HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Interactome profile of the host cellular proteins and the nonstructural protein 2 of porcine reproductive and respiratory syndrome virus. Interactome profile of the host cellular proteins and the nonstructural protein 2 of porcine reproductive and respiratory syndrome virus. PloS one 2013 imex curation Wang L., Zhou L., Zhang H., Li Y., Ge X., Guo X., Yu K., Yang H. Virus - Publications including interactions involving viral proteins Results from Fig 3/table S3 are not included due to lack of UniProt identifiers for African Green Monkey proteins. In addition, the high confidence interactors could not be identified. The PRRSV strain used could not be traced, so the reference strain Lelystad has been used instead. yanghanchun1@cau.edu.cn 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang L., Zhou L., Zhang H., Li Y., Ge X., Guo X., Yu K., Yang H. Virus - Publications including interactions involving viral proteins Results from Fig 3/table S3 are not included due to lack of UniProt identifiers for African Green Monkey proteins. In addition, the high confidence interactors could not be identified. The PRRSV strain used could not be traced, so the reference strain Lelystad has been used instead. yanghanchun1@cau.edu.cn Accepted 2016-SEP-21 AT 11:22 BST AT 11:22 BST by MGT PloS one 2013 imex curation Interactome profile of the host cellular proteins and the nonstructural protein 2 of porcine reproductive and respiratory syndrome virus. Interactome profile of the host cellular proteins and the nonstructural protein 2 of porcine reproductive and respiratory syndrome virus. PloS one 2013 imex curation Wang L., Zhou L., Zhang H., Li Y., Ge X., Guo X., Yu K., Yang H. Virus - Publications including interactions involving viral proteins Results from Fig 3/table S3 are not included due to lack of UniProt identifiers for African Green Monkey proteins. In addition, the high confidence interactors could not be identified. The PRRSV strain used could not be traced, so the reference strain Lelystad has been used instead. yanghanchun1@cau.edu.cn 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-marc145 chlae MARC- 145 cells Green monkey Cercopithecus aethiops marc145 African green monkey kidney epithelial cell line MARC- 145 cells Parent cell line is MA-104. The species of origin of the MA104 cell line has been shown to be different from that claimed by the originators. Whitaker & Hayward (1985) found the cell line to originate from an African Green monkey and not a Rhesus macaque as was originally claimed. PMID: 4043530 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wang L., Zhou L., Zhang H., Li Y., Ge X., Guo X., Yu K., Yang H. Virus - Publications including interactions involving viral proteins Results from Fig 3/table S3 are not included due to lack of UniProt identifiers for African Green Monkey proteins. In addition, the high confidence interactors could not be identified. The PRRSV strain used could not be traced, so the reference strain Lelystad has been used instead. yanghanchun1@cau.edu.cn Accepted 2016-SEP-21 AT 11:22 BST AT 11:22 BST by MGT PloS one 2013 imex curation a0a068ca64_pig AIFM1 protein protein pig Sus scrofa (Pig) Pig