UniProt UniProt http://www.uniprot.org Mutual antagonism between the Ebola virus VP35 protein and the RIG-I activator PACT determines infection outcome. Mutual antagonism between the Ebola virus VP35 protein and the RIG-I activator PACT determines infection outcome. Cell Host Microbe (1931-3128) 2013 imex curation Luthra P., Ramanan P., Mire C.E., Weisend C., Tsuda Y., Yen B., Liu G., Leung D.W., Geisbert T.W., Ebihara H., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins Origin of EBOV VP35 (reference strain) according pmid 11027311 (following Prins et al., 2010a); EBOV L and NP according pmid 23382680 (Shabman 2013). chris.basler@mssm.edu 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Luthra P., Ramanan P., Mire C.E., Weisend C., Tsuda Y., Yen B., Liu G., Leung D.W., Geisbert T.W., Ebihara H., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins Origin of EBOV VP35 (reference strain) according pmid 11027311 (following Prins et al., 2010a); EBOV L and NP according pmid 23382680 (Shabman 2013). chris.basler@mssm.edu Accepted 2014-OCT-07 by MGT Cell Host Microbe (1931-3128) 2013 Only protein-protein interactions imex curation Mutual antagonism between the Ebola virus VP35 protein and the RIG-I activator PACT determines infection outcome. Mutual antagonism between the Ebola virus VP35 protein and the RIG-I activator PACT determines infection outcome. Cell Host Microbe (1931-3128) 2013 imex curation Luthra P., Ramanan P., Mire C.E., Weisend C., Tsuda Y., Yen B., Liu G., Leung D.W., Geisbert T.W., Ebihara H., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins Origin of EBOV VP35 (reference strain) according pmid 11027311 (following Prins et al., 2010a); EBOV L and NP according pmid 23382680 (Shabman 2013). chris.basler@mssm.edu 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Luthra P., Ramanan P., Mire C.E., Weisend C., Tsuda Y., Yen B., Liu G., Leung D.W., Geisbert T.W., Ebihara H., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins Origin of EBOV VP35 (reference strain) according pmid 11027311 (following Prins et al., 2010a); EBOV L and NP according pmid 23382680 (Shabman 2013). chris.basler@mssm.edu Accepted 2014-OCT-07 by MGT Cell Host Microbe (1931-3128) 2013 Only protein-protein interactions imex curation Mutual antagonism between the Ebola virus VP35 protein and the RIG-I activator PACT determines infection outcome. Mutual antagonism between the Ebola virus VP35 protein and the RIG-I activator PACT determines infection outcome. Cell Host Microbe (1931-3128) 2013 imex curation Luthra P., Ramanan P., Mire C.E., Weisend C., Tsuda Y., Yen B., Liu G., Leung D.W., Geisbert T.W., Ebihara H., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins Origin of EBOV VP35 (reference strain) according pmid 11027311 (following Prins et al., 2010a); EBOV L and NP according pmid 23382680 (Shabman 2013). chris.basler@mssm.edu 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Luthra P., Ramanan P., Mire C.E., Weisend C., Tsuda Y., Yen B., Liu G., Leung D.W., Geisbert T.W., Ebihara H., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins Origin of EBOV VP35 (reference strain) according pmid 11027311 (following Prins et al., 2010a); EBOV L and NP according pmid 23382680 (Shabman 2013). chris.basler@mssm.edu Accepted 2014-OCT-07 by MGT Cell Host Microbe (1931-3128) 2013 Only protein-protein interactions imex curation Mutual antagonism between the Ebola virus VP35 protein and the RIG-I activator PACT determines infection outcome. Mutual antagonism between the Ebola virus VP35 protein and the RIG-I activator PACT determines infection outcome. Cell Host Microbe (1931-3128) 2013 imex curation Luthra P., Ramanan P., Mire C.E., Weisend C., Tsuda Y., Yen B., Liu G., Leung D.W., Geisbert T.W., Ebihara H., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins Origin of EBOV VP35 (reference strain) according pmid 11027311 (following Prins et al., 2010a); EBOV L and NP according pmid 23382680 (Shabman 2013). chris.basler@mssm.edu 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Luthra P., Ramanan P., Mire C.E., Weisend C., Tsuda Y., Yen B., Liu G., Leung D.W., Geisbert T.W., Ebihara H., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins Origin of EBOV VP35 (reference strain) according pmid 11027311 (following Prins et al., 2010a); EBOV L and NP according pmid 23382680 (Shabman 2013). chris.basler@mssm.edu Accepted 2014-OCT-07 by MGT Cell Host Microbe (1931-3128) 2013 Only protein-protein interactions imex curation ncap_ebozm Nucleoprotein NP Nucleocapsid protein Ebola NP protein protein ebozm Zaire ebolavirus (strain Mayinga-76) ZEBOV Zaire Ebola virus MDSRPQKIWMAPSLTESDMDYHKILTAGLSVQQGIVRQRVIPVYQVNNLEEICQLIIQAFEAGVDFQESADSFLLMLCLHHAYQGDYKLFLESGAVKYLEGHGFRFEVKKRDGVKRLEELLPAVSSGKNIKRTLAAMPEEETTEANAGQFLSFASLFLPKLVVGEKACLEKVQRQIQVHAEQGLIQYPTAWQSVGHMMVIFRLMRTNFLIKFLLIHQGMHMVAGHDANDAVISNSVAQARFSGLLIVKTVLDHILQKTERGVRLHPLARTAKVKNEVNSFKAALSSLAKHGEYAPFARLLNLSGVNNLEHGLFPQLSAIALGVATAHGSTLAGVNVGEQYQQLREAATEAEKQLQQYAESRELDHLGLDDQEKKILMNFHQKKNEISFQQTNAMVTLRKERLAKLTEAITAASLPKTSGHYDDDDDIPFPGPINDDDNPGHQDDDPTDSQDTTIPDVVVDPDDGSYGEYQSYSENGMNAPDDLVLFDLDEDDEDTKPVPNRSTKGGQQKNSQKGQHIEGRQTQSRPIQNVPGPHRTIHHASAPLTDNDRRNEPSGSTSPRMLTPINEEADPLDDADDETSSLPPLESDDEEQDRDGTSNRTPTVAPPAPVYRDHSEKKELPQDEQQDQDHTQEARNQDSDNTQSEHSFEEMYRHILRSQGPFDAVLYYHMMKDEPVVFSTSDGKEYTYPDSLEEEYPPWLTEKEAMNEENRFVTLDGQQFYWPVMNHKNKFMAILQHHQ 159C254EA0478886 ddx58_human Antiviral innate immune response receptor RIG-I DEAD box protein 58 Retinoic acid-inducible gene 1 protein DDX58 Probable ATP-dependent RNA helicase DDX58 Retinoic acid-inducible gene I protein RIG-I-like receptor 1 protein protein human Homo sapiens Human MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK BF0D501C395BAE25 vp35_ebozm Polymerase cofactor VP35 VP35 Ebola VP35 protein protein ebozm Zaire ebolavirus (strain Mayinga-76) ZEBOV Zaire Ebola virus MTTRTKGRGHTAATTQNDRMPGPELSGWISEQLMTGRIPVSDIFCDIENNPGLCYASQMQQTKPNPKTRNSQTQTDPICNHSFEEVVQTLASLATVVQQQTIASESLEQRITSLENGLKPVYDMAKTISSLNRVCAEMVAKYDLLVMTTGRATATAAATEAYWAEHGQPPPGPSLYEESAIRGKIESRDETVPQSVREAFNNLNSTTSLTEENFGKPDISAKDLRNIMYDHLPGFGTAFHQLVQVICKLGKDSNSLDIIHAEFQASLAEGDSPQCALIQITKRVPIFQDAAPPVIHIRSRGDIPRACQKSLRPVPPSPKIDRGWVCVFQLQDGKTLGLKI DIP protein Q05127 original sequence version: 43 B171C7FDBB5B1FBD l_ebozm RNA-directed RNA polymerase L L Large structural protein Replicase Transcriptase protein protein ebozm Zaire ebolavirus (strain Mayinga-76) ZEBOV Zaire Ebola virus MATQHTQYPDARLSSPIVLDQCDLVTRACGLYSSYSLNPQLRNCKLPKHIYRLKYDVTVTKFLSDVPVATLPIDFIVPVLLKALSGNGFCPVEPRCQQFLDEIIKYTMQDALFLKYYLKNVGAQEDCVDEHFQEKILSSIQGNEFLHQMFFWYDLAILTRRGRLNRGNSRSTWFVHDDLIDILGYGDYVFWKIPISMLPLNTQGIPHAAMDWYQASVFKEAVQGHTHIVSVSTADVLIMCKDLITCRFNTTLISKIAEIEDPVCSDYPNFKIVSMLYQSGDYLLSILGSDGYKIIKFLEPLCLAKIQLCSKYTERKGRFLTQMHLAVNHTLEEITEMRALKPSQAQKIREFHRTLIRLEMTPQQLCELFSIQKHWGHPVLHSETAIQKVKKHATVLKALRPIVIFETYCVFKYSIAKHYFDSQGSWYSVTSDRNLTPGLNSYIKRNQFPPLPMIKELLWEFYHLDHPPLFSTKIISDLSIFIKDRATAVERTCWDAVFEPNVLGYNPPHKFSTKRVPEQFLEQENFSIENVLSYAQKLEYLLPQYRNFSFSLKEKELNVGRTFGKLPYPTRNVQTLCEALLADGLAKAFPSNMMVVTEREQKESLLHQASWHHTSDDFGEHATVRGSSFVTDLEKYNLAFRYEFTAPFIEYCNRCYGVKNVFNWMHYTIPQCYMHVSDYYNPPHNLTLENRDNPPEGPSSYRGHMGGIEGLQQKLWTSISCAQISLVEIKTGFKLRSAVMGDNQCITVLSVFPLETDADEQEQSAEDNAARVAASLAKVTSACGIFLKPDETFVHSGFIYFGKKQYLNGVQLPQSLKTATRMAPLSDAIFDDLQGTLASIGTAFERSISETRHIFPCRITAAFHTFFSVRILQYHHLGFNKGFDLGQLTLGKPLDFGTISLALAVPQVLGGLSFLNPEKCFYRNLGDPVTSGLFQLKTYLRMIEMDDLFLPLIAKNPGNCTAIDFVLNPSGLNVPGSQDLTSFLRQIVRRTITLSAKNKLINTLFHASADFEDEMVCKWLLSSTPVMSRFAADIFSRTPSGKRLQILGYLEGTRTLLASKIINNNTETPVLDRLRKITLQRWSLWFSYLDHCDNILAEALTQITCTVDLAQILREYSWAHILEGRPLIGATLPCMIEQFKVFWLKPYEQCPQCSNAKQPGGKPFVSVAVKKHIVSAWPNASRISWTIGDGIPYIGSRTEDKIGQPAIKPKCPSAALREAIELASRLTWVTQGSSNSDLLIKPFLEARVNLSVQEILQMTPSHYSGNIVHRYNDQYSPHSFMANRMSNSATRLIVSTNTLGEFSGGGQSARDSNIIFQNVINYAVALFDIKFRNTEATDIQYNRAHLHLTKCCTREVPAQYLTYTSTLDLDLTRYRENELIYDSNPLKGGLNCNISFDNPFFQGKRLNIIEDDLIRLPHLSGWELAKTIMQSIISDSNNSSTDPISSGETRSFTTHFLTYPKIGLLYSFGAFVSYYLGNTILRTKKLTLDNFLYYLTTQIHNLPHRSLRILKPTFKHASVMSRLMSIDPHFSIYIGGAAGDRGLSDAARLFLRTSISSFLTFVKEWIINRGTIVPLWIVYPLEGQNPTPVNNFLYQIVELLVHDSSRQQAFKTTISDHVHPHDNLVYTCKSTASNFFHASLAYWRSRHRNSNRKYLARDSSTGSSTNNSDGHIERSQEQTTRDPHDGTERNLVLQMSHEIKRTTIPQENTHQGPSFQSFLSDSACGTANPKLNFDRSRHNVKFQDHNSASKREGHQIISHRLVLPFFTLSQGTRQLTSSNESQTQDEISKYLRQLRSVIDTTVYCRFTGIVSSMHYKLDEVLWEIESFKSAVTLAEGEGAGALLLIQKYQVKTLFFNTLATESSIESEIVSGMTTPRMLLPVMSKFHNDQIEIILNNSASQITDITNPTWFKDQRARLPKQVEVITMDAETTENINRSKLYEAVYKLILHHIDPSVLKAVVLKVFLSDTEGMLWLNDNLAPFFATGYLIKPITSSARSSEWYLCLTNFLSTTRKMPHQNHLSCKQVILTALQLQIQRSPYWLSHLTQYADCELHLSYIRLGFPSLEKVLYHRYNLVDSKRGPLVSITQHLAHLRAEIRELTNDYNQQRQSRTQTYHFIRTAKGRITKLVNDYLKFFLIVQALKHNGTWQAEFKKLPELISVCNRFYHIRDCNCEERFLVQTLYLHRMQDSEVKLIERLTGLLSLFPDGLYRFD 5B07EDDC370E2934 prkra_human Interferon-inducible double-stranded RNA-dependent protein kinase activator A Protein activator of the interferon-induced protein kinase PKR-associated protein X PKR-associating protein X HSD-14 PRKRA RAX PACT HSD14 Protein kinase, interferon-inducible double-stranded RNA-dependent activator protein protein human Homo sapiens Human MSQSRHRAEAPPLEREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGCTWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAELSTSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK 9B01637E6194827E vp35-prkra 4 9 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection Q05127:p.Arg312Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:14:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg312ala' Tue Jun 30 18:07:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg312Ala' Q05127:p.[Lys319Ala;Arg322Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 14:43:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys319ala,arg322ala' Tue Jun 30 20:26:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys319Ala;Arg322Ala]' Q05127:p.Phe239Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain F A Wed May 02 17:03:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe239ala' Tue Jun 30 16:51:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe239Ala' physical association physical association 3A and D ddx58-prkra 1 6 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 3B and E Association is disrupted by coexpression of EBOV wildtype VP35 (UniProt AC O95786). Expression of EBOV VP35 mutants unable to associate with human HA-tagged PRKRA/PACT (R312A, F239A and K319A/R322A) has no effect. prkra-ddx58 3 6 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection 9 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection physical association physical association 3C Association detected in Sendai virus and mock infected cells. Association is disrupted by stable expression of EBOV VP35 (UniProt AC Q05127) vp35-np 2 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection physical association physical association 5C Coexpression of HA-tagged PRKRA/PACT does not change the association. vp35-l 4 8 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection physical association physical association 5D and S3 Coexpression of HA-tagged wildtype PRKRA/PACT decreases the interaction;this effect is not seen when using mutant EBOV VP35 K319A/R322A which is unable to associate with PRKRA/PACT. vp35-prkra-1 1 9 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range ifn_inhibitory_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association S1A