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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>IntAct</shortLabel>
        <fullName>European Bioinformatics Institute</fullName>
        <alias type="synonym" typeAc="MI:1041">IntAct</alias>
        <alias type="synonym" typeAc="MI:1041">intact</alias>
      </names>
      <bibref>
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      </bibref>
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      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
        <attribute name="search-url" nameAc="MI:0615">http://www.ebi.ac.uk/intact/query/${ac}</attribute>
        <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
        <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact</attribute>
        <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Cryo-EM analysis of the post-fusion structure of the SARS-CoV spike glycoprotein.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="32681106" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-25762704" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-28210" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Cryo-EM analysis of the post-fusion structure of the SARS-CoV spike glycoprotein.</attribute>
            <attribute name="journal" nameAc="MI:0885">Nature communications</attribute>
            <attribute name="publication year" nameAc="MI:0886">2020</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Fan X., Cao D., Kong L., Zhang X.</attribute>
            <attribute name="dataset" nameAc="MI:0875">Virus - Publications including interactions involving viral proteins</attribute>
            <attribute name="dataset" nameAc="MI:0875">Coronavirus - Interactions investigated in the context of Coronavirus</attribute>
            <attribute name="contact-email" nameAc="MI:0634">caodf@ibp.ac.cn, xzzhang@ibp.ac.cn</attribute>
            <attribute name="author-announcement">28-Sep-2020: Contacted by IntAct-Help.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          </attributeList>
        </bibref>
        <xref>
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          <secondaryRef db="emdb" dbAc="MI:0936" id="EMD-30072" refType="identity" refTypeAc="MI:0356"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="7111">
            <names>
              <shortLabel>trini-high_5</shortLabel>
              <fullName>Trichoplusia ni cell line from eggs</fullName>
            </names>
            <cellType>
              <names>
                <shortLabel>high_5</shortLabel>
                <fullName>Insect cell line from Trichoplusia ni egg cells</fullName>
              </names>
              <xref>
                <primaryRef db="brenda" dbAc="MI:0864" id="BTO:0003619" refType="identity" refTypeAc="MI:0356"/>
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              </xref>
              <attributeList>
                <attribute name="comment" nameAc="MI:0612">Invitrogen supplies this cell line.</attribute>
                <attribute name="comment" nameAc="MI:0612">Invitrogen supply this cell line.</attribute>
              </attributeList>
            </cellType>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>3d-em-single</shortLabel>
            <fullName>electron microscopy 3D single particle reconstruction</fullName>
            <alias type="synonym" typeAc="MI:1041">3D-EM-single</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:2339" refType="identity" refTypeAc="MI:0356"/>
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        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>structure determination</shortLabel>
            <fullName>identification by structure determination</fullName>
            <alias type="synonym" typeAc="MI:1041">identification by structure determination</alias>
            <alias type="synonym" typeAc="MI:1041">structure determination</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:2287" refType="identity" refTypeAc="MI:0356"/>
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        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Fan X., Cao D., Kong L., Zhang X.</attribute>
          <attribute name="dataset" nameAc="MI:0875">Virus - Publications including interactions involving viral proteins</attribute>
          <attribute name="remark-internal">there is no mention in methods that the signal peptide has been removed, but PDB entry sequence does not show it - so I am assuming pdb is correct here (??) - unlike the expression system which they have not stated correctly.</attribute>
          <attribute name="contact-email" nameAc="MI:0634">caodf@ibp.ac.cn, xzzhang@ibp.ac.cn</attribute>
          <attribute name="accepted">Accepted 2020-JUL-28 AT 08:15 BST AT 08:15 BST by PPORRAS</attribute>
          <attribute name="journal" nameAc="MI:0885">Nature communications</attribute>
          <attribute name="publication year" nameAc="MI:0886">2020</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>spike_sars</shortLabel>
          <fullName>Spike glycoprotein</fullName>
          <alias type="gene name" typeAc="MI:0301">S</alias>
          <alias type="gene name synonym" typeAc="MI:0302">E2</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Peplomer protein</alias>
          <alias type="orf name" typeAc="MI:0306">2</alias>
        </names>
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          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P59594" version="48" refType="identity" refTypeAc="MI:0356"/>
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          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q7T696" refType="secondary-ac" refTypeAc="MI:0360"/>
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          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-29105N"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-9679509"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-9683686"/>
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          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_828851.1"/>
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        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
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            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="694009">
          <names>
            <shortLabel>sars-cov</shortLabel>
            <fullName>Human SARS coronavirus</fullName>
            <alias type="synonym" typeAc="MI:1041">SARS-CoV</alias>
            <alias type="synonym" typeAc="MI:1041">Severe acute respiratory syndrome coronavirus</alias>
          </names>
        </organism>
        <sequence>MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein P59594 original sequence version: 48</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="3" imexId="IM-28210-1">
        <names>
          <shortLabel>s-28</shortLabel>
        </names>
        <xref>
          <primaryRef db="wwpdb" dbAc="MI:0805" id="6m3w" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="emdb" dbAc="MI:0936" id="EMD-30072" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-25763200" refType="identity" refTypeAc="MI:0356"/>
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        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="4">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
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                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
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                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <featureList>
              <feature id="5">
                <names>
                  <shortLabel>region</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-25763203" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>sufficient to bind</shortLabel>
                    <fullName>sufficient binding region</fullName>
                    <alias type="synonym" typeAc="MI:1041">sufficient to bind</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0442" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608899" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="688"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="1178"/>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
            <stoichiometry value="3"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Supp. table 1</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>