MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Raf-1 kinase associates with Hepatitis C virus NS5A and regulates viral replication. Raf-1 kinase associates with Hepatitis C virus NS5A and regulates viral replication. FEBS Lett. (0014-5793) 2006 imex curation Bürckstümmer T., Kriegs M., Lupberger J., Pauli EK., Schmittel S., Hildt E. Virus - Publications including interactions involving viral proteins tbuerckstuemmer@cemm.oeaw.ac.at Only protein-protein interactions spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bürckstümmer T., Kriegs M., Lupberger J., Pauli EK., Schmittel S., Hildt E. Virus - Publications including interactions involving viral proteins tbuerckstuemmer@cemm.oeaw.ac.at accepted FEBS Lett. (0014-5793) 2006 Only protein-protein interactions imex curation Raf-1 kinase associates with Hepatitis C virus NS5A and regulates viral replication. Raf-1 kinase associates with Hepatitis C virus NS5A and regulates viral replication. FEBS Lett. (0014-5793) 2006 imex curation Bürckstümmer T., Kriegs M., Lupberger J., Pauli EK., Schmittel S., Hildt E. Virus - Publications including interactions involving viral proteins tbuerckstuemmer@cemm.oeaw.ac.at Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Bürckstümmer T., Kriegs M., Lupberger J., Pauli EK., Schmittel S., Hildt E. Virus - Publications including interactions involving viral proteins tbuerckstuemmer@cemm.oeaw.ac.at accepted FEBS Lett. (0014-5793) 2006 Only protein-protein interactions imex curation Raf-1 kinase associates with Hepatitis C virus NS5A and regulates viral replication. Raf-1 kinase associates with Hepatitis C virus NS5A and regulates viral replication. FEBS Lett. (0014-5793) 2006 imex curation Bürckstümmer T., Kriegs M., Lupberger J., Pauli EK., Schmittel S., Hildt E. Virus - Publications including interactions involving viral proteins tbuerckstuemmer@cemm.oeaw.ac.at Only protein-protein interactions human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Bürckstümmer T., Kriegs M., Lupberger J., Pauli EK., Schmittel S., Hildt E. Virus - Publications including interactions involving viral proteins tbuerckstuemmer@cemm.oeaw.ac.at accepted FEBS Lett. (0014-5793) 2006 Only protein-protein interactions imex curation raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF DIP protein P04049 original sequence version: 117 DIP protein P04049 original sequence version: 106 DIP protein P04049 original sequence version: 114 DIP protein P04049 original sequence version: 141 DIP protein P04049 original sequence version: 142 DIP protein P04049 original sequence version: 147 DIP protein P04049-1 original sequence version: 136 DIP protein P04049 original sequence version: 136 DIP protein P04049 original sequence version: 153 DIP protein P04049 original sequence version: 150 DIP protein P04049 original sequence version: 164 DIP protein P04049 original sequence version: 172 DIP protein P04049-1 original sequence version: 172 DIP protein P04049 original sequence version: 184 DIP protein P04049 original sequence version: 188 DIP protein P04049 original sequence version: 195 EF821B5349711BC3 o39474_9hepc NS5A protein protein 9hepc Hepatitis C virus MGSGSWLKDVWDWICTVLADLKTWLQSKLLPRLPGVPFLSCQRGYKGVWRGDGIMNTTCPCAAQITGHVKNGSMRIVGPKTCSNTWHGTFPTNAYTTGPCTPSPAPNYSRALWRVAAEEYVEVTRVGDFHYVTGMTTDNVKCPCQVPAPEFFTEVDGVRLHRYAPACKPLLRDEVVFQVGLNQFPVGSQLPCEPEPDVAVLTSMLTDPSHITAETAKRKLARGSPPSLASSSASQLSAPSLKATCTTRHDSPDADLIEANLLWRQEMGGNITRVESENKVVILDSFDPLHAEEDEKEVSVPAEILRKSKRFPPALPIWARPDYNPPLLEPWKDPDYVPPVVHGCPLPPNKAPPIPPPRRKRTVVLTESTVSSALAELATKTFGSSGSSAVDSGTASAPPDQSTDNGDTGSDVESYSSMPPLEGEPGDPDLSDGSWSTVSEEAGEDVVCCSMS mint 46172D3E6C24EEF7 ns5a-raf1 1 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 physical association physical association F1B FEBS-Letters homomint mint raf1-ns5a 1 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 physical association physical association F1C FEBS-Letters homomint mint ns5a-raf1-1 3 5 immunostaining immunostaining predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain range MINT-1503959: original sequence: 211-449 : ITAETAKRKLARGSPPSLASSSASQLSAPSLKATCTTRHDSPDADLIEANLLWRQEMGGNITRVESENKVVILDSFDPLHAEEDEKEVSVPAEILRKSKRFPPALPIWARPDYNPPLLEPWKDPDYVPPVVHGCPLPPNKAPPIPPPRRKRTVVLTESTVSSALAELATKTFGSSGSSAVDSGTASAPPDQSTDNGDTGSDVESYSSMPPLEGEPGDPDLSDGSWSTVSEEAGEDVVC ha tagged ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 4 immunostaining immunostaining predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell Stoichiometry: 1.0 colocalization colocalization F2C FEBS-Letters homomint mint ns5a-raf1-2 2 5 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 4 polyclonal western polyclonal antibody western blot polyclonal western predetermined predetermined participant predetermined unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F1A FEBS-Letters homomint mint