IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA. Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA. Proceedings of the National Academy of Sciences of the United States of America 2018 imex curation Ferron F., Subissi L., Silveira De Morais AT., Le NTT., Sevajol M., Gluais L., Decroly E., Vonrhein C., Bricogne G., Canard B., Imbert I. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus isabelle.imbert@univ-amu.fr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ferron F., Subissi L., Silveira De Morais AT., Le NTT., Sevajol M., Gluais L., Decroly E., Vonrhein C., Bricogne G., Canard B., Imbert I. Virus - Publications including interactions involving viral proteins isabelle.imbert@univ-amu.fr Accepted 2020-APR-27 AT 07:43 BST AT 07:43 BST by MGT Proceedings of the National Academy of Sciences of the United States of America 2018 Only protein-protein interactions imex curation imex curation Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA. Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA. Proceedings of the National Academy of Sciences of the United States of America 2018 imex curation Ferron F., Subissi L., Silveira De Morais AT., Le NTT., Sevajol M., Gluais L., Decroly E., Vonrhein C., Bricogne G., Canard B., Imbert I. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus isabelle.imbert@univ-amu.fr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ferron F., Subissi L., Silveira De Morais AT., Le NTT., Sevajol M., Gluais L., Decroly E., Vonrhein C., Bricogne G., Canard B., Imbert I. Virus - Publications including interactions involving viral proteins isabelle.imbert@univ-amu.fr Accepted 2020-APR-27 AT 07:43 BST AT 07:43 BST by MGT Proceedings of the National Academy of Sciences of the United States of America 2018 Only protein-protein interactions imex curation imex curation Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA. Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA. Proceedings of the National Academy of Sciences of the United States of America 2018 imex curation Ferron F., Subissi L., Silveira De Morais AT., Le NTT., Sevajol M., Gluais L., Decroly E., Vonrhein C., Bricogne G., Canard B., Imbert I. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus isabelle.imbert@univ-amu.fr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Ferron F., Subissi L., Silveira De Morais AT., Le NTT., Sevajol M., Gluais L., Decroly E., Vonrhein C., Bricogne G., Canard B., Imbert I. Virus - Publications including interactions involving viral proteins isabelle.imbert@univ-amu.fr Accepted 2020-APR-27 AT 07:43 BST AT 07:43 BST by MGT Proceedings of the National Academy of Sciences of the United States of America 2018 Only protein-protein interactions imex curation imex curation p0c6x7-pro_0000037318 RNA-directed RNA polymerase nsp12 rep ORF1ab polyprotein 1a-1b protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus SADASTFLNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNEKVAGFAKFLKTNCCRFQEKDEEGNLLDSYFVVKRHTMSNYQHEETIYNLVKDCPAVAVHDFFKFRVDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYANLGERVRQSLLKTVQFCDAMRDAGIVGVLTLDNQDLNGNWYDFGDFVQVAPGCGVPIVDSYYSLLMPILTLTRALAAESHMDADLAKPLIKWDLLKYDFTEERLCLFDRYFKYWDQTYHPNCINCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCFSVAALTNNVAFQTVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDIRQLLFVVEVVDKYFDCYDGGCINANQVIVNNLDKSAGFPFNKWGKARLYYDSMSYEDQDALFAYTKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIAATRGATVVIGTSKFYGGWHNMLKTVYSDVETPHLMGWDYPKCDRAMPNMLRIMASLVLARKHNTCCNLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNICQAVTANVNALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDHEFVDEFYAYLRKHFSMMILSDDAVVCYNSNYAAQGLVASIKNFKAVLYYQNNVFMSEAKCWTETDLTKGPHEFCSQHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYADVFHLYLQYIRKLHDELTGHMLDMYSVMLTNDNTSRYWEPEFYEAMYTPHTVLQ 4370 5301 80FB0EF691E4A10E p0c6x7-pro_0000037320 Proofreading exoribonuclease nsp14 rep ORF1ab polyprotein 1a-1b protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus AENVTGLFKDCSKIITGLHPTQAPTHLSVDIKFKTEGLCVDIPGIPKDMTYRRLISMMGFKMNYQVNGYPNMFITREEAIRHVRAWIGFDVEGCHATRDAVGTNLPLQLGFSTGVNLVAVPTGYVDTENNTEFTRVNAKPPPGDQFKHLIPLMYKGLPWNVVRIKIVQMLSDTLKGLSDRVVFVLWAHGFELTSMKYFVKIGPERTCCLCDKRATCFSTSSDTYACWNHSVGFDYVYNPFMIDVQQWGFTGNLQSNHDQHCQVHGNAHVASCDAIMTRCLAVHECFVKRVDWSVEYPIIGDELRVNSACRKVQHMVVKSALLADKFPVLHDIGNPKAIKCVPQAEVEWKFYDAQPCSDKAYKIEELFYSYATHHDKFTDGVCLFWNCNVDRYPANAIVCRFDTRVLSNLNLPGCDGGSLYVNKHAFHTPAFDKSAFTNLKQLPFFYYSDSPCESHGKQVVSDIDYVPLKSATCITRCNLGGAVCRHHANEYRQYLDAYNMMISAGFSLWIYKQFDTYNLWNTFTRLQ 5903 6429 D8E25737EF40967D p0c6x7-pro_0000037317 Non-structural protein 10 rep ORF1ab polyprotein 1a-1b protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus AGNATEVPANSTVLSFCAFAVDPAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLRNTVCTVCGMWKGYGCSCDQLREPLMQ 4231 4369 1F48DD3A6874826C q1t6x8_cvhsa-rep-1 2 5 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Table S1 Rwork: 0.193 Rfree: 0.235 3.38Å rep-rep-61 3 5 unspecified role unspecified role prey prey purified purified P0C6X7-PRO_0000037320:p.Tyr296Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 18:53:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr296Ala' P0C6X7-PRO_0000037320:p.Pro297Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain P A Tue Jun 30 18:52:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro297Ala' P0C6X7-PRO_0000037320:p.Cys473Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C A Tue Jun 30 15:37:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys473Ala' P0C6X7-PRO_0000037320:p.Thr475Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T A Tue Jun 30 15:43:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr475Ala' P0C6X7-PRO_0000037320:p.His487Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain H A Tue Jun 30 18:47:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His487Ala' P0C6X7-PRO_0000037320:p.Leu495Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 17:50:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu495Ala' P0C6X7-PRO_0000037320:p.Tyr498Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 19:36:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr498Ala' P0C6X7-PRO_0000037320:p.Asn499Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N A Tue Jun 30 16:04:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn499Ala' n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0C6X7-PRO_0000037320:p.Asn306Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N A Tue Jun 30 18:37:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn306Ala' P0C6X7-PRO_0000037320:p.[Asp90Ala;Glu92Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain E A Tue Jun 30 18:42:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp90Ala;Glu92Ala]' P0C6X7-PRO_0000037320:p.Arg310Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 16:38:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg310Ala' P0C6X7-PRO_0000037320:p.Val466Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain V K Tue Jun 30 19:42:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val466Lys' P0C6X7-PRO_0000037320:p.Leu468Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain L K Tue Jun 30 19:55:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu468Lys' P0C6X7-PRO_0000037320:p.Lys469Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 16:42:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys469Ala' P0C6X7-PRO_0000037320:p.Leu479Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 20:26:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu479Ala' ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. S6, Table S2 rep-rep-62 1 5 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 4 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig S4