IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ V367F mutation in SARS-CoV-2 spike RBD emerging during the early transmission phase enhances viral infectivity through increased human ACE2 receptor binding affinity. V367F mutation in SARS-CoV-2 spike RBD emerging during the early transmission phase enhances viral infectivity through increased human ACE2 receptor binding affinity. Journal of virology 2021 imex curation Ou J., Zhou Z., Dai R., Zhang J., Zhao S., Wu X., Lan W., Ren Y., Cui L., Lan Q., Lu L., Seto D., Chodosh J., Wu J., Zhang G., Zhang Q. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus the ACE2 protein; no details of where obtained from so no tags/expression system/sequence length. zhangqw@jnu.edu.cn,zhanggong@jnu.edu.cn Only protein-protein interactions imex curation in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Ou J., Zhou Z., Dai R., Zhang J., Zhao S., Wu X., Lan W., Ren Y., Cui L., Lan Q., Lu L., Seto D., Chodosh J., Wu J., Zhang G., Zhang Q. Virus - Publications including interactions involving viral proteins the ACE2 protein; no details of where obtained from so no tags/expression system/sequence length. zhangqw@jnu.edu.cn,zhanggong@jnu.edu.cn Accepted 2021-JUL-05 AT 09:39 BST AT 09:39 BST by ORCHARD Journal of virology 2021 Only protein-protein interactions imex curation imex curation V367F mutation in SARS-CoV-2 spike RBD emerging during the early transmission phase enhances viral infectivity through increased human ACE2 receptor binding affinity. V367F mutation in SARS-CoV-2 spike RBD emerging during the early transmission phase enhances viral infectivity through increased human ACE2 receptor binding affinity. Journal of virology 2021 imex curation Ou J., Zhou Z., Dai R., Zhang J., Zhao S., Wu X., Lan W., Ren Y., Cui L., Lan Q., Lu L., Seto D., Chodosh J., Wu J., Zhang G., Zhang Q. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus the ACE2 protein; no details of where obtained from so no tags/expression system/sequence length. zhangqw@jnu.edu.cn,zhanggong@jnu.edu.cn Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Ou J., Zhou Z., Dai R., Zhang J., Zhao S., Wu X., Lan W., Ren Y., Cui L., Lan Q., Lu L., Seto D., Chodosh J., Wu J., Zhang G., Zhang Q. Virus - Publications including interactions involving viral proteins the ACE2 protein; no details of where obtained from so no tags/expression system/sequence length. zhangqw@jnu.edu.cn,zhanggong@jnu.edu.cn Accepted 2021-JUL-05 AT 09:39 BST AT 09:39 BST by ORCHARD Journal of virology 2021 Only protein-protein interactions imex curation imex curation spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 ace2-s-195 1 4 unspecified role unspecified role bait bait purified purified 3 unspecified role unspecified role prey prey partially purified partially purified region_minus_RRAR,TM and CD. sufficient to bind sufficient binding region sufficient to bind range range range range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range p.Arg682_Arg685del mutation mutation certain certain sequence position certain certain certain sequence position certain RRAR . P0DTC2:p.Val367Phe mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain V F Tue Oct 05 19:22:05 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val367Phe' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 5A Spike protein:is stated to lack "the furin cleavage site, TM and cytoplasmic domains". The exact sequence is not given. The furin cleavage site (usually stated to be "RRAR") has been entered here as a deletion;however it is unclear if this site was completely deleted, or as more usual, replaced with sequence GSAS. ace2-s-196 2 4 unspecified role unspecified role prey prey purified purified 3 unspecified role unspecified role bait bait partially purified partially purified region_minus_RRAR,TM and CD. sufficient to bind sufficient binding region sufficient to bind range range range range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.Arg682_Arg685del mutation mutation certain certain sequence position certain certain certain sequence position certain RRAR . P0DTC2:p.Val367Phe mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain V F Tue Oct 05 18:49:32 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val367Phe' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 5B Spike protein:is stated to lack "the furin cleavage site, TM and cytoplasmic domains". The exact sequence is not given. The furin cleavage site (usually stated to be "RRAR") has been entered here as a deletion;however it is unclear if this site was completely deleted, or as more usual, replaced with sequence GSAS.