HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation. Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation. PLoS ONE 2013 imex curation Yim S.A., Lim Y.S., Kim J.W., Hwang S.B. Virus - Publications including interactions involving viral proteins sbhwang@hallym.ac.kr 14-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yim S.A., Lim Y.S., Kim J.W., Hwang S.B. Virus - Publications including interactions involving viral proteins sbhwang@hallym.ac.kr Accepted 2014-APR-02 by ORCHARD PLoS ONE 2013 imex curation Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation. Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation. PLoS ONE 2013 imex curation Yim S.A., Lim Y.S., Kim J.W., Hwang S.B. Virus - Publications including interactions involving viral proteins sbhwang@hallym.ac.kr 14-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells confocal microscopy confocal microscopy immunostaining immunostaining Yim S.A., Lim Y.S., Kim J.W., Hwang S.B. Virus - Publications including interactions involving viral proteins sbhwang@hallym.ac.kr Accepted 2014-APR-02 by ORCHARD PLoS ONE 2013 imex curation Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation. Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation. PLoS ONE 2013 imex curation Yim S.A., Lim Y.S., Kim J.W., Hwang S.B. Virus - Publications including interactions involving viral proteins sbhwang@hallym.ac.kr 14-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells cosedimentation cosedimentation western blot western blot Immuno blot Immuno blot Yim S.A., Lim Y.S., Kim J.W., Hwang S.B. Virus - Publications including interactions involving viral proteins sbhwang@hallym.ac.kr Accepted 2014-APR-02 by ORCHARD PLoS ONE 2013 imex curation Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation. Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation. PLoS ONE 2013 imex curation Yim S.A., Lim Y.S., Kim J.W., Hwang S.B. Virus - Publications including interactions involving viral proteins sbhwang@hallym.ac.kr 14-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells tap tandem affinity purification TAP TAP tap de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Yim S.A., Lim Y.S., Kim J.W., Hwang S.B. Virus - Publications including interactions involving viral proteins sbhwang@hallym.ac.kr Accepted 2014-APR-02 by ORCHARD PLoS ONE 2013 imex curation Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation. Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation. PLoS ONE 2013 imex curation Yim S.A., Lim Y.S., Kim J.W., Hwang S.B. Virus - Publications including interactions involving viral proteins sbhwang@hallym.ac.kr 14-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yim S.A., Lim Y.S., Kim J.W., Hwang S.B. Virus - Publications including interactions involving viral proteins sbhwang@hallym.ac.kr Accepted 2014-APR-02 by ORCHARD PLoS ONE 2013 imex curation Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation. Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation. PLoS ONE 2013 imex curation Yim S.A., Lim Y.S., Kim J.W., Hwang S.B. Virus - Publications including interactions involving viral proteins sbhwang@hallym.ac.kr 14-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yim S.A., Lim Y.S., Kim J.W., Hwang S.B. Virus - Publications including interactions involving viral proteins sbhwang@hallym.ac.kr Accepted 2014-APR-02 by ORCHARD PLoS ONE 2013 imex curation q03463-pro_0000278740 Non-structural protein 5A protein protein hcvj1 Hepatitis C virus genotype 1b (isolate HC-J1) (HCV) SGSWLRDIWDWICEVLSDFKTWLKTKLMPHLPGIPFVSCQHGYKGVWRGDGIMHTRCHCGAEITGHVKNGTMRIVGPKTCRNMWSGTFPINAYTTGPCTPLPAPNYTFALWRVSAEEYVEIRRVGDFHYVTGMTTDNLKCPCQVPSPEFFTELDGVRLHRFAPPCKPLLREEVSFRVGLHDYPVGSQLPCEPEPDVAVLTSMLTDPSHITAAAAGRRLARGSPPSEASSSASQLSAPSLKATCTINHDSPDAELIEANLLWRQEMGGNITRVESENKVVILDSFDPLVAEEDEREISVPAEILRKSRRFTQALPIWARPDYNPPLIETWKKPNYEPPVVHGCPLPPPQSPPVPPPRKKRTVVLTESTLSTALAELAAKSFGSSSTSGITGDNTTTSSEPAPSGCSPDSDAESYSSMPPLEGEPGDPDLSDGSWSTVSSEAGTEDVVCC 1973 2420 587F253C2E2AE2BC pdia1_human Protein disulfide-isomerase P4HB PDI PDIA1 PO4DB Prolyl 4-hydroxylase subunit beta Cellular thyroid hormone-binding protein p55 ERBA2L protein protein human Homo sapiens Human MLRRALLCLAVAALVRADAPEEEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGRNNFEGEVTKENLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL DIP protein P07237 original sequence version: 198 906CE6D9900B8FCE q99ib8-pro_0000045602 Non-structural protein 5A protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV SGSWLRDVWDWVCTILTDFKNWLTSKLFPKLPGLPFISCQKGYKGVWAGTGIMTTRCPCGANISGNVRLGSMRITGPKTCMNTWQGTFPINCYTEGQCAPKPPTNYKTAIWRVAASEYAEVTQHGSYSYVTGLTTDNLKIPCQLPSPEFFSWVDGVQIHRFAPTPKPFFRDEVSFCVGLNSYAVGSQLPCEPEPDADVLRSMLTDPPHITAETAARRLARGSPPSEASSSVSQLSAPSLRATCTTHSNTYDVDMVDANLLMEGGVAQTEPESRVPVLDFLEPMAEEESDLEPSIPSECMLPRSGFPRALPAWARPDYNPPLVESWRRPDYQPPTVAGCALPPPKKAPTPPPRRRRTVGLSESTISEALQQLAIKTFGQPPSSGDAGSSTGAGAAESGGPTSPGEPAPSETGSASSMPPLEGEPGDPDLESDQVELQPPPQGGGVAPGSGSGSWSTCSEEDDTTVCC 1977 2442 0BDEA498491643D8 pyc_human Pyruvate carboxylase, mitochondrial PC Pyruvic carboxylase protein protein human Homo sapiens Human MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE DIP protein P11498 original sequence version: 108 381F527553A20095 ns5a-pdi-pc-1 4 7 unspecified role unspecified role bait bait tap tag tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey association association Fig. 1A Actin was also identified in this complex but could not be mapped to a single gene product. pc-ns5a-4 6 10 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Fig. 1C pc-ns5a-1 5 10 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2A pc-ns5a-2 1 10 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 30 27 physical association physical association Fig. 2B, C and D pc-ns5a-5 2 7 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 2E ns5a-pc-1 5 10 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2A ns5a-pc-2 1 9 unspecified role unspecified role bait bait myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 2B pc-ns5a-3 3 7 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 2 F VDAC proteins were also present but could not be mapped to a single gene product