HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Hepatitis C virus NS4B blocks the interaction of STING and TBK1 to evade host innate immunity. Hepatitis C virus NS4B blocks the interaction of STING and TBK1 to evade host innate immunity. J. Hepatol. (0168-8278) 2013 imex curation Ding Q., Cao X., Lu J., Huang B., Liu Y.J., Kato N., Shu H.B., Zhong J. Virus - Publications including interactions involving viral proteins jzhong@sibs.ac.cn 28-Oct_2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293a Homo sapiens hek293a hek293a anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ding Q., Cao X., Lu J., Huang B., Liu Y.J., Kato N., Shu H.B., Zhong J. Virus - Publications including interactions involving viral proteins Origin of MAVS inferred from sequnce length and mutant mapping. Origin of TBK1 inferred from work of donating author (PMID: 23585680) jzhong@sibs.ac.cn Accepted 2013-OCT-21 by ORCHARD 28-Oct_2013: Contacted by IntAct-Help. J. Hepatol. (0168-8278) 2013 imex curation Hepatitis C virus NS4B blocks the interaction of STING and TBK1 to evade host innate immunity. Hepatitis C virus NS4B blocks the interaction of STING and TBK1 to evade host innate immunity. J. Hepatol. (0168-8278) 2013 imex curation Ding Q., Cao X., Lu J., Huang B., Liu Y.J., Kato N., Shu H.B., Zhong J. Virus - Publications including interactions involving viral proteins jzhong@sibs.ac.cn 28-Oct_2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-ph5ch8 Human primary hepatocyte ph5ch8 ph5ch8 Primary human hepatocyte cell line PH5CH8 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ding Q., Cao X., Lu J., Huang B., Liu Y.J., Kato N., Shu H.B., Zhong J. Virus - Publications including interactions involving viral proteins jzhong@sibs.ac.cn Accepted 2013-OCT-21 by ORCHARD 28-Oct_2013: Contacted by IntAct-Help. J. Hepatol. (0168-8278) 2013 imex curation Hepatitis C virus NS4B blocks the interaction of STING and TBK1 to evade host innate immunity. Hepatitis C virus NS4B blocks the interaction of STING and TBK1 to evade host innate immunity. J. Hepatol. (0168-8278) 2013 imex curation Ding Q., Cao X., Lu J., Huang B., Liu Y.J., Kato N., Shu H.B., Zhong J. Virus - Publications including interactions involving viral proteins jzhong@sibs.ac.cn 28-Oct_2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-ph5ch8 Human primary hepatocyte ph5ch8 ph5ch8 Primary human hepatocyte cell line PH5CH8 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ding Q., Cao X., Lu J., Huang B., Liu Y.J., Kato N., Shu H.B., Zhong J. Virus - Publications including interactions involving viral proteins jzhong@sibs.ac.cn Accepted 2013-OCT-21 by ORCHARD 28-Oct_2013: Contacted by IntAct-Help. J. Hepatol. (0168-8278) 2013 imex curation Hepatitis C virus NS4B blocks the interaction of STING and TBK1 to evade host innate immunity. Hepatitis C virus NS4B blocks the interaction of STING and TBK1 to evade host innate immunity. J. Hepatol. (0168-8278) 2013 imex curation Ding Q., Cao X., Lu J., Huang B., Liu Y.J., Kato N., Shu H.B., Zhong J. Virus - Publications including interactions involving viral proteins jzhong@sibs.ac.cn 28-Oct_2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Ding Q., Cao X., Lu J., Huang B., Liu Y.J., Kato N., Shu H.B., Zhong J. Virus - Publications including interactions involving viral proteins jzhong@sibs.ac.cn Accepted 2013-OCT-21 by ORCHARD 28-Oct_2013: Contacted by IntAct-Help. J. Hepatol. (0168-8278) 2013 imex curation Hepatitis C virus NS4B blocks the interaction of STING and TBK1 to evade host innate immunity. Hepatitis C virus NS4B blocks the interaction of STING and TBK1 to evade host innate immunity. J. Hepatol. (0168-8278) 2013 imex curation Ding Q., Cao X., Lu J., Huang B., Liu Y.J., Kato N., Shu H.B., Zhong J. Virus - Publications including interactions involving viral proteins jzhong@sibs.ac.cn 28-Oct_2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-ph5ch8 Human primary hepatocyte ph5ch8 ph5ch8 Primary human hepatocyte cell line PH5CH8 confocal microscopy confocal microscopy immunostaining immunostaining Ding Q., Cao X., Lu J., Huang B., Liu Y.J., Kato N., Shu H.B., Zhong J. Virus - Publications including interactions involving viral proteins jzhong@sibs.ac.cn Accepted 2013-OCT-21 by ORCHARD 28-Oct_2013: Contacted by IntAct-Help. J. Hepatol. (0168-8278) 2013 imex curation sting_human Stimulator of interferon genes protein STING1 ERIS Endoplasmic reticulum interferon stimulator MITA Mediator of IRF3 activation TMEM173 Transmembrane