HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html The capsid and tegument of the alphaherpesviruses are linked by an interaction between the UL25 and VP1/2 proteins. The capsid and tegument of the alphaherpesviruses are linked by an interaction between the UL25 and VP1/2 proteins. Journal of virology 2007 imex curation Coller KE., Lee JI., Ueda A., Smith GA. Virus - Publications including interactions involving viral proteins g-smith3@northwestern.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed fluorescence imaging fluorescence microscopy fluorescence imaging tag visualisation tag visualisation Coller KE., Lee JI., Ueda A., Smith GA. Virus - Publications including interactions involving viral proteins g-smith3@northwestern.edu Accepted 2016-JUN-10 AT 12:10 BST AT 12:10 BST by MGT Journal of virology 2007 imex curation The capsid and tegument of the alphaherpesviruses are linked by an interaction between the UL25 and VP1/2 proteins. The capsid and tegument of the alphaherpesviruses are linked by an interaction between the UL25 and VP1/2 proteins. Journal of virology 2007 imex curation Coller KE., Lee JI., Ueda A., Smith GA. Virus - Publications including interactions involving viral proteins g-smith3@northwestern.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Coller KE., Lee JI., Ueda A., Smith GA. Virus - Publications including interactions involving viral proteins g-smith3@northwestern.edu Accepted 2016-JUN-10 AT 12:10 BST AT 12:10 BST by MGT Journal of virology 2007 imex curation q5ppb8_9alph UL36 protein protein pseudorabies virus pseudorabies virus MTADAVVVGYRNQYDPDLGPGSGVSCLRSSLSFLRLVFTNGVEAALTADAVDACLREGQAWTGPGGGMCAISELPNKIVYEGAGGAALHCVFSRVHGECNFFTPPAEGFQSTQITARAFFDGLWAQRAESFTLVVVGAVGLGLYRRGDVVYVFDPHGHGQITQAFVARLRPADVYAHVAGYAASELRWAGAFVFFLPHDQFAAVPALYGVGETYLVDEPYVERAVPSVHPMALPPDPAAAAPPTRGPGAIPVLAVAPVPAVPVSAAAAPAPAPGGAPLPPPPRVQKRPSMPKRRRPPWPASQEDLSSTHTVGGRPSRKFRPRTREPMGFAVRELELAPLDLGPLQAHADELEEHVVACAGRSLFPGRAGLLERFAELLFGHLQDFLVENGARTHGEAPCAVAALFEPILGALPERGPAGDFVAATHMVLTEARALLDVAGDDASAPVAKLALVAARVARPANEAMHRALDALESGGSYAQLSACFMDAAARDPGGVYDQTVVARARALFARARARESRESQRALERARDVSALEAALEAAHANFDAVDAGAPGAGGAAEPNTRDVKERAAKLPRKLALMVGDAIREYFLRGAQYSAKAMLMDRNGAQRFRVANAAVAGLRRLVDSLAAAEERLRALDAAPAAHDGALLAQLLEAGEDVSTDERLGAWFALLAEAQAHGHLERRELDTLTRDVTQINERAAKRASADADLARFDAMDAALAAAPDEETRVRAMEDLLRHIRAMGGHVLSQETRARLARRDAELRTLVQAAQQRAAEQNDLYMRLRALLGDFATLGPAVDLLAHITATPVPGTVLAALWRLVARYRELLTSDPGALVGAGQPLGALVNALADNPPITRFFLAHADAVAAALADADTAEKAARAAGVLRAAVEHPELGFLEPLARQCEERAAALRESERVAALQAALSAAVAAAVEMLGRLRAQPDEGTLVEARHLALEVARAAAVEEPQLQKMALAARQRVGELEAAVAAVEARRAAEVERAQRQLWARAADAALRRAEQHAFDAAEFVRLRDVAADRGYDAAELRPAAERALGANAETVTTALDAVFAFNPHLPENAGAAAGPPLQALQRLTWLDDYAVAAPVIGSLFGADTEPLSRLARIAGGVLARAAAGMGRFSYYDAVGFVGADMAHVPRLAKYVDFYRRGFPEYEAELAALGARRADVLQAGGSRGAEICRALEEVTYVRAAADAARALDEGVHLHVPSTELLRGAVTYLERFDFARFERTAYAEAMRALVRRDLAAARDGLAAAEADQAAATERATRLLREVVDAAAARDRDGALELANLKNLLRVTPPPASLNIDRAASAEELVTQAALLLGAVEEAPELDVQAVEWLHQARLIIDTHPLTTRVDGRGPMDEFAERIEALQTLRQEADTLRQRLTAAEVAWDEAWEDFGRARGRAGASEDEAPLAEARARALQTATMEVLALRSDAVYPRLPAKFVGRIESKYSARAGALGDFFRDAGAVEEDVRELDRMAARIGPELRHDALRALAAAFDARAERLPRWIPPKYAAYRELLTLRLQFYAAYGGAGPTPARPRASTAPCARLKQRVAARLGDRDVICTVREARSDADAVFPTVFLDDNNVPVEYQVCYRAVGDKLAAMLCTDEGAELRPPLTEGLMETATVAGMRLMNEIIHLRLNYEKARDTGFATFARFVRHRRADWDSAAKAAAETYAAVLATTLSRTFGALDRVAHPPLEPAVRLALPDLMVAAVAGEPTHLFNFTRVDLAGQHEYMAKTLDAVLDRALDAVDTLDAGARPPRRPLRDGQALFTLRVADWTEGRFSETELWAPWAHTETGQRLARAVPGALATFTVLARMCIPPRLLSALWSALQPEANAAYDAAVTERLDAAAHVREAAALGAPGAGDLYAPVGTGMTFTVTGSPPSSATRVSAMDLAAAAVLVGAPLVIAMENLDVYSPGSGLTLAVTLCDLRDPALHRGVSTDLSSWGERLLALDDNPIENACLAPQLERLSALIAARPLAGEPACLVLVDSQLAVSRVLWAPPEPPRRPGFTLEDDPLLREIPFLETTEDQLPEMDPADPLFTSIIMGDARVDPATSEELYGRPSFRNLGDPFPYAVSATPTRPAAPIPGLPPELAGEDDGFVPDDDFGANPWLDLLGDDLVASALPLPPSPPVDPPTLPPLAPAAPPAPPPAKPAELTPAAKLAPPAPPPAKPVETTPLTKPQPQPQQGPPAAHKKPAAGTKAAAAPKQQPREPAPKPHSSPRKIQPSLKARIEPPPPVINPPYPATAPAPETAPPEAPQAQPPAAAKPTPQPQGPPPPPQPPSAQAPPAQKPPAQPATAAATTAPKATPQTQPPTRAQTQTAPPPPSAATAAAQVPPQPPSSQPAAKPRGAPPAPPAPPPPSAQTTLPRPAAPPAPPPPSAQTTLPRPAPPPPSAPAATPTPPAPGPAPSAKKSDGDRIVEPKAGAPPDVRDAKFGGKVAGAASPGRPLDDERYKAGRADKCEHTVGPDQHRTRSPPGTSDSEPASASSSAASTPSREAASSSSSPAGTPSDGARGGPPSAAGAAARAPPGAPGSGGQRRGDGGPAADASRGDQPAGHLPAAPVAQPLPSAVLRTKARTSVGIRDHSRRKSMALVPRRPVSAPRQTGGLPPAAQPEGRPPGGLEPRPPQAQAQAPATAPTGPPAAAAAAATAAVPAPGVGPGGPVRRAAGPRPPAGHLRLHIPPPQPVQLEGVVVPLPASPETPAPAQTQPPRSPLAAPTSLAAPSEIERPAASAAAAAAATTTTTSSSSATTSSAPAAPPPAPPPSRPAAPPPAPPPSRPAPTPERHGEPPRLMFTPPPLPPQPSQQRPPEAPWTWPEPRDSAGPPALFTPPPSDPPVQFPGREEPPLPPPPPPQPLWASEGEAETPATPLRSPLERMPWPPPLEAPGTTPELPPEFAPPAPRSPLADPHQPPAWFDDLTALVPPAHPPPRRQQQRPPPPASPLGGLGRVWPTREDLTREPAEEAPELDPWGLEDEEGDEDGDEDVSLAQELLPPRVVESDTLINRRYMRATGLGALALLIAACRLIARRLRETRTTLKGSARRFNVDLFQVRLILG ECAD9E1E3DC22D1A ltp_hhv11 Large tegument protein deneddylase UL36 protein protein hhv11 Human herpesvirus 1 (strain 17) (HHV-1) Human herpes simplex virus 1 HHV-1 