HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html The EICP22 protein of equine herpesvirus 1 physically interacts with the immediate-early protein and with itself to form dimers and higher-order complexes. The EICP22 protein of equine herpesvirus 1 physically interacts with the immediate-early protein and with itself to form dimers and higher-order complexes. Journal of virology 2000 imex curation Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU 04-Aug-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU DOCALL @LSUMC.EDU Accepted 2016-JUL-26 AT 10:04 BST AT 10:04 BST by ORCHARD Journal of virology 2000 imex curation The EICP22 protein of equine herpesvirus 1 physically interacts with the immediate-early protein and with itself to form dimers and higher-order complexes. The EICP22 protein of equine herpesvirus 1 physically interacts with the immediate-early protein and with itself to form dimers and higher-order complexes. Journal of virology 2000 imex curation Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU 04-Aug-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU DOCALL @LSUMC.EDU Accepted 2016-JUL-26 AT 10:04 BST AT 10:04 BST by ORCHARD Journal of virology 2000 imex curation The EICP22 protein of equine herpesvirus 1 physically interacts with the immediate-early protein and with itself to form dimers and higher-order complexes. The EICP22 protein of equine herpesvirus 1 physically interacts with the immediate-early protein and with itself to form dimers and higher-order complexes. Journal of virology 2000 imex curation Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU 04-Aug-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-lm mouse fibroblast L-M cells Mouse lm Murine fibroblast L-M cells crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins dithiobis(succinimidylpropionate) used as cross linker DOCALL@LSUMC.EDU DOCALL @LSUMC.EDU Accepted 2016-JUL-26 AT 10:04 BST AT 10:04 BST by ORCHARD Journal of virology 2000 imex curation The EICP22 protein of equine herpesvirus 1 physically interacts with the immediate-early protein and with itself to form dimers and higher-order complexes. The EICP22 protein of equine herpesvirus 1 physically interacts with the immediate-early protein and with itself to form dimers and higher-order complexes. Journal of virology 2000 imex curation Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU 04-Aug-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation horse-tcc horse Horse Horse tcc transitional cell carcinoma cells confocal microscopy confocal microscopy immunostaining immunostaining Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU DOCALL @LSUMC.EDU Accepted 2016-JUL-26 AT 10:04 BST AT 10:04 BST by ORCHARD Journal of virology 2000 imex curation The EICP22 protein of equine herpesvirus 1 physically interacts with the immediate-early protein and with itself to form dimers and higher-order complexes. The EICP22 protein of equine herpesvirus 1 physically interacts with the immediate-early protein and with itself to form dimers and higher-order complexes. Journal of virology 2000 imex curation Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU 04-Aug-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-lm mouse fibroblast L-M cells Mouse lm Murine fibroblast L-M cells anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU DOCALL @LSUMC.EDU Accepted 2016-JUL-26 AT 10:04 BST AT 10:04 BST by ORCHARD Journal of virology 2000 imex curation The EICP22 protein of equine herpesvirus 1 physically interacts with the immediate-early protein and with itself to form dimers and higher-order complexes. The EICP22 protein of equine herpesvirus 1 physically interacts with the immediate-early protein and with itself to form dimers and higher-order complexes. Journal of virology 2000 imex curation Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU 04-Aug-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU DOCALL @LSUMC.EDU Accepted 2016-JUL-26 AT 10:04 BST AT 10:04 BST by ORCHARD Journal of virology 2000 imex curation pp11_yeast Serine/threonine-protein phosphatase PP1-1 SIT4 PPH1 YDL047W D2693 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVSRGPDEWLETIKKCQALTENEMKQLCEMVKELLMEESNIQPVQTPVTVCGDIHGQFHDLLELFRTAGGFPDDINYIFLGDYVDRGYYSLETFTLLMCLKVKYPAKITLVRGNHESRQITQVYGFYEECLNKYGSTTVWKYCCQVFDFLTLAAIIDGKILCVHGGLSPEIRMLDQIRVLSRAQEVPHEGGFSDLLWSDPDNVEAWQVSPRGAGWLFGSKVAREFNHVNGLNLIARAHQLVMEGFKYHFPEKDVVTVWSAPNYCYRCGNVASVMKVDEDLEPTFKIFSAVPDDYIRESTANHNNQRAGYFL AF52BC65E4E715EC AF52BC65E4E715EC icp22_ehv1k Transcriptional regulator ICP22 homolog IR4 IR4 protein Immediate-early protein IE68 In vitro host-range factor ORF S1-2 protein protein ehv1k Equine herpesvirus 1 (strain Kentucky A) EHV-1 Equine abortion virus MPHGQPCGACDGSCRMAQRGTPSTSPLIPSLTPSPPAGDPSPRSSQRIDAVRVPARLPGGSDHPEYGMPLSPRALRPYLARGPGAFCAPPWRPDVNRLAGDVNRLFRGISTSSIHVTEDSRTLRRALLDFYAMGYTHTRPTLECWQSLLQLLPEQSFPLRATLRALNSEDRYEQRFLEPPSDPPNTLFGEECDVSGDESPSEEEEEDEASGESSVSEFSPEEETASSEYDSFSDVGEDDSSCTGKWSSSESESDSESDAPTNNHHPTTRASAAKKRRKRQPPKGERPTKSARR 3D9E89D028276D7F gac1_yeast Serine/threonine-protein phosphatase 1 regulatory subunit GAC1 YOR178C GAC1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVIQTATTLSPAKARPSFPHNDLIKSMSDSLISRPTHPPIRKLKSSLKISHPEPISRSKSEIFCTSPEKNVRFAIELTTVKRFDKNAEPSSISNENSPTLSPVDSNTAADDVQLFNNEDCWFNDSSLVTNLLKNEKKFRYMNSLNNMFKLDLYDSEDEDDIDEHINSQAEYGYTYNSLSTRGKTSENKSATSSLATQATNICDWKLHCTDLVPFKIAPPLFTKTLSASDLQGQLTKYLNGQNVKLHSLTQLGDDSSKITGLVYVKNLSFEKYLEIKFTFNSWRDIHYVTANFNRTINSNVDEFKFTIDLNSLKYILLIKRIITMEKNTSSCPLNIELCCRYDVNNETYYDNNNGKNYHLFMTTFKKGGETKEKIPVVVEPASQTDAAMSPKEMKARFVSSNPTLSRFLPQSRKFSEDTDYYNTSPLKHLYHNDTTSWVKPKRLNVVLDKLENATPPPPSSALANDTARTGKITKDKNNVLAPPTASNSIDLPILGSQHQSLYSGSSSYSSSSSSISSSLSFASSNNSSTNSSSASCSFPLTELDNFDYANLYEPNDTFTTANLFNHSLNSLMPEISTPSFFGGFRNENTINNNDSKNLVTSLEDSYEDKQSVITDTTMDENNKTSTINNSTDTLIKPSKENGTVKENKSSANSTSAPSSSQNRASTILNDHSNGKSDLKYVNYQSLLDSHCFYNHPSSPNLQSTSFSSAAPFSGISQASDIFDYENEDSDSNQIAGEIDNNSFPPHFYLDEDDKSACLSDDALIDHHRNTNPFINTFSSSPPILSQEVDRWRL 1880E966A6480D80 1880E966A6480D80 icp4_ehv1k Major viral transcription factor IE 155 kDa immediate-early protein protein protein ehv1k Equine herpesvirus 1 (strain Kentucky A) EHV-1 Equine abortion virus