HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Direct interaction of TFIIB and the IE protein of equine herpesvirus 1 is required for maximal trans-activation function. Direct interaction of TFIIB and the IE protein of equine herpesvirus 1 is required for maximal trans-activation function. Virology 2003 imex curation Albrecht RA., Jang HK., Kim SK., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins docall@lsuhsc.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Albrecht RA., Jang HK., Kim SK., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins docall@lsuhsc.edu Accepted 2016-JUN-02 AT 10:14 BST AT 10:14 BST by ORCHARD Virology 2003 imex curation Direct interaction of TFIIB and the IE protein of equine herpesvirus 1 is required for maximal trans-activation function. Direct interaction of TFIIB and the IE protein of equine herpesvirus 1 is required for maximal trans-activation function. Virology 2003 imex curation Albrecht RA., Jang HK., Kim SK., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins docall@lsuhsc.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Albrecht RA., Jang HK., Kim SK., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins docall@lsuhsc.edu Accepted 2016-JUN-02 AT 10:14 BST AT 10:14 BST by ORCHARD Virology 2003 imex curation Direct interaction of TFIIB and the IE protein of equine herpesvirus 1 is required for maximal trans-activation function. Direct interaction of TFIIB and the IE protein of equine herpesvirus 1 is required for maximal trans-activation function. Virology 2003 imex curation Albrecht RA., Jang HK., Kim SK., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins docall@lsuhsc.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Albrecht RA., Jang HK., Kim SK., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins docall@lsuhsc.edu Accepted 2016-JUN-02 AT 10:14 BST AT 10:14 BST by ORCHARD Virology 2003 imex curation icp4_ehv1k Major viral transcription factor IE 155 kDa immediate-early protein protein protein ehv1k Equine herpesvirus 1 (strain Kentucky A) EHV-1 Equine abortion virus MASQRSDFAPDLYDFIESNDFGEDPLIRAASAAEEGFTQPAAPDLLYGSQNMFGVDDAPLSTPVVVIPPPSPAPEPRGGKAKRSPSAAGSGGPPTPAAAAQPASPAPSPAPGLAAMLKMVHSSVAPGNGRRATGSSSPGGGDAADPVALDSDTETCPGSPQPEFPSSASPGGGSPAPRVRSISISSSSSSSSSMDEDDQADGAGASSSSSSSSDDSDSDEGGEEETPRPRHSQNAAKTPSAAGSPGPSSGGDRPAAGAATPKSCRSGAASPGAPAPAPASAPAPSRPGGGLLPPGARILEYLEGVREANLAKTLERPEPPAGMASPPGRSPHRLPKDQRPKSALAGASKRKRANPRPRPQTQTQAPAEEAPQTAVWDLLDMNSSQATGAAAAAASAPAAASCAPGVYQREPLLTPSGDPWPGSDPPPMGRVRYGGTGDSRDGLWDDPEIVLAASRYAEAQAPVPVFVPEMGDSTKQYNALVRMVFESREAMSWLQNSKLSGQDQNLAQFCQKFIHAPRGHGSFITGSVANPLPHIGDAMAAGNALWALPHAAASVAMSRRYDRTQKSFILQSLRRAYADMAYPRDEAGRPDSLAAVAGCPAQAAAAAASQQQPEAPAPSVRVREAYTRVCAALGPRRKAAAAAAAPGTRAPRPSAFRLRELGDACVLACQAVFEALLRLRGGASAVPGLDPSEIPSPACPPEALCSNPAGLETAALSLYELRDLVERARLLGDSDPTHRLGSDELRLAVRAVLVVARTVAPLVRYNAEGARARASAWTVTQAVFSIPSLVGGMLGEAVSLLAPPTRSQQPSSSSPGGEPFSGSAAAEGSLQTLPPLWPTVPGKQSATVPSSHSQSPQHSQSGGGAGATTATCCRATQTNARSRGQQHQPQKARSPQAAASPAHLSQEAMPGSSSDDRAIHGRPRGKSGKRRSEPLEPAAQAGASASFSSSARGYDPSGPVDSPPAPKRRVATPGHQAPRALGPMPAEGPDRRGGFRRVPRGDCHTPRPSDAACAAYCPPELVAELIDNQLFPEAWRPALTFDPQALATIAARCSGPPARDGARLGELAASGPLRRRAAWMHQIPDPEDVKVVVLYSPLQDEDLLGGLPASRPGGSRREPLWSDLKGGLSALLAALGNRILTKRSHAWAGNWTGAPDVSALNAQGVLLLSTGDLAFTGCVEYLCLRLGSARRKLLVLDAVSTEDWPQDGPAISQYHIYMRAALTPRVACAVRWPGERHLSRAVLTSSTLFGPGLFARAEAAFARLYPDSAPLRLCRSSNVAYTVDTRAGERTRVPLAPREYRQRVLPDYDGCKDMRAQAEGLGFHDPDFEEGAAQSHRAANRWGLGAWLRPVYLACGRRGAGAVEPSELLIPELLSEFCRVALLEPDAEAEPLVLPITEAPRRRAPRVDWEPGFGSRSTSVLHMGATELCLPEPDDELEIDGAGDVELVVEHPGPSPGVAQALRRAPIKIEVVSDDEDGGDWCNPYLS 044E39A570608A6B tf2b_human Transcription initiation factor IIB GTF2B TF2B TFIIB General transcription factor TFIIB S300-II protein protein human Homo sapiens Human MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL DIP protein Q00403 original sequence version: 119 DIP protein Q00403 original sequence version: 181 9CC7E102526C2722 ie-tfiib-1 3 5 unspecified role unspecified role prey prey purified purified in vitro In vitro 4 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 1 ie-gtf2b-1 1 5 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined IR2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association 2A-D and 4 gtf2b-ie-1 2 5 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey mouse-lm mouse fibroblast L-M cells Mouse lm Murine fibroblast L-M cells physical association physical association 2E gtf2b-ie-2 1 5 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 4 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 3