MFRCGGLAAGALKQKLAPLVRTVCVRGPRQRNRLPGHLFQRWHVPLELQMTRQMASSGASGGKIDNTVLVLFVGLSTIGAGAYAYRTIKDNKKRYNERIAGLGLTPEEKQKRATSSAPEGEPVPQVRVPSHVPFLLIGGGTAAFAAARSIRARDPGARVLIVSEDPELPYMRPPLSKELWFSDDPNVTKTLRFRQWNGKERSIYFQPPSFYVSAQDLPHIENGGVAVLTGKKVVQLDVRGNMAKLNDGSQITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTLFRKIGDFRTLEKISREVKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILPEYLSNWTMEKVRREGVKVLPNAIVQSVGVSGGKLLIKLKDGRKVETDHIVAAVGLEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEAPEITLPVGSPAVPQAPTQGEDYGKGVIFYLRDKVVVGIVLWNVFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED 9E27DA0BDF8B9C61 aifm1_chlae Apoptosis-inducing factor 1, mitochondrial protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey similar to O95831 bag6_pig Large proline-rich protein BAG6 BCL2-associated athanogene 6 HLA-B-associated transcript 3 BAG6 BAT3 protein protein pig Sus scrofa (Pig) Pig MEPNDSTSTTMEEPESLEVLVKTLDSQTRTFIVGAQMNVKEFKEHIAASVSIPSEKQRLIYQGRVLQDDKKLQEYNVGGKVIHLVERAPPQTQLPSGASSGTGSASATHGGGPPPGTRGPGASVHDRNANSYVMVGTFNLPSDGSAVDVHINMEQAPIQSEPRVRLVMAQHMIRDIQTLLSRMECRGGSQAQHSQPPSQMPTVAPEPVALSSQTSESVESEVPPREPMAAEEVEERASAQSPGLSPSGPAPAGPTPAPETNAPNHPSPAEYVEVLQELQRLESRLQPFLQRYYEVLGAAATTDYNNNQEGREEDQRLINLVGESLRLLGNTFVALSDLRCNLACAPPRHLHVVRPMSHYTTPMVLQQAAIPIQINVGTTVTMTGNGTRPPPTPNAEAPPPGPGQASSLAPSSTTVESSTEGAPPPGPAPPPAASHPRVIRISHQSVEPVVMMHMNIQDSGTQPGGVPSAPTGPLGPTGHGQTLGQQVPGFPTAPTRVVIARPTPPQSRPSHPGGPPVSGALPGAGLGTNASLAQMVSGLVGQLLMQPVLVAQGTPGMAPPPAPATASASAGTTNTATTAGPAPGGPAQPPPPQPSASDLQFSQLLGNLLGPAGPGAGGPGMTSPTITVAMPGVPAFLQGMTDFLQATQTAAPPAPPPPPPPPPPAPEQQTAPPPGSPPGGAGSPGGLGPESLPLEFFTSVVQGVLSSLLGSLGARAGSSESIAAFIQRLSGSSNIFEPGADGALGFFGALLSLLCQNFSMVDVVMLLHGHFQPLQRLQPQLRSFFHQHYLGGQEPTPGNIRTATHTLITGLEEYVRESFSLVQVQPGVDIIRTNLEFLQEQFNSIAAHVMHCTDSGFGARLLELCNQGLFECLALNLHCLGGQQMELAAVINGRIRRMSRGVNPSLVSWLTTMMGLRLQVVLEHMPVGPDAILRYVRRVGDPPQPLPEEPMEVQGSERTSPEPQRENASPAPGTTAEEAMSRGPPPAPEGGSRDEQDGAAAETEPWAAAVPPEWVPIIQQDIQSQRKVKPQPPLSDAYLSGMPAKRRKTMQGEGPQLLLSEAVSRAAKAAGARPLTSPESLSRDLEAPEVQESYRQQLRADIQKRLQEDPNYSPQRFPNAHRAFAEDP AE1E659921C292B8 q04561-pro_0000036687 Nsp2 cysteine proteinase ORF1ab polyprotein 1a-1b rep protein protein PRRSV Porcine reproductive and respiratory syndrome virus (strain Lelystad) AAGKRARAKRAAKSEKDSAPTPKVALPVPTCGITTYSPPTDGSCGWHVLAAIMNRMINGDFTSPLTQYNRPEDDWASDYDLVQAIQCLRLPATVVRNRACPNAKYLIKLNGVHWEVEVRSGMAPRSLSRECVVGVCSEGCVAPPYPADGLPKRALEALASAYRLPSDCVSSGIADFLANPPPQEFWTLDKMLTSPSPERSGFSSLYKLLLEVVPQKCGATEGAFIYAVERMLKDCPSSKQAMALLAKIKVPSSKAPSVSLDECFPTDVLADFEPASQERPQSSGAAVVLCSPDAKEFEEAAPEEVQESGHKAVHSALLAEGPNNEQVQVVAGEQLKLGGCGLAVGNAHEGALVSAGLINLVGGNLSPSDPMKENMLNSREDEPLDLSQPAPASTTTLVREQTPDNPGSDAGALPVTVREFVPTGPILCHVEHCGTESGDSSSPLDLSDAQTLDQPLNLSLAAWPVRATASDPGWVHGRREPVFVKPRNAFSDGDSALQFGELSESSSVIEFDRTKDAPVVDAPVDLTTSNEALSVVDPFEFAELKRPRFSAQALIDRGGPLADVHAKIKNRVYEQCLQACEPGSRATPATREWLDKMWDRVDMKTWRCTSQFQAGRILASLKFLPDMIQDTPPPVPRKNRASDNAGLKQLVAQWDRKLSVTPPPKPVGPVLDQIVPPPTDIQQEDVTPSDGPPHAPDFPSRVSTGGSWKGLMLSGTRLAGSISQRLMTWVFEVFSHLPAFMLTLFSPRGSMAPGDWLFAGVVLLALLLCRSYPILGCLPLLGVFSGSLRRVRLGVFGSWMAFAVFLFSTPSNPVGSSCDHDSPECHAELLALEQRQLWEPVRGLVVGPSGLLCVILGKLLGGSRYLWHVLLRLCMLADLALSLVYVVSQGRCHKCWGKCIRTAPAEVALNVFPFSRATRVSLVSLCDRFQTPKGVDPVHLATGWRGCWRGESPIHQPHQKPIAYANLDEKKMSAQTVVAVPYDPSQAIKCLKVLQAGGAIVDQPTPEVVRVSEIPFSAPFFPKVPVNPDCRVVVDSDTFVAAVRCGYSTAQLVLGRGNFAKLNQTPPRNSISTKTTGG 386 1463 3B749F658CC2A97E nsp2-bag6-1 1 5 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 7A bag6-rep-1 1 5 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 7C aifm1-rep-1 1 3 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 7D rep-aifm1-1 1 3 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 7B nsp2-bag6-aif1-1 2 4 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 7E