HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Direct interaction between nucleolin and hepatitis C virus NS5B. Direct interaction between nucleolin and hepatitis C virus NS5B. J. Biol. Chem. (0021-9258) 2003 imex curation Hirano M., Kaneko S., Yamashita T., Luo H., Qin W., Shirota Y., Nomura T., Kobayashi K., Murakami S. Virus - Publications including interactions involving viral proteins semuraka@kenroku.ipc.kanazawa-u. ac.jp 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human Homo sapiens Human confocal microscopy confocal microscopy immunostaining immunostaining Hirano M., Kaneko S., Yamashita T., Luo H., Qin W., Shirota Y., Nomura T., Kobayashi K., Murakami S. Virus - Publications including interactions involving viral proteins semuraka@kenroku.ipc.kanazawa-u. ac.jp Accepted 2014-FEB-07 by ORCHARD J. Biol. Chem. (0021-9258) 2003 imex curation Direct interaction between nucleolin and hepatitis C virus NS5B. Direct interaction between nucleolin and hepatitis C virus NS5B. J. Biol. Chem. (0021-9258) 2003 imex curation Hirano M., Kaneko S., Yamashita T., Luo H., Qin W., Shirota Y., Nomura T., Kobayashi K., Murakami S. Virus - Publications including interactions involving viral proteins semuraka@kenroku.ipc.kanazawa-u. ac.jp 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cv-1 Monkey African Green kidney fibroblast Grivet cv-1 Monkey African Green kidney fibroblast pull down pull down western blot western blot Immuno blot Immuno blot Hirano M., Kaneko S., Yamashita T., Luo H., Qin W., Shirota Y., Nomura T., Kobayashi K., Murakami S. Virus - Publications including interactions involving viral proteins semuraka@kenroku.ipc.kanazawa-u. ac.jp Accepted 2014-FEB-07 by ORCHARD J. Biol. Chem. (0021-9258) 2003 imex curation Direct interaction between nucleolin and hepatitis C virus NS5B. Direct interaction between nucleolin and hepatitis C virus NS5B. J. Biol. Chem. (0021-9258) 2003 imex curation Hirano M., Kaneko S., Yamashita T., Luo H., Qin W., Shirota Y., Nomura T., Kobayashi K., Murakami S. Virus - Publications including interactions involving viral proteins semuraka@kenroku.ipc.kanazawa-u. ac.jp 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Hirano M., Kaneko S., Yamashita T., Luo H., Qin W., Shirota Y., Nomura T., Kobayashi K., Murakami S. Virus - Publications including interactions involving viral proteins semuraka@kenroku.ipc.kanazawa-u. ac.jp Accepted 2014-FEB-07 by ORCHARD J. Biol. Chem. (0021-9258) 2003 imex curation h6u5q1_chlae Nucleolin protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MVKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQKKGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAKAVAIPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEEDDSEEDDEDDEDEDEDEDEIEPAVMKAAAAAPASEDEDDEDDEDDEDEDDDDEEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEEDDEDEDDDDDDDEDDEDEDDDEDEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEGTEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLPYKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNSTWSGESKTLVLSNLSYSATEETLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE A229C4E7B006C324 p27958-pro_0000037577 RNA-directed RNA polymerase protein protein HCV77 Hepatitis C virus genotype 1a (isolate H77) SMSYSWTGALVTPCAAEEQKLPINALSNSLLRHHNLVYSTTSRSACQRKKKVTFDRLQVLDSHYQDVLKEVKAAASKVKANLLSVEEACSLAPPHSAKSKFGYGAKDVRCHARKAVAHINSVWKDLLEDSVTPIDTTIMAKNEVFCVQPEKGGRKPARLIVFPDLGVRVCEKMALYDVVSKLPLAVMGSSYGFQYSPGQRVEFLVQAWKSKKTPMGLSYDTRCFDSTVTESDIRTEEAIYQCCDLDPQARVAIKSLTERLYVGGPLTNSRGENCGYRRCRASRVLTTSCGNTLTRYIKARAACRAAGLQDCTMLVCGDDLVVICESAGVQEDAASLRAFTEAMTRYSAPPGDPPQPEYDLELITSCSSNVSVAHDGAGKRVYYLTRDPTTPLARAAWETARHTPVNSWLGNIIMFAPTLWARMILMTHFFSVLIARDQLEQALNCEIYGACYSIEPLDLPPIIQRLHGLSAFSLHSYSPGEINRVAACLRKLGVPPLRAWRHRAWSVRARLLARGGKAAICGKYLFNWAVRTKLKLTPITAAGRLDLSGWFTAGYSGGDIYHSVSHARPRWFWFCLLLLAAGVGIYLLPNR 2421 3011 2F89B19E29A81A02 nucl_human Nucleolin NCL Protein C23 protein protein human Homo sapiens Human MVKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQKKGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAKAVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDDDDDEEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEEDDEDEDDDDDEDDEDDDDEDDEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEGTEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLPYKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNSTWSGESKTLVLSNLSYSATEETLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE DIP protein P19338 original sequence version: 93 DIP protein P19338 original sequence version: 123 C97F6E34E5CA6727 ncl-ns5b-3 1 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 1A ncl-ns5b-4 1 5 unspecified role unspecified role neutral component neutral component gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 1B and 5 ncl-ns5b-1 2 5 unspecified role unspecified role unspecified role unspecified role region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 23 20 physical association physical association Fig. 2A and 3B ncl-ns5b-2 3 5 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2B ns5b-ncl-1 3 5 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 3C and 4