IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SARS-CoV-2 Nsp1 suppresses host but not viral translation through a bipartite mechanism. SARS-CoV-2 Nsp1 suppresses host but not viral translation through a bipartite mechanism. bioRxiv : the preprint server for biology 2020 imex curation Ming Shi Sr., Longfei Wang, Pietro Fontana, Setu Vora, Ying Zhang, Tian-Min Fu, Judy Lieberman, Hao Wu, Shi M., Wang L., Fontana P., Vora S., Zhang Y., Fu TM., Lieberman J., Wu H., Shi M., Wang L., Fontana P., Vora S., Zhang Y., Fu TM., Lieberman J., Wu H. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus This entry has been curated from a pre-print and has not been peer-reviewed. If needed, the data in this entry will be amended once the manuscript has gone through peer-review shiming@hit.edu.cn,longfei_wang@hms.harvard.edu,pietro.fontana@childrens.harvard.edu,setu.vora@childrens.harvard.edu,ying.zhang@childrens.harvard.edu,ying.zhang@childrens.harvard.edu,judy.lieberman@childrens.harvard.edu,hao.wu@childrens.harvard.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ming Shi Sr., Longfei Wang, Pietro Fontana, Setu Vora, Ying Zhang, Tian-Min Fu, Judy Lieberman, Hao Wu, Shi M., Wang L., Fontana P., Vora S., Zhang Y., Fu TM., Lieberman J., Wu H., Shi M., Wang L., Fontana P., Vora S., Zhang Y., Fu TM., Lieberman J., Wu H. Virus - Publications including interactions involving viral proteins This entry has been curated from a pre-print and has not been peer-reviewed. If needed, the data in this entry will be amended once the manuscript has gone through peer-review shiming@hit.edu.cn,longfei_wang@hms.harvard.edu,pietro.fontana@childrens.harvard.edu,setu.vora@childrens.harvard.edu,ying.zhang@childrens.harvard.edu,ying.zhang@childrens.harvard.edu,judy.lieberman@childrens.harvard.edu,hao.wu@childrens.harvard.edu Accepted 2020-DEC-16 AT 09:32 GMT AT 09:32 GMT by MGT bioRxiv : the preprint server for biology 2020 Only protein-protein interactions imex curation imex curation SARS-CoV-2 Nsp1 suppresses host but not viral translation through a bipartite mechanism. SARS-CoV-2 Nsp1 suppresses host but not viral translation through a bipartite mechanism. bioRxiv : the preprint server for biology 2020 imex curation Ming Shi Sr., Longfei Wang, Pietro Fontana, Setu Vora, Ying Zhang, Tian-Min Fu, Judy Lieberman, Hao Wu, Shi M., Wang L., Fontana P., Vora S., Zhang Y., Fu TM., Lieberman J., Wu H., Shi M., Wang L., Fontana P., Vora S., Zhang Y., Fu TM., Lieberman J., Wu H. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus This entry has been curated from a pre-print and has not been peer-reviewed. If needed, the data in this entry will be amended once the manuscript has gone through peer-review shiming@hit.edu.cn,longfei_wang@hms.harvard.edu,pietro.fontana@childrens.harvard.edu,setu.vora@childrens.harvard.edu,ying.zhang@childrens.harvard.edu,ying.zhang@childrens.harvard.edu,judy.lieberman@childrens.harvard.edu,hao.wu@childrens.harvard.