IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Flavonoids with inhibitory activity against SARS-CoV-2 3CLpro. Flavonoids with inhibitory activity against SARS-CoV-2 3CLpro. Journal of enzyme inhibition and medicinal chemistry 2020 imex curation Jo S., Kim S., Kim DY., Kim MS., Shin DH. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dhshin55@ewha.ac.kr 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Jo S., Kim S., Kim DY., Kim MS., Shin DH. Virus - Publications including interactions involving viral proteins FRET based assay, with substrate Dabcyl-KTSAVLQSGFRKME-Edans peptide. A complementary fluorophore (EDANS) and quencher (DABCYL) were attached to either end of a cleavable enzyme substrate (KTSAVLQSGFRKME). Upon enzymatic cleavage of the substrate, the fluorophore and quencher are separated and emission is restored, producing a signal that is directly correlated to the activity of the enzyme. dhshin55@ewha.ac.kr Accepted 2020-AUG-20 AT 08:57 BST AT 08:57 BST by ORCHARD Journal of enzyme inhibition and medicinal chemistry 2020 Only protein-protein interactions imex curation imex curation Flavonoids with inhibitory activity against SARS-CoV-2 3CLpro. Flavonoids with inhibitory activity against SARS-CoV-2 3CLpro. Journal of enzyme inhibition and medicinal chemistry 2020 imex curation Jo S., Kim S., Kim DY., Kim MS., Shin DH. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dhshin55@ewha.ac.kr 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Jo S., Kim S., Kim DY., Kim MS., Shin DH. Virus - Publications including interactions involving viral proteins dhshin55@ewha.ac.kr Accepted 2020-AUG-20 AT 08:57 BST AT 08:57 BST by ORCHARD Journal of enzyme inhibition and medicinal chemistry 2020 Only protein-protein interactions imex curation imex curation baicalin baicalin 5,6,7-trihydroxyflavone 7-O-beta-D-glucuronide 5,6-dihydroxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranosiduronic acid 7-D-glucuronic acid-5,6-dihydroxyflavone Baicalein 7-O-glucuronide Baicalin 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) pectolinarin Pectolinarin small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) p0dtd1-pro_0000449623 3C-like proteinase nsp5 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ 3264 3569 8-hydroxykaempferol 8-hydroxykaempferol small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) peptide substrate peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KTSAVLQSGFRKME peptide substrate-rep-3 1 7 enzyme target enzyme target substrate substrate neutral component neutral component EDANS fluorophore fluorophore c-term range C-terminal range c-term range c-term range C-terminal range c-term range Dabycl quencher dye labelled dye label dye labelled n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 5 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) protein cleavage protein cleavage Figure 2 peptide substrate-rep-4 1 3 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 7 enzyme target enzyme target substrate substrate neutral component neutral component EDANS fluorophore fluorophore c-term range C-terminal range c-term range c-term range C-terminal range c-term range Dabycl quencher dye labelled dye label dye labelled n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 5 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) protein cleavage protein cleavage Figure 2 8_hydroxykaempferol-peptide substrate-1 1 6 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 7 enzyme target enzyme target substrate substrate neutral component neutral component EDANS fluorophore fluorophore c-term range C-terminal range c-term range c-term range C-terminal range c-term range Dabycl quencher dye labelled dye label dye labelled n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 5 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) protein cleavage protein cleavage Figure 2 pectolinarin-peptide substrate-1 1 4 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 7 enzyme target enzyme target substrate substrate neutral component neutral component EDANS fluorophore fluorophore c-term range C-terminal range c-term range c-term range C-terminal range c-term range Dabycl quencher dye labelled dye label dye labelled n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 5 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) protein cleavage protein cleavage Figure 2 pectolinarin-peptide substrate-2 2 4 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 3 8_hydroxykaempferol-peptide substrate-2 2 6 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 3 baicalin-peptide substrate-1 2 3 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 3