IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Neuropilin-1 is a host factor for SARS-CoV-2 infection. Neuropilin-1 is a host factor for SARS-CoV-2 infection. Science (New York, N.Y.) 2020 imex curation Daly JL., Simonetti B., Klein K., Chen KE., Williamson MK., Antón-Plágaro C., Shoemark DK., Simón-Gracia L., Bauer M., Hollandi R., Greber UF., Horvath P., Sessions RB., Helenius A., Hiscox JA., Teesalu T., Matthews DA., Davidson AD., Collins BM., Cullen PJ., Yamauchi Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus emailed authors again 17/11 to address Sandra's point. The authors have stated that:- 1] the mCherry-NRP1 b1 domain is a human derived construct. The construct corresponds to residues 274-428 of H.sapiens NRP1. 2] the mCherry-NRP2 is also human derived. 3] The SARS2 SPIKE protein "S1 region" - corresponds to residues 13-685, corresponding to the S1 protein without the signal peptide. 4] The (full length) NRP1-mCherry used in Figure 1B is Human, while the Nrp1-mCherry used in Supp. figure 1D is the Mouse protein (gift from Donatella Valdembri). bs13866@bristol.ac.uk, pete.cullen@bristol.ac.uk, yohei.yamauchi@bristol.ac.uk 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Daly JL., Simonetti B., Klein K., Chen KE., Williamson MK., Antón-Plágaro C., Shoemark DK., Simón-Gracia L., Bauer M., Hollandi R., Greber UF., Horvath P., Sessions RB., Helenius A., Hiscox JA., Teesalu T., Matthews DA., Davidson AD., Collins BM., Cullen PJ., Yamauchi Y. Virus - Publications including interactions involving viral proteins emailed authors again 17/11 to address Sandra's point. The authors have stated that:- 1] the mCherry-NRP1 b1 domain is a human derived construct. The construct corresponds to residues 274-428 of H.sapiens NRP1. 2] the mCherry-NRP2 is also human derived. 3] The SARS2 SPIKE protein "S1 region" - corresponds to residues 13-685, corresponding to the S1 protein without the signal peptide. 4] The (full length) NRP1-mCherry used in Figure 1B is Human, while the Nrp1-mCherry used in Supp. figure 1D is the Mouse protein (gift from Donatella Valdembri). bs13866@bristol.ac.uk, pete.cullen@bristol.ac.uk, yohei.yamauchi@bristol.ac.uk Accepted 2020-NOV-19 AT 09:17 GMT AT 09:17 GMT by ORCHARD Science (New York, N.Y.) 2020 Only protein-protein interactions imex curation imex curation Neuropilin-1 is a host factor for SARS-CoV-2 infection. Neuropilin-1 is a host factor for SARS-CoV-2 infection. Science (New York, N.Y.) 2020 imex curation Daly JL., Simonetti B., Klein K., Chen KE., Williamson MK., Antón-Plágaro C., Shoemark DK., Simón-Gracia L., Bauer M., Hollandi R., Greber UF., Horvath P., Sessions RB., Helenius A., Hiscox JA., Teesalu T., Matthews DA., Davidson AD., Collins BM., Cullen PJ., Yamauchi Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus emailed authors again 17/11 to address Sandra's point. The authors have stated that:- 1] the mCherry-NRP1 b1 domain is a human derived construct. The construct corresponds to residues 274-428 of H.sapiens NRP1. 2] the mCherry-NRP2 is also human derived. 3] The SARS2 SPIKE protein "S1 region" - corresponds to residues 13-685, corresponding to the S1 protein without the signal peptide. 