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Ming Shi Sr., Longfei Wang, Pietro Fontana, Setu Vora, Ying Zhang, Tian-Min Fu, Judy Lieberman, Hao Wu, Shi M., Wang L., Fontana P., Vora S., Zhang Y., Fu TM., Lieberman J., Wu H., Shi M., Wang L., Fontana P., Vora S., Zhang Y., Fu TM., Lieberman J., Wu H. Virus - Publications including interactions involving viral proteins This entry has been curated from a pre-print and has not been peer-reviewed. If needed, the data in this entry will be amended once the manuscript has gone through peer-review CPX-5223 complex has recently been updated with addition of RACK1, that was missing previously. The current version can be found at https://www.ebi.ac.uk/intact/editor/complex/EBI-26452941 (currently review under progress) shiming@hit.edu.cn,longfei_wang@hms.harvard.edu,pietro.fontana@childrens.harvard.edu,setu.vora@childrens.harvard.edu,ying.zhang@childrens.harvard.edu,ying.zhang@childrens.harvard.edu,judy.lieberman@childrens.harvard.edu,hao.wu@childrens.harvard.edu Accepted 2020-DEC-16 AT 09:32 GMT AT 09:32 GMT by MGT bioRxiv : the preprint server for biology 2020 Only protein-protein interactions imex curation imex curation Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method SARS-CoV-2 Nsp1 suppresses host but not viral translation through a bipartite mechanism. SARS-CoV-2 Nsp1 suppresses host but not viral translation through a bipartite mechanism. bioRxiv : the preprint server for biology 2020 imex curation Ming Shi Sr., Longfei Wang, Pietro Fontana, Setu Vora, Ying Zhang, Tian-Min Fu, Judy Lieberman, Hao Wu, Shi M., Wang L., Fontana P., Vora S., Zhang Y., Fu TM., Lieberman J., Wu H., Shi M., Wang L., Fontana P., Vora S., Zhang Y., Fu TM., Lieberman J., Wu H. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus This entry has been curated from a pre-print and has not been peer-reviewed. If needed, the data in this entry will be amended once the manuscript has gone through peer-review shiming@hit.edu.cn,longfei_wang@hms.harvard.edu,pietro.fontana@childrens.harvard.edu,setu.vora@childrens.harvard.edu,ying.zhang@childrens.harvard.edu,ying.zhang@childrens.harvard.edu,judy.lieberman@childrens.harvard.edu,hao.wu@childrens.harvard.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Ming Shi Sr., Longfei Wang, Pietro Fontana, Setu Vora, Ying Zhang, Tian-Min Fu, Judy Lieberman, Hao Wu, Shi M., Wang L., Fontana P., Vora S., Zhang Y., Fu TM., Lieberman J., Wu H., Shi M., Wang L., Fontana P., Vora S., Zhang Y., Fu TM., Lieberman J., Wu H. Virus - Publications including interactions involving viral proteins This entry has been curated from a pre-print and has not been peer-reviewed. If needed, the data in this entry will be amended once the manuscript has gone through peer-review shiming@hit.edu.cn,longfei_wang@hms.harvard.edu,pietro.fontana@childrens.harvard.edu,setu.vora@childrens.harvard.edu,ying.zhang@childrens.harvard.edu,ying.zhang@childrens.harvard.edu,judy.lieberman@childrens.harvard.edu,hao.wu@childrens.harvard.edu Accepted 2020-DEC-16 AT 09:32 GMT AT 09:32 GMT by MGT bioRxiv : the preprint server for biology 2020 Only protein-protein interactions imex curation imex curation rs11_human 40S ribosomal protein S11 RPS11 Small ribosomal subunit protein uS17 protein protein human Homo sapiens Human MADIQTERAYQKQPTIFQNKKRVLLGETGKEKLPRYYKNIGLGFKTPKEAIEGTYIDKKCPFTGNVSIRGRILSGVVTKMKMQRTIVIRRDYLHYIRKYNRFEKRHKNMSVHLSPCFRDVQIGDIVTVGECRPLSKTVRFNVLKVTKAAGTKKQFQKF DIP protein P62280 original sequence version: null 9FB75DC1D99614B0 rs10_human 40S ribosomal protein S10 RPS10 Small