IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SARS-CoV-2 membrane glycoprotein M antagonizes the MAVS-mediated innate antiviral response. SARS-CoV-2 membrane glycoprotein M antagonizes the MAVS-mediated innate antiviral response. Cellular & molecular immunology 2020 imex curation Fu YZ., Wang SY., Zheng ZQ., Yi Huang null., Li WW., Xu ZS., Wang YY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Results from figures 4C and 4D have not been included as the species origins of FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 cannot be traced. The authors did not reply to a request for this information. sometimes they use 293 cells, sometimes 293T cells, for no apparent reason - I have separated out the experiments for the use of each cell line. email sent 18/11/20:-FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 as used in Figure 4 C and D MAVS (VISA) was determined to be of Human origin;PMID: 16153868 (via Ref. 43). FLAG-SNX8 is of Human origin, based on sequence length (465aa) from Ref. 31, figure 5, pmid: 31511639. yuzhi.fu@wh.iov.cn,wangyy@wh.iov.cn 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Fu YZ., Wang SY., Zheng ZQ., Yi Huang null., Li WW., Xu ZS., Wang YY. Virus - Publications including interactions involving viral proteins Results from figures 4C and 4D have not been included as the species origins of FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 cannot be traced. The authors did not reply to a request for this information. sometimes they use 293 cells, sometimes 293T cells, for no apparent reason - I have separated out the experiments for the use of each cell line. email sent 18/11/20:-FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 as used in Figure 4 C and D MAVS (VISA) was determined to be of Human origin;PMID: 16153868 (via Ref. 43). FLAG-SNX8 is of Human origin, based on sequence length (465aa) from Ref. 31, figure 5, pmid: 31511639. yuzhi.fu@wh.iov.cn,wangyy@wh.iov.cn Accepted 2020-DEC-08 AT 09:06 GMT AT 09:06 GMT by ORCHARD Cellular & molecular immunology 2020 Only protein-protein interactions imex curation imex curation SARS-CoV-2 membrane glycoprotein M antagonizes the MAVS-mediated innate antiviral response. SARS-CoV-2 membrane glycoprotein M antagonizes the MAVS-mediated innate antiviral response. Cellular & molecular immunology 2020 imex curation Fu YZ., Wang SY., Zheng ZQ., Yi Huang null., Li WW., Xu ZS., Wang YY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Results from figures 4C and 4D have not been included as the species origins of FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 cannot be traced. The authors did not reply to a request for this information. sometimes they use 293 cells, sometimes 293T cells, for no apparent reason - I have separated out the experiments for the use of each cell line. email sent 18/11/20:-FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 as used in Figure 4 C and D MAVS (VISA) was determined to be of Human origin;PMID: 16153868 (via Ref. 43). FLAG-SNX8 is of Human origin, based on sequence length (465aa) from Ref. 31, figure 5, pmid: 31511639. yuzhi.fu@wh.iov.cn,wangyy@wh.iov.cn 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Fu YZ., Wang SY., Zheng ZQ., Yi Huang null., Li WW., Xu ZS., Wang YY. Virus - Publications including interactions involving viral proteins Results from figures 4C and 4D have not been included as the species origins of FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 cannot be traced. The authors did not reply to a request for this information. sometimes they use 293 cells, sometimes 293T cells, for no apparent reason - I have separated out the experiments for the use of each cell line. email sent 18/11/20:-FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 as used in Figure 4 C and D MAVS (VISA) was determined to be of Human origin;PMID: 16153868 (via Ref. 43). FLAG-SNX8 is of Human origin, based on sequence length (465aa) from Ref. 31, figure 5, pmid: 31511639. yuzhi.fu@wh.iov.cn,wangyy@wh.iov.cn Accepted 2020-DEC-08 AT 09:06 GMT AT 09:06 GMT by ORCHARD Cellular & molecular immunology 2020 Only protein-protein interactions imex curation imex curation SARS-CoV-2 membrane glycoprotein M antagonizes the MAVS-mediated innate antiviral response. SARS-CoV-2 membrane glycoprotein M antagonizes the MAVS-mediated innate antiviral response. Cellular & molecular immunology 2020 imex curation Fu YZ., Wang SY., Zheng ZQ., Yi Huang null., Li WW., Xu ZS., Wang YY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Results from figures 4C and 4D have not been included as the species origins of FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 cannot be traced. The authors did not reply to a request for this information. sometimes they use 293 cells, sometimes 293T cells, for no apparent reason - I have separated out the experiments for the use of each cell line. email sent 18/11/20:-FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 as used in Figure 4 C and D MAVS (VISA) was determined to be of Human origin;PMID: 16153868 (via Ref. 43). FLAG-SNX8 is of Human origin, based on sequence length (465aa) from Ref. 31, figure 5, pmid: 31511639. yuzhi.fu@wh.iov.cn,wangyy@wh.iov.cn 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Fu YZ., Wang SY., Zheng ZQ., Yi Huang null., Li WW., Xu ZS., Wang YY. Virus - Publications including interactions involving viral proteins Results from figures 4C and 4D have