HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Mitogen-activated Protein Kinase Kinase 2 (MEK2), a Novel E2-interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway. Mitogen-activated Protein Kinase Kinase 2 (MEK2), a Novel E2-interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway. Journal of virology 2016 imex curation Wang J., Chen S., Liao Y., Zhang E., Feng S., Yu S., Li LF., He WR., Li Y., Luo Y., Sun Y., Zhou M., Wang X., Munir M., Li S., Qiu HJ. Virus - Publications including interactions involving viral proteins lisu@hvri.ac.cn,huajiqiu@hvri.ac.cn. 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wang J., Chen S., Liao Y., Zhang E., Feng S., Yu S., Li LF., He WR., Li Y., Luo Y., Sun Y., Zhou M., Wang X., Munir M., Li S., Qiu HJ. Virus - Publications including interactions involving viral proteins lisu@hvri.ac.cn,huajiqiu@hvri.ac.cn. Su Li, lisu@hvri.ac.cn, or Hua-Ji Qiu, huajiqiu@hvri.ac.cn. Accepted 2018-JAN-19 AT 12:46 GMT AT 12:46 GMT by PPORRAS pig macrophage cDNA library Journal of virology 2016 Only protein-protein interactions imex curation imex curation Mitogen-activated Protein Kinase Kinase 2 (MEK2), a Novel E2-interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway. Mitogen-activated Protein Kinase Kinase 2 (MEK2), a Novel E2-interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway. Journal of virology 2016 imex curation Wang J., Chen S., Liao Y., Zhang E., Feng S., Yu S., Li LF., He WR., Li Y., Luo Y., Sun Y., Zhou M., Wang X., Munir M., Li S., Qiu HJ. Virus - Publications including interactions involving viral proteins lisu@hvri.ac.cn,huajiqiu@hvri.ac.cn. 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation pig-pk15 pig pk15 Pig pig-pk15 pig kidney pk15 confocal microscopy confocal microscopy immunostaining immunostaining Wang J., Chen S., Liao Y., Zhang E., Feng S., Yu S., Li LF., He WR., Li Y., Luo Y., Sun Y., Zhou M., Wang X., Munir M., Li S., Qiu HJ. Virus - Publications including interactions involving viral proteins lisu@hvri.ac.cn,huajiqiu@hvri.ac.cn. Su Li, lisu@hvri.ac.cn, or Hua-Ji Qiu, huajiqiu@hvri.ac.cn. Accepted 2018-JAN-19 AT 12:46 GMT AT 12:46 GMT by PPORRAS pig macrophage cDNA library Journal of virology 2016 Only protein-protein interactions imex curation imex curation Mitogen-activated Protein Kinase Kinase 2 (MEK2), a Novel E2-interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway. Mitogen-activated Protein Kinase Kinase 2 (MEK2), a Novel E2-interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway. Journal of virology 2016 imex curation Wang J., Chen S., Liao Y., Zhang E., Feng S., Yu S., Li LF., He WR., Li Y., Luo Y., Sun Y., Zhou M., Wang X., Munir M., Li S., Qiu HJ. Virus - Publications including interactions involving viral proteins lisu@hvri.ac.cn,huajiqiu@hvri.ac.cn. 