HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus. Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus. Journal of virology 2015 imex curation Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Accepted 2016-DEC-06 AT 12:30 GMT AT 12:30 GMT by MGT Journal of virology 2015 imex curation Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus. Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus. Journal of virology 2015 imex curation Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Accepted 2016-DEC-06 AT 12:30 GMT AT 12:30 GMT by MGT Journal of virology 2015 imex curation Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus. Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus. Journal of virology 2015 imex curation Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Accepted 2016-DEC-06 AT 12:30 GMT AT 12:30 GMT by MGT Porcine primary macrophage cDNA library Journal of virology 2015 imex curation Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus. Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus. Journal of virology 2015 imex curation Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Accepted 2016-DEC-06 AT 12:30 GMT AT 12:30 GMT by MGT Journal of virology 2015 imex curation Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus. Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus. Journal of virology 2015 imex curation Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation pig-pk15 pig pk15 Pig pig-pk15 pig kidney pk15 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Accepted 2016-DEC-06 AT 12:30 GMT AT 12:30 GMT by MGT Journal of virology 2015 imex curation Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus. Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus. Journal of virology 2015 imex curation Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation pig-pk15 pig pk15 Pig pig-pk15 pig kidney pk15 confocal microscopy confocal microscopy immunostaining immunostaining Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Accepted 2016-DEC-06 AT 12:30 GMT AT 12:30 GMT by MGT Journal of virology 2015 imex curation k7zla6_pig IGHG3 protein protein pig Sus scrofa (Pig) Pig YNTAPSVYPLAPCGRDVSDHNVALGCLVSSYFPEPVTVTWNSGALSRVVHTFPSVLQPSGLYSLSSMVIVAASSLSTLSYTCNVYHPATNTKVDKRVDIEPPTPICPEICSCPAAEVLGAPSVFLFPPKPKDILMISRTPKVTCVVVDVSQEEAEVQFSWYVDGVQLYTAQTRPMEEQFNSTYRVVSVLPIQHQDWLKGKEFKCKVNNKDLLSPITRTISKATGPSRVPQVYTLPPAWEELSKSKVSITCLVTGFYPPDIDVEWQSNGQQEPEGNYRTTPPQQDVDGTYFLYSKLAVDKVRWQRGDLFQCAVMHEALHNHYTQKSVSQTPGK C71DE2E866E69A30 i3lii3_pig EEF2 protein protein pig Sus scrofa (Pig) Pig MAKGKESKTLVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGSGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGPAERAKKVEDMMKKLWGDRYFDPANGKFSKSATSPDGKKLPRTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNTSRPSQVVAETRKRKGLKEGIPALDNFLDKL A287FEB393D40E72 q5u8x5_9flav Autoprotease p20 Capsid protein C Cysteine protease NS2 E(rns) glycoprotein Envelope glycoprotein E1 Envelope glycoprotein E2 Genome polyprotein N-terminal protease NS5B Non-structural protein 2 Non-structural protein 2-3 Non-structural protein 3 Non-structural protein 4A Non-structural