HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Protein kinase C-related kinase 2 regulates hepatitis C virus RNA polymerase function by phosphorylation. Protein kinase C-related kinase 2 regulates hepatitis C virus RNA polymerase function by phosphorylation. J. Biol. Chem. (0021-9258) 2004 imex curation Kim S.J., Kim J.H., Kim Y.G., Lim H.S., Oh J.W. Virus - Publications including interactions involving viral proteins jwoh@yonsei.ac.kr 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Kim S.J., Kim J.H., Kim Y.G., Lim H.S., Oh J.W. Virus - Publications including interactions involving viral proteins The human hepatoma stable cell line Huh7TR expressing the tetracycline repressor and transfected with pcDNA4.0-HCVns (for the Huh7TR-NS cell line) was used. jwoh@yonsei.ac.kr Accepted 2015-JAN-15 by MGT J. Biol. Chem. (0021-9258) 2004 imex curation Protein kinase C-related kinase 2 regulates hepatitis C virus RNA polymerase function by phosphorylation. Protein kinase C-related kinase 2 regulates hepatitis C virus RNA polymerase function by phosphorylation. J. Biol. Chem. (0021-9258) 2004 imex curation Kim S.J., Kim J.H., Kim Y.G., Lim H.S., Oh J.W. Virus - Publications including interactions involving viral proteins jwoh@yonsei.ac.kr 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim S.J., Kim J.H., Kim Y.G., Lim H.S., Oh J.W. Virus - Publications including interactions involving viral proteins Figure 2B - The human hepatoma stable cell line Huh7TR expressing the tetracycline repressor and transfected with pcDNA4.0-HCVns (for the Huh7TR-NS cell line) was used. Figure 2C - Huh7 cell line expressing HCV subgenomic RNA, R-1, was used. jwoh@yonsei.ac.kr Accepted 2015-JAN-15 by MGT J. Biol. Chem. (0021-9258) 2004 imex curation Protein kinase C-related kinase 2 regulates hepatitis C virus RNA polymerase function by phosphorylation. Protein kinase C-related kinase 2 regulates hepatitis C virus RNA polymerase function by phosphorylation. J. Biol. Chem. (0021-9258) 2004 imex curation Kim S.J., Kim J.H., Kim Y.G., Lim H.S., Oh J.W. Virus - Publications including interactions involving viral proteins jwoh@yonsei.ac.kr 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim S.J., Kim J.H., Kim Y.G., Lim H.S., Oh J.W. Virus - Publications including interactions involving viral proteins jwoh@yonsei.ac.kr Accepted 2015-JAN-15 by MGT J. Biol. Chem. (0021-9258) 2004 imex curation Protein kinase C-related kinase 2 regulates hepatitis C virus RNA polymerase function by phosphorylation. Protein kinase C-related kinase 2 regulates hepatitis C virus RNA polymerase function by phosphorylation. J. Biol. Chem. (0021-9258) 2004 imex curation Kim S.J., Kim J.H., Kim Y.G., Lim H.S., Oh J.W. Virus - Publications including interactions involving viral proteins jwoh@yonsei.ac.kr 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Kim S.J., Kim J.H., Kim Y.G., Lim H.S., Oh J.W. Virus - Publications including interactions involving viral proteins A monoclonal anti-M13 antibody was used to detect the amount of bound C1 phage. jwoh@yonsei.ac.kr Accepted 2015-JAN-15 by MGT J. Biol. Chem. (0021-9258) 2004 imex curation Protein kinase C-related kinase 2 regulates hepatitis C virus RNA polymerase function by phosphorylation. Protein kinase C-related kinase 2 regulates hepatitis C virus RNA polymerase function by phosphorylation. J. Biol. Chem. (0021-9258) 2004 imex curation Kim S.J., Kim J.H., Kim Y.G., Lim H.S., Oh J.W. Virus - Publications including interactions involving viral proteins jwoh@yonsei.ac.kr 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro phage display phage display nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kim S.J., Kim J.H., Kim Y.G., Lim H.S., Oh J.W. Virus - Publications including interactions involving viral proteins jwoh@yonsei.ac.kr Accepted 2015-JAN-15 by MGT J. Biol. Chem. (0021-9258) 2004 imex curation Protein kinase C-related kinase 2 regulates hepatitis C virus RNA polymerase function by phosphorylation. Protein kinase C-related kinase 2 regulates hepatitis C virus RNA polymerase function by phosphorylation. J. Biol. Chem. (0021-9258) 2004 imex curation Kim S.J., Kim J.H., Kim Y.G., Lim H.S., Oh J.W. Virus - Publications including interactions involving viral proteins jwoh@yonsei.ac.kr 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Kim S.J., Kim J.H., Kim Y.G., Lim H.S., Oh J.W. Virus - Publications including interactions involving viral proteins jwoh@yonsei.ac.kr Accepted 2015-JAN-15 by MGT J. Biol. Chem. (0021-9258) 2004 imex curation sc1 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) TSTAGRIVRRAI