IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. ACS infectious diseases 2020 imex curation Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus andyhsu@ntu.edu.tw 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins andyhsu@ntu.edu.tw Accepted 2020-DEC-15 AT 17:17 GMT AT 17:17 GMT by MGT ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. ACS infectious diseases 2020 imex curation Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus andyhsu@ntu.edu.tw 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins andyhsu@ntu.edu.tw Accepted 2020-DEC-15 AT 17:17 GMT AT 17:17 GMT by MGT ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. ACS infectious diseases 2020 imex curation Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus andyhsu@ntu.edu.tw 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro thermal shift thermal shift binding thermal shift predetermined predetermined participant predetermined Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins DSF Assay (Differential scanning fluorimetry): SYPRO Orange binds nonspecifically to hydrophobic surfaces, and water strongly quenches its fluorescence. When the protein unfolds, the exposed hydrophobic surfaces bind the dye, resulting in an increase in fluorescence. andyhsu@ntu.edu.tw Accepted 2020-DEC-15 AT 17:17 GMT AT 17:17 GMT by MGT ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. ACS infectious diseases 2020 imex curation Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus andyhsu@ntu.edu.tw 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay antibody detection identification by antibody antibody detection Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins andyhsu@ntu.edu.tw Accepted 2020-DEC-15 AT 17:17 GMT AT 17:17 GMT by MGT ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. ACS infectious diseases 2020 imex curation Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus andyhsu@ntu.edu.tw 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro thermal shift thermal shift binding thermal shift predetermined predetermined participant predetermined Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins Thermal transition profiles of the protein with or without ADPR were monitored by using Circular Dichroism. andyhsu@ntu.edu.tw Accepted 2020-DEC-15 AT 17:17 GMT AT 17:17 GMT by MGT ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. ACS infectious diseases 2020 imex curation Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus andyhsu@ntu.edu.tw 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro thermal shift thermal shift binding thermal shift predetermined predetermined participant predetermined Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins Thermal shift of SARS-CoV-2 macro domain on binding with ADPR, ADP, and AMP binding was detected by intrinsic fluorescence from aromatic residues. andyhsu@ntu.edu.tw Accepted 2020-DEC-15 AT 17:17 GMT AT 17:17 GMT by MGT ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. ACS infectious diseases 2020 imex curation Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus andyhsu@ntu.edu.tw 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro de-adp-ribosylation assay de-ADP-ribosylation assay tag visualisation tag visualisation Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins PARP10 has been auto-mono-ADPribosylated with biotin-labelled ADP andyhsu@ntu.edu.tw Accepted 2020-DEC-15 AT 17:17 GMT AT 17:17 GMT by MGT ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. Structural, biophysical and biochemical elucidation of the SARS-CoV-2 nonstructural protein 3 macro domain. ACS infectious diseases 2020 imex curation Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus andyhsu@ntu.edu.tw 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro adp ribosylase adp ribosylase assay adp ribosylase tag visualisation tag visualisation Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins andyhsu@ntu.edu.tw Accepted 2020-DEC-15 AT 17:17 GMT AT 17:17 GMT by MGT ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation poa1_yeast ADP-ribose 1''-phosphate phosphatase POA1 [Protein ADP-ribosylglutamate] hydrolase YBR0304 YBR022W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNITYVKGNILKPKSYARILIHSCNCNGSWGGGIAYQLALRYPKAEKDYVEVCEKYGSNLLGKCILLPSYENSDLLICCLFTSSFGGSSHGEKQSILNYTKLALDKLKTFREAKDKTRTSEDSIGDYLNGHIKYPIGEYKLEMPQINSGIFGVPWKETERVLEEFSGDMSFTVYQL 006E55E9E598887A p0dtc1-pro_0000449637 Papain-like protease nsp3 ORF1a polyprotein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV APTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKVPTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLINTLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNSVPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWLMWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRVECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVECLKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALIWNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGG 819 2763 FFFD2D956385E317 adp adenosine 5'-(trihydrogen diphosphate) (ADP) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) nad+ nicotinamide adenine dinucleotide - oxidised form small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) par10_human Protein mono-ADP-ribosyltransferase PARP10 Poly [ADP-ribose] polymerase 10 ADP-ribosyltransferase diphtheria toxin-like 10 PARP10 protein protein human Homo sapiens Human MVAMAEAEAGVAVEVRGLPPAVPDELLTLYFENRRRSGGGPVLSWQRLGCGGVLTFREPADAERVLAQADHELHGAQLSLRPAPPRAPARLLLQGLPPGTTPQRLEQHVQALLRASGLPVQPCCALASPRPDRALVQLPKPLSEADVRVLEEQAQNLGLEGTLVSLARVPQARAVRVVGDGASVDLLLLELYLENERRSGGGPLEDLQRLPGPLGTVASFQQWQVAERVLQQEHRLQGSELSLVPHYDILEPEELAENTSGGDHPSTQGPRATKHALLRTGGLVTALQGAGTVTMGSGEEPGQSGASLRTGPMVQGRGIMTTGSGQEPGQSGTSLRTGPMGSLGQAEQVSSMPMGSLEHEGLVSLRPVGLQEQEGPMSLGPVGSAGPVETSKGLLGQEGLVEIAMDSPEQEGLVGPMEITMGSLEKAGPVSPGCVKLAGQEGLVEMVLLMEPGAMRFLQLYHEDLLAGLGDVALLPLEGPDMTGFRLCGAQASCQAAEEFLRSLLGSISCHVLCLEHPGSARFLLGPEGQHLLQGLEAQFQCVFGTERLATATLDTGLEEVDPTEALPVLPGNAHTLWTPDSTGGDQEDVSLEEVRELLATLEGLDLDGEDWLPRELEEEGPQEQPEEEVTPGHEEEEPVAPSTVAPRWLEEEAALQLALHRSLEPQGQVAEQEEAAALRQALTLSLLEQPPLEAEEPPDGGTDGKAQLVVHSAFEQDVEELDRALRAALEVHVQEETVGPWRRTLPAELRARLERCHGVSVALRGDCTILRGFGAHPARAARHLVALLAGPWDQSLAFPLAASGPTLAGQTLKGPWNNLERLAENTGEFQEVVRAFYDTLDAARSSIRVVRVERVSHPLLQQQYELYRERLLQRCERRPVEQVLYHGTTAPAVPDICAHGFNRSFCGRNATVYGKGVYFARRASLSVQDRYSPPNADGHKAVFVARVLTGDYGQGRRGLRAPPLRGPGHVLLRYDSAVDCICQPSIFVIFHDTQALPTHLITCEHVPRASPDDPSGLPGRSPDT AC9CCFCF9B83A989 poly(adpribose) Poly(ADPribose) Poly(ADPribose) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) amp Adenosine monophosphate 5'-Adenosine monophosphate 5'-Adenylic acid 5'-AMP 5'-O-phosphonoadenosine adenosine 5'-(dihydrogen phosphate) Adenosine 5'-monophosphate Adenosine 5'-phosphate ADENOSINE MONOPHOSPHATE adenosine phosphate Adenosine-5'-monophosphoric acid adenosine-5'P adenosini phosphas Adenylate Adenylic acid Ado5'P AMP fosfato de adenosina pA PAdo phosphate d'adenosine 5'-adenylic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) chd1l_human Chromodomain-helicase-DNA-binding protein 1-like CHD1L ALC1 Amplified in liver cancer protein 1 protein protein human Homo sapiens Human MERAGATSRGGQAPGFLLRLHTEGRAEAARVQEQDLRQWGLTGIHLRSYQLEGVNWLAQRFHCQNGCILGDEMGLGKTCQTIALFIYLAGRLNDEGPFLILCPLSVLSNWKEEMQRFAPGLSCVTYAGDKEERACLQQDLKQESRFHVLLTTYEICLKDASFLKSFPWSVLVVDEAHRLKNQSSLLHKTLSEFSVVFSLLLTGTPIQNSLQELYSLLSFVEPDLFSKEEVGDFIQRYQDIEKESESASELHKLLQPFLLRRVKAEVATELPKKTEVVIYHGMSALQKKYYKAILMKDLDAFENETAKKVKLQNILSQLRKCVDHPYLFDGVEPEPFEVGDHLTEASGKLHLLDKLLAFLYSGGHRVLLFSQMTQMLDILQDYMDYRGYSYERVDGSVRGEERHLAIKNFGQQPIFVFLLSTRAGGVGMNLTAADTVIFVDSDFNPQNDLQAAARAHRIGQNKSVKVIRLIGRDTVEEIVYRKAASKLQLTNMIIEGGHFTLGAQKPAADADLQLSEILKFGLDKLLASEGSTMDEIDLESILGETKDGQWVSDALPAAEGGSRDQEEGKNHMYLFEGKDYSKEPSKEDRKSFEQLVNLQKTLLEKASQEGRSLRNKGSVLIPGLVEGSTKRKRVLSPEELEDRQKKRQEAAAKRRRLIEEKKRQKEEAEHKKKMAWWESNNYQSFCLPSEESEPEDLENGEESSAELDYQDPDATSLKYVSGDVTHPQAGAEDALIVHCVDDSGHWGRGGLFTALEKRSAEPRKIYELAGKMKDLSLGGVLLFPVDDKESRNKGQDLLALIVAQHRDRSNVLSGIKMAALEEGLKKIFLAAKKKKASVHLPRIGHATKGFNWYGTERLIRKHLAARGIPTYIYYFPRSKSAVLHSQSSSSSSRQLVP 