ribosomal subunit protein eS10 protein protein human Homo sapiens Human MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ 64106DFCD97AABA3 rs29_human 40S ribosomal protein S29 RPS29 Small ribosomal subunit protein uS14 protein protein human Homo sapiens Human MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIGFIKLD 41325122B493EFF9 rs24_human 40S ribosomal protein S24 RPS24 Small ribosomal subunit protein eS24 protein protein human Homo sapiens Human MNDTVTIRTRKFMTNRLLQRKQMVIDVLHPGKATVPKTEIREKLAKMYKTTPDVIFVFGFRTHFGGGKTTGFGMIYDSLDYAKKNEPKHRLARHGLYEKKKTSRKQRKERKNRMKKVRGTAKANVGAGKKPKE CE9065C0764D60A6 rs14_human 40S ribosomal protein S14 RPS14 Small ribosomal subunit protein uS11 PRO2640 protein protein human Homo sapiens Human MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDSTRRKGGRRGRRL 4F4387F9FADAC278 rs12_human 40S ribosomal protein S12 RPS12 Small ribosomal subunit protein eS12 protein protein human Homo sapiens Human MAEEGIAAGGVMDVNTALQEVLKTALIHDGLARGIREAAKALDKRQAHLCVLASNCDEPMYVKLVEALCAEHQINLIKVDDNKKLGEWVGLCKIDREGKPRKVVGCSCVVVKDYGKESQAKDVIEEYFKCKK 45AD1274D55FFA25 p0dtd1-pro_0000449619 Host translation inhibitor nsp1 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGG 1 180 rssa_human 40S ribosomal protein SA RPSA LAMBR LAMR1 Laminin receptor 1 37/67 kDa laminin receptor Laminin-binding protein precursor p40 Colon carcinoma laminin-binding protein NEM/1CHD4 Multidrug resistance-associated protein MGr1-Ag 37 kDa laminin receptor precursor 67 kDa laminin receptor Small ribosomal subunit protein uS2 protein protein human Homo sapiens Human MSGALDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVSVISSRNTGQRAVLKFAAATGATPIAGRFTPGTFTNQIQAAFREPRLLVVTDPRADHQPLTEASYVNLPTIALCNTDSPLRYVDIAIPCNNKGAHSVGLMWWMLAREVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPAPEFTATQPEVADWSEGVQVPSVPIQQFPTEDWSAQPATEDWSAAPTAQATEWVGATTDWS DIP protein P08865 original sequence version: 163 C68DDB16B759E79E rs5_human 40S ribosomal protein S5 RPS5 Small ribosomal subunit protein uS7 protein protein human Homo sapiens Human MTEWETAAPAVAETPDIKLFGKWSTDDVQINDISLQDYIAVKEKYAKYLPHSAGRYAAKRFRKAQCPIVERLTNSMMMHGRNNGKKLMTVRIVKHAFEIIHLLTGENPLQVLVNAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR DFE2FD5AAFCFD894 rs3a_human 40S ribosomal protein S3a RPS3A MFTL FTE1 v-fos transformation effector protein Small ribosomal subunit protein eS1 protein protein human Homo sapiens Human MAVGKNKRLTKGGKKGAKKKVVDPFSKKDWYDVKAPAMFNIRNIGKTLVTRTQGTKIASDGLKGRVFEVSLADLQNDEVAFRKFKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTMIEAHVDVKTTDGYLLRLFCVGFTKKRNNQIRKTSYAQHQQVRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQSIYPLHDVFVRKVKMLKKPKFELGKLMELHGEGSSSGKATGDETGAKVERADGYEPPVQESV DIP protein P61247 original sequence version: 37 000037AE195F7A9D rs2_human 40S ribosomal protein S2 RPS2 RPS4 40S ribosomal protein S4 Protein LLRep3 Small ribosomal subunit protein uS5 protein protein human Homo sapiens Human MADDAGAAGGPGGPGGPGMGNRGGFRGGFGSGIRGRGRGRGRGRGRGRGARGGKAEDKEWMPVTKLGRLVKDMKIKSLEEIYLFSLPIKESEIIDFFLGASLKDEVLKIMPVQKQTRAGQRTRFKAFVAIGDYNGHVGLGVKCSKEVATAIRGAIILAKLSIVPVRRGYWGNKIGKPHTVPCKVTGRCGSVLVRLIPAPRGTGIVSAPVPKKLLMMAGIDDCYTSARGCTATLGNFAKATFDAISKTYSYLTPDLWKETVFTKSPYQEFTDHLVKTHTRVSVQRTQAPAVATT 66C0DB7ED393B036 rs4x_rs4y1_rs4y2_human RPS4X, RPS4Y1 