MASQRSDFAPDLYDFIESNDFGEDPLIRAASAAEEGFTQPAAPDLLYGSQNMFGVDDAPLSTPVVVIPPPSPAPEPRGGKAKRSPSAAGSGGPPTPAAAAQPASPAPSPAPGLAAMLKMVHSSVAPGNGRRATGSSSPGGGDAADPVALDSDTETCPGSPQPEFPSSASPGGGSPAPRVRSISISSSSSSSSSMDEDDQADGAGASSSSSSSSDDSDSDEGGEEETPRPRHSQNAAKTPSAAGSPGPSSGGDRPAAGAATPKSCRSGAASPGAPAPAPASAPAPSRPGGGLLPPGARILEYLEGVREANLAKTLERPEPPAGMASPPGRSPHRLPKDQRPKSALAGASKRKRANPRPRPQTQTQAPAEEAPQTAVWDLLDMNSSQATGAAAAAASAPAAASCAPGVYQREPLLTPSGDPWPGSDPPPMGRVRYGGTGDSRDGLWDDPEIVLAASRYAEAQAPVPVFVPEMGDSTKQYNALVRMVFESREAMSWLQNSKLSGQDQNLAQFCQKFIHAPRGHGSFITGSVANPLPHIGDAMAAGNALWALPHAAASVAMSRRYDRTQKSFILQSLRRAYADMAYPRDEAGRPDSLAAVAGCPAQAAAAAASQQQPEAPAPSVRVREAYTRVCAALGPRRKAAAAAAAPGTRAPRPSAFRLRELGDACVLACQAVFEALLRLRGGASAVPGLDPSEIPSPACPPEALCSNPAGLETAALSLYELRDLVERARLLGDSDPTHRLGSDELRLAVRAVLVVARTVAPLVRYNAEGARARASAWTVTQAVFSIPSLVGGMLGEAVSLLAPPTRSQQPSSSSPGGEPFSGSAAAEGSLQTLPPLWPTVPGKQSATVPSSHSQSPQHSQSGGGAGATTATCCRATQTNARSRGQQHQPQKARSPQAAASPAHLSQEAMPGSSSDDRAIHGRPRGKSGKRRSEPLEPAAQAGASASFSSSARGYDPSGPVDSPPAPKRRVATPGHQAPRALGPMPAEGPDRRGGFRRVPRGDCHTPRPSDAACAAYCPPELVAELIDNQLFPEAWRPALTFDPQALATIAARCSGPPARDGARLGELAASGPLRRRAAWMHQIPDPEDVKVVVLYSPLQDEDLLGGLPASRPGGSRREPLWSDLKGGLSALLAALGNRILTKRSHAWAGNWTGAPDVSALNAQGVLLLSTGDLAFTGCVEYLCLRLGSARRKLLVLDAVSTEDWPQDGPAISQYHIYMRAALTPRVACAVRWPGERHLSRAVLTSSTLFGPGLFARAEAAFARLYPDSAPLRLCRSSNVAYTVDTRAGERTRVPLAPREYRQRVLPDYDGCKDMRAQAEGLGFHDPDFEEGAAQSHRAANRWGLGAWLRPVYLACGRRGAGAVEPSELLIPELLSEFCRVALLEPDAEAEPLVLPITEAPRRRAPRVDWEPGFGSRSTSVLHMGATELCLPEPDDELEIDGAGDVELVVEHPGPSPGVAQALRRAPIKIEVVSDDEDGGDWCNPYLS 044E39A570608A6B icp22-icp22-1 3 8 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component physical association physical association 1 icp22-icp22-2 2 8 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait physical association physical association table 1 & 2 icp22-ie-1 2 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association table 1 & 2 ie-icp22-2 2 10 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association table 1 ie-ir4-1 2 10 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association table 1 icp22-ie-2 1 10 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 8 unspecified role unspecified role bait bait 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 2 ie-ir4-2 1 10 unspecified role unspecified role bait bait 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 8 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 2 ie-ir4-3 6 10 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ir2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 8 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 40 43 physical association physical association 3A & 8 ir4-ir4-1 6 8 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 3A & 7 ir4-ie-1 6 8 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 competitor competitor competitor unspecified role unspecified role 10 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 8B ir4-ie-2 5 10 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4 ie-ir4-4 5 10 unspecified role unspecified role bait bait 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4 ir4-ie-3 4 10 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 5 gac1-sit4-1 2 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association table 1 sit4-gac1-1 2 7 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association table 1