226A1F8A44A8F9FE adp-d-ribose ADP-D-ribose (Rib5)ppA 5-(adenosine 5'-pyrophosphoryl)-D-ribose A5'pp5Rib adenosine 5'-(trihydrogen diphosphate), P'-5-ester with D-ribose adenosine 5'-diphosphoribose adenosine 5'-pyrophosphate, 5'-5-ester with D-ribofuranose adenosine diphosphate ribose Adenosine diphosphate ribose AdoPPRib ADP ribose ADP-D-ribose ADP-Rib ADP-riboses ADPribose D-ribofuranose, 5-5'-ester with adenosine 5'-(trihydrogen pyrophosphate) ribose adenosinediphosphate adenosine 5'-[3-(D-ribofuranos-5-O-yl) dihydrogen diphosphate] small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) adp_d_ribose-rep-6 2 10 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 2 adp_d_ribose-rep-7 1 17 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0DTC1-PRO_0000449637:p.[Val228_Glu229delinsIleGln;Phe360Asn] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain VE IQ certain certain sequence position certain certain certain sequence position certain F N Thu Oct 29 23:04:09 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC1-PRO_0000449637:p.[Val228_Glu229delinsIleGln;Phe1178Asn]' ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1, Fig. 3a, Supp. 4B. adp_d_ribose-pro_0000449637-1 6 17 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3d adp_d_ribose-pro_0000449637-2 3 17 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1, Fig. 3c adp-pro_0000449637-1 6 11 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3d amp-pro_0000449637-1 6 15 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3d adp_d_ribose-pro_0000449637-3 5 17 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2b parp10-pro_0000449637-1 7 13 enzyme target enzyme target substrate substrate neutral component neutral component purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region adprib1res monoadenosine diphosphoribosyl (ADP-ribosyl) modified residue ADP-rybosylation (from NAD) ADPRib1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined catalytic domain sufficient to bind sufficient binding region sufficient to bind range range range range 10 enzyme enzyme neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) de-adp-ribosylation reaction de-ADP-ribosylation reaction Fig. 4a-b parp10-pro_0000449637-2 7 13 enzyme target enzyme target substrate substrate neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind range range range range region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region adprib1res monoadenosine diphosphoribosyl (ADP-ribosyl) modified residue ADP-rybosylation (from NAD) ADPRib1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 enzyme enzyme neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind range range range range de-adp-ribosylation reaction de-ADP-ribosylation reaction Fig. 4a-b Positive control biotin-parp10-1 8 12 enzyme target enzyme target substrate substrate neutral component neutral component purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 13 putative self putative self neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind range range range range adp ribosylation adp ribosylation reaction adp ribosylation Fig. 4a adp_d_ribose-pro_0000449637-4 4 14 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role bait bait purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4c chd1l-adp_d_ribose-1 4 16 unspecified role unspecified role bait bait purified purified macro domain sufficient to bind sufficient binding region sufficient to bind range range range range 14 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig. 4c adp_d_ribose-p0dtc1_pro_0000449637-1 2 17 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 2