HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Herpes Simplex Virus 1 (HSV-1) and HSV-2 Mediate Species-Specific Modulations of Programmed Necrosis through the Viral Ribonucleotide Reductase Large Subunit R1 Herpes Simplex Virus 1 (HSV-1) and HSV-2 Mediate Species-Specific Modulations of Programmed Necrosis through the Viral Ribonucleotide Reductase Large Subunit R1 journal of virology 2015 imex curation Xiaoliang Yu, Yun Li,a Qin Chen,a Chenhe Su,b Zili Zhang,a Chengkui Yang,a Zhilin Hu,a Jue Hou,a Jinying Zhou,a Ling Gong,a Xuejun Jiang,c Chunfu Zheng,b,d Sudan Hea, Xiaoliang Yu, Yun Li,a Qin Chen,a Chenhe Su,b Zili Zhang,a Chengkui Yang,a Zhilin Hu,a Jue Hou,a Jinying Zhou,a Ling Gong,a Xuejun Jiang,c Chunfu Zheng,b,d Sudan Hea Virus - Publications including interactions involving viral proteins zheng.alan@hotmail.com 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xiaoliang Yu, Yun Li,a Qin Chen,a Chenhe Su,b Zili Zhang,a Chengkui Yang,a Zhilin Hu,a Jue Hou,a Jinying Zhou,a Ling Gong,a Xuejun Jiang,c Chunfu Zheng,b,d Sudan Hea, Xiaoliang Yu, Yun Li,a Qin Chen,a Chenhe Su,b Zili Zhang,a Chengkui Yang,a Zhilin Hu,a Jue Hou,a Jinying Zhou,a Ling Gong,a Xuejun Jiang,c Chunfu Zheng,b,d Sudan Hea Virus - Publications including interactions involving viral proteins zheng.alan@hotmail.com Accepted 2016-AUG-23 AT 11:53 BST AT 11:53 BST by MGT journal of virology 2015 imex curation Herpes Simplex Virus 1 (HSV-1) and HSV-2 Mediate Species-Specific Modulations of Programmed Necrosis through the Viral Ribonucleotide Reductase Large Subunit R1 Herpes Simplex Virus 1 (HSV-1) and HSV-2 Mediate Species-Specific Modulations of Programmed Necrosis through the Viral Ribonucleotide Reductase Large Subunit R1 journal of virology 2015 imex curation Xiaoliang Yu, Yun Li,a Qin Chen,a Chenhe Su,b Zili Zhang,a Chengkui Yang,a Zhilin Hu,a Jue Hou,a Jinying Zhou,a Ling Gong,a Xuejun Jiang,c Chunfu Zheng,b,d Sudan Hea, Xiaoliang Yu, Yun Li,a Qin Chen,a Chenhe Su,b Zili Zhang,a Chengkui Yang,a Zhilin Hu,a Jue Hou,a Jinying Zhou,a Ling Gong,a Xuejun Jiang,c Chunfu Zheng,b,d Sudan Hea Virus - Publications including interactions involving viral proteins zheng.alan@hotmail.com 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Xiaoliang Yu, Yun Li,a Qin Chen,a Chenhe Su,b Zili Zhang,a Chengkui Yang,a Zhilin Hu,a Jue Hou,a Jinying Zhou,a Ling Gong,a Xuejun Jiang,c Chunfu Zheng,b,d Sudan Hea, Xiaoliang Yu, Yun Li,a Qin Chen,a Chenhe Su,b Zili Zhang,a Chengkui Yang,a Zhilin Hu,a