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation pig-pk15 pig pk15 Pig pig-pk15 pig kidney pk15 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang J., Chen S., Liao Y., Zhang E., Feng S., Yu S., Li LF., He WR., Li Y., Luo Y., Sun Y., Zhou M., Wang X., Munir M., Li S., Qiu HJ. Virus - Publications including interactions involving viral proteins lisu@hvri.ac.cn,huajiqiu@hvri.ac.cn. Su Li, lisu@hvri.ac.cn, or Hua-Ji Qiu, huajiqiu@hvri.ac.cn. Accepted 2018-JAN-19 AT 12:46 GMT AT 12:46 GMT by PPORRAS Journal of virology 2016 Only protein-protein interactions imex curation imex curation Mitogen-activated Protein Kinase Kinase 2 (MEK2), a Novel E2-interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway. Mitogen-activated Protein Kinase Kinase 2 (MEK2), a Novel E2-interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway. Journal of virology 2016 imex curation Wang J., Chen S., Liao Y., Zhang E., Feng S., Yu S., Li LF., He WR., Li Y., Luo Y., Sun Y., Zhou M., Wang X., Munir M., Li S., Qiu HJ. Virus - Publications including interactions involving viral proteins lisu@hvri.ac.cn,huajiqiu@hvri.ac.cn. 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Wang J., Chen S., Liao Y., Zhang E., Feng S., Yu S., Li LF., He WR., Li Y., Luo Y., Sun Y., Zhou M., Wang X., Munir M., Li S., Qiu HJ. Virus - Publications including interactions involving viral proteins lisu@hvri.ac.cn,huajiqiu@hvri.ac.cn. Su Li, lisu@hvri.ac.cn, or Hua-Ji Qiu, huajiqiu@hvri.ac.cn. Accepted 2018-JAN-19 AT 12:46 GMT AT 12:46 GMT by PPORRAS Journal of virology 2016 Only protein-protein interactions imex curation imex curation Mitogen-activated Protein Kinase Kinase 2 (MEK2), a Novel E2-interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway. Mitogen-activated Protein Kinase Kinase 2 (MEK2), a Novel E2-interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway. Journal of virology 2016 imex curation Wang J., Chen S., Liao Y., Zhang E., Feng S., Yu S., Li LF., He WR., Li Y., Luo Y., Sun Y., Zhou M., Wang X., Munir M., Li S., Qiu HJ. Virus - Publications including interactions involving viral proteins lisu@hvri.ac.cn,huajiqiu@hvri.ac.cn. 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang J., Chen S., Liao Y., Zhang E., Feng S., Yu S., Li LF., He WR., Li Y., Luo Y., Sun Y., Zhou M., Wang X., Munir M., Li S., Qiu HJ. Virus - Publications including interactions involving viral proteins lisu@hvri.ac.cn,huajiqiu@hvri.ac.cn. Su Li, lisu@hvri.ac.cn, or Hua-Ji Qiu, huajiqiu@hvri.ac.cn. Accepted 2018-JAN-19 AT 12:46 GMT AT 12:46 GMT by PPORRAS pig macrophage cDNA library Journal of virology 2016 Only protein-protein interactions imex curation imex curation Mitogen-activated Protein Kinase Kinase 2 (MEK2), a Novel E2-interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway. Mitogen-activated Protein Kinase Kinase 2 (MEK2), a Novel E2-interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway. Journal of virology 2016 imex curation Wang J., Chen S., Liao Y., Zhang E., Feng S., Yu S., Li LF., He WR., Li Y., Luo Y., Sun Y., Zhou M., Wang X., Munir M., Li S., Qiu HJ. Virus - Publications including interactions involving viral proteins lisu@hvri.ac.cn,huajiqiu@hvri.ac.cn. 