not been included as the species origins of FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 cannot be traced. The authors did not reply to a request for this information. sometimes they use 293 cells, sometimes 293T cells, for no apparent reason - I have separated out the experiments for the use of each cell line. email sent 18/11/20:-FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 as used in Figure 4 C and D MAVS (VISA) was determined to be of Human origin;PMID: 16153868 (via Ref. 43). FLAG-SNX8 is of Human origin, based on sequence length (465aa) from Ref. 31, figure 5, pmid: 31511639. yuzhi.fu@wh.iov.cn,wangyy@wh.iov.cn Accepted 2020-DEC-08 AT 09:06 GMT AT 09:06 GMT by ORCHARD Cellular & molecular immunology 2020 Only protein-protein interactions imex curation imex curation SARS-CoV-2 membrane glycoprotein M antagonizes the MAVS-mediated innate antiviral response. SARS-CoV-2 membrane glycoprotein M antagonizes the MAVS-mediated innate antiviral response. Cellular & molecular immunology 2020 imex curation Fu YZ., Wang SY., Zheng ZQ., Yi Huang null., Li WW., Xu ZS., Wang YY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Results from figures 4C and 4D have not been included as the species origins of FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 cannot be traced. The authors did not reply to a request for this information. sometimes they use 293 cells, sometimes 293T cells, for no apparent reason - I have separated out the experiments for the use of each cell line. email sent 18/11/20:-FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 as used in Figure 4 C and D MAVS (VISA) was determined to be of Human origin;PMID: 16153868 (via Ref. 43). FLAG-SNX8 is of Human origin, based on sequence length (465aa) from Ref. 31, figure 5, pmid: 31511639. yuzhi.fu@wh.iov.cn,wangyy@wh.iov.cn 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Fu YZ., Wang SY., Zheng ZQ., Yi Huang null., Li WW., Xu ZS., Wang YY. Virus - Publications including interactions involving viral proteins Results from figures 4C and 4D have not been included as the species origins of FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 cannot be traced. The authors did not reply to a request for this information. sometimes they use 293 cells, sometimes 293T cells, for no apparent reason - I have separated out the experiments for the use of each cell line. email sent 18/11/20:-FLAG tagged RIG1, MDA5, TBK1, IRF3, TRAF3 as used in Figure 4 C and D MAVS (VISA) was determined to be of Human origin;PMID: 16153868 (via Ref. 43). FLAG-SNX8 is of Human origin, based on sequence length (465aa) from Ref. 31, figure 5, pmid: 31511639. yuzhi.fu@wh.iov.cn,wangyy@wh.iov.cn Accepted 2020-DEC-08 AT 09:06 GMT AT 09:06 GMT by ORCHARD Cellular & molecular immunology 2020 Only protein-protein interactions imex curation imex curation mavs_human Mitochondrial antiviral-signaling protein Interferon beta promoter stimulator protein 1 Virus-induced-signaling adapter CARD adapter inducing interferon beta Putative NF-kappa-B-activating protein 031N MAVS IPS1 KIAA1271 VISA protein protein human Homo sapiens Human MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYQSYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEQDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPSKLPINSTRAGMVPSKVPTSMVLTKVSASTVPTDGSSRNEETPAAPTPAGATGGSSAWLDSSSENRGLGSELSKPGVLASQVDSPFSGCFEDLAISASTSLGMGPCHGPEENEYKSEGTFGIHVAENPSIQLLEGNPGPPADPDGGPRPQADRKFQEREVPCHRPSPGALWLQVAVTGVLVVTLLVVLYRRRLH 0E23E3E115941EE8 vme1_sars2 Membrane protein E1 glycoprotein Matrix glycoprotein Membrane glycoprotein M protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MADSNGTITVEELKKLLEQWNLVIGFLFLTWICLLQFAYANRNRFLYIIKLIFLWLLWPVTLACFVLAAVYRINWITGGIAIAMACLVGLMWLSYFIASFRLFARTRSMWSFNPETNILLNVPLHGTILTRPLLESELVIGAVILRGHLRIAGHHLGRCDIKDLPKEITVATSRTLSYYKLGASQRVAGDSGFAAYSRYRIGNYKLNTDHSSSSDNIALLVQ snx8_human Sorting nexin-8 SNX8 protein protein human Homo sapiens Human MTGRAMDPLPAAAVGAAAEAEADEEADPPASDLPTPQAIEPQAIVQQVPAPSRMQMPQGNPLLLSHTLQELLARDTVQVELIPEKKGLFLKHVEYEVSSQRFKSSVYRRYNDFVVFQEMLLHKFPYRMVPALPPKRMLGADREFIEARRRALKRFVNLVARHPLFSEDVVLKLFLSFSGSDVQNKLKESAQCVGDEFLNCKLATRAKDFLPADIQAQFAISRELIRNIYNSFHKLRDRAERIASRAIDNAADLLIFGKELSAIGSDTTPLPSWAALNSSTWGSLKQALKGLSVEFALLADKAAQQGKQEENDVVEKLNLFLDLLQSYKDLCERHEKGVLHKHQRALHKYSLMKRQMMSATAQNREPESVEQLESRIVEQENAIQTMELRNYFSLYCLHQETQLIHVYLPLTSHILRAFVNSQIQGHKEMSKVWNDLRPKLSCLFAGPHSTLTPPCSPPEDGLCPH 90C5EDB761C31E88 vme1_sars2-mavs-1 2 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3A mavs-vme1_sars2-1 2 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3B mavs-vme1_sars2-2 4 5 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3C vme1_sars2-mavs-2 1 5 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 3D vme1_sars2-mavs-4 3 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined C-terminal region_deltaTM1/2/3 sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined N-terminal region, TM1/2 sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3E-RHS All the examined M truncations interacted with MAVS. mavs-vme1_sars2-3 3 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3E-LHS mavs-mavs-1 2 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4B The M protein reduced the self-association of MAVS. SARS2 M protein (P0DTC5) mavs-snx8-1 2 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4B The M protein reduced the self-association of MAVS and its association with SNX8. SARS2 M protein( P0DTC5)