HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html The NS5A protein of bovine viral diarrhoea virus interacts with the alpha subunit of translation elongation factor-1. The NS5A protein of bovine viral diarrhoea virus interacts with the alpha subunit of translation elongation factor-1. J. Gen. Virol. (0022-1317) 2001 imex curation Johnson C.M., Perez D.R., French R., Merrick W.C., Donis R.O. Virus - Publications including interactions involving viral proteins rdonis!unlnotes.unl.edu 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Johnson C.M., Perez D.R., French R., Merrick W.C., Donis R.O. Virus - Publications including interactions involving viral proteins Interactions between eEF1A and NS5A of BVDV strains 890, CV24 Oregon, NCP7 (GenBank accession nos U18059, AF091605 and U63479, respectively) was also shown in Figure 4B. rdonis!unlnotes.unl.edu Accepted 2014-MAY-14 by ORCHARD J. Gen. Virol. (0022-1317) 2001 imex curation The NS5A protein of bovine viral diarrhoea virus interacts with the alpha subunit of translation elongation factor-1. The NS5A protein of bovine viral diarrhoea virus interacts with the alpha subunit of translation elongation factor-1. J. Gen. Virol. (0022-1317) 2001 imex curation Johnson C.M., Perez D.R., French R., Merrick W.C., Donis R.O. Virus - Publications including interactions involving viral proteins rdonis!unlnotes.unl.edu 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Johnson C.M., Perez D.R., French R., Merrick W.C., Donis R.O. Virus - Publications including interactions involving viral proteins rdonis!unlnotes.unl.edu Accepted 2014-MAY-14 by ORCHARD J. Gen. Virol. (0022-1317) 2001 imex curation The NS5A protein of bovine viral diarrhoea virus interacts with the alpha subunit of translation elongation factor-1. The NS5A protein of bovine viral diarrhoea virus interacts with the alpha subunit of translation elongation factor-1. J. Gen. Virol. (0022-1317) 2001 imex curation Johnson C.M., Perez D.R., French R., Merrick W.C., Donis R.O. Virus - Publications including interactions involving viral proteins rdonis!unlnotes.unl.edu 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Johnson C.M., Perez D.R., French R., Merrick W.C., Donis R.O. Virus - Publications including interactions involving viral proteins rdonis!unlnotes.unl.edu Accepted 2014-MAY-14 by ORCHARD J. Gen. Virol. (0022-1317) 2001 imex curation The NS5A protein of bovine viral diarrhoea virus interacts with the alpha subunit of translation elongation factor-1. The NS5A protein of bovine viral diarrhoea virus interacts with the alpha subunit of translation elongation factor-1. J. Gen. Virol. (0022-1317) 2001 imex curation Johnson C.M., Perez D.R., French R., Merrick W.C., Donis R.O. Virus - Publications including interactions involving viral proteins rdonis!unlnotes.unl.edu 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Johnson C.M., Perez D.R., French R., Merrick W.C., Donis R.O. Virus - Publications including interactions involving viral proteins rdonis!unlnotes.unl.edu Accepted 2014-MAY-14 by ORCHARD Bovine cDNA library (B42 MDBK) J. Gen. Virol. (0022-1317) 2001 imex curation The NS5A protein of bovine viral diarrhoea virus interacts with the alpha subunit of translation elongation factor-1. The NS5A protein of bovine viral diarrhoea virus interacts with the alpha subunit of translation elongation factor-1. J. Gen. Virol. (0022-1317) 2001 imex curation Johnson C.M., Perez D.R., French R., Merrick W.C., Donis R.O. Virus - Publications including interactions involving viral proteins rdonis!unlnotes.unl.edu 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Johnson C.M., Perez D.R., French R., Merrick W.C., Donis R.O. Virus - Publications including interactions involving viral proteins Interactions between eEF1A and NS5A of BVDV strains 890, CV24 Oregon, NCP7 (GenBank accession nos U18059, AF091605 and U63479, respectively) was also shown in Figure 4B. rdonis!unlnotes.unl.edu Accepted 2014-MAY-14 by ORCHARD J. Gen. Virol. (0022-1317) 2001 imex curation ef1a1_rabit Elongation factor 1-alpha 1 EEF1A1 EEF1A EF1A Eukaryotic elongation factor 1 A-1 Elongation factor Tu protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK mint This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis D465615545AF686A p19711-pro_0000038035 Non-structural protein 5A protein protein bvdvn Bovine viral diarrhea virus (isolate NADL) SGNYILDLIYGLHKQINRGLKKMVLGWAPAPFSCDWTPSDERIRLPTDNYLRVETRCPCGYEMKAFKNVGGKLTKVEESGPFLCRNRPGRGPVNYRVTKYYDDNLREIKPVAKLEGQVEHYYKGVTAKIDYSKGKMLLATDKWEVEHGVITRLAKRYTGVGFNGAYLGDEPNHRALVERDCATITKNTVQFLKMKKGCAFTYDLTISNLTRLIELVHRNNLEEKEIPTATVTTWLAYTFVNEDVGTIKPVLGERVIPDPVVDINLQPEVQVDTSEVGITIIGRETLMTTGVTPVLEKVEPDASDNQNSVKIGLDEGNYPGPGIQTHTLTEEIHNRDARPFIMILGSRNSISNRAKTARNINLYTGNDPREIRDLMAAGRMLVVALRDVDPELSEMVDFKGTFLDREALEALSLGQPKPKQVTKEAVRNLIEQKKDVEIPNWFASDDPVFLEVALKNDKYYLVGDVGELKDQAKALGATDQTRIIKEVGSRTYAMKL 2774 3269 2D05DE6E50D12869 q96662-pro_0000038022 Non-structural protein 5A protein protein bvdvc Bovine viral diarrhea virus (strain CP7) SGNYVLDLIYSLHKQINRGLKKIVLGWAPAPFSCDWTPSDERIRLPTNNYLRVETKCPCGYEMKALRNVGGSLTKVEEKGPFLCRNRLGRGPVNYRVTKYYDDNLKEIKPVAKLEGFVDHYYKGVTARIDYGRGKMLLATDKWEVEHGVVTRLAKRYTGVGFKGAYLGDEPNHRDLVERDCATITKNTVQFLKMKKGCAFTYDLTLSNLTRLIELVHKNNLEEKDIPAATVTTWLAYTFVNEDIGTIKPVLGERVVTDPVVDVNLQPEVQVDTSEVGITLVGRAALMTTGTTPVVEKTEPNADGGPSSIKIGLDEGRYPGPGLQDRTLTDEIHSRDERPFVLVLGSKNSMSNRAKTARNINLYKGNNPREIRDLMAQGRMLVVALKDFNPELSELVDFKGTFLDREALEALSLGRPKSKQVTTATVRELLEQEVQVEIPSWFGAGDPVFLEVTLKGDRYHLVGDVDRVKDQAKELGATDQTRIVKEVGARTYTMKL 2693 3188 922055C80A6CFFD1 ef1a1_bovin Elongation factor 1-alpha 1 EEF1A1 EEF1A EF1A Eukaryotic elongation factor 1 A-1 Elongation factor Tu protein protein bovin Bos taurus (Bovine) Bovine MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK D465615545AF686A ef1a1_chlae_protein Elongation factor 1-alpha 1 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey ns5a-eef1a-5 4 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association No figure eef1a1-ns5a-1 1 9 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cv-1 Monkey African Green kidney fibroblast Grivet cv-1 Monkey African Green kidney fibroblast physical association physical association Fig. 2B eef1a1-ns5a-2 1 7 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cv-1 Monkey African Green kidney fibroblast Grivet cv-1 Monkey African Green kidney fibroblast physical association physical association Fig. 2B ns5a-eef1a1-1 2 9 unspecified role unspecified role prey prey 35s tag 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 7 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2D eef1a1-ns5a-3 3 7 unspecified role unspecified role unspecified role unspecified role 9 unspecified role unspecified role unspecified role unspecified role physical association physical association No figure eef1a1-ns5a-4 3 8 unspecified role unspecified role unspecified role unspecified role 9 unspecified role unspecified role unspecified role unspecified role physical association physical association No figure eef1a1-ns5a-5 1 9 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cv-1 Monkey African Green kidney fibroblast Grivet cv-1 Monkey African Green kidney fibroblast physical association physical association Fig. 4B eef1a1-ns5a-6 1 9 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cv-1 Monkey African Green kidney fibroblast Grivet cv-1 Monkey African Green kidney fibroblast physical association physical association Fig. 4B ef1a1_ns5a-1 5 6 unspecified role unspecified role prey prey in vitro In vitro 7 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig. 2C ns5a-eef1a1-3 5 10 unspecified role unspecified role prey prey chlae-cv-1 Monkey African Green kidney fibroblast Grivet cv-1 Monkey African Green kidney fibroblast 7 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 2C