and RPS4Y2 molecule set molecule set human Homo sapiens Human P22090,P62701,Q8TD47 rs26_human 40S ribosomal protein S26 RPS26 Small ribosomal subunit protein eS26 protein protein human Homo sapiens Human MTKKRRNNGRAKKGRGHVQPIRCTNCARCVPKDKAIKKFVIRNIVEAAAVRDISEASVFDAYVLPKLYVKLHYCVSCAIHSKVVRNRSREARKDRTPPPRFRPAGAAPRPPPKPM F60DF98F8900D968 5'utr_sars-cov 2 5'UTR_SARS-CoV 2 dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV ATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAG rrna18s human ribosomal RNA 18s human 5 rrna ribosomal rna rRNA rrna human Homo sapiens Human UACCUGGUUGAUCCUGCCAGUAGCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUAAGUACGCACGGCCGGUACAGUGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUGGUUCCUUUGGUCGCUCGCUCCUCUCCUACUUGGAUAACUGUGGUAAUUCUAGAGCUAAUACAUGCCGACGGGCGCUGACCCCCUUCGCGGGGGGGAUGCGUGCAUUUAUCAGAUCAAAACCAACCCGGUCAGCCCCUCUCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCUUUGGUGACUCUAGAUAACCUCGGGCCGAUCGCACGCCCCCCGUGGCGGCGACGACCCAUUCGAACGUCUGCCCUAUCAACUUUCGAUGGUAGUCGCCGUGCCUACCAUGGUGACCACGGGUGACGGGGAAUCAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAAACGGCUACCACAUCCAAGGAAGGCAGCAGGCGCGCAAAUUACCCACUCCCGACCCGGGGAGGUAGUGACGAAAAAUAACAAUACAGGACUCUUUCGAGGCCCUGUAAUUGGAAUGAGUCCACUUUAAAUCCUUUAACGAGGAUCCAUUGGAGGGCAAGUCUGGUGCCAGCAGCCGCGGUAAUUCCAGCUCCAAUAGCGUAUAUUAAAGUUGCUGCAGUUAAAAAGCUCGUAGUUGGAUCUUGGGAGCGGGCGGGCGGUCCGCCGCGAGGCGAGCCACCGCCCGUCCCCGCCCCUUGCCUCUCGGCGCCCCCUCGAUGCUCUUAGCUGAGUGUCCCGCGGGGCCCGAAGCGUUUACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCCCGAGCCGCCUGGAUACCGCAGCUAGGAAUAAUGGAAUAGGACCGCGGUUCUAUUUUGUUGGUUUUCGGAACUGAGGCCAUGAUUAAGAGGGACGGCCGGGGGCAUUCGUAUUGCGCCGCUAGAGGUGAAAUUCUUGGACCGGCGCAAGACGGACCAGAGCGAAAGCAUUUGCCAAGAAUGUUUUCAUUAAUCAAGAACGAAAGUCGGAGGUUCGAAGACGAUCAGAUACCGUCGUAGUUCCGACCAUAAACGAUGCCGACCGGCGAUGCGGCGGCGUUAUUCCCAUGACCCGCCGGGCAGCUUCCGGGAAACCAAAGUCUUUGGGUUCCGGGGGGAGUAUGGUUGCAAAGCUGAAACUUAAAGGAAUUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGGAAACCUCACCCGGCCCGGACACGGACAGGAUUGACAGAUUGAUAGCUCUUUCUCGAUUCCGUGGGUGGUGGUGCAUGGCCGUUCUUAGUUGGUGGAGCGAUUUGUCUGGUUAAUUCCGAUAACGAACGAGACUCUGGCAUGCUAACUAGUUACGCGACCCCCGAGCGGUCGGCGUCCCCCAACUUCUUAGAGGGACAAGUGGCGUUCAGCCACCCGAGAUUGAGCAAUAACAGGUCUGUGAUGCCCUUAGAUGUCCGGGGCUGCACGCGCGCUACACUGACUGGCUCAGCGUGUGCCUACCCUACGCCGGCAGGCGCGGGUAACCCGUUGAACCCCAUUCGUGAUGGGGAUCGGGGAUUGCAAUUAUUCCCCAUGAACGAGGAAUUCCCAGUAAGUGCGGGUCAUAAGCUUGCGUUGAUUAAGUCCCUGCCCUUUGUACACACCGCCCGUCGCUACUACCGAUUGGAUGGUUUAGUGAGGCCCUCGGAUCGGCCCCGCCGGGGUCGGCCCACGGCCCUGGCGGAGCGCUGAGAAGACGGUCGAACUUGACUAUCUAGAGGAAGUAAAAGUCGUAACAAGGUUUCCGUAGGUGAACCUGCGGAAGGAUCAUUA rs27_rs27l_human RPS27 and RPS27L molecule set molecule set human Homo sapiens Human Q71UM5,P42677 rs3_human 40S ribosomal protein S3 RPS3 Small ribosomal subunit protein uS3 OK/SW-cl.26 protein protein human Homo sapiens Human MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSIVEPKDEILPTTPISEQKGGKPEPPAMPQPVPTA DIP protein P23396 original sequence version: 174 6ECBB34A8EE04AAF rs18_human 40S ribosomal protein S18 RPS18 D6S218E Ke-3 Small ribosomal subunit protein uS13 protein protein human Homo sapiens Human MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK DIP protein P62269 original sequence version: 125 4DAF0662C3F37F22 rs19_human 40S ribosomal protein S19 RPS19 Small ribosomal subunit