Jue Hou,a Jinying Zhou,a Ling Gong,a Xuejun Jiang,c Chunfu Zheng,b,d Sudan Hea Virus - Publications including interactions involving viral proteins zheng.alan@hotmail.com Accepted 2016-AUG-23 AT 11:53 BST AT 11:53 BST by MGT journal of virology 2015 imex curation Herpes Simplex Virus 1 (HSV-1) and HSV-2 Mediate Species-Specific Modulations of Programmed Necrosis through the Viral Ribonucleotide Reductase Large Subunit R1 Herpes Simplex Virus 1 (HSV-1) and HSV-2 Mediate Species-Specific Modulations of Programmed Necrosis through the Viral Ribonucleotide Reductase Large Subunit R1 journal of virology 2015 imex curation Xiaoliang Yu, Yun Li,a Qin Chen,a Chenhe Su,b Zili Zhang,a Chengkui Yang,a Zhilin Hu,a Jue Hou,a Jinying Zhou,a Ling Gong,a Xuejun Jiang,c Chunfu Zheng,b,d Sudan Hea, Xiaoliang Yu, Yun Li,a Qin Chen,a Chenhe Su,b Zili Zhang,a Chengkui Yang,a Zhilin Hu,a Jue Hou,a Jinying Zhou,a Ling Gong,a Xuejun Jiang,c Chunfu Zheng,b,d Sudan Hea Virus - Publications including interactions involving viral proteins zheng.alan@hotmail.com 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Xiaoliang Yu, Yun Li,a Qin Chen,a Chenhe Su,b Zili Zhang,a Chengkui Yang,a Zhilin Hu,a Jue Hou,a Jinying Zhou,a Ling Gong,a Xuejun Jiang,c Chunfu Zheng,b,d Sudan Hea, Xiaoliang Yu, Yun Li,a Qin Chen,a Chenhe Su,b Zili Zhang,a Chengkui Yang,a Zhilin Hu,a Jue Hou,a Jinying Zhou,a Ling Gong,a Xuejun Jiang,c Chunfu Zheng,b,d Sudan Hea Virus - Publications including interactions involving viral proteins zheng.alan@hotmail.com Accepted 2016-AUG-23 AT 11:53 BST AT 11:53 BST by MGT journal of virology 2015 imex curation ripk3_mouse Receptor-interacting serine/threonine-protein kinase 3 Ripk3 Rip3 RIP-like protein kinase 3 Receptor-interacting protein 3 protein protein mouse Mus musculus Mouse MSSVKLWPTGASAVPLVSREELKKLEFVGKGGFGVVFRAHHRTWNHDVAVKIVNSKKISWEVKAMVNLRNENVLLLLGVTEDLQWDFVSGQALVTRFMENGSLAGLLQPECPRPWPLLCRLLQEVVLGMCYLHSLNPPLLHRDLKPSNILLDPELHAKLADFGLSTFQGGSQSGSGSGSGSRDSGGTLAYLDPELLFDVNLKASKASDVYSFGILVWAVLAGREAELVDKTSLIRETVCDRQSRPPLTELPPGSPETPGLEKLKELMIHCWGSQSENRPSFQDCEPKTNEVYNLVKDKVDAAVSEVKHYLSQHRSSGRNLSAREPSQRGTEMDCPRETMVSKMLDRLHLEEPSGPVPGKCPERQAQDTSVGPATPARTSSDPVAGTPQIPHTLPFRGTTPGPVFTETPGPHPQRNQGDGRHGTPWYPWTPPNPMTGPPALVFNNCSEVQIGNYNSLVAPPRTTASSSAKYDQAQFGRGRGWQPFHK DIP protein Q9QZL0 original sequence version: 88 DIP protein Q9QZL0 original sequence version: 125 DIP protein Q9QZL0 original sequence version: 133 31C5EEE77F55778B u5tqe9_hhv1 Ribonucleoside-diphosphate reductase large