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunostaining immunostaining Wang J., Chen S., Liao Y., Zhang E., Feng S., Yu S., Li LF., He WR., Li Y., Luo Y., Sun Y., Zhou M., Wang X., Munir M., Li S., Qiu HJ. Virus - Publications including interactions involving viral proteins lisu@hvri.ac.cn,huajiqiu@hvri.ac.cn. Su Li, lisu@hvri.ac.cn, or Hua-Ji Qiu, huajiqiu@hvri.ac.cn. Accepted 2018-JAN-19 AT 12:46 GMT AT 12:46 GMT by PPORRAS pig macrophage cDNA library Journal of virology 2016 Only protein-protein interactions imex curation imex curation Mitogen-activated Protein Kinase Kinase 2 (MEK2), a Novel E2-interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway. Mitogen-activated Protein Kinase Kinase 2 (MEK2), a Novel E2-interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway. Journal of virology 2016 imex curation Wang J., Chen S., Liao Y., Zhang E., Feng S., Yu S., Li LF., He WR., Li Y., Luo Y., Sun Y., Zhou M., Wang X., Munir M., Li S., Qiu HJ. Virus - Publications including interactions involving viral proteins lisu@hvri.ac.cn,huajiqiu@hvri.ac.cn. 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Wang J., Chen S., Liao Y., Zhang E., Feng S., Yu S., Li LF., He WR., Li Y., Luo Y., Sun Y., Zhou M., Wang X., Munir M., Li S., Qiu HJ. Virus - Publications including interactions involving viral proteins lisu@hvri.ac.cn,huajiqiu@hvri.ac.cn. Su Li, lisu@hvri.ac.cn, or Hua-Ji Qiu, huajiqiu@hvri.ac.cn. Accepted 2018-JAN-19 AT 12:46 GMT AT 12:46 GMT by PPORRAS pig macrophage cDNA library Journal of virology 2016 Only protein-protein interactions imex curation imex curation p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 lt_sv40 Large T antigen protein protein sv40 sv40 SV40 MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLFCSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETKCDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQAVDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPKMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLNFELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKRTQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALLLMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDDDEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFTCFKKPPTPPPEPET 3E37D4BAFC5D59BD i3l5d4_pig Protein kinase domain-containing protein MAP2K2 protein protein pig Sus scrofa (Pig) Pig MARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAKRIPEEILGKVSIAVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVELSIGRYPIPPPDAKELEAIFGRPMVDGGDGEPHSISPRPRPPGRPISGHGMDSRPAMAIFELLDYIVNEPPPKLPNGVFTQDFQEFVNKCLIKNPAERADLKMLMNHTFIKRSEVEEVDFAGWLCKTLRLNQPSTPTRTAV E4E2AFCD93DAD5A3 q5u8x5_9flav Autoprotease p20 Capsid protein C Cysteine protease NS2 E(rns) glycoprotein Envelope glycoprotein E1 Envelope glycoprotein E2 Genome polyprotein N-terminal protease NS5B Non-structural protein 2 Non-structural protein 2-3 Non-structural protein 3 Non-structural protein 4A Non-structural protein 4B Non-structural protein 5A RNA-directed RNA polymerase Serine protease NS3 gp33 gp44/48 gp55 Viroporin p7 protein protein classical swine fever virus Classical swine fever virus