HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation. Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation. Virology (0042-6822) 2008 imex curation Lai C.K., Jeng K.S., Machida K., Cheng Y.S., Lai M.M. Virus - Publications including interactions involving viral proteins Origin of ATM taken for previous work of donating scientist (PMID:12556884) michlai@gate.sinica.edu.tw 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Lai C.K., Jeng K.S., Machida K., Cheng Y.S., Lai M.M. Virus - Publications including interactions involving viral proteins Origin of ATM taken for previous work of donating scientist (PMID:12556884) michlai@gate.sinica.edu.tw Accepted 2013-JUL-26 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Virology (0042-6822) 2008 imex curation Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation. Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation. Virology (0042-6822) 2008 imex curation Lai C.K., Jeng K.S., Machida K., Cheng Y.S., Lai M.M. Virus - Publications including interactions involving viral proteins Origin of ATM taken for previous work of donating scientist (PMID:12556884) michlai@gate.sinica.edu.tw 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy immunostaining immunostaining Lai C.K., Jeng K.S., Machida K., Cheng Y.S., Lai M.M. Virus - Publications including interactions involving viral proteins Origin of ATM taken for previous work of donating scientist (PMID:12556884) michlai@gate.sinica.edu.tw Accepted 2013-JUL-26 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Virology (0042-6822) 2008 imex curation Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation. Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation. Virology (0042-6822) 2008 imex curation Lai C.K., Jeng K.S., Machida K., Cheng Y.S., Lai M.M. Virus - Publications including interactions involving viral proteins Origin of ATM taken for previous work of donating scientist (PMID:12556884) michlai@gate.sinica.edu.tw 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lai C.K., Jeng K.S., Machida K., Cheng Y.S., Lai M.M. Virus - Publications including interactions involving viral proteins Origin of ATM taken for previous work of donating scientist (PMID:12556884) michlai@gate.sinica.edu.tw Accepted 2013-JUL-26 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Virology (0042-6822) 2008 imex curation Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation. Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation. Virology (0042-6822) 2008 imex curation Lai C.K., Jeng K.S., Machida K., Cheng Y.S., Lai M.M. Virus - Publications including interactions involving viral proteins Origin of ATM taken for previous work of donating scientist (PMID:12556884) michlai@gate.sinica.edu.tw 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lai C.K., Jeng K.S., Machida K., Cheng Y.S., Lai M.M. Virus - Publications including interactions involving viral proteins Origin of ATM taken for previous work of donating scientist (PMID:12556884) michlai@gate.sinica.edu.tw Accepted 2013-JUL-26 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Virology (0042-6822) 2008 imex curation Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation. Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation. Virology (0042-6822) 2008 imex curation Lai C.K., Jeng K.S., Machida K., Cheng Y.S., Lai M.M. Virus - Publications including interactions involving viral proteins Origin of ATM taken for previous work of donating scientist (PMID:12556884) michlai@gate.sinica.edu.tw 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Lai C.K., Jeng K.S., Machida K., Cheng Y.S., Lai M.M. Virus - Publications including interactions involving viral proteins Origin of ATM taken for previous work of donating scientist (PMID:12556884) michlai@gate.sinica.edu.tw Accepted 2013-JUL-26 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Virology (0042-6822) 2008 imex curation Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation. Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation. Virology (0042-6822) 2008 imex curation Lai C.K., Jeng K.S., Machida K., Cheng Y.S., Lai M.M. Virus - Publications including interactions involving viral proteins Origin of ATM taken for previous work of donating scientist (PMID:12556884) michlai@gate.sinica.edu.tw 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference confocal microscopy confocal microscopy immunostaining immunostaining Lai C.K., Jeng K.S., Machida K., Cheng Y.S., Lai M.M. Virus - Publications including interactions involving viral proteins Origin of ATM taken for previous work of donating scientist (PMID:12556884) michlai@gate.sinica.edu.tw Accepted 2013-JUL-26 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Virology (0042-6822) 2008 imex curation atm_human Serine-protein kinase ATM ATM Ataxia telangiectasia mutated protein protein human Homo sapiens Human MSLVLNDLLICCRQLEHDRATERKKEVEKFKRLIRDPETIKHLDRHSDSKQGKYLNWDAVFRFLQKYIQKETECLRIAKPNVSASTQASRQKKMQEISSLVKYFIKCANRRAPRLKCQELLNYIMDTVKDSSNGAIYGADCSNILLKDILSVRKYWCEISQQQWLELFSVYFRLYLKPSQDVHRVLVARIIHAVTKGCCSQTDGLNSKFLDFFSKAIQCARQEKSSSGLNHILAALTIFLKTLAVNFRIRVCELGDEILPTLLYIWTQHRLNDSLKEVIIELFQLQIYIHHPKGAKTQEKGAYESTKWRSILYNLYDLLVNEISHIGSRGKYSSGFRNIAVKENLIELMADICHQVFNEDTRSLEISQSYTTTQRESSDYSVPCKRKKIELGWEVIKDHLQKSQNDFDLVPWLQIATQLISKYPASLPNCELSPLLMILSQLLPQQRHGERTPYVLRCLTEVALCQDKRSNLESSQKSDLLKLWNKIWCITFRGISSEQIQAENFGLLGAIIQGSLVEVDREFWKLFTGSACRPSCPAVCCLTLALTTSIVPGTVKMGIEQNMCEVNRSFSLKESIMKWLLFYQLEGDLENSTEVPPILHSNFPHLVLEKILVSLTMKNCKAAMNFFQSVPECEHHQKDKEELSFSEVEELFLQTTFDKMDFLTIVRECGIEKHQSSIGFSVHQNLKESLDRCLLGLSEQLLNNYSSEITNSETLVRCSRLLVGVLGCYCYMGVIAEEEAYKSELFQKAKSLMQCAGESITLFKNKTNEEFRIGSLRNMMQLCTRCLSNCTKKSPNKIASGFFLRLLTSKLMNDIADICKSLASFIKKPFDRGEVESMEDDTNGNLMEVEDQSSMNLFNDYPDSSVSDANEPGESQSTIGAINPLAEEYLSKQDLLFLDMLKFLCLCVTTAQTNTVSFRAADIRRKLLMLIDSSTLEPTKSLHLHMYLMLLKELPGEEYPLPMEDVLELLKPLSNVCSLYRRDQDVCKTILNHVLHVVKNLGQSNMDSENTRDAQGQFLTVIGAFWHLTKERKYIFSVRMALVNCLKTLLEADPYSKWAILNVMGKDFPVNEVFTQFLADNHHQVRMLAAESINRLFQDTKGDSSRLLKALPLKLQQTAFENAYLKAQEGMREMSHSAENPETLDEIYNRKSVLLTLIAVVLSCSPICEKQALFALCKSVKENGLEPHLVKKVLEKVSETFGYRRLEDFMASHLDYLVLEWLNLQDTEYNLSSFPFILLNYTNIEDFYRSCYKVLIPHLVIRSHFDEVKSIANQIQEDWKSLLTDCFPKILVNILPYFAYEGTRDSGMAQQRETATKVYDMLKSENLLGKQIDHLFISNLPEIVVELLMTLHEPANSSASQSTDLCDFSGDLDPAPNPPHFPSHVIKATFAYISNCHKTKLKSILEILSKSPDSYQKILLAICEQAAETNNVYKKHRILKIYHLFVSLLLKDIKSGLGGAWAFVLRDVIYTLIHYINQRPSCIMDVSLRSFSLCCDLLSQVCQTAVTYCKDALENHLHVIVGTLIPLVYEQVEVQKQVLDLLKYLVIDNKDNENLYITIKLLDPFPDHVVFKDLRITQQKIKYSRGPFSLLEEINHFLSVSVYDALPLTRLEGLKDLRRQLELHKDQMVDIMRASQDNPQDGIMVKLVVNLLQLSKMAINHTGEKEVLEAVGSCLGEVGPIDFSTIAIQHSKDASYTKALKLFEDKELQWTFIMLTYLNNTLVEDCVKVRSAAVTCLKNILATKTGHSFWEIYKMTTDPMLAYLQPFRTSRKKFLEVPRFDKENPFEGLDDINLWIPLSENHDIWIKTLTCAFLDSGGTKCEILQLLKPMCEVKTDFCQTVLPYLIHDILLQDTNESWRNLLSTHVQGFFTSCLRHFSQTSRSTTPANLDSESEHFFRCCLDKKSQRTMLAVVDYMRRQKRPSSGTIFNDAFWLDLNYLEVAKVAQSCAAHFTALLYAEIYADKKSMDDQEKRSLAFEEGSQSTTISSLSEKSKEETGISLQDLLLEIYRSIGEPDSLYGCGGGKMLQPITRLRTYEHEAMWGKALVTYDLETAIPSSTRQAGIIQALQNLGLCHILSVYLKGLDYENKDWCPELEELHYQAAWRNMQWDHCTSVSKEVEGTSYHESLYNALQSLRDREFSTFYESLKYARVKEVEEMCKRSLESVYSLYPTLSRLQAIGELESIGELFSRSVTHRQLSEVYIKWQKHSQLLKDSDFSFQEPIMALRTVILEILMEKEMDNSQRECIKDILTKHLVELSILARTFKNTQLPERAIFQIKQYNSVSCGVSEWQLEEAQVFWAKKEQSLALSILKQMIKKLDASCAANNPSLKLTYTECLRVCGNWLAETCLENPAVIMQTYLEKAVEVAGNYDGESSDELRNGKMKAFLSLARFSDTQYQRIENYMKSSEFENKQALLKRAKEEVGLLREHKIQTNRYTVKVQRELELDELALRALKEDRKRFLCKAVENYINCLLSGEEHDMWVFRLCSLWLENSGVSEVNGMMKRDGMKIPTYKFLPLMYQLAARMGTKMMGGLGFHEVLNNLISRISMDHPHHTLFIILALANANRDEFLTKPEVARRSRITKNVPKQSSQLDEDRTEAANRIICTIRSRRPQMVRSVEALCDAYIILANLDATQWKTQRKGINIPADQPITKLKNLEDVVVPTMEIKVDHTGEYGNLVTIQSFKAEFRLAGGVNLPKIIDCVGSDGKERRQLVKGRDDLRQDAVMQQVFQMCNTLLQRNTETRKRKLTICTYKVVPLSQRSGVLEWCTGTVPIGEFLVNNEDGAHKRYRPNDFSAFQCQKKMMEVQKKSFEEKYEVFMDVCQNFQPVFRYFCMEKFLDPAIWFEKRLAYTRSVATSSIVGYILGLGDRHVQNILINEQSAELVHIDLGVAFEQGKILPTPETVPFRLTRDIVDGMGITGVEGVFRRCCEKTMEVMRNSQETLLTIVEVLLYDPLFDWTMNPLKALYLQQRPEDETELHPTLNADDQECKRNLSDIDQSFNKVAERVLMRLQEKLKGVEEGTVLSVGGQVNLLIQQAIDPKNLSRLFPGWKAWV DIP protein Q13315 original sequence version: 122 DIP protein Q13315 original sequence version: 189 DIP protein Q13315 original sequence version: 195 DIP protein Q13315 original sequence version: 166 DIP protein Q13315 original sequence version: 196 C0B4866E1E3199E2 ns3_ns4a_hcvco Serine protease NS3/Non-structural protein 4A protein protein hcvco Hepatitis C virus genotype 1b (isolate Con1) (HCV) ns3/4a-atm-1 5 ATM 7 unspecified role unspecified role neutral component neutral component NS3/4A 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 3a atm-ns3/4a-1 4 ATM 7 unspecified role unspecified role prey prey NS3/4A 8 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4a and b atm-ns3/4a-2 3 ATM 7 unspecified role unspecified role prey prey NS3/4A 8 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4c TRNS3/4A.36 cells atm-ns3/4a-3 6 ATM 7 unspecified role unspecified role neutral component neutral component flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NS3/4A 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 2b ns3/4a-atm-2 2 ATM 7 unspecified role unspecified role neutral component neutral component flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NS3/4A 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 2a atm-ns3/4a-4 1 ATM 7 unspecified role unspecified role neutral component neutral component flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NS3/4A 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 7