protein eS19 protein protein human Homo sapiens Human MPGVTVKDVNQQEFVRALAAFLKKSGKLKVPEWVDTVKLAKHKELAPYDENWFYTRAASTARHLYLRGGAGVGSMTKIYGGRQRNGVMPSHFSRGSKSVARRVLQALEGLKMVEKDQDGGRKLTPQGQRDLDRIAGQVAAANKKH 181F2DB898E56E41 rs23_human 40S ribosomal protein S23 RPS23 Small ribosomal subunit protein uS12 protein protein human Homo sapiens Human MGKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRS 8417A48B8CF0A8E5 rack1_human Receptor of activated protein C kinase 1 Small ribosomal subunit protein RACK1 HLC7 PIG21 RACK1 Cell proliferation-inducing gene 21 protein GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 Human lung cancer oncogene 7 protein Receptor for activated C kinase protein protein human Homo sapiens Human MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR DIP protein P63244 original sequence version: 71 257F91E369ED2044 rs25_human 40S ribosomal protein S25 RPS25 Small ribosomal subunit protein eS25 protein protein human Homo sapiens Human MPPKDDKKKKDAGKSAKKDKDPVNKSGGKAKKKKWSKGKVRDKLNNLVLFDKATYDKLCKEVPNYKLITPAVVSERLKIRGSLARAALQELLSKGLIKLVSKHRAQVIYTRNTKGGDAPAAGEDA B8E05F04FCF2F1A9 rs27a_human Ubiquitin-40S ribosomal protein S27a RPS27A UBA80 UBCEP1 Ubiquitin carboxyl extension protein 80 protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAKKRKKKSYTTPKKNKHKRKKVKLAVLKYYKVDENGKISRLRRECPSDECGAGVFMASHFDRHYCGKCCLTYCFNKPEDK 617BC63DF3A904F7 rs16_human 40S ribosomal protein S16 RPS16 Small ribosomal subunit protein uS9 protein protein human Homo sapiens Human MPSKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR DIP protein P62249 original sequence version: 115 519BCFB91BB68A15 rs28_human 40S ribosomal protein S28 RPS28 Small ribosomal subunit protein eS28 protein protein human Homo sapiens Human MDTSRVQPIKLARVTKVLGRTGSQGQCTQVRVEFMDDTSRSIIRNVKGPVREGDVLTLLESEREARRLR 49902FE9376EB74F rs15_human 40S ribosomal protein S15 RIG protein RPS15 RIG Small ribosomal subunit protein uS19 protein protein human Homo sapiens Human MAEVEQKKKRTFRKFTYRGVDLDQLLDMSYEQLMQLYSARQRRRLNRGLRRKQHSLLKRLRKAKKEAPPMEKPEVVKTHLRDMIILPEMVGSMVGVYNGKTFNQVEIKPEMIGHYLGEFSITYKPVKHGRPGIGATHSSRFIPLK CA2C682302C7B387 p62945-pro_0000198055 60S ribosomal protein L41 RPL41 HG12 Large ribosomal subunit protein eL41 protein protein human Homo sapiens Human MRAKWRKKRMRRLKRKRRKMRQRSK 1 25 9AD629C69ECC039E rs21_human 40S ribosomal protein S21 RPS21 Small ribosomal subunit protein eS21 protein protein human Homo sapiens Human MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKADGIVSKNF 6794AC1292A06BD3 rs20_human 40S ribosomal protein S20 RPS20 Small ribosomal subunit protein uS10 protein protein human Homo sapiens Human MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA DIP protein P60866 original sequence version: 112 8802DF7894ADDF94 rs6_human 40S ribosomal protein S6 RPS6 Phosphoprotein NP33 Small ribosomal subunit protein eS6 OK/SW-cl.2 protein protein human Homo sapiens Human MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK DIP protein P62753 original sequence version: 72 DIP protein P62753 original sequence version: 68 DIP protein P62753 original sequence version: 123 A61E435884E636AE rs15a_human 40S ribosomal protein S15a RPS15A Small ribosomal subunit protein uS8 OK/SW-cl.82 protein protein human Homo sapiens Human MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF D4AC1E8864E3A184 rs9_human 40S ribosomal protein S9 RPS9 Small ribosomal subunit protein uS4 protein protein human Homo sapiens Human MPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAARELLTLDEKDPRRLFEGNALLRRLVRIGVLDEGKMKLDYILGLKIEDFLERRLQTQVFKLGLAKSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFSLRSPYGGGRPGRVKRKNAKKGQGGAGAGDDEEED E9CE3CBD59524F81 rs8_human 40S ribosomal protein S8 RPS8 Small ribosomal subunit protein eS8 OK/SW-cl.83 protein protein human Homo sapiens Human MGISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGGNKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNCIVLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDERKKNAKISSLLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRKIKARKGK 407FDF59171F7422 rs7_human 40S ribosomal protein S7 RPS7 Small ribosomal subunit protein eS7 protein protein human Homo sapiens Human MFSSSAKIVKPNGEKPDEFESGISQALLELEMNSDLKAQLRELNITAAKEIEVGGGRKAIIIFVPVPQLKSFQKIQVRLVRELEKKFSGKHVVFIAQRRILPKPTRKSRTKNKQKRPRSRTLTAVHDAILEDLVFPSEIVGKRIRVKLDGSRLIKVHLDKAQQNNVEHKVETFSGVYKKLTGKDVNFEFPEFQL E21089929CC061E9 rs17_human 40S ribosomal protein S17 RPS17 Small ribosomal subunit protein eS17 RPS17L protein protein human Homo sapiens Human MGRVRTKTVKKAARVIIEKYYTRLGNDFHTNKRVCEEIAIIPSKKLRNKIAGYVTHLMKRIQRGPVRGISIKLQEEERERRDNYVPEVSALDQEIIEVDPDTKEMLKLLDFGSLSNLQVTQPTVGMNFKTPRGPV 299AD605C5401325 rs13_human 40S ribosomal protein S13 RPS13 Small ribosomal subunit protein uS15 protein protein human Homo sapiens Human MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA 23F94D38F87B8D53 rs30_human 40S ribosomal protein S30 FAU Small ribosomal subunit protein eS30 protein protein human Homo sapiens Human KVHGSLARAGKVRGQTPKVAKQEKKKKKTGRAKRRMQYNRRFVNVVPTFGKKKGPNANS 012AC1FB555B01A4 40s_human-rep-1 2 19 unspecified role unspecified role neutral component neutral component purified purified human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 31 unspecified role unspecified role neutral component neutral component purified purified human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 11 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 49 unspecified role unspecified role neutral component neutral component purified purified human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Fig. 1e PDBe also lists RPL41 (UniprotKB P62945) as part of 40S ribosomal complex; but it appears to be part of 60S complex. So RPL41 has been added as a separate participant. There is lack of consistency on the host organism of purified human ribosomes - the authors mention that ribosomes were purified from Expi293 cells, but ref. 14 and possibly the figure legend states HeLa as the source. 5'utr_sars_cov 2-rep-1 4 11 immunostaining immunostaining tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 18 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed 5-prime UTR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mScarlet tag fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Figs. 3b/e, 4c-d 40s_human-rep-3 1 19 unspecified role unspecified role neutral component neutral component purified purified human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 31 unspecified role unspecified role neutral component neutral