MELNHFELLYKTNKQKPMGVEEPVYDVTGRPLFGDPSEVHPQSTLKLPHDRGRGNIKTTLNNLPRKGDCRSGNHLGPVSGIYVKPGPVFYQDYMGPVYHRAPLEFFNEAQFCEVTKRIGRVTGSDGKLYHIYVCIDGCILLKLAKRGEPRTLKWIRNFTDCPLWVTSCSDDGASGSKEKKPDRINKGKLKIAPKEHEKDSRTKPPDATIVVEGVKYQVKKKGKVKGKNTQDGLYHNKNKPPESRKKLEKALLAWAVITIMLYQPVEAENITQWNLSDNGTNGIQHAMYLRGVSRSLHGIWPEKICKGVPTYLATDTELKEIQGMMDASEGTNYTCCKLQRHEWNKHGWCNWYNIDPWIQLMNRTQANLAEGPPAKECAVTCRYDKDADINVVTQARNRPTTLTGCKKGKNFSFAGTIIEGPCNFNVSVEDILYGDHECGSLLQDTALYLVDGMTNTIENARQGAARVTSWLGRQLSTAGKRLEGRSKTWFGAYALSPYCNVTSKIGYIWYTNNCTPACLPKNTKIIGPGKFDTNAEDGKILHEMGGHLSEFLLLSLVVLSDFAPETASALYLILHYVIPQSYEEPEGCDTNQLNLTVELRTEDVIPSSVWNVGKYVCVRPDWWPYETKVALLFEEAGQVVKLALRALRDLTRVWNSASTTAFLICLIKVLRGQIVQGVIWLLLVTGAQGRLACKEDYRYALSSTNEIGLLGAGGLTTTWEEYSHDLQLNDGTVKAICVAGSFKVTALNVVSRRYLASLHKGALLTSVTFELLFDGTNPSTEEMGDDFGFGLCPFDTSPVVKGKYNTTLLNGSAFYLVCPIGWTGVIECTAVSPTTLRTEVVKTFRREKPFPHRMDCVTTTVENEDLFYCKLGGNWTCVKGEPVVYTGGQVKQCKWCGFDFNEPDGLPHYPIGKCILANETGYRIVDSTDCNRDGVVISAEGSHECLIGNTTVKVHASDERLGPMPCRPKEIVSSAGPVRKTSCTFNYAKTLKNKYYEPRDSYFQQYMLKGEYQYWFDLDVTDRHSDYFAEFVVLVVVALLGGRYVLWLIVTYIVLTEQLAAGLPLGQGEVVLIGNLITHTDIEVVVYFLLLYLVMRDEPIKKWILLLFHAMTNNPVKTITVALLMVSGVAKGGKIDGGWQRLPETSFDIQLALTVIVVAVMLLAKRDPTTVPLVITVATLRTAKMTNGLSTDIAIATVSTALLTWTYISDYYRYKTWLQYLISTVTGIFLIRVLKGIGELDLHTPTLPSYRPLFFILVYLISTAVVTRWNLDIAGLLLQCVPTLLMVFTMWADILTLILILPTYELTKLYYLKEVKIGAERGWLWKTNFKRVNDIYEVDQAGEGVYLFPSKQKTSSITGTMLPLIKAILISCISNKWQFIYLLYLIFEVSYYLHKKIIDEIAGGTNFISRLVAALIEANWAFDNEEVRGLKKFFLLSSRVKELIIKHKVRNEVMVHWFGDEEVYGMPKLVGLVKAATLSKNKHCILCTVCEDREWKGETCPKCGRFGPPMTCGMTLADFEEKHYKRIFFREDQSEGPVREEYAGYLQYRARGQLFLRNLPVLATKVKMLLVGNLGTEVGDLEHLGWVLRGPAVCKKVTEHEKCTTSIMDKLTAFFGVMPRGTTPRAPVRFPTSLLKIRRGLETGWAYTHQGGISSVDHVTCGKDLLVCDTMGRTRVVCQSNNKMTDESEYGVKTDSGCPEGARCYVFNPEAVNISGTKGAMVHLQKTGGEFTCVTASGTPAFFDLKNLKGWSGLPIFEASSGRVVGRVKVGKNEDSKPTKLMSGIQTVSKSTTDLTEMVKKITTMNRGEFRQITLATGAGKTTELPRSVIEEIGRHKRVLVLIPLRAAAESVYQYMRQKHPSIAFNLRIGEMKEGDMATGITYASYGYFCQMPQPKLRAAMVEYSFIFLDEYHCATPEQLAIMGKIHRFSENLRVVAMTATPAGTVTTTGQKHPIEEFIAPEVMKGEDLGSEYLDIAGLKIPVEEMKSNMLVFVPTRNMAVETAKKLKAKGYNSGYYYSGEDPSNLRVVTSQSPYVVVATNAIESGVTLPDLDVVVDTGLKCEKRIRLSSKMPFIVTGLKRMAVTIGEQAQRRGRVGRVKPGRYYRSQETPVGSKDYHYDLLQAQRYGIEDGINITKSFREMNYDWSLYEEDSLMITQLEILNNLLISEELPMAVKNIMARTDHPEPIQLAYNSYETQVPVLFPKIRNGEVTDSYDNYTFLNARKLGDDVPPYVYATEDEDLAVELLGLDWPDPGNQGTVEAGRALKQVVGLSTAENALLVALFGYVGYQALSKRHIPVVTDIYSIEDHRLEDTTHLQYAPNAIKTEGKETELKELAQGDVQRCVEAMTNYAREGIQFMKSQALKVKETPTYKETMDTVTDYVKKFMEALKDSKEDIMKYGLWGTHTALYKSISARLGSETAFATLVVKWLAFGGESIADHVKQAATDLVVYYIINRPQFPGDTETQQEGRKFVASLLVSALATYTYKSWNYNNLSKIVEPALATLPYAATALKLFAPTRLESVVILSTAIYKTYLSIRRGKSDGLLGTGVSAAMEIMSQNPVSVGIAVMLGVGAVAAHNAIEASEQKRTLLMKVFVKNFLDQAATDELVKESPEKIIMALFEAVQTVGNPLRLVYHLYGVFYKGWEAKELAQRTAGRNLFTLIMFEAVELLGVDSEGKIRQLSSNYILELLYKFRDSIKSSVREMAISWAPAPFSCDWTPTDDRIGLPQDNFLQVETKCPCGYKMKAVKNCAGELRLLEEEGSFLCRNKFGRGSRNYKVTKYYDDNLSEIKPVIKMEGHVELYYKGATIKLDFNNSKTILATDKWEVDHSTLVRVLKRHTGAGYNGAYLGEKPNHKHLIERDCATITKDKVCFLKMKRGCAFTYDLSLHNLTRLIELVHKNNLEDKEIPAVTVTTWLAYTFVNEDIGTIRPAFGEKVTPEMQEEITLQPAVVVDTTDVTVTVVGEAPTMTTGETPTAFTSSGSDPKGQQVLKLGVGEGQYPGTNPQRASLHEAIQGADERPSVLILGSDKATSNRVKTAKNVKVYRGRDPLEVRDMMRRGKILVIALSRVDNALLKFVDYKGTFLTRETLEALSLGRPKKKDITKAEAQWLLCLEDQMEELPDWFAAGEPIFLEANIKHDRYHLVGDIATIKEKAKQLGATDSTKISKEVGAKVYSMKLSNWVMQEENKQGNLTPLFEELLQQCPPGGQNKTAHMVSAYQLAQGNWMPTSCHVFMGTISARRTKTHPYEAYVKLRELVEEHKMKTLCPGSSLGKHNDWIIGKIKYQGNLRTKHMLNPGKVAEQLCREGHRHNVYNKTIGSVMTATGIRLEKLPVVRAQTDTTNFHQAIRDKIDKEENLQTPGLHKKLMEVFNALKRPELESSYDAVEWEELERGINRKGAAGFFERKNIGEILDSEKNKVEEIIDNLKKGRNIKYYETAIPKNEKRDVNDDWTAGDFVDEKKPRVIQYPEAKTRLAITKVMYKWVKQKPVVIPGYEGKTPLFQIFDKVKKEWDQFQNPVAVSFDTKAWDTQVTTKDLELIKDIQKYYFKKKWHKFIDTLTMHMTEVPVICADGEVYIRKGQRGSGQPDTSAGNSMLNVLTMVYAFCEATGVPYKSFDRVAKIHVCGDDGFLITERALGEKFASKGVQILYEAGKPQKITEGDKMKVAYQFDDIEFCSHTPIQVRWSDNTSSYMPGRNTTTILAKMATRLDSSGERGTIAYEKAVAFSFLLMYSWNPLIRRICLLVLSTELQVKPGKSTTYYYEGDPISAYKEVIGHNLFDLKRTSFEKLAKLNLSMSVLGAWTRHTSKRLLQDCVNMGVKEGNWLVNADRLVSSKTGNRYIPGEGHTLQGRHYEELVLARKQINNFQGTDRYNLGPIVNMVLRRLRVMMMTLIGRGV 221D3B27E69E8E44 e2-mek2-1 1 10 unspecified role unspecified role prey prey living cell living cell cdna library cdna library region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell engineered engineered e2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association results q5u8x5_9flav-map2k2-1 5 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed engineered engineered n terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed engineered engineered e2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 1B q5u8x5_9flav-map2k2-2 7 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate engineered engineered n terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 11 unspecified role unspecified role bait bait engineered engineered purified purified e2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 1C q5u8x5_9flav-map2k2-3 6 10 unspecified role unspecified role neutral component neutral component n terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role neutral component neutral component e2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 1D-E e2-mek2-2 3 10 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level e2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2A q5u8x5_9flav-map2k2-4 7 10 unspecified role unspecified role bait bait engineered engineered purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate engineered engineered e2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 2C map2k2-1 7 10 unspecified role unspecified role bait bait engineered engineered purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate engineered engineered c terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 2E q5u8x5_9flav-map2k2-5 4 10 unspecified role unspecified role prey prey living cell living cell cdna library cdna library region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell engineered engineered e2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1A map2k2-q5u8x5_9flav-1 2 10 unspecified role unspecified role neutral component neutral component n terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role neutral component neutral component e2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 1D-E lt_sv40-tp53-2 4 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 1A