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  <entry>
    <source releaseDate="2022-02-04">
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        <shortLabel>IntAct</shortLabel>
        <fullName>European Bioinformatics Institute</fullName>
        <alias type="synonym" typeAc="MI:1041">IntAct</alias>
        <alias type="synonym" typeAc="MI:1041">intact</alias>
      </names>
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      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
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        <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
        <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
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        <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>A Comparative Analysis of Coronavirus Nucleocapsid (N) Proteins Reveals the SADS-CoV N Protein Antagonizes IFN-β Production by Inducing Ubiquitination of RIG-I.</fullName>
        </names>
        <bibref>
          <xref>
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          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">A Comparative Analysis of Coronavirus Nucleocapsid (N) Proteins Reveals the SADS-CoV N Protein Antagonizes IFN-β Production by Inducing Ubiquitination of RIG-I.</attribute>
            <attribute name="journal" nameAc="MI:0885">Frontiers in immunology</attribute>
            <attribute name="publication year" nameAc="MI:0886">2020</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Liu Y., Liang QZ., Lu W., Yang YL., Chen R., Huang YW., Wang B.</attribute>
            <attribute name="dataset" nameAc="MI:0875">Virus - Publications including interactions involving viral proteins</attribute>
            <attribute name="dataset" nameAc="MI:0875">Coronavirus - Interactions investigated in the context of Coronavirus</attribute>
            <attribute name="contact-email" nameAc="MI:0634">yhuang@zju.edu.cn,wb1990boy@163.com</attribute>
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        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="9606">
            <names>
              <shortLabel>human-293t</shortLabel>
              <fullName>Homo sapiens 293 cells transformed with SV40 large T antigen</fullName>
            </names>
            <cellType>
              <names>
                <shortLabel>293t</shortLabel>
                <fullName>293 cells expressing SV40 large T antigen.</fullName>
              </names>
              <xref>
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              </xref>
              <attributeList>
                <attribute name="comment" nameAc="MI:0612">The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB.</attribute>
              </attributeList>
            </cellType>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>anti tag coip</shortLabel>
            <fullName>anti tag coimmunoprecipitation</fullName>
            <alias type="synonym" typeAc="MI:1041">anti tag coip</alias>
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        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>anti tag western</shortLabel>
            <fullName>anti tag western blot</fullName>
            <alias type="synonym" typeAc="MI:1041">anti tag western</alias>
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          <attribute name="author-list" nameAc="MI:0636">Liu Y., Liang QZ., Lu W., Yang YL., Chen R., Huang YW., Wang B.</attribute>
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          <attribute name="accepted">Accepted 2021-OCT-18 AT 11:52 BST AT 11:52 BST by SIMONA</attribute>
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          <attribute name="publication year" nameAc="MI:0886">2020</attribute>
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        <names>
          <shortLabel>ddx58_human</shortLabel>
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          <alias type="gene name synonym" typeAc="MI:0302">Retinoic acid-inducible gene 1 protein</alias>
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          <alias type="gene name synonym" typeAc="MI:0302">Probable ATP-dependent RNA helicase DDX58</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Retinoic acid-inducible gene I protein</alias>
          <alias type="gene name synonym" typeAc="MI:0302">RIG-I-like receptor 1</alias>
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        <interactorType>
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        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
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        <attributeList>
          <attribute name="crc64">BF0D501C395BAE25</attribute>
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      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>a0a2s1wcf9_9alpc</shortLabel>
          <fullName>Nucleoprotein</fullName>
          <alias type="gene name" typeAc="MI:0301">N</alias>
        </names>
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            <fullName>protein</fullName>
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        </interactorType>
        <organism ncbiTaxId="2018513">
          <names>
            <shortLabel>Porcine enteric alphacoronavirus</shortLabel>
            <fullName>Porcine enteric alphacoronavirus</fullName>
          </names>
        </organism>
        <sequence>MATVNWGDAVEQAESRGRKRIPLSLFAPLRVIDGKNFWNVMPRNGVPTGKGTPDQQIGYWVEQKRWRMQKGQRKDQPSNWHFYYLGTGPHADAPFRKRIQGVHWVAVDGAKTSPTGLGVRNRNKEPATPQFGFQLPPDLTVVEVTSRSASRSQSRSRNQSQSRSGAQTPRAQQPSQSVDIVAAVKQALADLGIASSQSRPQSGKNTPKPRSRAVSPAPAPKPARKQMDKPEWKRVPNSGEDVLKCFGPRSVSRNFGDSDLVQHGVEAKHFPTIAELLPTQAALAFGSEITTKESGEFVEVTYHYVMKVPKTDKNLPRFLEQVSAYSKPSQIRRSQSQQDLNADAPVFTPAPPATPVSQNPAFLEEEVEMVDEIIN</sequence>
        <attributeList>
          <attribute name="crc64">E59D53D83B167A8F</attribute>
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      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4" imexId="IM-29083-1">
        <names>
          <shortLabel>ddx58-n-4</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-27032938" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-29083-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>over-expressed</shortLabel>
                  <fullName>over expressed level</fullName>
                  <alias type="synonym" typeAc="MI:1041">over-expressed</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0506" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537736" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <featureList>
              <feature id="6">
                <names>
                  <shortLabel>C-terminus</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-27032956" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>flag tag</shortLabel>
                    <fullName>flag tag</fullName>
                    <alias type="go synonym" typeAc="MI:0303">FLAG-tagged</alias>
                    <alias type="go synonym" typeAc="MI:0303">FLAG</alias>
                    <alias type="go synonym" typeAc="MI:0303">DYKDDDDKV epitope tag</alias>
                    <alias type="synonym" typeAc="MI:1041">DYKDDDDKV epitope tag</alias>
                    <alias type="synonym" typeAc="MI:1041">FLAG</alias>
                    <alias type="synonym" typeAc="MI:1041">FLAG-tagged</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0518" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456503" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>c-term range</shortLabel>
                        <fullName>C-terminal range</fullName>
                        <alias type="synonym" typeAc="MI:1041">c-term range</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1039" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-2929799" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14760721" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <endStatus>
                      <names>
                        <shortLabel>c-term range</shortLabel>
                        <fullName>C-terminal range</fullName>
                        <alias type="synonym" typeAc="MI:1041">c-term range</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1039" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-2929799" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14760721" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
          </participant>
          <participant id="7">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>over-expressed</shortLabel>
                  <fullName>over expressed level</fullName>
                  <alias type="synonym" typeAc="MI:1041">over-expressed</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0506" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537736" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <featureList>
              <feature id="8">
                <names>
                  <shortLabel>region</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-27032954" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>myc tag</shortLabel>
                    <fullName>myc tag</fullName>
                    <alias type="go synonym" typeAc="MI:0303">EUKLISEED epitope tag</alias>
                    <alias type="synonym" typeAc="MI:1041">EUKLISEED epitope tag</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0522" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456561" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>undetermined</shortLabel>
                        <fullName>undetermined sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">undetermined</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <endStatus>
                      <names>
                        <shortLabel>undetermined</shortLabel>
                        <fullName>undetermined sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">undetermined</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">4</attribute>
          <attribute name="comment" nameAc="MI:0612">The interaction was not RNA dependent.</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>