4] The (full length) NRP1-mCherry used in Figure 1B is Human, while the Nrp1-mCherry used in Supp. figure 1D is the Mouse protein (gift from Donatella Valdembri). bs13866@bristol.ac.uk, pete.cullen@bristol.ac.uk, yohei.yamauchi@bristol.ac.uk 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined pH 7.5 5.5 Daly JL., Simonetti B., Klein K., Chen KE., Williamson MK., Antón-Plágaro C., Shoemark DK., Simón-Gracia L., Bauer M., Hollandi R., Greber UF., Horvath P., Sessions RB., Helenius A., Hiscox JA., Teesalu T., Matthews DA., Davidson AD., Collins BM., Cullen PJ., Yamauchi Y. Virus - Publications including interactions involving viral proteins emailed authors again 17/11 to address Sandra's point. The authors have stated that:- 1] the mCherry-NRP1 b1 domain is a human derived construct. The construct corresponds to residues 274-428 of H.sapiens NRP1. 2] the mCherry-NRP2 is also human derived. 3] The SARS2 SPIKE protein "S1 region" - corresponds to residues 13-685, corresponding to the S1 protein without the signal peptide. 4] The (full length) NRP1-mCherry used in Figure 1B is Human, while the Nrp1-mCherry used in Supp. figure 1D is the Mouse protein (gift from Donatella Valdembri). bs13866@bristol.ac.uk, pete.cullen@bristol.ac.uk, yohei.yamauchi@bristol.ac.uk Accepted 2020-NOV-19 AT 09:17 GMT AT 09:17 GMT by ORCHARD Science (New York, N.Y.) 2020 Only protein-protein interactions imex curation imex curation Neuropilin-1 is a host factor for SARS-CoV-2 infection. Neuropilin-1 is a host factor for SARS-CoV-2 infection. Science (New York, N.Y.) 2020 imex curation Daly JL., Simonetti B., Klein K., Chen KE., Williamson MK., Antón-Plágaro C., Shoemark DK., Simón-Gracia L., Bauer M., Hollandi R., Greber UF., Horvath P., Sessions RB., Helenius A., Hiscox JA., Teesalu T., Matthews DA., Davidson AD., Collins BM., Cullen PJ., Yamauchi Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus emailed authors again 17/11 to address Sandra's point. The authors have stated that:- 1] the mCherry-NRP1 b1 domain is a human derived construct. The construct corresponds to residues 274-428 of H.sapiens NRP1. 2] the mCherry-NRP2 is also human derived. 3] The SARS2 SPIKE protein "S1 region" - corresponds to residues 13-685, corresponding to the S1 protein without the signal peptide. 4] The (full length) NRP1-mCherry used in Figure 1B is Human, while the Nrp1-mCherry used in Supp. figure 1D is the Mouse protein (gift from Donatella Valdembri). bs13866@bristol.ac.uk, pete.cullen@bristol.ac.uk, yohei.yamauchi@bristol.ac.uk 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Daly JL., Simonetti B., Klein K., Chen KE., Williamson MK., Antón-Plágaro C., Shoemark DK., Simón-Gracia L., Bauer M., Hollandi R., Greber UF., Horvath P., Sessions RB., Helenius A., Hiscox JA., Teesalu T., Matthews DA., Davidson AD., Collins BM., Cullen PJ., Yamauchi Y. Virus - Publications including interactions involving viral proteins emailed authors again 17/11 to address Sandra's point. Pseudovirus particles decorated with SARS-2-S were added to mCherry-NRP1 b1 previously captured on mCherry-beads. The authors have stated that:- 1] the mCherry-NRP1 b1 domain is a human derived construct. The construct corresponds to residues 274-428 of H.sapiens NRP1. 2] the mCherry-NRP2 is also human derived. 3] The SARS2 SPIKE protein "S1 region" - corresponds to residues 13-685, corresponding to the S1 protein without the signal peptide. 