HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Networks of Host Factors that Interact with NS1 Protein of Influenza A Virus. Networks of Host Factors that Interact with NS1 Protein of Influenza A Virus. Frontiers in Microbiology 2015 imex curation Thulasi Raman SN., Zhou Y. Virus - Publications including interactions involving viral proteins Interactions depicted in figure 2 and supplementary table S1 were not included because the authors provided human UniProt accessions instead of the relevant pig ones. yan.zhou@usask.ca 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down western blot western blot Immuno blot Immuno blot Thulasi Raman SN., Zhou Y. Virus - Publications including interactions involving viral proteins Interactions depicted in figure 2 and supplementary table S1 were not included because the authors provided human UniProt accessions instead of the relevant pig ones. yan.zhou@usask.ca Accepted 2019-APR-23 AT 13:13 BST AT 13:13 BST by PPORRAS Frontiers in Microbiology 2015 Only protein-protein interactions imex curation imex curation Networks of Host Factors that Interact with NS1 Protein of Influenza A Virus. Networks of Host Factors that Interact with NS1 Protein of Influenza A Virus. Frontiers in Microbiology 2015 imex curation Thulasi Raman SN., Zhou Y. Virus - Publications including interactions involving viral proteins Interactions depicted in figure 2 and supplementary table S1 were not included because the authors provided human UniProt accessions instead of the relevant pig ones. yan.zhou@usask.ca 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Thulasi Raman SN., Zhou Y. Virus - Publications including interactions involving viral proteins Interactions depicted in figure 2 and supplementary table S1 were not included because the authors provided human UniProt accessions instead of the relevant pig ones. yan.zhou@usask.ca Accepted 2019-APR-23 AT 13:13 BST AT 13:13 BST by PPORRAS Frontiers in Microbiology 2015 Only protein-protein interactions imex curation imex curation q64hs0_9infa Non-structural protein 1 NS1 NS protein protein A/swine/Saskatchewan/18789/02(H1N1) Influenza A virus MDSNTITSFQVDCYLWHIRKLLSMRDMCDAPFDDRLRRDQKALKGRGSTLGLDLRVATMEGKKIVEDILKSEMDENLKIAIASSPAPRYITDMSIEEISREWYMLMPRQKITGGLMVKMDQAIMDKRIILKANFSVLFDQLETLVSLRAFTDNGAIVAEISPIPSMPGHSTEDVKNAIGILIGGLEWNDNSIRASENIQRFAWGVRDENGGPPLPPKQKRYMARRVESEV B6B5310B3382380F k7gp99_pig ILF3 protein protein pig Sus scrofa (Pig) Pig MRPVRIFVNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKVNSDNAESENVDVPQEDETKEGTGEQKTEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVTEDKYEILQSVDDAAIVIKNTKEPPLSLTIHLTSPVVREEMEKVLAGETLSVNDPPDVLDRQKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWGPLRGWPLELLCEKSIGTANRPMGAGEALRRVLECLASGIVMPDGSGIYDPCEKEATDAIGHLDRQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKMPKKPKNENPVDYTVQIPPSTTYAITPMKRPMEEDGDEKSPSKKKKKIQKKEEKAEPPQAMNALMRLNQLKPGLQYKLVSQTGPVHAPIFTMSVEVDGNSFEASGPSKKTAKLHVAVKVLQDMGLPTGAEGRDSKGEDSAEEMDTKPALLMPPPMMEALSAHSSLFCDPTAENVKQQGPILTKHGKNPVMELNEKRRGLKYELISETGGSHDKRFVMEVEVDGQKFQGAGSNKKVAKAYAAFAALEKLFPDTPLALDANKKKRVPVPVRGGPKFAAKPHNPVYGMGGPMHNEVPPPPNLRGRGRGGNIRGRGRGRGFGGANHGGYMNAGAGYGSYYGANSATAGYSDFFTDCYGYHDFGSS 