component purified purified human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 11 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 49 unspecified role unspecified role neutral component neutral component purified purified human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Fig. 1C PDBe also lists RPL41 (UniprotKB P62945) as part of 40S ribosomal complex; but it appears to be part of 60S complex. So RPL41 has been added as a separate participant. There is lack of consistency on the host organism of purified human ribosomes - the authors mention that ribosomes were purified from Expi293 cells, but ref. 14 and possibly the figure legend states HeLa as the source. 40s_human-1 SSU 40S cytosolic small ribosomal subunit FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29 25 unspecified role unspecified role 12 unspecified role unspecified role 36 unspecified role unspecified role 37 unspecified role unspecified role 38 unspecified role unspecified role 34 unspecified role unspecified role 13 unspecified role unspecified role RPS25 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS28 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS14 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 unspecified role unspecified role 14 unspecified role unspecified role RPS14 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role FAU binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS20 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS10 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role 29 unspecified role unspecified role RPS5 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 27 unspecified role unspecified role 20 unspecified role unspecified role 17 unspecified role unspecified role 26 unspecified role unspecified role RPS19 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS5 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role 24 unspecified role unspecified role 32 unspecified role unspecified role 33 unspecified role unspecified role RPS3 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role 23 unspecified role unspecified role RPS16 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS25 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role 39 unspecified role unspecified role 28 unspecified role unspecified role RPS19 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 35 unspecified role unspecified role 30 unspecified role unspecified role 9 unspecified role unspecified role RPS5 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS3A binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 40 unspecified role unspecified role 10 unspecified role unspecified role RPS10 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role 6 unspecified role unspecified role RPS3 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS12 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 41 unspecified role unspecified role RPS3 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 19 unspecified role unspecified role physical association physical association human Homo sapiens Human stable complex stable complex biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA. The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs. Accepted 2020-NOV-23 by ORCHARD