HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html The herpesvirus VP1/2 protein is an effector of dynein-mediated capsid transport and neuroinvasion. The herpesvirus VP1/2 protein is an effector of dynein-mediated capsid transport and neuroinvasion. Cell host & microbe 2013 imex curation Zaichick SV., Bohannon KP., Hughes A., Sollars PJ., Pickard GE., Smith GA. Virus - Publications including interactions involving viral proteins g-smith3@northwestern.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zaichick SV., Bohannon KP., Hughes A., Sollars PJ., Pickard GE., Smith GA. Virus - Publications including interactions involving viral proteins VP1/2 also interact with Dynein but the specific Dynein intermediate chain could not be traced g-smith3@northwestern.edu Accepted 2016-JUN-13 AT 14:41 BST AT 14:41 BST by MGT Cell host & microbe 2013 imex curation The herpesvirus VP1/2 protein is an effector of dynein-mediated capsid transport and neuroinvasion. The herpesvirus VP1/2 protein is an effector of dynein-mediated capsid transport and neuroinvasion. Cell host & microbe 2013 imex curation Zaichick SV., Bohannon KP., Hughes A., Sollars PJ., Pickard GE., Smith GA. Virus - Publications including interactions involving viral proteins g-smith3@northwestern.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Zaichick SV., Bohannon KP., Hughes A., Sollars PJ., Pickard GE., Smith GA. Virus - Publications including interactions involving viral proteins g-smith3@northwestern.edu Accepted 2016-JUN-13 AT 14:41 BST AT 14:41 BST by MGT Cell host & microbe 2013 imex curation dctn2_human Dynactin subunit 2 Dynactin complex 50 kDa subunit 50 kDa dynein-associated polypeptide p50 dynamitin DCTN2 DCTN50 protein protein human Homo sapiens Human MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK DIP protein Q13561 original sequence version: 155 DIP protein Q13561 original sequence version: 160 C2FF01A9739337B2 dctn1_human Dynactin subunit 1 DCTN1 150 kDa dynein-associated polypeptide DAP-150 p150-glued p135 protein protein human Homo sapiens Human MAQSKRHVYSRTPSGSRMSAEASARPLRVGSRVEVIGKGHRGTVAYVGATLFATGKWVGVILDEAKGKNDGTVQGRKYFTCDEGHGIFVRQSQIQVFEDGADTTSPETPDSSASKVLKREGTDTTAKTSKLRGLKPKKAPTARKTTTRRPKPTRPASTGVAGASSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPVLTSPGAVPPLPSPSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRKQAQEKFELSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSAHERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPEDCTMQLADHIKFTQSALDCMSVEVGRLRAFLQGGQEATDIALLLRDLETSCSDIRQFCKKIRRRMPGTDAPGIPAALAFGPQVSDTLLDCRKHLTWVVAVLQEVAAAAAQLIAPLAENEGLLVAALEELAFKASEQIYGTPSSSPYECLRQSCNILISTMNKLATAMQEGEYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSAAKDADERIEKVQTRLEETQALLRKKEKEFEETMDALQADIDQLEAEKAELKQRLNSQSKRTIEGLRGPPPSGIATLVSGIAGEEQQRGAIPGQAPGSVPGPGLVKDSPLLLQQISAMRLHISQLQHENSILKGAQMKASLASLPPLHVAKLSHEGPGSELPAGALYRKTSQLLETLNQLSTHTHVVDITRTSPAAKSPSAQLMEQVAQLKSLSDTVEKLKDEVLKETVSQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGFGQRHRLVLTQEQLHQLHSRLIS