P0DTC2:p.Gly446Val mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain G V Tue Oct 05 19:32:18 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly446Val' P0DTC2:p.Asn450Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N K Tue Oct 05 19:00:12 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn450Lys' P0DTC2:p.Leu452Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain L R Tue Oct 05 18:46:36 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu452Arg' P0DTC2:p.Ala475Val mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain A V Tue Oct 05 18:42:24 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala475Val' P0DTC2:p.Ser477Asn mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S N Tue Oct 05 18:39:07 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser477Asn' P0DTC2:p.[Leu452Arg;Glu484Lys] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E K certain certain sequence position certain certain certain sequence position certain L R Tue Oct 05 19:11:32 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu484Lys;Leu452Asn]' rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Supp. figure 6-W27/Y330 mutant The descriptors "mutation increasing or decreasing" are in relation to the Kd value for WT Spike and WT ACE2 proteins (8.18 X E-8). s-ace2-104 1 4 unspecified role unspecified role prey prey purified purified Q9BYF1:p.[Ser19Trp;Thr27Trp;Asn330Tyr;His374Ala;His378Ala;Glu402Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain N Y certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain T W certain certain sequence position certain certain certain sequence position certain S W Tue Oct 05 18:38:22 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser19Trp;Thr27Trp;Asn330Tyr;His374Ala; His378Ala;Glu402Ala]' peptidase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 5 unspecified role unspecified role bait bait purified purified P0DTC2:p.Asn439Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N K Tue Oct 05 19:18:11 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn439Lys' P0DTC2:p.[Lys417Asn;Glu484Lys;Asn501Tyr] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain K N certain certain sequence position certain certain certain sequence position certain E K certain certain sequence position certain certain certain sequence position certain N Y Tue Oct 05 18:36:53 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys417Asn;Glu484Lys;Asn501Tyr]' P0DTC2:p.Glu484Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E K Tue Oct 05 18:51:15 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu484Lys' P0DTC2:p.Phe490Leu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain F L Tue Oct 05 19:05:20 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe490Leu' P0DTC2:p.Tyr508His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Y H Tue Oct 05 19:17:04 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr508His' P0DTC2:p.Val483Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain V A Tue Oct 05 19:30:42 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val483Ala' P0DTC2:p.Gly446Val mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain G V Tue Oct 05 19:27:25 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly446Val' P0DTC2:p.Asn450Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N K Tue Oct 05 18:49:27 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn450Lys' P0DTC2:p.Leu452Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain L R Tue Oct 05 18:43:00 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu452Arg' P0DTC2:p.Ala475Val mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain A V Tue Oct 05 18:38:47 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala475Val' P0DTC2:p.Ser477Asn mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S N Tue Oct 05 18:29:28 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser477Asn' P0DTC2:p.[Leu452Arg;Glu484Lys] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E K certain certain sequence position certain certain certain sequence position certain L R Tue Oct 05 18:29:51 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu484Lys;Leu452Asn]' rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Supp. figure 6-W19/W27/Y330 mutant The descriptors "mutation increasing or decreasing" are in relation to the Kd value for WT Spike and WT ACE2 proteins (8.18 X E-8). ace2-mln_4760-6 2 4 unspecified role unspecified role neutral component neutral component purified purified peptidase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 7 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Supp. figure 8 s-ace2-105 1 4 unspecified role unspecified role prey prey purified purified peptidase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 7 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Supp. figure 9 s-ace2-106 1 4 unspecified role unspecified role prey prey purified purified peptidase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 7 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Supp. figure 9 s-ace2-123 3 4 unspecified role unspecified role prey prey purified purified peptidase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9BYF1:p.[Thr27Trp;Asn330Tyr] mutation mutation certain certain sequence position certain certain certain sequence position certain T W certain certain sequence position certain certain certain sequence position certain N Y Thu Jan 06 19:36:02 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr27Trp;Asn330Tyr]' spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 5 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Supp. table 1 s-ace2-124 3 4 unspecified role unspecified role prey prey purified purified peptidase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9BYF1:p.[Ser19Trp;Asn330Tyr] mutation mutation certain certain sequence position certain certain certain sequence position certain N Y certain certain sequence position certain certain certain sequence position certain S W Thu Jan 06 19:37:07 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser19Trp;Asn330Tyr]' spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 5 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Supp. table 1