subunit RIR1 Ribonucleotide reductase large subunit UL39 protein protein hhv1 Human herpesvirus 1 HHV-1 Human herpes simplex virus 1 MASRPAASSPVEARAPVGGQEAGGPSAATQGEAAGAPLAHGHHVYCQRVNGVMVLSDKTPGSASYRISDSNFVQCGSNCTMIIDGDVVRGRPQDPGAAASPAPFVAVTNIGAGSDGGTAVAAFGGTPRRSAGTSTGTQTADVPAEALGGPPPPPRFTLGGGCCSCRDTRRRSAVFGGEGDPVGPAEFVSDDRSSDSDSDDSDDTDSETLSHASSDVSGGATYDDALDSDSSSDDSLQIDGPVCRPWSNDTAPLDVCPGTPGPGADAGGPSAVDPHAPTPEAGAGLAADPAVARDDAEGLSDPRPRLGTGTAYPVPLELTPENAEAMARFLGDAVNREPALMLEYFCRCAREETKRVPPRTFCSPHRLTEDDFGLLNYALVEMQRLCLDVPPVPPNAYMPYYLREYVTRLVNGFKPLVSRSARLYRILGVLVHLRIRTREASFEEWLRSKEVALDFGLTERLREHEAQLVILAQALDHYDCLIHSTPHTLVERGLQSALKYEEFYLKRFGGHYMESVFQMYTRIAGFLACRATRGMRHIALGREGSWWEMFKFFFHRLYDHQIVPSTPAMLNLGTRNYYTSSCYLVNPQATTNKATLRAITSNVSAILARNGGIGLCVQAFNDSGPGTASVMPALKVLDSLVAAHNKESARPTGACVYLEPWHTDVRAVLRMKGVLAGEEAQRCDNIFSALWMPDLFFKRLIRHLDGEKNVTWTLFDRDTSMSLADFHGEEFEKLYQHLEVMGFGEQIPIQELAYGIVRSAATTGSPFVMFKDAVNRHYIYDTQGAAIAGSNLCTEIVHPASKRSSGVCNLGSVNLARCVSRQTFDFGRLRDAVQACVLMVNIMIDSTLQPTPQCTRGNDNLRSMGIGMQGLHTACLKLGLDLESAEFQDLNKHIAEVMLLSAMKTSNALCVRGARPFNHFKRSMYRAGRFHWERFPDARPRYEGEWEMLRQSMMKHGLRNSQFIALMPTAASAQISDVSEGFAPLFTNLFSKVTRDGETLRPNTLLLKELERTFSGKRLLEVMDSLDAKQWSVAQALPCLEPTHPLRRFKTAFDYDQKLLIDLCADRAPYVDHSQSMTLYVTEKADGTLPASTLVRLLVHAYKRGLKTGMYYCKVRKATNSGVFGGDDNIVCTSCAL 25F310FB1FB9F8D2 ripk1_human Receptor-interacting serine/threonine-protein kinase 1 RIPK1 RIP Cell death protein RIP RIP1 Receptor-interacting protein 1 protein protein human Homo sapiens Human MQPDMSLNVIKMKSSDFLESAELDSGGFGKVSLCFHRTQGLMIMKTVYKGPNCIEHNEALLEEAKMMNRLRHSRVVKLLGVIIEEGKYSLVMEYMEKGNLMHVLKAEMSTPLSVKGRIILEIIEGMCYLHGKGVIHKDLKPENILVDNDFHIKIADLGLASFKMWSKLNNEEHNELREVDGTAKKNGGTLYYMAPEHLNDVNAKPTEKSDVYSFAVVLWAIFANKEPYENAICEQQLIMCIKSGNRPDVDDITEYCPREIISLMKLCWEANPEARPTFPGIEEKFRPFYLSQLEESVEEDVKSLKKEYSNENAVVKRMQSLQLDCVAVPSSRSNSATEQPGSLHSSQGLGMGPVEESWFAPSLEHPQEENEPSLQSKLQDEANYHLYGSRMDRQTKQQPRQNVAYNREEERRRRVSHDPFAQQRPYENFQNTEGKGTAYSSAASHGNAVHQPSGLTSQPQVLYQNNGLYSSHGFGTRPLDPGTAGPRVWYRPIPSHMPSLHNIPVPETNYLGNTPTMPFSSLPPTDESIKYTIYNSTGIQIGAYNYMEIGGTSSSLLDSTNTNFKEEPAAKYQAIFDNTTSLTDKHLDPIRENLGKHWKNCARKLGFTQSQIDEIDHDYERDGLKEKVYQMLQKWVMREGIKGATVGKLAQALHQCSRIDLLSSLIYVSQN DIP protein Q13546 original sequence version: 158 DIP protein Q13546-1 original sequence version: 140 DIP protein Q13546 original sequence version: 146 DIP protein Q13546-1 original sequence version: 146 DIP protein Q13546 original sequence version: 134 DIP protein Q13546 original sequence version: 109 976E2428D525A9B2 rir1_hhv2h Ribonucleoside-diphosphate reductase