IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells. Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells. The Journal of biological chemistry 2018 imex curation Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus kyyuen@hku.hk,jfwchan@hku.hk 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus The range of S1 in spike glycoprotein and its tag features were annotated from back referenced 27489282 The viral strain was provided by Ron Fouchier, which is Human betacoronavirus 2c EMC/2012 isolate, with GenBank accession number, JX869059 (from PMID 23075143); S glycoprotein accession was chosen based on AFS88936.1 id provided in the GenBank submission kyyuen@hku.hk,jfwchan@hku.hk Accepted 2020-MAR-26 AT 08:48 GMT AT 08:48 GMT by MARTA The Journal of biological chemistry 2018 Only protein-protein interactions imex curation imex curation Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells. Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells. The Journal of biological chemistry 2018 imex curation Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus kyyuen@hku.hk,jfwchan@hku.hk 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus The range of S1 in spike glycoprotein and its tag features were annotated from back referenced 27489282 The viral strain was provided by Ron Fouchier, which is Human betacoronavirus 2c EMC/2012 isolate, with GenBank accession number, JX869059 (from PMID 23075143); S glycoprotein accession was chosen based on AFS88936.1 id provided in the GenBank submission kyyuen@hku.hk,jfwchan@hku.hk Accepted 2020-MAR-26 AT 08:48 GMT AT 08:48 GMT by MARTA The Journal of biological chemistry 2018 Only protein-protein interactions imex curation imex curation Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells. Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells. The Journal of biological chemistry 2018 imex curation Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus kyyuen@hku.hk,jfwchan@hku.hk 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-beas_2b Human bronchial epithelial cell line BEAS-2B beas_2b Human bronchial epithelial cell line BEAS-2B http://cancerres.aacrjournals.org/cgi/content/abstract/48/7/1904?ijkey=330aa4101c1c77d6fbc2b1fc3d60b4ead4af2511&keytype2=tf_ipsecsha anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Sorry Marta, i curated for wrong PMID last time... The range of S1 in spike glycoprotein and its tag features were annotated from back referenced 27489282 The viral strain was provided by Ron Fouchier, which is Human betacoronavirus 2c EMC/2012 isolate, with GenBank accession number, JX869059 (from PMID 23075143); S glycoprotein accession was chosen based on AFS88936.1 id provided in the GenBank submission kyyuen@hku.hk,jfwchan@hku.hk Accepted 2020-MAR-26 AT 08:48 GMT AT 08:48 GMT by MARTA The Journal of biological chemistry 2018 Only protein-protein interactions imex curation imex curation Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells. Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells. The Journal of biological chemistry 2018 imex curation Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus kyyuen@hku.hk,jfwchan@hku.hk 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus The range of S1 in spike glycoprotein and its tag features were annotated from back referenced 27489282 The viral strain was provided by Ron Fouchier, which is Human betacoronavirus 2c EMC/2012 isolate, with GenBank accession number, JX869059 (from PMID 23075143); S glycoprotein accession was chosen based on AFS88936.1 id provided in the GenBank submission kyyuen@hku.hk,jfwchan@hku.hk Accepted 2020-MAR-26 AT 08:48 GMT AT 08:48 GMT by MARTA The Journal of biological chemistry 2018 Only protein-protein interactions imex curation imex curation Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells. Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells. The Journal of biological chemistry 2018 imex curation Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus kyyuen@hku.hk,jfwchan@hku.hk 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-beas_2b Human bronchial epithelial cell line BEAS-2B beas_2b Human bronchial epithelial cell line BEAS-2B http://cancerres.aacrjournals.org/cgi/content/abstract/48/7/1904?ijkey=330aa4101c1c77d6fbc2b1fc3d60b4ead4af2511&keytype2=tf_ipsecsha anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus The range of S1 in spike glycoprotein and its tag features were annotated from back referenced 27489282 The viral strain was provided by Ron Fouchier, which is Human betacoronavirus 2c EMC/2012 isolate, with GenBank accession number, JX869059 (from PMID 23075143); S glycoprotein accession was chosen based on AFS88936.1 id provided in the GenBank submission kyyuen@hku.hk,jfwchan@hku.hk Accepted 2020-MAR-26 AT 08:48 GMT AT 08:48 GMT by MARTA The Journal of biological chemistry 2018 Only protein-protein interactions imex curation imex curation Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells. Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells. The Journal of biological chemistry 2018 imex curation Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus kyyuen@hku.hk,jfwchan@hku.hk 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation mesau-bhk21 Mesocricetus auratus_BHK21 bhk-21 Cells from kidneys of five unsexed, one-day-old hamsters BHK-21/C13 cell anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus bCoV-HKU9 spike glycoprotein Uniprot accession was taken from back referenced 27696819 The range of S1 in spike glycoprotein and its tag features were annotated from back referenced 27489282 The viral strain was provided by Ron Fouchier, which is Human betacoronavirus 2c EMC/2012 isolate, with GenBank accession number, JX869059 (from PMID 23075143); S glycoprotein accession was chosen based on AFS88936.1 id provided in the GenBank submission kyyuen@hku.hk,jfwchan@hku.hk Accepted 2020-MAR-26 AT 08:48 GMT AT 08:48 GMT by MARTA The Journal of biological chemistry 2018 Only protein-protein interactions imex curation imex curation k0brg7_mers Spike glycoprotein E2 Peplomer protein S protein protein cv2ce Human betacoronavirus 2c EMC/2012 MIHSVFLLMFLLTPTESYVDVGPDSVKSACIEVDIQQTFFDKTWPRPIDVSKADGIIYPQGRTYSNITITYQGLFPYQGDHGDMYVYSAGHATGTTPQKLFVANYSQDVKQFANGFVVRIGAAANSTGTVIISPSTSATIRKIYPAFMLGSSVGNFSDGKMGRFFNHTLVLLPDGCGTLLRAFYCILEPRSGNHCPAGNSYTSFATYHTPATDCSDGNYNRNASLNSFKEYFNLRNCTFMYTYNITEDEILEWFGITQTAQGVHLFSSRYVDLYGGNMFQFATLPVYDTIKYYSIIPHSIRSIQSDRKAWAAFYVYKLQPLTFLLDFSVDGYIRRAIDCGFNDLSQLHCSYESFDVESGVYSVSSFEAKPSGSVVEQAEGVECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSVNDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLILATVPHNLTTITKPLKYSYINKCSRLLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDYYRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKLEFANDTKIASQLGNCVEYSLYGVSGRGVFQNCTAVGVRQQRFVYDAYQNLVGYYSDDGNYYCLRACVSVPVSVIYDKETKTHATLFGSVACEHISSTMSQYSRSTRSMLKRRDSTYGPLQTPVGCVLGLVNSSLFVEDCKLPLGQSLCALPDTPSTLTPRSVRSVPGEMRLASIAFNHPIQVDQLNSSYFKLSIPTNFSFGVTQEYIQTTIQKVTVDCKQYVCNGFQKCEQLLREYGQFCSKINQALHGANLRQDDSVRNLFASVKSSQSSPIIPGFGGDFNLTLLEPVSISTGSRSARSAIEDLLFDKVTIADPGYMQGYDDCMQQGPASARDLICAQYVAGYKVLPPLMDVNMEAAYTSSLLGSIAGVGWTAGLSSFAAIPFAQSIFYRLNGVGITQQVLSENQKLIANKFNQALGAMQTGFTTTNEAFQKVQDAVNNNAQALSKLASELSNTFGAISASIGDIIQRLDVLEQDAQIDRLINGRLTTLNAFVAQQLVRSESAALSAQLAKDKVNECVKAQSKRSGFCGQGTHIVSFVVNAPNGLYFMHVGYYPSNHIEVVSAYGLCDAANPTNCIAPVNGYFIKTNNTRIVDEWSYTGSSFYAPEPITSLNTKYVAPQVTYQNISTNLPPPLLGNSTGIDFQDELDEFFKNVSTSIPNFGSLTQINTTLLDLTYEMLSLQQVVKALNESYIDLKELGNYTYYNKWPWYIWLGFIAGLVALALCVFFILCCTGCGTNCMGKLKCNRCCDRYEEYDLEPHKVHVH DIP protein K0BRG7 original sequence version: 3 DIP protein K0BRG7 original sequence version: 4 a3exg6-pro_0000291369 Spike protein S1 S E2 Peplomer protein 2 protein protein BtCoV-2 Bat coronavirus HKU9 BtCoV/HKU9 AAASRPECFNPRFTLTPLNHTLNYTSIKAKVSNVLLPDPYIAYSGQTLRQNLFMADMSNTILYPVTPPANGANGGFIYNTSIIPVSAGLFVNTWMYRQPASSRAYCQEPFGVAFGDTFENDRIAILIMAPDNLGSWSAVAPRNQTNIYLLVCSNATLCINPGFNRWGPAGSFIAPDALVDHSNSCFVNNTFSVNISTSRISLAFLFKDGDLLIYHSGWLPTSNFEHGFSRGSHPMTYFMSLPVGGNLPRAQFFQSIVRSNAIDKGDGMCTNFDVNLHVAHLINRDLLVSYFNNGSVANAADCADSAAEELYCVTGSFDPPTGVYPLSRYRAQVAGFVRVTQRGSYCTPPYSVLQDPPQPVVWRRYMLYDCVFDFTVVVDSLPTHQLQCYGVSPRRLASMCYGSVTLDVMRINETHLNNLFNRVPDTFSLYNYALPDNFYGCLHAFYLNSTAPYAVANRFPIKPGGRQSNSAFIDTVINAAHYSPFSYVYGLAVITLKPAAGSKLVCPVANDTVVITDRCVQYNLYGYTGTGVLSKNTSLVIPDGKVFTASSTGTIIGVSINSTTYSIMPCVTVPVSVGYHPNFERALLFNGLSCSQRSRAVTEPVSVLWSASATAQDAFDTPSGCVVNVELRNTTIVNTCAMPIGNSLCFINGSIATANADSLPR 12 676 E395F622F07CEE08 bip_human Endoplasmic reticulum chaperone BiP HSPA5 GRP78 Immunoglobulin heavy chain-binding protein 78 kDa glucose-regulated protein Binding-immunoglobulin protein Heat shock protein 70 family protein 5 Heat shock protein family A member 5 protein protein human Homo sapiens Human MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL DIP protein P11021 original sequence version: 127 DIP protein P11021 original sequence version: 137 DIP protein P11021 original sequence version: 185 59B7D8D85BC32A00 hspa5-s-1 5 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range s1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 1c-e hspa5-s-2 6 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed electroporation electroporation s1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 2a s-hspa5-1 6 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed electroporation electroporation s1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 2b s-hspa5-2 4 9 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed s1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 2c s-hspa5-3 2 9 unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed s1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 2d hspa5-s-3 1 9 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role prey prey infection infection physical association physical association 2e hspa5-s-4 1 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role bait bait infection infection physical association physical association 2e hspa5-s-5 3 9 unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role prey prey infection infection physical association physical association 2e s-hspa5-4 3 7 unspecified role unspecified role bait bait infection infection 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 2e hspa5-s-6 6 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed electroporation electroporation c-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 8a