IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Nucleocapsid protein of SARS coronavirus tightly binds to human cyclophilin A. Nucleocapsid protein of SARS coronavirus tightly binds to human cyclophilin A. Biochemical and biophysical research communications 2004 imex curation Luo C., Luo H., Zheng S., Gui C., Yue L., Yu C., Sun T., He P., Chen J., Shen J., Luo X., Li Y., Liu H., Bai D., Shen J., Yang Y., Li F., Zuo J., Hilgenfeld R., Pei G., Chen K., Shen X., Jiang H. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus xshen@mail.shcnc.ac.cn,hljiang@mail.shcnc.ac.cn Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Luo C., Luo H., Zheng S., Gui C., Yue L., Yu C., Sun T., He P., Chen J., Shen J., Luo X., Li Y., Liu H., Bai D., Shen J., Yang Y., Li F., Zuo J., Hilgenfeld R., Pei G., Chen K., Shen X., Jiang H. Virus - Publications including interactions involving viral proteins xshen@mail.shcnc.ac.cn,hljiang@mail.shcnc.ac.cn Accepted 2020-MAY-18 AT 10:59 BST AT 10:59 BST by MGT Biochemical and biophysical research communications 2004 Only protein-protein interactions imex curation imex curation ncap_sars Nucleoprotein N 9a Nucleocapsid protein protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA mint Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid 43FC8750F1253034 ppia_human Peptidyl-prolyl cis-trans isomerase A Cyclophilin A Cyclosporin A-binding protein PPIA CYPA Rotamase A protein protein human Homo sapiens Human MVNPTVFFDIAVDGEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTRHNGTGGKSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITIADCGQLE DIP protein P62937 original sequence version: 81 DIP protein P62937-1 original sequence version: 141 9B2E637A555E4434 n-ppia-3 1 2 unspecified role unspecified role prey prey purified purified P59595:p.Gln307Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q D Tue Jun 30 15:55:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln307Asp' P59595:p.Ile305Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I A Tue Jun 30 18:38:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile305Ala' P59595:p.Gln307Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q A Tue Jun 30 17:47:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln307Ala' n-teminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P59595:p.Trp302Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain W A Tue Jun 30 18:14:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp302Ala' ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role bait bait purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1, Fig 1 ppia-n-1 1 2 unspecified role unspecified role bait bait purified purified n-teminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey purified purified P62937:p.Arg55Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R D Tue Jun 30 18:38:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg55Asp' P62937:p.Gln63Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q A Tue Jun 30 18:42:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln63Ala' P62937:p.Arg69Lys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R K Tue Jun 30 19:42:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg69Lys' n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P62937:p.Arg55Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 19:04:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg55Ala' P62937:p.Trp121Phe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain W F Tue Jun 30 18:06:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp121Phe' P62937:p.Arg69Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 16:03:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg69Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1, Fig 1