IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Biochemical characterization of SARS-CoV-2 nucleocapsid protein. Biochemical characterization of SARS-CoV-2 nucleocapsid protein. Biochemical and biophysical research communications 2020 imex curation Zeng W., Liu G., Ma H., Zhao D., Yang Y., Liu M., Mohammed A., Zhao C., Yang Y., Xie J., Ding C., Ma X., Weng J., Gao Y., He H., Jin T. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus hhl725@ustc.edu.cn,jint@ustc.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro crosslink cross-linking study crosslink weight by staining molecular weight estimation by staining weight by staining Zeng W., Liu G., Ma H., Zhao D., Yang Y., Liu M., Mohammed A., Zhao C., Yang Y., Xie J., Ding C., Ma X., Weng J., Gao Y., He H., Jin T. Virus - Publications including interactions involving viral proteins the Method reference for Cross-linking (no 31) does not mention this term;thus entered "wt by staining" for participant detection. Crosslinker;DSS -Disuccinimidyl suberate hhl725@ustc.edu.cn,jint@ustc.edu.cn Accepted 2021-MAR-15 AT 09:09 GMT AT 09:09 GMT by KALPANAP Biochemical and biophysical research communications 2020 Only protein-protein interactions imex curation imex curation Biochemical characterization of SARS-CoV-2 nucleocapsid protein. Biochemical characterization of SARS-CoV-2 nucleocapsid protein. Biochemical and biophysical research communications 2020 imex curation Zeng W., Liu G., Ma H., Zhao D., Yang Y., Liu M., Mohammed A., Zhao C., Yang Y., Xie J., Ding C., Ma X., Weng J., Gao Y., He H., Jin T. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus hhl725@ustc.edu.cn,jint@ustc.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) saxs x ray scattering saxs predetermined predetermined participant predetermined Zeng W., Liu G., Ma H., Zhao D., Yang Y., Liu M., Mohammed A., Zhao C., Yang Y., Xie J., Ding C., Ma X., Weng J., Gao Y., He H., Jin T. Virus - Publications including interactions involving viral proteins hhl725@ustc.edu.cn,jint@ustc.edu.cn Accepted 2021-MAR-15 AT 09:09 GMT AT 09:09 GMT by KALPANAP Biochemical and biophysical research communications 2020 Only protein-protein interactions imex curation imex curation Biochemical characterization of SARS-CoV-2 nucleocapsid protein. Biochemical characterization of SARS-CoV-2 nucleocapsid protein. Biochemical and biophysical research communications 2020 imex curation Zeng W., Liu G., Ma H., Zhao D., Yang Y., Liu M., Mohammed A., Zhao C., Yang Y., Xie J., Ding C., Ma X., Weng J., Gao Y., He H., Jin T. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus hhl725@ustc.edu.cn,jint@ustc.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) sls static light scattering sls predetermined predetermined participant predetermined Zeng W., Liu G., Ma H., Zhao D., Yang Y., Liu M., Mohammed A., Zhao C., Yang Y., Xie J., Ding C., Ma X., Weng J., Gao Y., He H., Jin T. Virus - Publications including interactions involving viral proteins hhl725@ustc.edu.cn,jint@ustc.edu.cn Accepted 2021-MAR-15 AT 09:09 GMT AT 09:09 GMT by KALPANAP Biochemical and biophysical research communications 2020 Only protein-protein interactions imex curation imex curation Biochemical characterization of SARS-CoV-2 nucleocapsid protein. Biochemical characterization of SARS-CoV-2 nucleocapsid protein. Biochemical and biophysical research communications 2020 imex curation Zeng W., Liu G., Ma H., Zhao D., Yang Y., Liu M., Mohammed A., Zhao C., Yang Y., Xie J., Ding C., Ma X., Weng J., Gao Y., He H., Jin T. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus hhl725@ustc.edu.cn,jint@ustc.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Zeng W., Liu G., Ma H., Zhao D., Yang Y., Liu M., Mohammed A., Zhao C., Yang Y., Xie J., Ding C., Ma X., Weng J., Gao Y., He H., Jin T. Virus - Publications including interactions involving viral proteins hhl725@ustc.edu.cn,jint@ustc.edu.cn Accepted 2021-MAR-15 AT 09:09 GMT AT 09:09 GMT by KALPANAP Biochemical and biophysical research communications 2020 Only protein-protein interactions imex curation imex curation dsdna probe ds dna double stranded deoxyribonucleic acid ds DNA ds dna chemical synthesis Chemical synthesis (Chemical synthesis) TCGTCGTTTTGTCG ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA 123qwe-1 2 6 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range direct interaction direct interaction Table S1,Figure 2C The molecular mass (MM) of the solute estimated from the forward scattering I(0) and also from the Porod volume are both close to that of a dimer (~100 kDa). n-82 3 6 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range direct interaction direct interaction Figure 2A n-83 1 6 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 2B n-84 1 6 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 2B A small portion of higher-order oligomers was also observed by cross-linking. n-85 4 5 unspecified role unspecified role neutral component neutral component 5-prime fluorescein fluorescein label fluorescein certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range direct interaction direct interaction Figure 3C