large subunit RIR1 Ribonucleotide reductase large subunit UL39 protein protein hhv2h Human herpesvirus 2 (strain HG52) (HHV-2) Human herpes simplex virus 2 MANRPAASALAGARSPSERQEPREPEVAPPGGDHVFCRKVSGVMVLSSDPPGPAAYRISDSSFVQCGSNCSMIIDGDVARGHLRDLEGATSTGAFVAISNVAAGGDGRTAVVALGGTSGPSATTSVGTQTSGEFLHGNPRTPEPQGPQAVPPPPPPPFPWGHECCARRDARGGAEKDVGAAESWSDGPSSDSETEDSDSSDEDTGSETLSRSSSIWAAGATDDDDSDSDSRSDDSVQPDVVVRRRWSDGPAPVAFPKPRRPGDSPGNPGLGAGTGPGSATDPRASADSDSAAHAAAPQADVAPVLDSQPTVGTDPGYPVPLELTPENAEAVARFLGDAVDREPALMLEYFCRCAREESKRVPPRTFGSAPRLTEDDFGLLNYALAEMRRLCLDLPPVPPNAYTPYHLREYATRLVNGFKPLVRRSARLYRILGVLVHLRIRTREASFEEWMRSKEVDLDFGLTERLREHEAQLMILAQALNPYDCLIHSTPNTLVERGLQSALKYEEFYLKRFGGHYMESVFQMYTRIAGFLACRATRGMRHIALGRQGSWWEMFKFFFHRLYDHQIVPSTPAMLNLGTRNYYTSSCYLVNPQATTNQATLRAITGNVSAILARNGGIGLCMQAFNDASPGTASIMPALKVLDSLVAAHNKQSTRPTGACVYLEPWHSDVRAVLRMKGVLAGEEAQRCDNIFSALWMPDLFFKRLIRHLDGEKNVTWSLFDRDTSMSLADFHGEEFEKLYEHLEAMGFGETIPIQDLAYAIVRSAATTGSPFIMFKDAVNRHYIYDTQGAAIAGSNLCTEIVHPASKRSSGVCNLGSVNLARCVSRQTFDFGRLRDAVQACVLMVNIMIDSTLQPTPQCTRGNDNLRSMGIGMQGLHTACLKMGLDLESAEFRDLNTHIAEVMLLAAMKTSNALCVRGARPFSHFKRSMYRAGRFHWERFSNASPRYEGEWEMLRQSMMKHGLRNSQFIALMPTAASAQISDVSEGFAPLFTNLFSKVTRDGETLRPNTLLLKELERTFGGKRLLDAMDGLEAKQWSVAQALPCLDPAHPLRRFKTAFDYDQELLIDLCADRAPYVDHSQSMTLYVTEKADGTLPASTLVRLLVHAYKRGLKTGMYYCKVRKATNSGVFAGDDNIVCTSCAL 5A46D3985338BB21 mlkl_mouse Mixed lineage kinase domain-like protein Mlkl protein protein mouse Mus musculus Mouse MDKLGQIIKLGQLIYEQCEKMKYCRKQCQRLGNRVHGLLQPLQRLQAQGKKNLPDDITAALGRFDEVLKEANQQIEKFSKKSHIWKFVSVGNDKILFHEVNEKLRDVWEELLLLLQVYHWNTVSDVSQPASWQQEDRQDAEEDGNENMKVILMQLQISVEEINKTLKQCSLKPTQEIPQDLQIKEIPKEHLGPPWTKLKTSKMSTIYRGEYHRSPVTIKVFNNPQAESVGIVRFTFNDEIKTMKKFDSPNILRIFGICIDQTVKPPEFSIVMEYCELGTLRELLDREKDLTMSVRSLLVLRAARGLYRLHHSETLHRNISSSSFLVAGGYQVKLAGFELSKTQNSISRTAKSTKAERSSSTIYVSPERLKNPFCLYDIKAEIYSFGIVLWEIATGKIPFEGCDSKKIRELVAEDKKQEPVGQDCPELLREIINECRAHEPSQRPSVDGRSLSGRERILERLSAVEESTDKKV 75E0E3E3DDB5DFB2 ripk3_human Receptor-interacting serine/threonine-protein kinase 3 RIPK3 RIP3 RIP-like protein kinase 3 Receptor-interacting protein 3 protein protein human Homo sapiens Human MSCVKLWPSGAPAPLVSIEELENQELVGKGGFGTVFRAQHRKWGYDVAVKIVNSKAISREVKAMASLDNEFVLRLEGVIEKVNWDQDPKPALVTKFMENGSLSGLLQSQCPRPWPLLCRLLKEVVLGMFYLHDQNPVLLHRDLKPSNVLLDPELHVKLADFGLSTFQGGSQSGTGSGEPGGTLGYLAPELFVNVNRKASTASDVYSFGILMWAVLAGREVELPTEPSLVYEAVCNRQNRPSLAELPQAGPETPGLEGLKELMQLCWSSEPKDRPSFQECLPKTDEVFQMVENNMNAAVSTVKDFLSQLRSSNRRFSIPESGQGGTEMDGFRRTIENQHSRNDVMVSEWLNKLNLEEPPSSVPKKCPSLTKRSRAQEEQVPQAWTAGTSSDSMAQPPQTPETSTFRNQMPSPTSTGTPSPGPRGNQGAERQGMNWSCRTPEPNPVTGRPLVNIYNCSGVQVGDNNYLTMQQTTALPTWGLAPSGKGRGLQHPPPVGSQEGPKDPEAWSRPQGWYNHSGK