MGGGNNTNPGGPVHKQAGSLASRAHMIAGTPPHSTMERGGDRDIVVTGARNQFAPDLEPGGSVSCMRSSLSFLSLIFDVGPRDVLSAEAIEGCLVEGGEWTRATAGPGPPRMCSIVELPNFLEYPGARGGLRCVFSRVYGEVGFFGEPAAGLLETQCPAHTFFAGPWALRPLSYTLLTIGPLGMGLFRDGDTAYLFDPHGLPEGTPAFIAKVRAGDMYPYLTYYTRDRPDVRWAGAMVFFVPSGPEPAAPADLTAAALHLYGASETYLQDEAFSERRVAITHPLRGEIAGLGEPCVGVGPREGVGGPGPHPPTAAQSPPPTRARRDDRASETSRGTAGPSAKPEAKRPNRAPDDVWAVALKGTPPTDPPSADPPSADPPSAIPPPPPSAPKTPAAEAAEEDDDDMRVLEMGVVPVGRHRARYSAGLPKRRRPTWTPPSSVEDLTSGEKTKRSAPPAKTKKKSTPKGKTPVGAAVPASVPEPVLASAPPDPAGPPVAEAGEDDGPTVPASSQALEALKTRRSPEPPGADLAQLFEAHPNVAATAVKFTACSAALAREVAACSRLTISALRSPYPASPGLLELCVIFFFERVLAFLIENGARTHTQAGVAGPAAALLEFTLNMLPWKTAVGDFLASTRLSLADVAAHLPLVQHVLDENSLIGRLALAKLILVARDVIRETDAFYGELADLELQLRAAPPANLYTRLGEWLLERSQAHPDTLFAPATPTHPEPLLYRVQALAKFARGEEIRVEAEDRQMREALDALARGVDAVSQHAGPLGVMPAPAGAAPQGAPRPPPLGPEAVQVRLEEVRTQARRAIEGAVKEYFYRGAVYSAKALQASDNNDRRFHVASAAVVPVVQLLESLPVFDQHTRDIAQRAAIPAPPPIATSPTAILLRDLIQRGQTLDAPEDLAAWLSVLTDAANQGLIERKPLDELARSIRDINDQQARRSSGLAELRRFDALDAALGQQLDSDAAFVPAPGASPYPDDGGLSPEATRMAEEALRQARAMDAAKLTAELAPDARARLRERARSLEAMLEGARERAKVARDAREKFLHKLQGVLRPLPDFVGLKACPAVLATLRASLPAGWSDLPEAVRGAPPEVTAALRADMWGLLGQYRDALEHPTPDTATALSGLHPSFVVVLKNLFADAPETPFLLQFFADHAPIIAHAVSNAINAGSAAVATADPASTVDAAVRAHRVLVDAVTALGAAASDPASPLAFLAAMADSAAGYVKATRLALDARVAIAQLTTLGSAAADLVVQVRRAANQPEGEHASLIQAATRATTGARESLAGHEGRFGGLLHAEGTAGDHSPSGRALQELGKVIGATRRRADELEAATADLREKMAAQRARSSHERWAADVEAVLDRVESGAEFDVVELRRLQALAGTHGYNPRDFRKRAEQALGTNAKAVTLALETALAFNPYTPENQRHPMLPPLAAIHRIDWSAAFGAAADTYADMFRVDTEPLARLLRLAGGLLERAQANDGFIDYHEAVLHLSEDLGGVPALRQYVPFFQKGYAEYVDIRDRLDALRADARRAIGSVALDLAAAAEEISAVRNDPAAAAELVRAGVTLPCPSEDALVACVAALERVDQSPVKDTAYAHYVAFVTRQDLADTKDAVVRAKQQRAEATERVTAGLREVLAARERRAQLEAEGLANLKTLLKVVAVPATVAKTLDQARSAEEIADQVEILVDQTEKARELDVQAVAWLEHAQRTFETHPLSAASGDGPGLLTRQGARLQALFDTRRRVEALRRSLEEAEAEWDEVWGRFGRVRGGAWKSPEGFRAACEQLRALQDTTNTVSGLRAQRDYERLPAKYQGVLGAKSAERAGAVEELGGRVAQHADLSARLRDEVVPRVAWEMNFDTLGGLLAEFDAVAGDLAPWAVEEFRGARELIQRRMGLYSAYAKATGQTGAGAAAAPAPLLVDLRALDARARASAPPGQEADPQMLRRRGEAYLRVSGGPGPLVLREATSTLDRPFAPSFLVPDGTPLQYALCFPAVTDKLGALLMCPEAACIRPPLPTDTLESASTVTAMYVLTVINRLQLALSDAQAANFQLFGRFVRHRQARWGASMDAAAELYVALVATTLTREFGCRWAQLEWGGDAAAPGPPLGPQSSTRHRVSFNENDVLVALVASSPEHIYTFWRLDLVRQHEYMHLTLPRAFQNAADSMLFVQRLTPHPDARIRVLPAFSAGGPPTRGLMFGTRLADWRRGKLSETDPLAPWRSVPELGTERGAALGKLSPAQALAAVSVLGRMCLPSTALVALWTCMFPDDYTEYDSFDALLTARLESGQTLSPSGGREASPPAPPNALYRPTGQHVAVPAAATHRTPAARVTAMDLVLAAVLLGAPVVVALRNTTAFSRESELELCLTLFDSRARGPDAALRDAVSSDIETWAVRLLHADLNPIENACLAAQLPRLSALIAERPLARGPPCLVLVDISMTPVAVLWENPDPPGPPDVRFVGSEATEELPFVAGGEDVLAASATDEDPFLARAILGRPFDASLLSGELFPGHPVYQRAPDDQSPSVPNPTPGPVDLVGAEGSLGPGSLAPTLFTDATPGEPVPPRMWAWIHGLEELASDDSGGPAPLLAPDPLSPTADQSVPTSQCAPRPPGPAVTAREARPGVPAESTRPAPVGPRDDFRRLPSPQSSPAPPDATAPRPPASSRASAASSSGSRARRHRRARSLARATQASATTQGWRPPALPDTVAPVTDFARPPAPPKPPEPAPHALVSGVPLPLGPQAAGQASPALPIDPVPPPVATGTVLPGGENRRPPLTSGPAPTPPRVPVGGPQRRLTRPAVASLSESRESLPSPWDPADPTAPVLGRNPAEPTSSSPAGPSPPPPAVQPVAPPPTSGPPPTYLTLEGGVAPGGPVSRRPTTRQPVATPTTSARPRGHLTVSRLSAPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQNGHVAPGEYPAVRFRAPQNRPSVPASASSTNPRTGSSLSGVSSWASSLALHIDATPPPVSLLQTLYVSDDEDSDATSLFLSDSEAEALDPLPGEPHSPITNEPFSALSADDSQEVTRLQFGPPPVSANAVLSRRYVQRTGRSALAVLIRACYRLQQQLQRTRRALLHHSDAVLTSLHHVRMLLG