IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Severe acute respiratory syndrome coronavirus ORF3a protein activates the NLRP3 inflammasome by promoting TRAF3-dependent ubiquitination of ASC. Severe acute respiratory syndrome coronavirus ORF3a protein activates the NLRP3 inflammasome by promoting TRAF3-dependent ubiquitination of ASC. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 imex curation Siu KL., Yuen KS., Castaño-Rodriguez C., Ye ZW., Yeung ML., Fung SY., Yuan S., Chan CP., Yuen KY., Enjuanes L., Jin DY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dyjin@hku.hk 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Siu KL., Yuen KS., Castaño-Rodriguez C., Ye ZW., Yeung ML., Fung SY., Yuan S., Chan CP., Yuen KY., Enjuanes L., Jin DY. Virus - Publications including interactions involving viral proteins Flag-TRAF3 and ASC were found to be of mouse origin from 25847972 (back reference 52) dyjin@hku.hk Accepted 2020-AUG-05 AT 13:10 BST AT 13:10 BST by LIVIAP FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Only protein-protein interactions imex curation imex curation Severe acute respiratory syndrome coronavirus ORF3a protein activates the NLRP3 inflammasome by promoting TRAF3-dependent ubiquitination of ASC. Severe acute respiratory syndrome coronavirus ORF3a protein activates the NLRP3 inflammasome by promoting TRAF3-dependent ubiquitination of ASC. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 imex curation Siu KL., Yuen KS., Castaño-Rodriguez C., Ye ZW., Yeung ML., Fung SY., Yuan S., Chan CP., Yuen KY., Enjuanes L., Jin DY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dyjin@hku.hk 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-thp-1 Homo sapiens acute monocytic leukemia cells thp-1 Human acute monocytic leukemia cell line anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Siu KL., Yuen KS., Castaño-Rodriguez C., Ye ZW., Yeung ML., Fung SY., Yuan S., Chan CP., Yuen KY., Enjuanes L., Jin DY. Virus - Publications including interactions involving viral proteins dyjin@hku.hk Accepted 2020-AUG-05 AT 13:10 BST AT 13:10 BST by LIVIAP FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Only protein-protein interactions imex curation imex curation Severe acute respiratory syndrome coronavirus ORF3a protein activates the NLRP3 inflammasome by promoting TRAF3-dependent ubiquitination of ASC. Severe acute respiratory syndrome coronavirus ORF3a protein activates the NLRP3 inflammasome by promoting TRAF3-dependent ubiquitination of ASC. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 imex curation Siu KL., Yuen KS., Castaño-Rodriguez C., Ye ZW., Yeung ML., Fung SY., Yuan S., Chan CP., Yuen KY., Enjuanes L., Jin DY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dyjin@hku.hk 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Siu KL., Yuen KS., Castaño-Rodriguez C., Ye ZW., Yeung ML., Fung SY., Yuan S., Chan CP., Yuen KY., Enjuanes L., Jin DY. Virus - Publications including interactions involving viral proteins Origin of Flag-TRAF2 inferred to be human by 10518213 (back reference 55); Flag-TRAF3 and ASC were found to be of mouse origin from 25847972 (back reference 52);Origin of Flag-TRAF6 was not traceable dyjin@hku.hk Accepted 2020-AUG-05 AT 13:10 BST AT 13:10 BST by LIVIAP FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Only protein-protein interactions imex curation imex curation asc_mouse Apoptosis-associated speck-like protein containing a CARD Pycard Asc PYD and CARD domain-containing protein protein protein mouse Mus musculus Mouse MGRARDAILDALENLSGDELKKFKMKLLTVQLREGYGRIPRGALLQMDAIDLTDKLVSYYLESYGLELTMTVLRDMGLQELAEQLQTTKEESGAVAAAASVPAQSTARTGHFVDQHRQALIARVTEVDGVLDALHGSVLTEGQYQAVRAETTSQDKMRKLFSFVPSWNLTCKDSLLQALKEIHPYLVMDLEQS DIP protein Q9EPB4 original sequence version: 106 DIP protein Q9EPB4 original sequence version: 104 DIP protein Q9EPB4 original sequence version: 103 2A4EA40194870B31 traf3_human TNF receptor-associated factor 3 TRAF3 CAP1 CRAF1 CD40 receptor-associated factor 1 CD40-binding protein LMP1-associated protein 1 CAP-1 RING-type E3 ubiquitin transferase TRAF3 protein protein human Homo sapiens Human MESSKKMDSPGALQTNPPLKLHTDRSAGTPVFVPEQGGYKEKFVKTVEDKYKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKDKVFKDNCCKREILALQIYCRNESRGCAEQLMLGHLLVHLKNDCHFEELPCVRPDCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIALQKHEDTDCPCVVVSCPHKCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIKDDTIFIKVIVDTSDLPDP DIP protein Q13114 original sequence version: 158 DIP protein Q13114 original sequence version: 127 DIP protein Q13114 original sequence version: 146 DIP protein Q13114 original sequence version: 151 9456E440C0A90FBF traf3_mouse TNF receptor-associated factor 3 CD40 receptor-associated factor 1 TRAFAMN Traf3 Craf1 Trafamn RING-type E3 ubiquitin transferase TRAF3 protein protein mouse Mus musculus Mouse MESSKKMDAAGTLQPNPPLKLQPDRGAGSVLVPEQGGYKEKFVKTVEDKYKCEKCRLVLCNPKQTECGHRFCESCMAALLSSSSPKCTACQESIIKDKVFKDNCCKREILALQVYCRNEGRGCAEQLTLGHLLVHLKNECQFEELPCLRADCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIKLQKHEDTDCPCVVVSCPHKCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQAARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIKDDTIFIKVIVDTSDLPDP DIP protein Q60803 original sequence version: 127 DIP protein Q60803 original sequence version: 129 DIP protein Q60803 original sequence version: 117 DIP protein Q60803 original sequence version: 121 EC282EFFF84E5A3F asc_human Apoptosis-associated speck-like protein containing a CARD PYD and CARD domain-containing protein Target of methylation-induced silencing 1 Caspase recruitment domain-containing protein 5 ASC CARD5 TMS1 PYCARD protein protein human Homo sapiens Human MGRARDAILDALENLTAEELKKFKLKLLSVPLREGYGRIPRGALLSMDALDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS DIP protein Q9ULZ3 original sequence version: 116 455987286586F46A traf2_human TNF receptor-associated factor 2 TRAF2 TRAP3 Tumor necrosis factor type 2 receptor-associated protein 3 E3 ubiquitin-protein ligase TRAF2 RING-type E3 ubiquitin transferase TRAF2 protein protein human Homo sapiens Human MAAASVTPPGSLELLQPGFSKTLLGTKLEAKYLCSACRNVLRRPFQAQCGHRYCSFCLASILSSGPQNCAACVHEGIYEEGISILESSSAFPDNAARREVESLPAVCPSDGCTWKGTLKEYESCHEGRCPLMLTECPACKGLVRLGEKERHLEHECPERSLSCRHCRAPCCGADVKAHHEVCPKFPLTCDGCGKKKIPREKFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTAEACSRQHRLDQDKIEALSSKVQQLERSIGLKDLAMADLEQKVLEMEASTYDGVFIWKISDFARKRQEAVAGRIPAIFSPAFYTSRYGYKMCLRIYLNGDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLMLLDQNNREHVIDAFRPDVTSSSFQRPVNDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAIVDLTGL DIP protein Q12933 original sequence version: 131 DIP protein Q12933 original sequence version: 129 DIP protein Q12933 original sequence version: 167 DIP protein Q12933 original sequence version: 193 DIP protein Q12933-1 original sequence version: 154 DIP protein Q12933 original sequence version: 130 DIP protein Q12933 original sequence version: 151 C508BE185B783B20 j9tc74_sars sars3a ORF3a protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MDLFMRFFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSATKIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAGMEAQFLYLYALIYFLQCINACRIIMRCWLCWKCKSKNPLLYDANYFVCWHTHNYDYCIPYNSVTDTIVVTEGDGISTPKLKEDYQIGGYSEDRHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDPPNVQIHTIDGSSGVANPAMDPIYDEPTTTTSVPL sars3a-traf2-1 3 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed J9TC74:p.[Pro36Ala;Gln38Ala;Ser40Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain Q A certain certain sequence position certain certain certain sequence position certain S A Fri Sep 18 22:11:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro36Ala;Gln38Ala;Ser40Ala]' tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5a panel 1-2 traf3-sars3a-1 3 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed J9TC74:p.[Pro36Ala;Gln38Ala;Ser40Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain Q A certain certain sequence position certain certain certain sequence position certain S A Wed Sep 16 10:21:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro36Ala;Gln38Ala;Ser40Ala]' tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5a panel 3-4 pycard-sars3a-1 1 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed J9TC74:p.[Pro36Ala;Gln38Ala;Ser40Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain Q A certain certain sequence position certain certain certain sequence position certain S A Thu Sep 17 11:38:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro36Ala;Gln38Ala;Ser40Ala]' tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5a panel 7-8 pycard-1 2 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 5c