4] The (full length) NRP1-mCherry used in Figure 1B is Human, while the Nrp1-mCherry used in Supp. figure 1D is the Mouse protein (gift from Donatella Valdembri). bs13866@bristol.ac.uk, pete.cullen@bristol.ac.uk, yohei.yamauchi@bristol.ac.uk Accepted 2020-NOV-19 AT 09:17 GMT AT 09:17 GMT by ORCHARD Science (New York, N.Y.) 2020 Only protein-protein interactions imex curation imex curation Neuropilin-1 is a host factor for SARS-CoV-2 infection. Neuropilin-1 is a host factor for SARS-CoV-2 infection. Science (New York, N.Y.) 2020 imex curation Daly JL., Simonetti B., Klein K., Chen KE., Williamson MK., Antón-Plágaro C., Shoemark DK., Simón-Gracia L., Bauer M., Hollandi R., Greber UF., Horvath P., Sessions RB., Helenius A., Hiscox JA., Teesalu T., Matthews DA., Davidson AD., Collins BM., Cullen PJ., Yamauchi Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus emailed authors again 17/11 to address Sandra's point. The authors have stated that:- 1] the mCherry-NRP1 b1 domain is a human derived construct. The construct corresponds to residues 274-428 of H.sapiens NRP1. 2] the mCherry-NRP2 is also human derived. 3] The SARS2 SPIKE protein "S1 region" - corresponds to residues 13-685, corresponding to the S1 protein without the signal peptide. 4] The (full length) NRP1-mCherry used in Figure 1B is Human, while the Nrp1-mCherry used in Supp. figure 1D is the Mouse protein (gift from Donatella Valdembri). bs13866@bristol.ac.uk, pete.cullen@bristol.ac.uk, yohei.yamauchi@bristol.ac.uk 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Daly JL., Simonetti B., Klein K., Chen KE., Williamson MK., Antón-Plágaro C., Shoemark DK., Simón-Gracia L., Bauer M., Hollandi R., Greber UF., Horvath P., Sessions RB., Helenius A., Hiscox JA., Teesalu T., Matthews DA., Davidson AD., Collins BM., Cullen PJ., Yamauchi Y. Virus - Publications including interactions involving viral proteins emailed authors again 17/11 to address Sandra's point. The authors have stated that:- 1] the mCherry-NRP1 b1 domain is a human derived construct. The construct corresponds to residues 274-428 of H.sapiens NRP1. 2] the mCherry-NRP2 is also human derived. 3] The SARS2 SPIKE protein "S1 region" - corresponds to residues 13-685, corresponding to the S1 protein without the signal peptide. 4] The (full length) NRP1-mCherry used in Figure 1B is Human, while the Nrp1-mCherry used in Supp. figure 1D is the Mouse protein (gift from Donatella Valdembri). bs13866@bristol.ac.uk, pete.cullen@bristol.ac.uk, yohei.yamauchi@bristol.ac.uk Accepted 2020-NOV-19 AT 09:17 GMT AT 09:17 GMT by ORCHARD Science (New York, N.Y.) 2020 Only protein-protein interactions imex curation imex curation Neuropilin-1 is a host factor for SARS-CoV-2 infection. Neuropilin-1 is a host factor for SARS-CoV-2 infection. Science (New York, N.Y.) 2020 imex curation Daly JL., Simonetti B., Klein K., Chen KE., Williamson MK., Antón-Plágaro C., Shoemark DK., Simón-Gracia L., Bauer M., Hollandi R., Greber UF., Horvath P., Sessions RB., Helenius A., Hiscox JA., Teesalu T., Matthews DA., Davidson AD., Collins BM., Cullen PJ., Yamauchi Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus emailed authors again 17/11 to address Sandra's point. The authors have stated that:- 1] the mCherry-NRP1 b1 domain is a human derived construct. The construct corresponds to residues 274-428 of H.sapiens NRP1. 2] the mCherry-NRP2 is also human derived. 3] The SARS2 SPIKE protein "S1 region" - corresponds to residues 13-685, corresponding to the S1 protein without the signal peptide. 4] The (full length) NRP1-mCherry used in Figure 1B is Human, while the Nrp1-mCherry used in Supp. figure 1D is the Mouse protein (gift from Donatella Valdembri). bs13866@bristol.ac.uk, pete.cullen@bristol.ac.uk, yohei.yamauchi@bristol.ac.uk 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Daly JL., Simonetti B., Klein K., Chen KE., Williamson MK., Antón-Plágaro C., Shoemark DK., Simón-Gracia L., Bauer M., Hollandi R., Greber UF., Horvath P., Sessions RB., Helenius A., Hiscox JA., Teesalu T., Matthews DA., Davidson AD., Collins BM., Cullen PJ., Yamauchi Y. Virus - Publications including interactions involving viral proteins emailed authors again 17/11 to address Sandra's point. The authors have stated that:- 1] the mCherry-NRP1 b1 domain is a human derived construct. The construct corresponds to residues 274-428 of H.sapiens NRP1. 