IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes. Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes. The Journal of biological chemistry 2014 imex curation Bouvet M., Lugari A., Posthuma CC., Zevenhoven JC., Bernard S., Betzi S., Imbert I., Canard B., Guillemot JC., Lécine P., Pfefferle S., Drosten C., Snijder EJ., Decroly E., Morelli X. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus xavier.morelli@inserm.fr Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret tag fluorescence tag visualisation by fluorescence tag fluorescence Bouvet M., Lugari A., Posthuma CC., Zevenhoven JC., Bernard S., Betzi S., Imbert I., Canard B., Guillemot JC., Lécine P., Pfefferle S., Drosten C., Snijder EJ., Decroly E., Morelli X. Virus - Publications including interactions involving viral proteins xavier.morelli@inserm.fr Accepted 2020-JUN-11 AT 14:41 BST AT 14:41 BST by MARTA The Journal of biological chemistry 2014 Only protein-protein interactions imex curation imex curation Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes. Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes. The Journal of biological chemistry 2014 imex curation Bouvet M., Lugari A., Posthuma CC., Zevenhoven JC., Bernard S., Betzi S., Imbert I., Canard B., Guillemot JC., Lécine P., Pfefferle S., Drosten C., Snijder EJ., Decroly E., Morelli X. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus xavier.morelli@inserm.fr Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret anti tag western anti tag western blot anti tag western Bouvet M., Lugari A., Posthuma CC., Zevenhoven JC., Bernard S., Betzi S., Imbert I., Canard B., Guillemot JC., Lécine P., Pfefferle S., Drosten C., Snijder EJ., Decroly E., Morelli X. Virus - Publications including interactions involving viral proteins xavier.morelli@inserm.fr Accepted 2020-JUN-11 AT 14:41 BST AT 14:41 BST by MARTA The Journal of biological chemistry 2014 Only protein-protein interactions imex curation imex curation Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes. Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes. The Journal of biological chemistry 2014 imex curation Bouvet M., Lugari A., Posthuma CC., Zevenhoven JC., Bernard S., Betzi S., Imbert I., Canard B., Guillemot JC., Lécine P., Pfefferle S., Drosten C., Snijder EJ., Decroly E., Morelli X. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus xavier.morelli@inserm.fr Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight identificat confirmation by molecular weight weight identificat Bouvet M., Lugari A., Posthuma CC., Zevenhoven JC., Bernard S., Betzi S., Imbert I., Canard B., Guillemot JC., Lécine P., Pfefferle S., Drosten C., Snijder EJ., Decroly E., Morelli X. Virus - Publications including interactions involving viral proteins xavier.morelli@inserm.fr Accepted 2020-JUN-11 AT 14:41 BST AT 14:41 BST by MARTA The Journal of biological chemistry 2014 Only protein-protein interactions imex curation imex curation p0c6x7-pro_0000037320 Proofreading exoribonuclease nsp14 rep ORF1ab polyprotein 1a-1b protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus AENVTGLFKDCSKIITGLHPTQAPTHLSVDIKFKTEGLCVDIPGIPKDMTYRRLISMMGFKMNYQVNGYPNMFITREEAIRHVRAWIGFDVEGCHATRDAVGTNLPLQLGFSTGVNLVAVPTGYVDTENNTEFTRVNAKPPPGDQFKHLIPLMYKGLPWNVVRIKIVQMLSDTLKGLSDRVVFVLWAHGFELTSMKYFVKIGPERTCCLCDKRATCFSTSSDTYACWNHSVGFDYVYNPFMIDVQQWGFTGNLQSNHDQHCQVHGNAHVASCDAIMTRCLAVHECFVKRVDWSVEYPIIGDELRVNSACRKVQHMVVKSALLADKFPVLHDIGNPKAIKCVPQAEVEWKFYDAQPCSDKAYKIEELFYSYATHHDKFTDGVCLFWNCNVDRYPANAIVCRFDTRVLSNLNLPGCDGGSLYVNKHAFHTPAFDKSAFTNLKQLPFFYYSDSPCESHGKQVVSDIDYVPLKSATCITRCNLGGAVCRHHANEYRQYLDAYNMMISAGFSLWIYKQFDTYNLWNTFTRLQ 5903 6429 D8E25737EF40967D p0c6x7-pro_0000037317 Non-structural protein 10 rep ORF1ab polyprotein 1a-1b protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus AGNATEVPANSTVLSFCAFAVDPAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLRNTVCTVCGMWKGYGCSCDQLREPLMQ 4231 4369 1F48DD3A6874826C rep-rep-68 1 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell P0C6X7-PRO_0000037317:p.Ala61Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A V Tue Jun 30 15:44:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala61Val' region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0C6X7-PRO_0000037317:p.Cys73Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 16:04:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys73Arg' P0C6X7-PRO_0000037317:p.Arg78Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R G Tue Jun 30 18:51:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg78Gly' P0C6X7-PRO_0000037317:p.His83Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain H R Tue Jun 30 18:10:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His83Arg' P0C6X7-PRO_0000037317:p.Lys93Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K E Tue Jun 30 19:20:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys93Glu' P0C6X7-PRO_0000037317:p.Gly94Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 18:14:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Asp' P0C6X7-PRO_0000037317:p.Cys120Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 19:05:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys120Arg' P0C6X7-PRO_0000037317:p.Val42Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V A Tue Jun 30 19:14:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val42Ala' P0C6X7-PRO_0000037317:p.Met44Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain M A Tue Jun 30 18:13:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met44Ala' P0C6X7-PRO_0000037317:p.Gly70Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 18:04:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly70Ala' P0C6X7-PRO_0000037317:p.Ser72Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 19:58:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser72Ala' P0C6X7-PRO_0000037317:p.Arg78Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 20:18:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg78Ala' P0C6X7-PRO_0000037317:p.Lys93Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 16:44:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys93Ala' P0C6X7-PRO_0000037317:p.Gly94Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 18:32:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Ala' P0C6X7-PRO_0000037317:p.Lys95Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 18:19:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys95Ala' P0C6X7-PRO_0000037317:p.Tyr96Phe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y F Tue Jun 30 17:36:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr96Phe' P0C6X7-PRO_0000037317:p.Tyr96Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 15:44:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr96Ala' P0C6X7-PRO_0000037317:p.Tyr96Ile mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y I Tue Jun 30 19:53:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr96Ile' 4 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell region renilla luciferase renilla luciferase protein tag renilla luciferase undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction Fig. 2a, Table 1 rep-91 2 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell P0C6X7-PRO_0000037317:p.Ala61Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A V Tue Jun 30 17:28:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala61Val' region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0C6X7-PRO_0000037317:p.Cys73Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 19:27:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys73Arg' P0C6X7-PRO_0000037317:p.Arg78Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R G Tue Jun 30 19:52:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg78Gly' P0C6X7-PRO_0000037317:p.His83Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain H R Tue Jun 30 17:09:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His83Arg' P0C6X7-PRO_0000037317:p.Lys93Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K E Tue Jun 30 16:03:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys93Glu' P0C6X7-PRO_0000037317:p.Gly94Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 18:30:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Asp' P0C6X7-PRO_0000037317:p.Cys120Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 19:26:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys120Arg' P0C6X7-PRO_0000037317:p.Val42Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V A Tue Jun 30 19:34:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val42Ala' P0C6X7-PRO_0000037317:p.Met44Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain M A Tue Jun 30 17:17:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met44Ala' P0C6X7-PRO_0000037317:p.Gly70Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 19:07:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly70Ala' P0C6X7-PRO_0000037317:p.Ser72Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 19:32:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser72Ala' P0C6X7-PRO_0000037317:p.Arg78Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 19:15:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg78Ala' P0C6X7-PRO_0000037317:p.Lys93Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 18:47:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys93Ala' P0C6X7-PRO_0000037317:p.Gly94Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 15:43:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Ala' P0C6X7-PRO_0000037317:p.Lys95Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 19:29:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys95Ala' P0C6X7-PRO_0000037317:p.Tyr96Phe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y F Tue Jun 30 17:20:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr96Phe' P0C6X7-PRO_0000037317:p.Tyr96Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 18:06:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr96Ala' P0C6X7-PRO_0000037317:p.Tyr96Ile mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y I Tue Jun 30 18:25:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr96Ile' 4 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell region renilla luciferase renilla luciferase protein tag renilla luciferase undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction Fig. 2b, Table 1 rep-rep-75 3 5 unspecified role unspecified role bait bait purified purified P0C6X7-PRO_0000037317:p.Ser72Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 19:28:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser72Ala' P0C6X7-PRO_0000037317:p.Arg78Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 18:17:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg78Ala' P0C6X7-PRO_0000037317:p.Tyr126Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 19:49:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr126Ala' P0C6X7-PRO_0000037317:p.Phe19Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Tue Jun 30 18:29:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe19Ala' P0C6X7-PRO_0000037317:p.Asn40Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N A Tue Jun 30 19:58:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn40Ala' P0C6X7-PRO_0000037317:p.Val42Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V A Tue Jun 30 17:51:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val42Ala' P0C6X7-PRO_0000037317:p.Lys43Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 18:09:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys43Ala' P0C6X7-PRO_0000037317:p.Met44Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain M A Tue Jun 30 17:31:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met44Ala' P0C6X7-PRO_0000037317:p.Leu45Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 19:22:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu45Ala' P0C6X7-PRO_0000037317:p.Thr47Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T A Tue Jun 30 17:34:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr47Ala' P0C6X7-PRO_0000037317:p.Thr58Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T A Tue Jun 30 15:34:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr58Ala' P0C6X7-PRO_0000037317:p.Ala61Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A V Tue Jun 30 16:35:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala61Val' P0C6X7-PRO_0000037317:p.Gly69Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 20:06:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly69Ala' P0C6X7-PRO_0000037317:p.Arg78Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R G Tue Jun 30 15:45:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg78Gly' P0C6X7-PRO_0000037317:p.His80Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain H A Tue Jun 30 17:09:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His80Ala' P0C6X7-PRO_0000037317:p.Lys87Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 17:18:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys87Ala' P0C6X7-PRO_0000037317:p.Lys93Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 17:00:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys93Ala' P0C6X7-PRO_0000037317:p.Lys93Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K E Tue Jun 30 16:51:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys93Glu' P0C6X7-PRO_0000037317:p.Gly94Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 17:37:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Ala' P0C6X7-PRO_0000037317:p.Gly94Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 17:28:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Asp' P0C6X7-PRO_0000037317:p.Tyr96Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 19:50:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr96Ala' P0C6X7-PRO_0000037317:p.Tyr96Ile mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y I Tue Jun 30 15:49:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr96Ile' P0C6X7-PRO_0000037317:p.Tyr96Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y V Tue Jun 30 17:45:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr96Val' P0C6X7-PRO_0000037317:p.Tyr96Phe mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y F Tue Jun 30 19:26:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr96Phe' n-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0C6X7-PRO_0000037317:p.Gly70Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 19:45:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly70Ala' ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3a