IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Conformational dynamics of SARS-CoV-2 trimeric spike glycoprotein in complex with receptor ACE2 revealed by cryo-EM. Conformational dynamics of SARS-CoV-2 trimeric spike glycoprotein in complex with receptor ACE2 revealed by cryo-EM. Science advances 2020 imex curation Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y., Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus cong@sibcb.ac.cn,huangzhong@ips.ac.cn 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y., Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y. Virus - Publications including interactions involving viral proteins The complex structure was determined in the presence and absence of a trace amount of cross linker, glutaraldehyde . The two maps are in comparable conformation, suggesting that addition of cross linker did not change the conformation of the complex. cong@sibcb.ac.cn,huangzhong@ips.ac.cn Accepted 2020-DEC-21 AT 07:12 GMT AT 07:12 GMT by KALPANAP Science advances 2020 Only protein-protein interactions imex curation imex curation Conformational dynamics of SARS-CoV-2 trimeric spike glycoprotein in complex with receptor ACE2 revealed by cryo-EM. Conformational dynamics of SARS-CoV-2 trimeric spike glycoprotein in complex with receptor ACE2 revealed by cryo-EM. Science advances 2020 imex curation Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y., Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus cong@sibcb.ac.cn,huangzhong@ips.ac.cn 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 3d-em-single electron microscopy 3D single particle reconstruction 3D-EM-single structure determination identification by structure determination identification by structure determination structure determination Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y., Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y. Virus - Publications including interactions involving viral proteins cong@sibcb.ac.cn,huangzhong@ips.ac.cn Accepted 2020-DEC-21 AT 07:12 GMT AT 07:12 GMT by KALPANAP Science advances 2020 Only protein-protein interactions imex curation imex curation Conformational dynamics of SARS-CoV-2 trimeric spike glycoprotein in complex with receptor ACE2 revealed by cryo-EM. Conformational dynamics of SARS-CoV-2 trimeric spike glycoprotein in complex with receptor ACE2 revealed by cryo-EM. Science advances 2020 imex curation Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y., Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus cong@sibcb.ac.cn,huangzhong@ips.ac.cn 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y., Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y. Virus - Publications including interactions involving viral proteins cong@sibcb.ac.cn,huangzhong@ips.ac.cn Accepted 2020-DEC-21 AT 07:12 GMT AT 07:12 GMT by KALPANAP Science advances 2020 Only protein-protein interactions imex curation imex curation Conformational dynamics of SARS-CoV-2 trimeric spike glycoprotein in complex with receptor ACE2 revealed by cryo-EM. Conformational dynamics of SARS-CoV-2 trimeric spike glycoprotein in complex with receptor ACE2 revealed by cryo-EM. Science advances 2020 imex curation Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y., Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus cong@sibcb.ac.cn,huangzhong@ips.ac.cn 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y., Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y. Virus - Publications including interactions involving viral proteins cong@sibcb.ac.cn,huangzhong@ips.ac.cn Accepted 2020-DEC-21 AT 07:12 GMT AT 07:12 GMT by KALPANAP Science advances 2020 Only protein-protein interactions imex curation imex curation Conformational dynamics of SARS-CoV-2 trimeric spike glycoprotein in complex with receptor ACE2 revealed by cryo-EM. Conformational dynamics of SARS-CoV-2 trimeric spike glycoprotein in complex with receptor ACE2 revealed by cryo-EM. Science advances 2020 imex curation Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y., Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus cong@sibcb.ac.cn,huangzhong@ips.ac.cn 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro 3d-em-single electron microscopy 3D single particle reconstruction 3D-EM-single structure determination identification by structure determination identification by structure determination structure determination Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y., Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y. Virus - Publications including interactions involving viral proteins The complex structure was determined in the presence and absence of a trace amount of cross linker, glutaraldehyde . The two maps are in comparable conformation, suggesting that addition of cross linker did not change the conformation of the complex. cong@sibcb.ac.cn,huangzhong@ips.ac.cn Accepted 2020-DEC-21 AT 07:12 GMT AT 07:12 GMT by KALPANAP Science advances 2020 Only protein-protein interactions imex curation imex curation Conformational dynamics of SARS-CoV-2 trimeric spike glycoprotein in complex with receptor ACE2 revealed by cryo-EM. Conformational dynamics of SARS-CoV-2 trimeric spike glycoprotein in complex with receptor ACE2 revealed by cryo-EM. Science advances 2020 imex curation Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y., Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus cong@sibcb.ac.cn,huangzhong@ips.ac.cn 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y., Xu C., Wang Y., Liu C., Zhang C., Han W., Hong X., Wang Y., Hong Q., Wang S., Zhao Q., Wang Y., Yang Y., Chen K., Zheng W., Kong L., Wang F., Zuo Q., Huang Z., Cong Y. Virus - Publications including interactions involving viral proteins cong@sibcb.ac.cn,huangzhong@ips.ac.cn Accepted 2020-DEC-21 AT 07:12 GMT AT 07:12 GMT by KALPANAP Science advances 2020 Only protein-protein interactions imex curation imex curation ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT s-60 2 8 unspecified role unspecified role neutral component neutral component purified purified P0DTC2:p.