04251D3A6A430C33 i3lk71_pig HNRNPC protein protein pig Sus scrofa (Pig) Pig MASNVTNKTDPRSMNSRVFIGNLNTLVVKKSDVEAIFSKYGKIVGCSVHKGFAFVQYVNERNARAAVAGEDGRMIAGQVLDINLAAEPKVNRGKAGVKRSAAEMYGSVPEHPSPSPLLSSSFDLDYDFQRDYYDRMYSYPARVPPPPPIARAVVPSKRQRVSGNTSRRGKSGFNSKSGQRGSSSKSGKLKGDDLQAIKKELTQIKQKVDSLLESLEKIEKEQSKQGVEMKNDKSEEEQSSSSQKKDETNVKMESEGGADDSAEEGDLLDDDDNEDRGDDQLELIKDDEKEAEEGEDDRDSANGEDDS C6490943C8E08601 f1s8l9_pig HNRNPU protein protein pig Sus scrofa (Pig) Pig MSSSPVNVKKLKVSELKEELKKRRLSDKGLKAELMERLQAALDDEEAGGRPAMEPGNGSLDLGGDSAERSGAGLEQEAAAGGDEEEEEEEEEEEGIAALDGDQMELGEENGAAGAADSGPMEEEEAASEDENGDDQGFQEGEDELGEEEEGAGDENGHGEQQPQPPAAPQQQPQQQRGAAKEAAGKSSGPTSLFAVTVAPPGARQGQQQAGGDGKTEQKGGDKKRGVKRPREDHGRGYFEYIEENKYSRAKSPQPPVEEEDEHFDDTVVCLDTYNCDLHFKISRDRLSASSLTMESFAFLWAGGRASYGVSKGKVCFEMKVTEKIPVRHLYTKDIDIHEVRIGWSLTTSGMLLGEEEFSYGYSLKGIKTCNCETEDYGEKFDENDVITCFANFESDEVELSYAKNGQDLGIAFKISKEVLAERPLFPHVLCHNCAVEFNFGQKEKPYFPIPEEYTFIQNVPLEDRVRGPKGPEEKKDCEVVMMIGLPGAGKTTWVTKHAAENPGKYNILGTNTIMDKMMVAGFKKQMADTGKLNTLLQRAPQCLGKFIEIAARKKRNFILDQTNVSAAAQRRKMCLFAGFQRKAVVVCPKDEDYKQRTQKKAEVEGKDLPEHAVLKMKGNFTLPEVAECFDEITYVELQKEEAQKLLEQYKEESKKALPPEKKQNTGSKKSNKNKSGKNQFNRGGGHRGRGGFNMRGGNFRGGAPGNRGGYNRRGNMPQRGGGGGGSGGIGYPYPRGPVFPSRGGYSNRGNYNRGGMPNRGNYNQNFRGRGNNRGYKNQSQGYNQWQQGSVHVNVLCGRVKGPK DC78F9BE2DF523C5 m3tyc8_pig ATP-dependent RNA helicase DDX1 DDX1 DEAD box protein 1 protein protein pig Sus scrofa (Pig) Pig MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLMAAETGSGKTGAFSIPVIQIVYETLKDQQEGKKGKTTIKTGASVLNKWQMNPYDRGSAFAIGSDGLCCQSREVKEWHGCRATKGLTKGKHYYEVSCHDQGLCRVGWSSMQASLDLGTDKFGFGFGGTGKKSHNKQFDNYGEEFTMHDTIGCYLDIDKGHVKFSKNGKDLGLAFEIPPHMKNQALFPACVLKNAELKFNFGEEEFKFPPKDGFVALSKASDSFVVKSQHSGNAQVAQTKFLPNAPKALIVEPSRELAEQTLNNIKQFKKYIDNPKLRELLIIGGVAARDQLSVLDNGVDIVVGTPGRLDDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFINRIHNQIPQITSDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPDTVHHVVVPVNPKTDRLWERLGKNHIRTDEVHAKDNTRPGANSPEMWSEAIKILKGEYTVRAIKEHKMDQAIIFCRTKIDCDNLEQYFMQQGGGPDKKGHQFSCVCLHGDRKPHERKQNLERFKKGDVRFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVATEKEKVWYHVCSSRGKGCYNTRLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVDEFDGKVTYGQKRTLGGGNYKGHVDILAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF 7E3A249281DB2E8A i3lqs0_pig Heterogeneous nuclear ribonucleoprotein K HNRNPK protein protein pig Sus scrofa (Pig) Pig METEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVELRILLQSKNAGAVIGKGGKNIKALRTDYNASVSVPDSSGPERILSISADIETIGEILKKIIPTLEEGLQLPSPTATSQLPLESDAVECLNYQHYKGSDFDCELRLLIHQSLAGGIIGVKGAKIKELRENTQTTIKLFQECCPQSTDRVVLIGGKPDRVVECIKIILDLISESPIKGRAQPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGGFDRMPPGRGGRPMPPSRRDYDDMSPRRGPPPPPPGRGGRGGSRARNLPLPPPPPPRGGDLMAYDRRGRPGDRYDGMVGFSADETWDSAIDTWSPSEWQMAYEPQGGSGYDYSYAGGRGSYGDLGGPIITTQVTIPKDLAGSIIGKGGQRIKQIRHESGASIKIDEPLEGSEDRIITITGTQDQIQNAQYLLQNSVKQYADVEGF 264011C2D457BF92 ns1-hnrnpc-1 2 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 6A hnrnpk-ns1-2 1 3 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 6B hnrnpu-ns1-2 1 3 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 6C ilf3-ns1-2 1 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 6C ddx1-ns1-2 1 3 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 6B