MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it A conserved pathway to activate BRCA1-dependent ubiquitylation at DNA damage sites. A conserved pathway to activate BRCA1-dependent ubiquitylation at DNA damage sites. EMBO J. (0261-4189) 2006 imex curation Polanowska J., Martin JS., Garcia-Muse T., Petalcorin MI., Boulton SJ. Virus - Publications including interactions involving viral proteins 2009-01-08 Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Polanowska J., Martin JS., Garcia-Muse T., Petalcorin MI., Boulton SJ. Virus - Publications including interactions involving viral proteins EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation A conserved pathway to activate BRCA1-dependent ubiquitylation at DNA damage sites. A conserved pathway to activate BRCA1-dependent ubiquitylation at DNA damage sites. EMBO J. (0261-4189) 2006 imex curation Polanowska J., Martin JS., Garcia-Muse T., Petalcorin MI., Boulton SJ. Virus - Publications including interactions involving viral proteins 2009-01-08 Only protein-protein interactions caeel Caenorhabditis elegans tap tandem affinity purification TAP TAP tap predetermined predetermined participant predetermined Polanowska J., Martin JS., Garcia-Muse T., Petalcorin MI., Boulton SJ. Virus - Publications including interactions involving viral proteins EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation A conserved pathway to activate BRCA1-dependent ubiquitylation at DNA damage sites. A conserved pathway to activate BRCA1-dependent ubiquitylation at DNA damage sites. EMBO J. (0261-4189) 2006 imex curation Polanowska J., Martin JS., Garcia-Muse T., Petalcorin MI., Boulton SJ. Virus - Publications including interactions involving viral proteins 2009-01-08 Only protein-protein interactions caeel Caenorhabditis elegans anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Polanowska J., Martin JS., Garcia-Muse T., Petalcorin MI., Boulton SJ. Virus - Publications including interactions involving viral proteins EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation h4_human Histone H4 H4-16 H4C1 H4C11 H4C12 H4C13 H4C14 H4C15 H4C2 H4C3 H4C4 H4C5 H4C6 H4C8 H4C9 H4/A H4/B H4/C H4/D H4/E H4/G H4/H H4/I H4/J H4/K H4/M H4/N H4/O H4F2 H4FA H4FB H4FC H4FD H4FE H4FG H4FH H4FI H4FJ H4FK H4FM H4FN H4FO HIST1H4A HIST1H4B HIST1H4C HIST1H4D HIST1H4E HIST1H4F HIST1H4H HIST1H4I HIST1H4J HIST1H4K HIST1H4L HIST2H4 HIST2H4A HIST2H4B HIST4H4 protein protein human Homo sapiens Human MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG DIP protein P62805 original sequence version: 68 DIP protein P62805 original sequence version: 111 DIP protein P62805 original sequence version: 94 DIP protein P62805 original sequence version: 97 DIP protein P62805 original sequence version: 116 DIP protein P62805 original sequence version: 105 DIP protein P62805 original sequence version: 120 DIP protein P62805 original sequence version: 144 DIP protein P62805 original sequence version: 150 DIP protein P62805 original sequence version: 154 DIP protein P62805 original sequence version: 156 DIP protein P62805 original sequence version: 112 DIP protein P62805 original sequence version: 121 DIP protein P62805 original sequence version: 123 DIP protein P62805 original sequence version: 126 DIP protein P62805 original sequence version: 132 DIP protein P62805 original sequence version: 136 DIP protein P62805 original sequence version: 119 A9E5DFD3F8B97598 brca1_human Breast cancer type 1 susceptibility protein BRCA1 RNF53 RING finger protein 53 RING-type E3 ubiquitin transferase BRCA1 protein protein human Homo sapiens Human MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLGTVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGSSVSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAGSKETCNDRRTPSTEKKVDLNADPLCERKEWNKQKLPCSENPRDTEDVPWITLNSSIQKVNEWFSRSDELLGSDDSHDGESESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNIEDKIFGKTYRKKASLPNLSHVTENLIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENKTKGDSIQNEKNPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFAPFSNPGNAEEECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRGNETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKEASSSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQKGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKASQEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQSMDSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY DIP protein P38398 original sequence version: 183 DIP protein P38398 original sequence version: 111 DIP protein P38398 original sequence version: 149 DIP protein P38398-1 original sequence version: 149 DIP protein P38398 original sequence version: 142 DIP protein P38398 original sequence version: 212 DIP protein P38398 original sequence version: 163 DIP protein P38398 original sequence version: 185 DIP protein P38398 original sequence version: 148 DIP protein P38398 original sequence version: 210 89C6D83FF56312AF bard1_caeel BRCA1-associated RING domain protein 1 brd-1 RING-type E3 ubiquitin transferase BARD1 K04C2.4 protein protein caeel Caenorhabditis elegans MFENTKKALETFRTAIECVKCKKPRGDLQYLGSSCKHAYCWECIATFQQKPSGKRSSVARHMCPSCAFPLDTSKITEAHMLKTCFDTLSELNDLLQKVGTTSLTQAEFACTQNIFNKEKTPADAVEKFLETQAHMPDEMGQLGEEDDDLMCKDENRENSNSPELDIFHDYSKEASPTRNSTKRPSTVSVHERKPKRSSILKTSVKNEPAAPVVDLFASQVPQRTHQNDLLTPFIERRSTAPAATGVATYAQAFGSSSNPVKAEIIEEDIFSKAIPLTKRQASMSASAKKQPKLEPEEPEEVPSTSRSRKNSIKSDKIERRSQSPMSFGEKSMSVKSEQRRSSYGTRRGEAVLVNSIRNNRIPQLRSAVEAGTCVNEKEDGKTPLYVAVENSSLEAVKILVEAGAVINASCGSTLETTLHEAVRRQNTQIVEYLLSKGASIKIRNIAGKTVEEMAKSDPKIRKIIEKFKTEQRVLQPVVAPPKSRLHFVQLIDEKMLTESEKRKLPGKINIVPADMDSPTHVVVTVDLKTRVLNINKEHIGEILKAIIKSGMIVSRDWLRACIIDPSKVDDDRSYMVQKVRWMEGEVFENTIEQWKKTITKMQPKLFAGCKFFIPKPKYNFLDRPALFEIIRSAGGQAAAREPIIDEKDPPPYHNANLKPNFVLYSLTHDIGDKFRDCTKYNLVSEQWLIEAILGCSITTPPH g8jy83_caeel ubq-1 F25B5.4 CELE_F25B5.4 protein protein caeel Caenorhabditis elegans MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLIGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGVYSSFGAPSQRRYADLRQDFDWKNHHSRGRSFGHH The sequence has been withdrawn from uniprot. mint 2B44B7AECD41713B q95q25_caeel DNA repair protein RAD51 homolog rad-51 Y43C5A.6 CELE_Y43C5A.6 protein protein caeel Caenorhabditis elegans MGQSWGYEGIAKRSLCTHKWLYNLNLHSINLFLPIESKMSAQASRQKKSDQEQRAADQALLNAAIEDNAMEQDENFTVIDKLESSGISSGDISKLKEAGYYTYESLAFTTRRELRNVKGISDQKAEKIMKEAMKFVQMGFTTGAEVHVKRSQLVQIRTGSASLDRLLGGGIETGSITEVYGEYRTGKTQLCHSLAVLCQLPIDMGGGEGKCMYIDTNATFRPERIIAIAQRYNMDSAHVLENIAVARAYNSEHLMALIIRAGAMMSESRYAVVIVDCATAHFRNEYTGRGDLAERQMKLSAFLKCLAKLADEYGVAVIITNQVVAQVDGGASMFQADAKKPIGGHIIAHMSTTRLYLRKGKGENRVAKMVQSPNLPEAEATYSITNHGIEDARED E844516B0C1A6AF8 q93335_caeel uncharacterized protein brc-1 brc-1 C36A4.8 protein protein caeel Caenorhabditis elegans MADVALRITETVARLQKELKCGICCSTYKDPILSTCFHIFCRSCINACFERKRKVQCPICRSVLDKRSCRDTYQITMAVQNYLKLSEAFKKDIENMNTFKSLPPEKMFMESQMPLDITIIPENDGKRCAPDFAIPFLPVRRKRPSRPQPPSAFAEEPAEPVEPPEPATKQPVELQSRVFPLEKLKKDVETSTETYKISREELKNVDIEEYINTLRENSTEIDEIDALFQLMPTMRQFLRNNINQLMEKFHVAPPKKSEKPANRRVSFASSQDLENIKIMTASESLETPPEPIQKLAQKPEVFKSTQNLIDLNLNTAVKKPVVVASDDDEVVEDSEGELQIDEDDLANVTCATSVRNIGKSLCAEYIREGRSISQKSTAYLYAIARKCVIVGRQWLVDCITTGLLLSEADYTITSCSSTIPVKIPPSIGSEMGWLRSRNDEHGKLFAGRRFMILRKFTMNPYFDYKQLIELVQQCGGEILSCYENLSPEKLYIIFSKHSKAIEESKNIENLYKCDVVTMEWVLDSISEYLILPTQPYKAVDSIGCLQD mint The sequence has been withdrawn from uniprot. E38C6CA6B94F068B brca1-hist1h4a 1 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F4A F4C EMBO-Journal Under normal growth conditions, BRCA1 immuno-precipitates from soluble and chromatin bound MCF7 cell extracts contain very low levels of UbcH5c (Figure 4A, lanes 1 and 3, respectively). In contrast, following IR-treatment, UbcH5c is significantly increased in BRCA1 immuno-precipitates from chromatin bound MCF7 extracts (Figure 4A, lane 4). homomint mint brd_1-brc_1 2 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 6 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F1C EMBO-Journal we have biochemically characterized CeBRD-1 associated proteins (CeBCD complexes) purified by tandem immuno-affinity (Polanowska et al, 2004) (Figure 1A). mint brd_1-rad_51 2 6 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell multiple tagged tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F1C EMBO-Journal we have biochemically characterized CeBRD-1 associated proteins (CeBCD complexes) purified by tandem immuno-affinity (Polanowska et al, 2004) (Figure 1A). mint ubq_1-brd_1 3 7 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F1F EMBO-Journal CeBRD-1 is ubiquitylated in the CeBCD complex mint