[Arg682_Arg683delinsGlySer;Arg685Ser;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain R S certain certain sequence position certain certain certain sequence position certain KV PP certain certain sequence position certain certain certain sequence position certain RR GS Sun Dec 13 19:48:33 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.[Lys986_Val987delinsProPro;Arg682_Arg683delinsGlySer;Arg685Ser]' T4 fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range direct interaction direct interaction Table S1 s-62 5 7 unspecified role unspecified role neutral component neutral component purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range IL10_signal_sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range peptidase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 8 unspecified role unspecified role neutral component neutral component purified purified P0DTC2:p.[Arg682_Arg683delinsGlySer;Arg685Ser;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain R S certain certain sequence position certain certain certain sequence position certain KV PP certain certain sequence position certain certain certain sequence position certain RR GS Sun Dec 13 19:48:33 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.[Lys986_Val987delinsProPro;Arg682_Arg683delinsGlySer;Arg685Ser]' T4 fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Table S1 ace2-s-79 3 7 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range IL10_signal_sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 8 unspecified role unspecified role bait bait purified purified IL10_signal_sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0DTC2:p.Tyr505Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y A rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0DTC2:p.Gln498Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q A C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.Val503Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V A P0DTC2:p.Thr470_Thr478delinsAsnValProPheSerProAspGlyLys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain TEIYQAGST NVPFSPDGK human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Figure 3C/D, Supp. Figure 7 I have included mutation for RBM-R3 even though it is a chimera, as it can be entered as a "mutation". ace2-s-80 6 7 unspecified role unspecified role neutral component neutral component purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range IL10_signal_sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range peptidase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 8 unspecified role unspecified role neutral component neutral component purified purified P0DTC2:p.[Arg682_Arg683delinsGlySer;Arg685Ser;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain R S certain certain sequence position certain certain certain sequence position certain KV PP certain certain sequence position certain certain certain sequence position certain RR GS Sun Dec 13 19:48:33 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.[Lys986_Val987delinsProPro;Arg682_Arg683delinsGlySer;Arg685Ser]' T4 fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Figure S1D ace2-s-81 4 8 unspecified role unspecified role neutral component neutral component purified purified P0DTC2:p.[Arg682_Arg683delinsGlySer;Arg685Ser;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain R S certain certain sequence position certain certain certain sequence position certain KV PP certain certain sequence position certain certain certain sequence position certain RR GS Sun Dec 13 19:48:33 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.[Lys986_Val987delinsProPro;Arg682_Arg683delinsGlySer;Arg685Ser]' T4 fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role neutral component neutral component purified purified P0DTC2:p.[Arg682_Arg683delinsGlySer;Arg685Ser;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain R S certain certain sequence position certain certain certain sequence position certain KV PP certain certain sequence position certain certain certain sequence position certain RR GS Sun Dec 13 19:48:33 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.[Lys986_Val987delinsProPro;Arg682_Arg683delinsGlySer;Arg685Ser]' T4 fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range direct interaction direct interaction Figure S1B No Stoich. entered as the M.wt of the Spike monomer is 134kDa - so no idea what theM. wt is meant to be here, allowing for possible increase due to glycosylation. ace2-s-82 1 7 unspecified role unspecified role neutral component neutral component purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range IL10_signal_sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range peptidase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 8 unspecified role unspecified role bait bait purified purified T4 fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.[Arg682_Arg683delinsGlySer;Arg685Ser;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain R S certain certain sequence position certain certain certain sequence position certain KV PP certain certain sequence position certain certain certain sequence position certain RR GS Sun Dec 13 19:48:33 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.[Lys986_Val987delinsProPro;Arg682_Arg683delinsGlySer;Arg685Ser]' C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Figure S1E