protein 4B Non-structural protein 5A RNA-directed RNA polymerase Serine protease NS3 gp33 gp44/48 gp55 Viroporin p7 protein protein classical swine fever virus Classical swine fever virus MELNHFELLYKTNKQKPMGVEEPVYDVTGRPLFGDPSEVHPQSTLKLPHDRGRGNIKTTLNNLPRKGDCRSGNHLGPVSGIYVKPGPVFYQDYMGPVYHRAPLEFFNEAQFCEVTKRIGRVTGSDGKLYHIYVCIDGCILLKLAKRGEPRTLKWIRNFTDCPLWVTSCSDDGASGSKEKKPDRINKGKLKIAPKEHEKDSRTKPPDATIVVEGVKYQVKKKGKVKGKNTQDGLYHNKNKPPESRKKLEKALLAWAVITIMLYQPVEAENITQWNLSDNGTNGIQHAMYLRGVSRSLHGIWPEKICKGVPTYLATDTELKEIQGMMDASEGTNYTCCKLQRHEWNKHGWCNWYNIDPWIQLMNRTQANLAEGPPAKECAVTCRYDKDADINVVTQARNRPTTLTGCKKGKNFSFAGTIIEGPCNFNVSVEDILYGDHECGSLLQDTALYLVDGMTNTIENARQGAARVTSWLGRQLSTAGKRLEGRSKTWFGAYALSPYCNVTSKIGYIWYTNNCTPACLPKNTKIIGPGKFDTNAEDGKILHEMGGHLSEFLLLSLVVLSDFAPETASALYLILHYVIPQSYEEPEGCDTNQLNLTVELRTEDVIPSSVWNVGKYVCVRPDWWPYETKVALLFEEAGQVVKLALRALRDLTRVWNSASTTAFLICLIKVLRGQIVQGVIWLLLVTGAQGRLACKEDYRYALSSTNEIGLLGAGGLTTTWEEYSHDLQLNDGTVKAICVAGSFKVTALNVVSRRYLASLHKGALLTSVTFELLFDGTNPSTEEMGDDFGFGLCPFDTSPVVKGKYNTTLLNGSAFYLVCPIGWTGVIECTAVSPTTLRTEVVKTFRREKPFPHRMDCVTTTVENEDLFYCKLGGNWTCVKGEPVVYTGGQVKQCKWCGFDFNEPDGLPHYPIGKCILANETGYRIVDSTDCNRDGVVISAEGSHECLIGNTTVKVHASDERLGPMPCRPKEIVSSAGPVRKTSCTFNYAKTLKNKYYEPRDSYFQQYMLKGEYQYWFDLDVTDRHSDYFAEFVVLVVVALLGGRYVLWLIVTYIVLTEQLAAGLPLGQGEVVLIGNLITHTDIEVVVYFLLLYLVMRDEPIKKWILLLFHAMTNNPVKTITVALLMVSGVAKGGKIDGGWQRLPETSFDIQLALTVIVVAVMLLAKRDPTTVPLVITVATLRTAKMTNGLSTDIAIATVSTALLTWTYISDYYRYKTWLQYLISTVTGIFLIRVLKGIGELDLHTPTLPSYRPLFFILVYLISTAVVTRWNLDIAGLLLQCVPTLLMVFTMWADILTLILILPTYELTKLYYLKEVKIGAERGWLWKTNFKRVNDIYEVDQAGEGVYLFPSKQKTSSITGTMLPLIKAILISCISNKWQFIYLLYLIFEVSYYLHKKIIDEIAGGTNFISRLVAALIEANWAFDNEEVRGLKKFFLLSSRVKELIIKHKVRNEVMVHWFGDEEVYGMPKLVGLVKAATLSKNKHCILCTVCEDREWKGETCPKCGRFGPPMTCGMTLADFEEKHYKRIFFREDQSEGPVREEYAGYLQYRARGQLFLRNLPVLATKVKMLLVGNLGTEVGDLEHLGWVLRGPAVCKKVTEHEKCTTSIMDKLTAFFGVMPRGTTPRAPVRFPTSLLKIRRGLETGWAYTHQGGISSVDHVTCGKDLLVCDTMGRTRVVCQSNNKMTDESEYGVKTDSGCPEGARCYVFNPEAVNISGTKGAMVHLQKTGGEFTCVTASGTPAFFDLKNLKGWSGLPIFEASSGRVVGRVKVGKNEDSKPTKLMSGIQTVSKSTTDLTEMVKKITTMNRGEFRQITLATGAGKTTELPRSVIEEIGRHKRVLVLIPLRAAAESVYQYMRQKHPSIAFNLRIGEMKEGDMATGITYASYGYFCQMPQPKLRAAMVEYSFIFLDEYHCATPEQLAIMGKIHRFSENLRVVAMTATPAGTVTTTGQKHPIEEFIAPEVMKGEDLGSEYLDIAGLKIPVEEMKSNMLVFVPTRNMAVETAKKLKAKGYNSGYYYSGEDPSNLRVVTSQSPYVVVATNAIESGVTLPDLDVVVDTGLKCEKRIRLSSKMPFIVTGLKRMAVTIGEQAQRRGRVGRVKPGRYYRSQETPVGSKDYHYDLLQAQRYGIEDGINITKSFREMNYDWSLYEEDSLMITQLEILNNLLISEELPMAVKNIMARTDHPEPIQLAYNSYETQVPVLFPKIRNGEVTDSYDNYTFLNARKLGDDVPPYVYATEDEDLAVELLGLDWPDPGNQGTVEAGRALKQVVGLSTAENALLVALFGYVGYQALSKRHIPVVTDIYSIEDHRLEDTTHLQYAPNAIKTEGKETELKELAQGDVQRCVEAMTNYAREGIQFMKSQALKVKETPTYKETMDTVTDYVKKFMEALKDSKEDIMKYGLWGTHTALYKSISARLGSETAFATLVVKWLAFGGESIADHVKQAATDLVVYYIINRPQFPGDTETQQEGRKFVASLLVSALATYTYKSWNYNNLSKIVEPALATLPYAATALKLFAPTRLESVVILSTAIYKTYLSIRRGKSDGLLGTGVSAAMEIMSQNPVSVGIAVMLGVGAVAAHNAIEASEQKRTLLMKVFVKNFLDQAATDELVKESPEKIIMALFEAVQTVGNPLRLVYHLYGVFYKGWEAKELAQRTAGRNLFTLIMFEAVELLGVDSEGKIRQLSSNYILELLYKFRDSIKSSVREMAISWAPAPFSCDWTPTDDRIGLPQDNFLQVETKCPCGYKMKAVKNCAGELRLLEEEGSFLCRNKFGRGSRNYKVTKYYDDNLSEIKPVIKMEGHVELYYKGATIKLDFNNSKTILATDKWEVDHSTLVRVLKRHTGAGYNGAYLGEKPNHKHLIERDCATITKDKVCFLKMKRGCAFTYDLSLHNLTRLIELVHKNNLEDKEIPAVTVTTWLAYTFVNEDIGTIRPAFGEKVTPEMQEEITLQPAVVVDTTDVTVTVVGEAPTMTTGETPTAFTSSGSDPKGQQVLKLGVGEGQYPGTNPQRASLHEAIQGADERPSVLILGSDKATSNRVKTAKNVKVYRGRDPLEVRDMMRRGKILVIALSRVDNALLKFVDYKGTFLTRETLEALSLGRPKKKDITKAEAQWLLCLEDQMEELPDWFAAGEPIFLEANIKHDRYHLVGDIATIKEKAKQLGATDSTKISKEVGAKVYSMKLSNWVMQEENKQGNLTPLFEELLQQCPPGGQNKTAHMVSAYQLAQGNWMPTSCHVFMGTISARRTKTHPYEAYVKLRELVEEHKMKTLCPGSSLGKHNDWIIGKIKYQGNLRTKHMLNPGKVAEQLCREGHRHNVYNKTIGSVMTATGIRLEKLPVVRAQTDTTNFHQAIRDKIDKEENLQTPGLHKKLMEVFNALKRPELESSYDAVEWEELERGINRKGAAGFFERKNIGEILDSEKNKVEEIIDNLKKGRNIKYYETAIPKNEKRDVNDDWTAGDFVDEKKPRVIQYPEAKTRLAITKVMYKWVKQKPVVIPGYEGKTPLFQIFDKVKKEWDQFQNPVAVSFDTKAWDTQVTTKDLELIKDIQKYYFKKKWHKFIDTLTMHMTEVPVICADGEVYIRKGQRGSGQPDTSAGNSMLNVLTMVYAFCEATGVPYKSFDRVAKIHVCGDDGFLITERALGEKFASKGVQILYEAGKPQKITEGDKMKVAYQFDDIEFCSHTPIQVRWSDNTSSYMPGRNTTTILAKMATRLDSSGERGTIAYEKAVAFSFLLMYSWNPLIRRICLLVLSTELQVKPGKSTTYYYEGDPISAYKEVIGHNLFDLKRTSFEKLAKLNLSMSVLGAWTRHTSKRLLQDCVNMGVKEGNWLVNADRLVSSKTGNRYIPGEGHTLQGRHYEELVLARKQINNFQGTDRYNLGPIVNMVLRRLRVMMMTLIGRGV 221D3B27E69E8E44 f1skj2_pig TXN2 protein protein pig Sus scrofa (Pig) Pig MVRSGIAAEAPLQARSHAGRWPSGQKPWMAQRLLLRRFLASVVSRKPSHSRWAPLASRALQTPQLSLSNLTGTPSQARSLYTTQVCTTTFNIQDGPDFQDRVVNSETPVVVDFHAQWCGPCKILGPRLEKVVAKQHGKVVMAKVDIDDHTDLAIEYEVSAVPTVLAIKNGDVVDKFVGIKDEDQLEAFLKKLIG BF4777BB41E5BE91 igll5_pig Immunoglobulin lambda-like polypeptide 5 protein protein pig Sus scrofa (Pig) Pig similar to B9A064 qki_pig Protein quaking QKI protein protein pig Sus scrofa (Pig) Pig MVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERLLDEEISRVRKDMYNDTLNGSTEKRSAELPDAVGPIVQLQEKLYVPVKEYPDFNFVGRILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDAQNRAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSLAATAQAAPRIITGPAPVLPPAALRTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVPPGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVLGAVATKVRRHDMRVHPYQRIVTADRAATGN 43E7F3A426A494C4 f1s3w8_pig CYP4B1 protein protein pig Sus scrofa (Pig) Pig MVPALLSLSLSHLSLWALGLILALGVLKLTSLLLRRQMLARAMDKFPGPPTHWIFGHALEIQQTGSLDKMVSWAHQFPHAHPLWLGPFLGFLNIYEPEYAKAVYSRGGEGSRERWDLLRAEGKGLLVLHGPKWFQHRKLLTPGFHHDVLKPYVAVMANSARVMLDKWEEKAREDKSFDIYHDVGHMALELLMKCTFGKGTSGLNYSDNAYHLAVRDLTLLMQQRLSSFQYHNDFIYWLTPHGRRFLRACRVAHDHTDQVIRERKAALQDKEEQERIQSRRHLDFLDILLGAQDEDRVKLSDADLRAEVDTFMFGGHDTTTRGISWFLYCMALNPEHQHRCREEIREILGDRDSIQGEDLGKMTYLTMCIRESFRLYPPVPQVYRQLSEPVSFVDGRSLPAGSLISLHIYALHRNSAVWPDPEVFDPLRFSLENMAGRHPFAFLPFSAGPRNCIGQRLP 0536D2AB88A611E3 rack1_pig Receptor of activated protein C kinase 1 RACK1 GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Receptor for activated C kinase Receptor of activated protein kinase C 1 protein protein pig Sus scrofa (Pig) Pig MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR 257F91E369ED2044 a3ex84_pig Galectin LGALS3 protein protein pig Sus scrofa (Pig) Pig MADGFSLNDALSGSGKPNPQGWPGAWGNQPAGPGGYPGASYPGTYPGQGPPGAYPGQAPPGAYPGQAPPGAYPGPTAPGYPGPAAPGAYPGQPGGPGAYPPPAQPSAPGAYPATGPYGAPSGPLNVPYDLPLPGGVMPRMLITILGTVKPNANRLALDFKKGNDVAFHFNPRFNEDNRRVIVCNSKLDNNWGREERQMVFPFECGKPFKIQVLVEPDHFKVAVNDAHLLQYNHRMRNLREISKLGISGDITLTSASHTMI 6B5C17C0D5AE9E9D q5u8x5_9flav-1 3 9 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role prey prey physical association physical