IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Architecture and self-assembly of the SARS-CoV-2 nucleocapsid protein. Architecture and self-assembly of the SARS-CoV-2 nucleocapsid protein. Protein science : a publication of the Protein Society 2020 imex curation Ye Q., West AMV., Silletti S., Corbett KD. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus kcorbett@ucsd.edu Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ye Q, West AMV, Silletti S, Corbett KD Virus - Publications including interactions involving viral proteins kcorbett@ucsd.edu Accepted 2020-MAY-29 AT 08:29 BST AT 08:29 BST by PPORRAS 2020 Only protein-protein interactions imex curation imex curation Architecture and self-assembly of the SARS-CoV-2 nucleocapsid protein. Architecture and self-assembly of the SARS-CoV-2 nucleocapsid protein. Protein science : a publication of the Protein Society 2020 imex curation Ye Q., West AMV., Silletti S., Corbett KD. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus kcorbett@ucsd.edu Only protein-protein interactions imex curation in vitro In vitro h1-h2 ms mass spectrometry study of hydrogen/deuterium exchange h1-h2 ms predetermined predetermined participant predetermined Ye Q, West AMV, Silletti S, Corbett KD Virus - Publications including interactions involving viral proteins kcorbett@ucsd.edu Accepted 2020-MAY-29 AT 08:29 BST AT 08:29 BST by PPORRAS 2020 Only protein-protein interactions imex curation imex curation Architecture and self-assembly of the SARS-CoV-2 nucleocapsid protein. Architecture and self-assembly of the SARS-CoV-2 nucleocapsid protein. Protein science : a publication of the Protein Society 2020 imex curation Ye Q., West AMV., Silletti S., Corbett KD. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus kcorbett@ucsd.edu Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ye Q, West AMV, Silletti S, Corbett KD Virus - Publications including interactions involving viral proteins kcorbett@ucsd.edu Accepted 2020-MAY-29 AT 08:29 BST AT 08:29 BST by PPORRAS 2020 Only protein-protein interactions imex curation imex curation ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA n-37 3 4 unspecified role unspecified role neutral component neutral component purified purified 2b_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table S1 1.42A Rwork=0.157 (0.253) RFree=0.173 (0.243) n-36 3 4 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 2b_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Table S1 1.45A Rwork=0.167 (0.288) RFree=0.180 (0.310) n-38 1 4 unspecified role unspecified role neutral component neutral component purified purified 4 unspecified role unspecified role neutral component neutral component partially purified partially purified physical association physical association Figure 3A N (FL) formed a large oligomer that eluted in the void volume. (M.Wt >670 kDa). n-39 1 4 unspecified role unspecified role neutral component neutral component partially purified partially purified 2b_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Figure 3D n-40 1 4 unspecified role unspecified role neutral component neutral component partially purified partially purified 2b3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Figure 3E n-41 2 4 unspecified role unspecified role neutral component neutral component purified purified 2b_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain possible binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component purified purified 2b_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain possible binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 4 I have NOT added the longer construct as "residues 360-394 were not detected in our HDX-MS analysis" - and they do not know what is binding anyway at C terminus of N2b3.