DIP protein Q9Y572 original sequence version: 134 BF755F8A0B1810A1 icp6-rip3-1 1 5 unspecified role unspecified role bait bait rhim sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined U5TQE9:p.Val73_Gly76delinsAlaAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain VQCG AAAA Mon Oct 03 14:53:27 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val73ala_gln74ala_cys75ala_gly76ala' Wed May 02 16:25:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_gln_cys_gly73-76ala_ala_ala_ala' Tue Jun 30 16:47:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val73_Gly76delinsAlaAlaAlaAla' 9 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4E icp10-ripk3-1 1 7 unspecified role unspecified role bait bait P89462:p.Val64_Gly67delinsAlaAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain VQCG AAAA Fri Jul 22 10:52:37 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val64ala_gln65ala_cys66ala_gly67ala' Wed May 02 20:03:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_gln_cys_gly64-67ala_ala_ala_ala' Tue Jun 30 19:39:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val64_Gly67delinsAlaAlaAlaAla' flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5A icp6-rip1-1 1 7 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5B rip1-rip3-1 1 9 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 inhibitor inhibitor unspecified role unspecified role myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 5c ripk1-icp6-1 1 5 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5C ripk1-ul39-1 1 5 unspecified role unspecified role prey prey U5TQE9:p.Val73_Gly76delinsAlaAlaAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain VQCG AAAA Thu Dec 08 14:31:48 GMT 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val_gln_cys_gly64-67ala_ala_ala_ala' Wed May 02 19:39:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_gln_cys_gly73-76ala_ala_ala_ala' Tue Jun 30 18:53:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val73_Gly76delinsAlaAlaAlaAla' myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5B mlkl-rip-1 3 4 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1E mlkl-ripk3-3 3 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 1E ripk3-ripk1-5 2 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3Di TNF-alpha/Smac mimetic/Z-VAD HSV1 and 2 infection disrupted this interaction;deletion of ICP6 (HSV1) allowed the interaction to occur.