DIP protein Q14203 original sequence version: null DIP protein Q14203 original sequence version: 143 DIP protein Q14203 original sequence version: 135 DIP protein Q14203 original sequence version: 140 DIP protein Q14203-1 original sequence version: 146 DIP protein Q14203-1 original sequence version: 112 6DCEA5E67856E4BC q83419_9alph UL25 protein protein pseudorabies virus pseudorabies virus MDRAWFAFEAAAIPGSARHFIAPPFPVGFWARPGFSEGLDARLALAHANARRRAAAAALDNAMAAGARLEAEVDEQLRPLERQVERVAEALVVLEETARAAEEADAARAAEEAPEATAAADEGREVQIAKNDVALAYDANLSLDFLAMVYAARGAASGVLFGTWYATLQATLVAERPQVSRAIDSRDGRMSRTFMGVTTTALQACGRLYVGNRHYSALESAALCLHLVHRVRQGAGAAGAAAPLGIADLLERVPEYLDALSQALAEGGRISYRYNYPRVPREQLHGRYALEGHSVLAALARLRVVPGANVGANEVDGAGFVDEVNRAAAAFLGRGQNLFLGEDAPLLRATVNTITGLLLLRRLLHNGNVYGDRLRNNFQLGALVPNAPPPRGASGDAPASRSGDGNLRFLLAHYVVVAYRADERTELTQLFPGLAALALDAHSIRARVQRHQLNLVRLVALELQNRQRVTAPVNEVIAAHDAVAVQYEEGLGLLLQQPHLRNAADKRLGQFGVSSDYDLLYFLCLGYIPQFAAA 9BBA7F78AF9644E0 g3g960_9alph UL36 protein protein pseudorabies virus pseudorabies virus MTADAVVVGYRNQYDPDLGPGSGVSCLRSSLSFLRLVFTNGVEAALTADAVDACLREGQAWTGPGGGMCAISELPNKIVYEGAGGAALHCVFSRVHGECNFFTPPAEGFQSTQITARAFFDGLWAQRAESFTLVVVGAVGLGLYRRGDVVYVFDPHGHGQITQAFVARLRPADVYAHVAGYAASELRWAGAFVFFLPHDQFAAVPALYGVGETYLVDEPYVERAVPSVHPMALPPDPAAAAPPTRGPGGIPVLAVAPVPAVPVSAPGGAPLPPSPRVQKRPSMPKRRRPPWPASQEDLSSTHTVGGRPSRKFRPRTREPMGFAVRELELAPLDLGPLQAHADELEEHVVACAGRSLFPGRAGLLERFAELLFGHLQDFLVENGARTHGEAPCAVAALFEPILGALPERGPAGDFVAATHMVLTEARALLDVAGDDASAPVAKLALVAARVARPANEAMHRALDALESEGSYAQLSACFMDAAARDPGGVYDQTVVARARALFARARARESRESQRALERARDVSALEAALEAAHANFDAVDAGAPGAGGAAEPNTRDVKERAAKLPRKLALMVGDAIREYFLRGAQYSAKAMLMDRNGAQRFRVANAAVAGLRRLVDSLAAAEERLRALDAAPAAHDGALLAQLLEAGEDVSTDERLGAWFALLAEAQAHGHLERRELDTLTRDVTQINERAAKRASADADLARFDAMDAALAAAPDEETRVRAMEDLLRHIRAMGGHVLSQETRARLARRDAELRTLVQAAQQRAAEQNDLYMRLRALLGDFATLGPAVDLLAHITATPVPGTVLAALWRLVARYRELLTSDPGALVGAGQPLGALVNALADNPPITRFFLAHADAVAAALADADTAEKAARAAGVLRAAVEHPELGFLEPLARQCEERAAALRESERVAALQAALSAAVAAAVEMLGRLRAQPDEGTLVEARHLALEVARAAAVEEPQLQKMALAARQRVGELEAAVAAVEARRAAEVERAQRQLWARAADAALRRAEQHAFDAAEFVRLRDVAADRGYDAAELRPAAERALGANAETVTTALDAVFAFNPHLPENAGAAAGPSLQALQRLTWLDDYAVAAPVIGSLFGADTEPLSRLARIAGGVLARAAAGMGRFSYYDAVGFVGADMAHVPRLAKYVDFYRRGFPEYEAELAALGARRADVLQAGGSRGAEICRALEEVTYVRAAADAARALDEGVHLHVPSTELLRGAVTYLERFDFARFERTAYAEAMRALVRRDLAAARDGLAAAEADQAAATERATRLLREVVDAAAARDRDGALELANLKNLLRVTPPPASLNIDRAASAEELVTQAALLLGAVEEAPELDVQAVEWLHQARLIIDTHPLTTRVDGRGPMDEFAERIEALQTLRQEADTLRQRLTAAEVAWDEAWEDFGRARGRAGASEDEARLAEARARALQTATMEVLALRSDAVYPRLPAKFVGRIESKYSARAGALGDFFRDAGAVEEDVRELDRMAARIGPELRHDALRALAAAFDARAERLPRWIPPKYAAYRELLTLRLQFYAAYGGAGPTPARPRASTAPCARLKQRVAARLGDRDVICTVREARSDADAVFPTVFLDDNNVPVEYQVCYRAVGDKLAAMLCTDEGAELRPPLTEGLMETATVAGMRLMNEIIHLRLNYEKARDTGFATFARFVRHRRADWDSAAKAAAETYAAVLATTLSRTFGALDRVAHPPLEPAVRLALPDLMVAAVAGEPTHLFNFTRVDLAGQHEYMAKTLDAVLDRALDAVDTLDAGARPPRRPLRDGQALFTLRVADWTEGRFSETELWAPWAHTETGQRLARAVPGALATFTVLARMCIPPRLLSALWSALQPEANAAYDAAVTERLDAAAHVREAAALGAPAPGDLYAPVGTGMTFTVTGSPPSSATRVSAMDLAAAAVLVGAPLVIAMENLDVYSPGSGLTLAVTLCDLRDPALHRGVSTDLSSWGERLLALDDNPIENACLAPQLERLSALIAARPLAGEPACLVLVDSQLAVSRVLWAPPEPPRRPGFTLEDDPLLREIPFLETTEDQLPEMDPADPLFTSIIMGDARVDPATSEELYGRPSFRNLGDPFPYAVSATPTRPATPIPGLPPELAGEDDGFVPDDDFGANPWLDLLGDDLVASALPLPPSPPVDPPTLPPLAPAAPAAPAAPPPAKPAEPTPAAKLAPPAPPPAKPAEPTPAAKLAPPAPPPAKPVETTPLTKPQPQPQQGPPAAHKKPAAGTKAAAAPKQQPREPAPKPHSSPRKIQPSLKARIEPPPPVINPPYPATAPAPETAPPEAPQAQPPAAAKPTPQPQGPPPPPQPPSAQAPPAQKPPAQPAAAATAATATTAPKANPQTQPPTRAQTQTAPPPPSAATAAAQVPPQPPSSQPAAKPRGAPPAPPAPPPPSAQTTLPRPAAPPAPPPPSAPAATPTPPAPGPAPSAKKSDGDRIVEPKAGAPPDVRDAKFGGKVAGTASPGRPFDDERYKAGRADKCEHTVGPDQHRTRSPPGTSDSEPASASSSAASTPSREAASSSSSPAGAPSDGARGGPPSAAGAAARAPPGAPGSGGQRRGDGGPAADASRGDQPAGHLPAAPVAQPLPSAVLRTKARTSVGIRDHSRRKSMALVPRRPVSAPRQTGGLPPAAQPEGRPPGGLEPRPPQAQAQAPATAPTGPPAAAAAAAATAAVPAPGVGPGGPVRRAAGPRPPAGHLRLHIPPPQPVQLEGVVVPLPASPETPAPAQTQPPRSPLAAPTSLAAPSEIERPAASAVVAAATTTTTSSSSSATTSSAPAPPPSRPAPPPSRPAPPPSRPAPPPPAPPPSRPAPTPERHGEPPRLMFTPPPLPPQPSQQRPPEAPWTWPEPRDSAGPPALFTPPPSDPPLQFPGREEPPLPPPPPPQPLWASEGEAETPATPPRSPLERMPWPPPLEAPGTTPELPPEFAPPAPRSPLADPHQPPAWFDDLTALVPPAHPPPRRQQQRPPPPASPLGGLGRVWPTREDLTREPAEEAPEPDPWGLEDEEGDEDVSLAQELLPPRVVESDTLINRRYMRATGLGALALLIAACRLIARRLRETRTTLKGSARRFNVDLFQVRLILG DCAFDC82206D661B vp1/2-ul25-3 2 5 unspecified role unspecified role neutral component neutral component gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component mcherry mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 1c vp1/2-dynactin-1 1 6 unspecified role unspecified role bait bait gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined s6 region required to bind necessary binding region required to bind range range certain certain sequence position certain s6-7 region sufficient to bind sufficient binding region sufficient to bind range range range range 4 unspecified role unspecified role prey prey physical association physical association 3 vp1/2-dynein-1 1 6 unspecified role unspecified role bait bait gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined s6 region required to bind necessary binding region required to bind range range certain certain sequence position certain s6-7 region sufficient to bind sufficient binding region sufficient to bind range range range range 3 unspecified role unspecified role prey prey physical association physical association 3