Tegument protein mint CC5D31FF4F9FE3F4 cvc2_hhv11 Capsid vertex component 2 CVC2 UL25 protein protein hhv11 Human herpesvirus 1 (strain 17) (HHV-1) Human herpes simplex virus 1 HHV-1 MDPYCPFDALDVWEHRRFIVADSRNFITPEFPRDFWMSPVFNLPRETAAEQVVVLQAQRTAAAAALENAAMQAAELPVDIERRLRPIERNVHEIAGALEALETAAAAAEEADAARGDEPAGGGDGGAPPGLAVAEMEVQIVRNDPPLRYDTNLPVDLLHMVYAGRGATGSSGVVFGTWYRTIQDRTITDFPLTTRSADFRDGRMSKTFMTALVLSLQACGRLYVGQRHYSAFECAVLCLYLLYRNTHGAADDSDRAPVTFGDLLGRLPRYLACLAAVIGTEGGRPQYRYRDDKLPKTQFAAGGGRYEHGALASHIVIATLMHHGVLPAAPGDVPRDASTHVNPDGVAHHDDINRAAAAFLSRGHNLFLWEDQTLLRATANTITALGVIQRLLANGNVYADRLNNRLQLGMLIPGAVPSEAIARGASGSDSGAIKSGDNNLEALCANYVLPLYRADPAVELTQLFPGLAALCLDAQAGRPVGSTRRVVDMSSGARQAALVRLTALELINRTRTNPTPVGEVIHAHDALAIQYEQGLGLLAQQARIGLGSNTKRFSAFNVSSDYDMLYFLCLGFIPQYLSAV DIP protein P10209 original sequence version: 59 3F8F0B7C122B2E36 q83419_9alph UL25 protein protein pseudorabies virus pseudorabies virus MDRAWFAFEAAAIPGSARHFIAPPFPVGFWARPGFSEGLDARLALAHANARRRAAAAALDNAMAAGARLEAEVDEQLRPLERQVERVAEALVVLEETARAAEEADAARAAEEAPEATAAADEGREVQIAKNDVALAYDANLSLDFLAMVYAARGAASGVLFGTWYATLQATLVAERPQVSRAIDSRDGRMSRTFMGVTTTALQACGRLYVGNRHYSALESAALCLHLVHRVRQGAGAAGAAAPLGIADLLERVPEYLDALSQALAEGGRISYRYNYPRVPREQLHGRYALEGHSVLAALARLRVVPGANVGANEVDGAGFVDEVNRAAAAFLGRGQNLFLGEDAPLLRATVNTITGLLLLRRLLHNGNVYGDRLRNNFQLGALVPNAPPPRGASGDAPASRSGDGNLRFLLAHYVVVAYRADERTELTQLFPGLAALALDAHSIRARVQRHQLNLVRLVALELQNRQRVTAPVNEVIAAHDAVAVQYEEGLGLLLQQPHLRNAADKRLGQFGVSSDYDLLYFLCLGYIPQFAAA 9BBA7F78AF9644E0 vp1/2-ul25-1 2 6 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait n terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cbd region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 4A-C vp1/2-ul25-2 2 4 unspecified role unspecified role prey prey n terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cbd region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role bait bait n terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4E ul36-ul25-1 1 6 unspecified role unspecified role neutral component neutral component n terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role neutral component neutral component n terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range cbd region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization 2