association table 2 q5u8x5_9flav-ighg3-1 3 9 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey physical association physical association table 2 q5u8x5_9flav-rack1-1 3 9 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 14 unspecified role unspecified role prey prey physical association physical association table 2 q5u8x5_9flav-cyp4b1-1 3 9 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role prey prey physical association physical association table 2 q5u8x5_9flav-qki-1 3 9 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role prey prey physical association physical association table 2 q5u8x5_9flav-eef2-1 3 9 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey physical association physical association table 2 q5u8x5_9flav-txn-1 3 9 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey physical association physical association table 2 q5u8x5_9flav-igll5_pig-1 3 9 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role prey prey physical association physical association table 2 e2-trx2-1 1 9 unspecified role unspecified role bait bait c terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range e2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q5U8X5:p.Asn705Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N D Wed May 02 16:09:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn705asp' Tue Jun 30 16:34:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn705Asp' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 3C A chimeric E2 mutants between Shimen strain (UniProt id Q5U8X5) and C-strain (UniProt id O92364) is used in the study txn-q5u8x5_9flav-1 1 9 unspecified role unspecified role prey prey c terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range e2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 1B, 2 e2-trx2-2 1 9 unspecified role unspecified role bait bait e2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 1C e2-txn2-1 2 9 unspecified role unspecified role neutral component neutral component gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined e2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role neutral component neutral component mcherry mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 1D q5u8x5_9flav-txn2-1 5 9 unspecified role unspecified role neutral component neutral component c terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range e2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role neutral component neutral component myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction 1E q5u8x5_9flav-txn2-2 4 9 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey physical association physical association Fig 1A q5u8x5_9flav-txn2-3 6 9 unspecified role unspecified role neutral component neutral component e2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role neutral component neutral component colocalization colocalization 1D q5u8x5_9flav-txn2-4 1 9 unspecified role unspecified role prey prey c terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range e2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 10 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 3C