2] the mCherry-NRP2 is also human derived. 3] The SARS2 SPIKE protein "S1 region" - corresponds to residues 13-685, corresponding to the S1 protein without the signal peptide. 4] The (full length) NRP1-mCherry used in Figure 1B is Human, while the Nrp1-mCherry used in Supp. figure 1D is the Mouse protein (gift from Donatella Valdembri). bs13866@bristol.ac.uk, pete.cullen@bristol.ac.uk, yohei.yamauchi@bristol.ac.uk Accepted 2020-NOV-19 AT 09:17 GMT AT 09:17 GMT by ORCHARD Science (New York, N.Y.) 2020 Only protein-protein interactions imex curation imex curation eg00229 EG00229 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 nrp2_human Neuropilin-2 NRP2 VEGF165R2 Vascular endothelial cell growth factor 165 receptor 2 protein protein human Homo sapiens Human MDMFPLTWVFLALYFSRHQVRGQPDPPCGGRLNSKDAGYITSPGYPQDYPSHQNCEWIVYAPEPNQKIVLNFNPHFEIEKHDCKYDFIEIRDGDSESADLLGKHCGNIAPPTIISSGSMLYIKFTSDYARQGAGFSLRYEIFKTGSEDCSKNFTSPNGTIESPGFPEKYPHNLDCTFTILAKPKMEIILQFLIFDLEHDPLQVGEGDCKYDWLDIWDGIPHVGPLIGKYCGTKTPSELRSSTGILSLTFHTDMAVAKDGFSARYYLVHQEPLENFQCNVPLGMESGRIANEQISASSTYSDGRWTPQQSRLHGDDNGWTPNLDSNKEYLQVDLRFLTMLTAIATQGAISRETQNGYYVKSYKLEVSTNGEDWMVYRHGKNHKVFQANNDATEVVLNKLHAPLLTRFVRIRPQTWHSGIALRLELFGCRVTDAPCSNMLGMLSGLIADSQISASSTQEYLWSPSAARLVSSRSGWFPRIPQAQPGEEWLQVDLGTPKTVKGVIIQGARGGDSITAVEARAFVRKFKVSYSLNGKDWEYIQDPRTQQPKLFEGNMHYDTPDIRRFDPIPAQYVRVYPERWSPAGIGMRLEVLGCDWTDSKPTVETLGPTVKSEETTTPYPTEEEATECGENCSFEDDKDLQLPSGFNCNFDFLEEPCGWMYDHAKWLRTTWASSSSPNDRTFPDDRNFLRLQSDSQREGQYARLISPPVHLPRSPVCMEFQYQATGGRGVALQVVREASQESKLLWVIREDQGGEWKHGRIILPSYDMEYQIVFEGVIGKGRSGEIAIDDIRISTDVPLENCMEPISAFAGENFKVDIPEIHEREGYEDEIDDEYEVDWSNSSSATSGSGAPSTDKEKSWLYTLDPILITIIAMSSLGVLLGATCAGLLLYCTCSYSGLSSRSCTTLENYNFELYDGLKHKVKMNHQKCCSEA DIP protein O60462 original sequence version: 133 DIP protein O60462 original sequence version: 142 270CBAE69A0A797C spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT nrp1_mouse Neuropilin-1 A5 protein Nrp1 Nrp protein protein mouse Mus musculus Mouse MERGLPLLCATLALALALAGAFRSDKCGGTIKIENPGYLTSPGYPHSYHPSEKCEWLIQAPEPYQRIMINFNPHFDLEDRDCKYDYVEVIDGENEGGRLWGKFCGKIAPSPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTAPTGVIKSPGFPEKYPNSLECTYIIFAPKMSEIILEFESFDLEQDSNPPGGMFCRYDRLEIWDGFPEVGPHIGRYCGQKTPGRIRSSSGVLSMVFYTDSAIAKEGFSANYSVLQSSISEDFKCMEALGMESGEIHSDQITASSQYGTNWSVERSRLNYPENGWTPGEDSYKEWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYRVDISSNGEDWISLKEGNKAIIFQGNTNPTDVVLGVFSKPLITRFVRIKPVSWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITASNQADRNWMPENIRLVTSRTGWALPPSPHPYTNEWLQVDLGDEKIVRGVIIQGGKHRENKVFMRKFKIAYSNNGSDWKTIMDDSKRKAKSFEGNNNYDTPELRTFSPLSTRFIRIYPERATHSGLGLRMELLGCEVEAPTAGPTTPNGNPVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTIIDSTIQSEFPTYGFNCEFGWGSHKTFCHWEHDSHAQLRWSVLTSKTGPIQDHTGDGNFIYSQADENQKGKVARLVSPVVYSQSSAHCMTFWYHMSGSHVGTLRVKLRYQKPEEYDQLVWMVVGHQGDHWKEGRVLLHKSLKLYQVIFEGEIGKGNLGGIAVDDISINNHISQEDCAKPTDLDKKNTEIKIDETGSTPGYEGEGEGDKNISRKPGNVLKTLDPILITIIAMSALGVLLGAVCGVVLYCACWHNGMSERNLSALENYNFELVDGVKLKKDKLNPQSNYSEA DIP protein P97333 original sequence version: 97 DIP protein P97333 original sequence version: 116 DIP protein P97333 original sequence version: 123 23209818C5C42137 nrp1_human Neuropilin-1 Vascular endothelial cell growth