protein 173 protein protein human Homo sapiens Human MPHSSLHPSIPCPRGHGAQKAALVLLSACLVTLWGLGEPPEHTLRYLVLHLASLQLGLLLNGVCSLAEELRHIHSRYRGSYWRTVRACLGCPLRRGALLLLSIYFYYSLPNAVGPPFTWMLALLGLSQALNILLGLKGLAPAEISAVCEKGNFNVAHGLAWSYYIGYLRLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTGDHAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSREDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEVTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS DIP protein Q86WV6 original sequence version: 83 DIP protein Q86WV6 original sequence version: 47 DIP protein Q86WV6 original sequence version: 50 DIP protein Q86WV6 original sequence version: 100 DIP protein Q86WV6 original sequence version: 68 CB54D6A4D4D8E7C0 q99ib8-pro_0000045601 Non-structural protein 4B protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV ASRAALIEEGQRIAEMLKSKIQGLLQQASKQAQDIQPAMQASWPKVEQFWARHMWNFISGIQYLAGLSTLPGNPAVASMMAFSAALTSPLSTSTTILLNIMGGWLASQIAPPAGATGFVVSGLVGAAVGSIGLGKVLVDILAGYGAGISGALVAFKIMSGEKPSMEDVINLLPGILSPGALVVGVICAAILRRHVGPGEGAVQWMNRLIAFASRGNHVAPTHYVTESDASQRVTQLLGSLTITSLLRRLHNWITEDCPIPC 1716 1976 F4790FECE9E4F8C0 tbk1_human Serine/threonine-protein kinase TBK1 TBK1 NAK TANK-binding kinase 1 T2K NF-kappa-B-activating kinase protein protein human Homo sapiens Human MQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKVFNNISFLRPVDVQMREFEVLKKLNHKNIVKLFAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDVVGGMNHLRENGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQFVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSLPFRPFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPVLANILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQMTAHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAITGVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLAYNEEQIHKFDKQKLYYHATKAMTHFTDECVKKYEAFLNKSEEWIRKMLHLRKQLLSLTNQCFDIEEEVSKYQEYTNELQETLPQKMFTASSGIKHTMTPIYPSSNTLVEMTLGMKKLKEEMEGVVKELAENNHILERFGSLTMDGGLRNVDCL DIP protein Q9UHD2 original sequence version: 116 DIP protein Q9UHD2 original sequence version: 120 DIP protein Q9UHD2 original sequence version: 79 DIP protein Q9UHD2 original sequence version: null B58E4FE1B502276D mavs_human Mitochondrial antiviral-signaling protein Interferon beta promoter stimulator protein 1 Virus-induced-signaling adapter CARD adapter inducing interferon beta Putative NF-kappa-B-activating protein 031N MAVS IPS1 KIAA1271 VISA protein protein human Homo sapiens Human MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYQSYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEQDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPSKLPINSTRAGMVPSKVPTSMVLTKVSASTVPTDGSSRNEETPAAPTPAGATGGSSAWLDSSSENRGLGSELSKPGVLASQVDSPFSGCFEDLAISASTSLGMGPCHGPEENEYKSEGTFGIHVAENPSIQLLEGNPGPPADPDGGPRPQADRKFQEREVPCHRPSPGALWLQVAVTGVLVVTLLVVLYRRRLH 0E23E3E115941EE8 ns4b-sting-5 1 STING 6 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NS4B 7 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2A mavs-sting-1 1 MAVS 9 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined STING 6 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. fig. 2 tbk1-sting-1 1 TBK1 8 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined STING 6 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. fig. 2 NS4B inhibit TBK1-STING interaction ns4b-sting-6 3 STING 6 unspecified role unspecified role prey prey NS4B 7 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2C ns4b-sting-2 5 STING 6 unspecified role unspecified role neutral component neutral component NS4B 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 2D ns4b-sting-3 4 STING 6 unspecified role unspecified role neutral component neutral component myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NS4B 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 2E string-string-2 1 STING 6 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined STING 6 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. fig. 2B mavs-sting-2 2 MAVS 9 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined STING 6 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3C ns4b-sting-7 2 STING 6 unspecified role unspecified role prey prey NS4B 7 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2C tbk1-sting-2 2 TBK1 8 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined STING 6 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3D NS4B inhibit TBK1-STING interaction string-string-1 2 STING 6 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined STING 6 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3B