pkn2_human Serine/threonine-protein kinase N2 PKN2 PRK2 PRKCL2 Protein kinase C-like 2 Protein-kinase C-related kinase 2 PKN gamma protein protein human Homo sapiens Human MASNPERGEILLTELQGDSRSLPFSENVSAVQKLDFSDTMVQQKLDDIKDRIKREIRKELKIKEGAENLRKVTTDKKSLAYVDNILKKSNKKLEELHHKLQELNAHIVVSDPEDITDCPRTPDTPNNDPRCSTSNNRLKALQKQLDIELKVKQGAENMIQMYSNGSSKDRKLHGTAQQLLQDSKTKIEVIRMQILQAVQTNELAFDNAKPVISPLELRMEELRHHFRIEFAVAEGAKNVMKLLGSGKVTDRKALSEAQARFNESSQKLDLLKYSLEQRLNEVPKNHPKSRIIIEELSLVAASPTLSPRQSMISTQNQYSTLSKPAALTGTLEVRLMGCQDILENVPGRSKATSVALPGWSPSETRSSFMSRTSKSKSGSSRNLLKTDDLSNDVCAVLKLDNTVVGQTSWKPISNQSWDQKFTLELDRSRELEISVYWRDWRSLCAVKFLRLEDFLDNQRHGMCLYLEPQGTLFAEVTFFNPVIERRPKLQRQKKIFSKQQGKTFLRAPQMNINIATWGRLVRRAIPTVNHSGTFSPQAPVPTTVPVVDVRIPQLAPPASDSTVTKLDFDLEPEPPPAPPRASSLGEIDESSELRVLDIPGQDSETVFDIQNDRNSILPKSQSEYKPDTPQSGLEYSGIQELEDRRSQQRFQFNLQDFRCCAVLGRGHFGKVLLAEYKNTNEMFAIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVMEYAAGGDLMMHIHTDVFSEPRAVFYAACVVLGLQYLHEHKIVYRDLKLDNLLLDTEGFVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGASEKDAEDVKKHPFFRLIDWSALMDKKVKPPFIPTIRGREDVSNFDDEFTSEAPILTPPREPRILSEEEQEMFRDFDYIADWC 687EC417A0F51C1D o92972-pro_0000278753 RNA-directed RNA polymerase protein protein hcvj4 Hepatitis C virus genotype 1b (strain HC-J4) (HCV) SMSYTWTGALITPCAAEESKLPINPLSNSLLRHHNMVYATTSRSASLRQKKVTFDRLQVLDDHYRDVLKEMKAKASTVKAKLLSIEEACKLTPPHSAKSKFGYGAKDVRNLSSRAVNHIRSVWEDLLEDTETPIDTTIMAKSEVFCVQPEKGGRKPARLIVFPDLGVRVCEKMALYDVVSTLPQAVMGSSYGFQYSPKQRVEFLVNTWKSKKCPMGFSYDTRCFDSTVTESDIRVEESIYQCCDLAPEARQAIRSLTERLYIGGPLTNSKGQNCGYRRCRASGVLTTSCGNTLTCYLKATAACRAAKLQDCTMLVNGDDLVVICESAGTQEDAAALRAFTEAMTRYSAPPGDPPQPEYDLELITSCSSNVSVAHDASGKRVYYLTRDPTTPLARAAWETARHTPINSWLGNIIMYAPTLWARMILMTHFFSILLAQEQLEKALDCQIYGACYSIEPLDLPQIIERLHGLSAFTLHSYSPGEINRVASCLRKLGVPPLRTWRHRARSVRAKLLSQGGRAATCGRYLFNWAVRTKLKLTPIPAASQLDLSGWFVAGYSGGDIYHSLSRARPRWFPLCLLLLSVGVGIYLLPNR 2420 3010 E4716928C4EB0840 ns5b-pkn2-2 5 8 unspecified role unspecified role prey prey Q16513:p.Leu520Ile mutation mutation certain certain sequence position certain certain certain sequence position certain L I Wed May 02 14:41:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu520ile' Tue Jun 30 18:55:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu520Ile' region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association No figure ns5b-prk2-1 2 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 2B-C ns5b-prk2-2 1 8 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization 3 pkn2-ns5b-1 3 8 unspecified role unspecified role bait bait human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 9 unspecified role unspecified role prey prey finger_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 4B and 7B p27958_pro-pkn2-3 6 8 enzyme enzyme unspecified role unspecified role human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 9 enzyme target enzyme target substrate substrate unspecified role unspecified role finger_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation autophosphorylation of immunoprecipitated PRK2 was significantly inhibited by a PRK2-specific inhibitor HA1077 with a concomitant inhibition of NS5B phosphorylation. 5A, 6B and 7C p27958_pro-pkn2-2 4 9 unspecified role unspecified role bait bait n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey Q16513:p.Leu520Ile mutation mutation certain certain sequence position certain certain certain sequence position certain L I Wed May 02 18:14:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu520ile' Tue Jun 30 16:03:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu520Ile' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1B pkn2-pkn2-1 6 8 putative self putative self unspecified role unspecified role human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation true autophosphorylation of immunoprecipitated PRK2 was significantly inhibited by a PRK2-specific inhibitor HA1077. 5A, 6B p27958_pro-pkn2-1 4 7 competitor competitor competitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role bait bait n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey Q16513:p.Leu520Ile mutation mutation certain certain sequence position certain certain certain sequence position certain L I Wed May 02 17:51:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu520ile' Tue Jun 30 18:43:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu520Ile' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain association association 1B p27958_pro-sc1-1 4 7 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role bait bait n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 1B