factor 165 receptor NRP1 NRP VEGF165R protein protein human Homo sapiens Human MERGLPLLCAVLALVLAPAGAFRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFVPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSEFPTYGFNCEFGWGSHKTFCHWEHDNHVQLKWSVLTSKTGPIQDHTGDGNFIYSQADENQKGKVARLVSPVVYSQNSAHCMTFWYHMSGSHVGTLRVKLRYQKPEEYDQLVWMAIGHQGDHWKEGRVLLHKSLKLYQVIFEGEIGKGNLGGIAVDDISINNHISQEDCAKPADLDKKNPEIKIDETGSTPGYEGEGEGDKNISRKPGNVLKTLDPILITIIAMSALGVLLGAVCGVVLYCACWHNGMSERNLSALENYNFELVDGVKLKKDKLNTQSTYSEA DIP protein O14786 original sequence version: 114 1EAC2FA6C8FD6A0B s-nrp1-1 1 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association 1A nrp1-s-1 6 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. figure 1D nrp1-s-2 2 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0DTC2:p.Arg685Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role neutral component neutral component purified purified b1 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3 2A, Table S1 nrp1-s-3 2 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0DTC2:p.Arg685Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role neutral component neutral component purified purified b1 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 4 2A, Table S1 nrp1-s-4 7 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role neutral component neutral component purified purified b1 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 4 Table S3 nrp1-s-5 6 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0DTC2:p.Arg685Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R D 13 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined b1 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2C, 3C, Supp. figure 4D Inhibitor EG00229 (an NRP1 receptor antagonist) (CHEBI:166820) nrp1-s-6 6 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined b1 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O14786:p.Thr316Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T R physical association physical association 2D nrp1-s-7 2 8 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component purified purified b1 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3 3E, Table S2 eg00229-nrp1-1 2 8 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component purified purified b1 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 4 3E, Table S2 nrp1-s-8 2 8 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role neutral component neutral component purified purified b1 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 4 3E, Table S2 I have not entered any Kd values -as far as I am aware Kd calculations are based on 2 interactors, not 3, so I do not know what the values refer to here. It is usual to give an IC50 value with inhibitors. nrp1-s-9 6 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. figure 1D/E s-nrp1-2 6 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined b1 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Supp. figure 3B nrp1-s-10 5 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 13 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined b1 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O14786:p.Thr316Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T R Sun Dec 13 19:41:35 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'O14786:p. Thr316Arg' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Supp. Figure 3F nrp1-s-11 6 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1B NRP1 immunoprecipitated the S1 protein, and deletion of the CendR motif reduced this association.