IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Interaction between heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: implications for virus fusogenic mechanism and identification of fusion inhibitors. Interaction between heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: implications for virus fusogenic mechanism and identification of fusion inhibitors. Lancet (London, England) 2004 imex curation Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus SJiang@NYBloodcenter.org 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel experimental particp experimental participant identification experimental particp Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins SJiang@NYBloodcenter.org Accepted 2020-JUL-24 AT 11:02 BST AT 11:02 BST by LUANA Lancet (London, England) 2004 Only protein-protein interactions imex curation imex curation Interaction between heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: implications for virus fusogenic mechanism and identification of fusion inhibitors. Interaction between heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: implications for virus fusogenic mechanism and identification of fusion inhibitors. Lancet (London, England) 2004 imex curation Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus SJiang@NYBloodcenter.org 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins SJiang@NYBloodcenter.org Accepted 2020-JUL-24 AT 11:02 BST AT 11:02 BST by LUANA Lancet (London, England) 2004 Only protein-protein interactions imex curation imex curation Interaction between heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: implications for virus fusogenic mechanism and identification of fusion inhibitors. Interaction between heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: implications for virus fusogenic mechanism and identification of fusion inhibitors. Lancet (London, England) 2004 imex curation Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus SJiang@NYBloodcenter.org 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins SJiang@NYBloodcenter.org Accepted 2020-JUL-24 AT 11:02 BST AT 11:02 BST by LUANA Lancet (London, England) 2004 Only protein-protein interactions imex curation imex curation Interaction between heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: implications for virus fusogenic mechanism and identification of fusion inhibitors. Interaction between heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: implications for virus fusogenic mechanism and identification of fusion inhibitors. Lancet (London, England) 2004 imex curation Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus SJiang@NYBloodcenter.org 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins SJiang@NYBloodcenter.org Accepted 2020-JUL-24 AT 11:02 BST AT 11:02 BST by LUANA Lancet (London, England) 2004 Only protein-protein interactions imex curation imex curation Interaction between heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: implications for virus fusogenic mechanism and identification of fusion inhibitors. Interaction between heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: implications for virus fusogenic mechanism and identification of fusion inhibitors. Lancet (London, England) 2004 imex curation Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus SJiang@NYBloodcenter.org 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro cd circular dichroism CD CD cd predetermined predetermined participant predetermined Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins SJiang@NYBloodcenter.org Accepted 2020-JUL-24 AT 11:02 BST AT 11:02 BST by LUANA Lancet (London, England) 2004 Only protein-protein interactions imex curation imex curation Interaction between heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: implications for virus fusogenic mechanism and identification of fusion inhibitors. Interaction between heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: implications for virus fusogenic mechanism and identification of fusion inhibitors. Lancet (London, England) 2004 imex curation Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus SJiang@NYBloodcenter.org 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins SJiang@NYBloodcenter.org Accepted 2020-JUL-24 AT 11:02 BST AT 11:02 BST by LUANA Lancet (London, England) 2004 Only protein-protein interactions imex curation imex curation spike_sars Spike glycoprotein S E2 Peplomer protein 2 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT DIP protein P59594 original sequence version: 48 s-s-6 3 7 unspecified role unspecified role prey prey NP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role bait bait CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 2, bottom. s-s-7 5 7 unspecified role unspecified role neutral component neutral component NP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role neutral component neutral component CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 3, top. s-s-8 6 7 unspecified role unspecified role neutral component neutral component NP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role neutral component neutral component CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 4, top s-s-9 1 7 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component NP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 7 weight by comassie molecular weight estimation by coomasie staining weight by comassie tag perox activity tag visualisation by peroxidase activity tag perox activity experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 4, middle and bottom s-s-10 4 7 unspecified role unspecified role neutral component neutral component NP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role neutral component neutral component biotin tag biotin tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Figure 5, Table 3 s-s-11 4 7 weight by comassie molecular weight estimation by coomasie staining weight by comassie tag perox activity tag visualisation by peroxidase activity tag perox activity predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component biotin tag biotin tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Figure 5, Table 3 s-26 2 7 weight by comassie molecular weight estimation by coomasie staining weight by comassie tag perox activity tag visualisation by peroxidase activity tag perox activity predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Figure 6 s-s-12 2 7 unspecified role unspecified role neutral component neutral component NP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role neutral component neutral component CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Figure 6